Rorug02G0584500

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005792
Physical Location & Seq
Reverse (-)
70749921 .. 70753311
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0584500.1

Sequence Viewer

Length: 942 bp
ATGTTGATAACCGTGTCACAGGACGGCTCCGGTGACCACCGCACCGTACAGGAGGCCATCGACGCCGTGCCTCTCTGCAACACGTGTCGCACTGTGATTCGTATAGCTCCGGGCATCTACCGGCAGCCGGTGTATGTCCCCAAGACCAAGAACCTCATCACACTCGCGGGTCTGCGACCGGAGGATACTGTGCTGACGTGGAACAACACCGCCACCAAAATCGAGCACCACCAGGCTTCTAGGGTGATTGGGACTGGGACTTTTGGATGTGGGAGTACAATTGTGGAAGGAGAGGACTTCATTGCTGAGAATATCACTTTTGAGAACTCTTCTCCTGAGGGTTCGGGCCAAGCTGTGGCAATTAGAGTAACAGCTGATCGGTGCGCCTTCTATAACTGCAGATTCCTTGGATGGCAGGATACCCTGTACTTGCATTATGGAAAGCAGTATTTGAAAGATTGTTATATTGAAGGAAGTGTAGACTTCATTTTCGGTAATAGCACAGCTCTTTTGGAGCATTGTCATATCCACTGCAAGTCAGCAGGTTTCATAACTGCGCAAAGCAGGAAATCATCCCAGGAGACTACAGGATATGTGTTCCTAAGGTGTATAATAACTGGAAATGGAGGTGCTCCATATGCATTTCTGGGACGACCATGGGGACCTTTTGGAAGAGTGGTCTTTGCATTTACATATATGGATGCATGCATCAAACATGTAGGCTGGAATAATTGGGGTAAAACAGAGAATGAAAGAAGCGCTTGCTTTTACGAATACAGGTGTTTTGGACCGGGTAGTTGCCCATCAAAACGAGTGACATGGGCTAGAGAACTGGTAGATGAGGAAGCAGACCAGTTCCTCAAGCATGGTTACATCGACCCAGACCCCCAAAGACCTTGGCTTGCTCAGAGAATGGCCCTAAGAATACCATATTCTGCATAG

Protein Analysis

313

Amino Acids

35.04

Weight (kDa)

