Rorug02G0318500

glycerol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007805
Physical Location & Seq
Forward (+)
38917922 .. 38920586
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0318500.1

Sequence Viewer

Length: 837 bp
ATGCCCTTCCCTCAGGACCGCCTGGACCTCCCTCTCGACGCCCTCATCGCCGAATCCAAGCAACAACGCCGTCACAACAGCCACGCTCACCAGCCACGAGGGCATAACGGTCATTTCGGCGGCGGAGGCTCCAACCGCAACGCCTACAGAACGGCGCCGTATCCCACTCTGCCGGAAATGCAAGGACAGCCTGGACCCAGAAGGATTGAGCTGCCATTGGCTTTGAGCCAAGCGATGAACAGTGAGGGAAGCACCAAGCTTTATATATCTAACTTGGACTATGATGTTACCAATCGAGATATAGAGTTGCTCTTCTCTGAGGTTGGTGAATTGGAAAGGCACTCAATCCATTATGATAAAAGTGGAAGATCTAAGGGAACAGCTGAAGTTGTCTATAAGCACTATTCAGATGCTCTAGCAGCAATCGAGAGATACAACAATCAGCATCTTGATGGAAAGAAAGTGGAAATTAAGCTTGTGGGACTCAATGTTGTTTCTCCTGTTCCTCTGCTTCTGCCTCCAAATACAAATTTCATGCAAGAACATCCAACTCCCGTCTTCCAGAGGCTAGGAAGAGCTGGTTCAAGGGGATCGTTTCATGGTCATGGTGGTGTTGGACTTGAAAGAGGCTGTGGACAGGTGAGAAATGGTGGTCCGAAGGTTACTACAGAATCTCAGCAATGGAAGAATGTGACTGTAGAAAATCAGCAAAGGAAGAATGTGATTACAGAATCTGTTAAAAGTAAGAAAACATTTTATGGAGATACAACTGTGACTTTTGCTGATCTGGATGCTGATTTGGAAAAATACCGCCTAAAATGTAGGCCGGTAAACTGA

Protein Analysis

278

Amino Acids

31.05

Weight (kDa)

