Rh6DG231400

glycerol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0007805
Physical Location & Seq
Forward (+)
41263042 .. 41265253
2212 bp
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UTR
Exon/CDS
Intron
Rh6DG231400.1

Sequence Viewer

Length: 453 bp
ATGAGCATAGTGCGTGATGCATTGGAGACATCCGTTACATGTACCCCTCCCATGACACCACCGGTTAAATTCCACATGAAGGTTCATTTCGCTACTTTCAGATGGGTGGAGCATGATCCAATGGAGATATTGGAGAGTGTGACACTTTGTATGGCTATGGCTCTAGACAAAGCCACAGCCAACGGGCACAACATCGATGGTAGATTGAAGGCGATTGGGTTGACCAATCAGAGAGAGATCACTCTCGTTTGGAGCAAATCCACTGGCTGTCCTCTTTACAATGATGTTGTTTGGATGGATGCTGGTACCAGTTTTGTTTGCAGGGGATGCTCTGTTTGGAACTATAGACACTTGGTTAATGTGGAAACTGTTGGTGTCATGGGAGGGGTACATGTCACAGATGTCTCCAACGCATCACGCACTATGCTCATGAATCTCAAGACTCTTGAGTGA

Protein Analysis

150

Amino Acids

16.69

Weight (kDa)