9.22

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 87 - 155 9.8e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AciI CCGC 5 cut(s) 19, 120, 123, 136, 811
AclWI GGATC 1 cut(s) 598
AcsI RAATTY 1 cut(s) 529
AcuI CTGAAG 1 cut(s) 405
AcyI GRCGYC 2 cut(s) 39, 155
AgsI TTSAA 2 cut(s) 585, 623
AjnI CCWGG 2 cut(s) 21, 190
AluBI AGCT 5 cut(s) 211, 259, 383, 475, 578
AluI AGCT 5 cut(s) 211, 259, 383, 475, 578
AlwI GGATC 1 cut(s) 598
AlwNI CAGNNNCTG 1 cut(s) 734
AoxI GGCC 1 cut(s) 824
ApeKI GCWGC 2 cut(s) 211, 419
ApoI RAATTY 1 cut(s) 529
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 4 cut(s) 16, 25, 194, 653
AsuHPI GGTGA 3 cut(s) 80, 338, 652
AvaII GGWCC 4 cut(s) 16, 25, 194, 653
AxyI CCTNAGG 1 cut(s) 12
BaeI ACNNNNGTAYC 2 cut(s) 756, 789
BanI GGYRCC 1 cut(s) 154
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 550
BbvI GCAGC 2 cut(s) 198, 431
BccI CCATC 1 cut(s) 446
BceAI ACGGC 3 cut(s) 54, 142, 168
BciT130I CCWGG 2 cut(s) 23, 192
BciVI GTATCC 1 cut(s) 171
BfaI CTAG 2 cut(s) 416, 569
BfmI CTRYAG 3 cut(s) 145, 666, 696
BfoI RGCGCY 1 cut(s) 158
BfuI GTATCC 1 cut(s) 171
BglI GCCNNNNNGGC 1 cut(s) 100
BglII AGATCT 1 cut(s) 368
BisI GCNGC 3 cut(s) 121, 212, 420
BlsI GCNGC 3 cut(s) 122, 213, 421
Bme1390I CCNGG 2 cut(s) 23, 192
Bme18I GGWCC 4 cut(s) 16, 25, 194, 653
BmgT120I GGNCC 4 cut(s) 16, 25, 194, 653
BmiI GGNNCC 3 cut(s) 130, 156, 196
BmrFI CCNGG 2 cut(s) 23, 192
BmsI GCATC 3 cut(s) 400, 454, 781
BpiI GAAGAC 1 cut(s) 550
BsaHI GRCGYC 2 cut(s) 39, 155
Bse118I RCCGGY 1 cut(s) 826
Bse21I CCTNAGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 686
BseBI CCWGG 2 cut(s) 23, 192
BseGI GGATG 2 cut(s) 544, 796
BseMI GCAATG 1 cut(s) 686
BseMII CTCAG 3 cut(s) 26, 309, 689
BseXI GCAGC 2 cut(s) 198, 431
BshFI GGCC 1 cut(s) 826
BshNI GGYRCC 1 cut(s) 154
BsiSI CCGG 2 cut(s) 173, 827
BslFI GGGAC 1 cut(s) 495
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 1 cut(s) 826
Bsp143I GATC 3 cut(s) 368, 590, 784
BspACI CCGC 5 cut(s) 19, 120, 123, 136, 811
BspANI GGCC 1 cut(s) 826
BspCNI CTCAG 3 cut(s) 25, 310, 688
BspLI GGNNCC 3 cut(s) 130, 156, 196
BspPI GGATC 1 cut(s) 598
BspQI GCTCTTC 2 cut(s) 317, 568
BspT107I GGYRCC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 826
BssAI RCCGGY 1 cut(s) 826
BssMI GATC 3 cut(s) 368, 590, 784
BssNI GRCGYC 2 cut(s) 39, 155
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 23, 192
Bst4CI ACNGT 4 cut(s) 110, 242, 697, 772
Bst6I CTCTTC 2 cut(s) 317, 568
BstACI GRCGYC 2 cut(s) 39, 155
BstDEI CTNAG 4 cut(s) 12, 318, 372, 675
BstF5I GGATG 2 cut(s) 544, 796
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 3 cut(s) 371, 593, 787
BstMBI GATC 3 cut(s) 368, 590, 784
BstMWI GCNNNNNNNGC 7 cut(s) 47, 100, 126, 135, 178, 187, 419
BstNI CCWGG 2 cut(s) 23, 192
BstSCI CCNGG 2 cut(s) 21, 190
BstSFI CTRYAG 3 cut(s) 145, 666, 696
BstV1I GCAGC 2 cut(s) 198, 431
BstV2I GAAGAC 1 cut(s) 550
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
Bsu36I CCTNAGG 1 cut(s) 12
BsuI GTATCC 1 cut(s) 171
BsuRI GGCC 1 cut(s) 826
BtgZI GCGATG 2 cut(s) 31, 248
BtsCI GGATG 2 cut(s) 544, 796
BtsIMutI CAGTG 1 cut(s) 247
CaiI CAGNNNCTG 1 cut(s) 734
CfoI GCGC 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 826
Cfr13I GGNCC 4 cut(s) 16, 25, 194, 653
CseI GACGC 1 cut(s) 47
CviAII CATG 3 cut(s) 535, 599, 605
DdeI CTNAG 4 cut(s) 12, 318, 372, 675
DinI GGCGCC 1 cut(s) 156
DpnI GATC 3 cut(s) 370, 592, 786
DpnII GATC 3 cut(s) 368, 590, 784
Eam1104I CTCTTC 2 cut(s) 317, 568
EarI CTCTTC 2 cut(s) 317, 568
EciI GGCGGA 1 cut(s) 138
Eco47I GGWCC 4 cut(s) 16, 25, 194, 653
Eco57I CTGAAG 1 cut(s) 405
Eco81I CCTNAGG 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 21, 190
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
FaeI CATG 3 cut(s) 538, 602, 608