6.58

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FGGY_N PF00370 26 - 109 4.4e-12 FGGY family of carbohydrate kinases, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 305
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 1 cut(s) 68
AfaI GTAC 3 cut(s) 43, 307, 390
AfiI CCNNNNNNNGG 1 cut(s) 79
AflIII ACRYGT 2 cut(s) 38, 391
AgeI ACCGGT 1 cut(s) 61
AgsI TTSAA 1 cut(s) 208
Alw26I GTCTC 2 cut(s) 20, 409
AlwI GGATC 1 cut(s) 110
ApoI RAATTY 1 cut(s) 68
AsiGI ACCGGT 1 cut(s) 61
Asp718I GGTACC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 305
BccI CCATC 3 cut(s) 96, 191, 289
BcoDI GTCTC 2 cut(s) 20, 409
BfaI CTAG 1 cut(s) 164
BfmI CTRYAG 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 307
BmsI GCATC 4 cut(s) 7, 289, 317, 422
BpuEI CTTGAG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 195
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 1 cut(s) 79
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 268, 309
BseCI ATCGAT 1 cut(s) 195
BseGI GGATG 4 cut(s) 29, 300, 304, 332
BseLI CCNNNNNNNGG 1 cut(s) 79
BseNI ACTGG 2 cut(s) 268, 309
BseSI GKGCMC 1 cut(s) 189
BshNI GGYRCC 1 cut(s) 305
BshTI ACCGGT 1 cut(s) 61
BshVI ATCGAT 1 cut(s) 195
BsiSI CCGG 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 2 cut(s) 20, 409
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 2 cut(s) 115, 237
BspDI ATCGAT 1 cut(s) 195
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 307
BspPI GGATC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 2 cut(s) 268, 309
BssAI RCCGGY 1 cut(s) 61
BssMI GATC 2 cut(s) 115, 237
Bst4CI ACNGT 1 cut(s) 370
BstAPI GCANNNNNTGC 1 cut(s) 327
BstF5I GGATG 4 cut(s) 29, 300, 304, 332
BstKTI GATC 2 cut(s) 118, 240
BstMAI GTCTC 2 cut(s) 20, 409
BstMBI GATC 2 cut(s) 115, 237
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstNSI RCATGY 2 cut(s) 42, 395
BstSFI CTRYAG 1 cut(s) 343
BstSLI GKGCMC 1 cut(s) 189
Bsu15I ATCGAT 1 cut(s) 195
BsuTUI ATCGAT 1 cut(s) 195
BtsCI GGATG 4 cut(s) 29, 300, 304, 332
BtsIMutI CAGTG 1 cut(s) 261
CciI TCATGA 1 cut(s) 429
Cfr10I RCCGGY 1 cut(s) 61
ClaI ATCGAT 1 cut(s) 195
Csp6I GTAC 3 cut(s) 42, 306, 389
CspAI ACCGGT 1 cut(s) 61
CviAII CATG 7 cut(s) 39, 52, 76, 113, 379, 392, 430
CviJI RGCY 5 cut(s) 155, 161, 173, 179, 267
CviKI_1 RGCY 5 cut(s) 155, 161, 173, 179, 267
CviQI GTAC 3 cut(s) 42, 306, 389
DpnI GATC 2 cut(s) 117, 239
DpnII GATC 2 cut(s) 115, 237
EcoT22I ATGCAT 1 cut(s) 22
FaeI CATG 7 cut(s) 42, 55, 79, 116, 382, 395, 433
FatI CATG 7 cut(s) 38, 51, 75, 112, 378, 391, 429
FokI GGATG 4 cut(s) 16, 307, 311, 339
FspBI CTAG 1 cut(s) 164
HapII CCGG 1 cut(s) 62
Hin1II CATG 7 cut(s) 42, 55, 79, 116, 382, 395, 433
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 2 cut(s) 433, 442
HpaII CCGG 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 222
Hpy188I TCNGA 2 cut(s) 101, 231
Hpy188III TCNNGA 4 cut(s) 164, 430, 439, 446
Hpy8I GTNNAC 1 cut(s) 222
HpyAV CCTTC 2 cut(s) 73, 202
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4V TGCA 2 cut(s) 20, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
Hsp92II CATG 7 cut(s) 42, 55, 79, 116, 382, 395, 433
KpnI GGTACC 1 cut(s) 309
Kzo9I GATC 2 cut(s) 115, 237
LmnI GCTCC 2 cut(s) 109, 252
LpnPI CCDG 5 cut(s) 75, 249, 288, 307, 322
LweI GCATC 4 cut(s) 7, 289, 317, 422
MaeI CTAG 1 cut(s) 164
MaeIII GTNAC 3 cut(s) 34, 139, 394
MalI GATC 2 cut(s) 117, 239
MboI GATC 2 cut(s) 115, 237
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 1 cut(s) 68
MlyI GAGTC 1 cut(s) 436
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 3 cut(s) 57, 282, 377
Mph1103I ATGCAT 1 cut(s) 22
MseI TTAA 2 cut(s) 66, 357
MspI CCGG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 327
NdeII GATC 2 cut(s) 115, 237
NlaIII CATG 7 cut(s) 42, 55, 79, 116, 382, 395, 433
NlaIV GGNNCC 1 cut(s) 307
NmuCI GTSAC 2 cut(s) 139, 394
NsiI ATGCAT 1 cut(s) 22
NspI RCATGY 2 cut(s) 42, 395
PagI TCATGA 1 cut(s) 429
PciI ACATGT 2 cut(s) 38, 391
PfeI GAWTC 1 cut(s) 433
PinAI ACCGGT 1 cut(s) 61
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PscI ACATGT 2 cut(s) 38, 391
PspN4I GGNNCC 1 cut(s) 307
RsaI GTAC 3 cut(s) 43, 307, 390
RsaNI GTAC 3 cut(s) 42, 306, 389
SaqAI TTAA 2 cut(s) 66, 357
Sau3AI GATC 2 cut(s) 115, 237
SchI GAGTC 1 cut(s) 436
SduI GDGCHC 1 cut(s) 189
SetI ASST 1 cut(s) 84
SfaNI GCATC 4 cut(s) 7, 289, 317, 422
SfcI CTRYAG 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 437, 446
SmoI CTYRAG 2 cut(s) 437, 446
Sse9I AATT 1 cut(s) 68
SspMI CTAG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 370
TaqI TCGA 1 cut(s) 195
TasI AATT 1 cut(s) 68
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 2 cut(s) 66, 357
Tru9I TTAA 2 cut(s) 66, 357
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 2 cut(s) 139, 394
Tsp45I GTSAC 2 cut(s) 139, 394
TspDTI ATGAA 3 cut(s) 74, 92, 446
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 268
XapI RAATTY 1 cut(s) 68
XbaI TCTAGA 1 cut(s) 163
XceI RCATGY 2 cut(s) 42, 395
XspI CTAG 1 cut(s) 164
Zsp2I ATGCAT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.