FaqI GGGAC 1 cut(s) 495
FatI CATG 3 cut(s) 534, 598, 604
Fnu4HI GCNGC 3 cut(s) 121, 212, 420
FokI GGATG 2 cut(s) 531, 803
Fsp4HI GCNGC 3 cut(s) 121, 212, 420
FspBI CTAG 2 cut(s) 416, 569
GlaI GCGC 1 cut(s) 156
GluI GCNGC 3 cut(s) 121, 212, 420
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 1 cut(s) 826
HapII CCGG 2 cut(s) 173, 827
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 2 cut(s) 39, 155
Hin1II CATG 3 cut(s) 538, 602, 608
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HindIII AAGCTT 2 cut(s) 257, 473
HinfI GANTC 4 cut(s) 53, 483, 671, 731
HpaII CCGG 2 cut(s) 173, 827
HphI GGTGA 3 cut(s) 80, 338, 652
Hpy166II GTNNAC 2 cut(s) 635, 832
Hpy188I TCNGA 3 cut(s) 319, 409, 657
Hpy188III TCNNGA 7 cut(s) 14, 35, 296, 427, 449, 562, 788
Hpy8I GTNNAC 2 cut(s) 635, 832
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 16, 195, 652
HpyCH4III ACNGT 4 cut(s) 110, 242, 697, 772
HpyCH4V TGCA 2 cut(s) 181, 538
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 100, 126, 135, 178, 187, 419
HpyF3I CTNAG 4 cut(s) 12, 318, 372, 675
Hsp92I GRCGYC 2 cut(s) 39, 155
Hsp92II CATG 3 cut(s) 538, 602, 608
HspAI GCGC 1 cut(s) 155
KasI GGCGCC 1 cut(s) 154
Kzo9I GATC 3 cut(s) 368, 590, 784
LguI GCTCTTC 2 cut(s) 317, 568
LmnI GCTCC 1 cut(s) 134
Lsp1109I GCAGC 2 cut(s) 198, 431
LweI GCATC 3 cut(s) 400, 454, 781
MaeI CTAG 2 cut(s) 416, 569
MaeIII GTNAC 5 cut(s) 71, 286, 661, 691, 772
MalI GATC 3 cut(s) 370, 592, 786
MboI GATC 3 cut(s) 368, 590, 784
MboII GAAGA 6 cut(s) 304, 378, 550, 585, 697, 727
MflI RGATCY 1 cut(s) 368
MluCI AATT 3 cut(s) 329, 468, 529
Mly113I GGCGCC 1 cut(s) 155
MlyI GAGTC 1 cut(s) 477
MmeI TCCRAC 3 cut(s) 156, 572, 595
MseI TTAA 2 cut(s) 471, 738
MslI CAYNNNNRTG 3 cut(s) 450, 603, 609
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 173, 827
MspR9I CCNGG 2 cut(s) 23, 192
MvaI CCWGG 2 cut(s) 23, 192
MwoI GCNNNNNNNGC 7 cut(s) 47, 100, 126, 135, 178, 187, 419
NarI GGCGCC 1 cut(s) 155
NdeII GATC 3 cut(s) 368, 590, 784
NlaIII CATG 3 cut(s) 538, 602, 608
NlaIV GGNNCC 3 cut(s) 130, 156, 196
NmuCI GTSAC 3 cut(s) 71, 691, 772
PciSI GCTCTTC 2 cut(s) 317, 568
PfeI GAWTC 3 cut(s) 53, 671, 731
PkrI GCNGC 3 cut(s) 122, 213, 421
PleI GAGTC 1 cut(s) 477
PluTI GGCGCC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 477
Psp6I CCWGG 2 cut(s) 21, 190
PspGI CCWGG 2 cut(s) 21, 190
PspN4I GGNNCC 3 cut(s) 130, 156, 196
PspPI GGNCC 4 cut(s) 16, 25, 194, 653
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 1 cut(s) 368
PvuII CAGCTG 1 cut(s) 383
RseI CAYNNNNRTG 3 cut(s) 450, 603, 609
SapI GCTCTTC 2 cut(s) 317, 568
SaqAI TTAA 2 cut(s) 471, 738
SatI GCNGC 3 cut(s) 121, 212, 420
Sau3AI GATC 3 cut(s) 368, 590, 784
Sau96I GGNCC 4 cut(s) 16, 25, 194, 653
SchI GAGTC 1 cut(s) 477
ScrFI CCNGG 2 cut(s) 23, 192
SetI ASST 9 cut(s) 30, 213, 261, 324, 385, 477, 580, 642, 663
SfaNI GCATC 3 cut(s) 400, 454, 781
SfcI CTRYAG 3 cut(s) 145, 666, 696
SfoI GGCGCC 1 cut(s) 156
SinI GGWCC 4 cut(s) 16, 25, 194, 653
SmiMI CAYNNNNRTG 3 cut(s) 450, 603, 609
Sse9I AATT 3 cut(s) 329, 468, 529
SsiI CCGC 5 cut(s) 19, 120, 123, 136, 811
SspDI GGCGCC 1 cut(s) 154
SspMI CTAG 2 cut(s) 416, 569
StyD4I CCNGG 2 cut(s) 21, 190
TaaI ACNGT 4 cut(s) 110, 242, 697, 772
TaqI TCGA 3 cut(s) 36, 295, 426
TasI AATT 3 cut(s) 329, 468, 529
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 3 cut(s) 53, 671, 731
Tru1I TTAA 2 cut(s) 471, 738
Tru9I TTAA 2 cut(s) 471, 738
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 3 cut(s) 71, 691, 772
TseI GCWGC 2 cut(s) 211, 419
Tsp45I GTSAC 3 cut(s) 71, 691, 772
TspDTI ATGAA 3 cut(s) 251, 523, 587
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 4 cut(s) 16, 25, 194, 653
XapI RAATTY 1 cut(s) 529
XspI CTAG 2 cut(s) 416, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.