7.07

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 3 - 293 2e-70 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 558
Acc36I ACCTGC 1 cut(s) 533
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 1 cut(s) 480
AccII CGCG 1 cut(s) 167
AciI CCGC 3 cut(s) 40, 167, 210
AcvI CACGTG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 3 cut(s) 48, 277, 428
AfeI AGCGCT 1 cut(s) 760
AfiI CCNNNNNNNGG 2 cut(s) 127, 355
AflIII ACRYGT 3 cut(s) 81, 83, 715
AgsI TTSAA 2 cut(s) 454, 470
AjiI CACGTC 1 cut(s) 198
AjnI CCWGG 2 cut(s) 231, 576
AluBI AGCT 4 cut(s) 107, 353, 374, 506
AluI AGCT 4 cut(s) 107, 353, 374, 506
Alw21I GWGCWC 2 cut(s) 228, 634
Alw26I GTCTC 1 cut(s) 575
Aor51HI AGCGCT 1 cut(s) 760
AoxI GGCC 3 cut(s) 54, 346, 915
ApeKI GCWGC 1 cut(s) 124
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AspLEI GCGC 3 cut(s) 386, 559, 761
AspS9I GGNCC 4 cut(s) 346, 662, 788, 916
AsuC2I CCSGG 2 cut(s) 111, 792
AsuHPI GGTGA 2 cut(s) 44, 256
AvaII GGWCC 2 cut(s) 662, 788
AxyI CCTNAGG 2 cut(s) 336, 602
BbrPI CACGTG 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 228, 634
BbvI GCAGC 1 cut(s) 136
BccI CCATC 3 cut(s) 65, 405, 811
BceAI ACGGC 2 cut(s) 40, 50
BciT130I CCWGG 2 cut(s) 233, 578
BciVI GTATCC 2 cut(s) 178, 412
BcnI CCSGG 2 cut(s) 111, 792
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 2 cut(s) 240, 825
BfmI CTRYAG 2 cut(s) 397, 585
BfoI RGCGCY 1 cut(s) 762
BfuAI ACCTGC 1 cut(s) 533
BfuI GTATCC 2 cut(s) 178, 412
BisI GCNGC 1 cut(s) 125
BlsI GCNGC 1 cut(s) 126
Bme1390I CCNGG 4 cut(s) 111, 233, 578, 792
Bme18I GGWCC 2 cut(s) 662, 788
BmgBI CACGTC 1 cut(s) 198
BmgT120I GGNCC 4 cut(s) 346, 662, 788, 916
BmiI GGNNCC 2 cut(s) 28, 663
BmrFI CCNGG 4 cut(s) 111, 233, 578, 792
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 3 cut(s) 123, 691, 717
BmuI ACTGGG 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 845
BpuMI CCSGG 2 cut(s) 111, 792
BsaAI YACGTR 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 4 cut(s) 406, 576, 656, 896
BsaWI WCCGGW 2 cut(s) 29, 178
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 355
Bse118I RCCGGY 2 cut(s) 120, 127
Bse1I ACTGG 4 cut(s) 259, 622, 837, 853
Bse21I CCTNAGG 2 cut(s) 336, 602
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 233, 578
BseDI CCNNGG 4 cut(s) 406, 576, 656, 896
BseGI GGATG 4 cut(s) 272, 416, 572, 706
BseLI CCNNNNNNNGG 2 cut(s) 127, 355
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 3 cut(s) 297, 327, 920
BseNI ACTGG 4 cut(s) 259, 622, 837, 853
BseXI GCAGC 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 167
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 3 cut(s) 56, 348, 917
BsiEI CGRYCG 1 cut(s) 179
BsiHKAI GWGCWC 2 cut(s) 228, 634
BsiSI CCGG 6 cut(s) 30, 110, 121, 128, 179, 791
BslFI GGGAC 5 cut(s) 122, 265, 271, 663, 675
BslI CCNNNNNNNGG 2 cut(s) 127, 355
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 5 cut(s) 122, 265, 271, 663, 675
BsnI GGCC 3 cut(s) 56, 348, 917
Bsp1286I GDGCHC 2 cut(s) 228, 634
Bsp143I GATC 1 cut(s) 376
Bsp19I CCATGG 1 cut(s) 656
BspACI CCGC 3 cut(s) 40, 167, 210
BspANI GGCC 3 cut(s) 56, 348, 917
BspCNI CTCAG 3 cut(s) 298, 328, 919
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 2 cut(s) 28, 663
BspMAI CTGCAG 1 cut(s) 401
BspMI ACCTGC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 300
BsrFI RCCGGY 2 cut(s) 120, 127
BsrI ACTGG 4 cut(s) 259, 622, 837, 853
BssAI RCCGGY 2 cut(s) 120, 127
BssECI CCNNGG 4 cut(s) 406, 576, 656, 896
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 3 cut(s) 406, 656, 896
Bst2UI CCWGG 2 cut(s) 233, 578
Bst4CI ACNGT 4 cut(s) 13, 46, 94, 190
Bst6I CTCTTC 2 cut(s) 334, 667
BstACI GRCGYC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 706, 763, 903
BstDEI CTNAG 5 cut(s) 306, 336, 602, 906, 920
BstDSI CCRYGG 1 cut(s) 656
BstEII GGTNACC 1 cut(s) 32
BstF5I GGATG 4 cut(s) 272, 416, 572, 706
BstFNI CGCG 1 cut(s) 167
BstH2I RGCGCY 1 cut(s) 762
BstHHI GCGC 3 cut(s) 386, 559, 761
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 1 cut(s) 376
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 2 cut(s) 62, 638
BstNI CCWGG 2 cut(s) 233, 578
BstNSI RCATGY 2 cut(s) 708, 719
BstPI GGTNACC 1 cut(s) 32
BstSCI CCNGG 4 cut(s) 109, 231, 576, 790
BstSFI CTRYAG 2 cut(s) 397, 585
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 1 cut(s) 136
Bsu36I CCTNAGG 2 cut(s) 336, 602
BsuI GTATCC 2 cut(s) 178, 412
BsuRI GGCC 3 cut(s) 56, 348, 917
BtgI CCRYGG 1 cut(s) 656
BtrI CACGTC 1 cut(s) 198
BtsCI GGATG 4 cut(s) 272, 416, 572, 706
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 90, 529
BveI ACCTGC 1 cut(s) 533
Cac8I GCNNGC 3 cut(s) 706, 763, 903
CfoI GCGC 3 cut(s) 386, 559, 761
Cfr10I RCCGGY 2 cut(s) 120, 127
Cfr13I GGNCC 4 cut(s) 346, 662, 788, 916
CseI GACGC 1 cut(s) 71
Csp6I GTAC 3 cut(s) 47, 276, 427
CviAII CATG 5 cut(s) 657, 705, 716, 819, 866
CviQI GTAC 3 cut(s) 47, 276, 427
DdeI CTNAG 5 cut(s) 306, 336, 602, 906, 920
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 334, 667
EarI CTCTTC 2 cut(s) 334, 667
Eco130I CCWWGG 3 cut(s) 406, 656, 896
Eco47I GGWCC 2 cut(s) 662, 788
Eco47III AGCGCT 1 cut(s) 760
Eco72I CACGTG 1 cut(s) 84
Eco81I CCTNAGG 2 cut(s) 336, 602
Eco91I GGTNACC 1 cut(s) 32
EcoO109I RGGNCCY 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 231, 576
EcoT14I CCWWGG 3 cut(s) 406, 656, 896
EcoT22I ATGCAT 3 cut(s) 643, 706, 710
ErhI CCWWGG 3 cut(s) 406, 656, 896
FaeI CATG 5 cut(s) 660, 708, 719, 822, 869
FalI AAGNNNNNCTT 2 cut(s) 745, 777
FaqI GGGAC 5 cut(s) 122, 265, 271, 663, 675
FatI CATG 5 cut(s) 656, 704, 715, 818, 865
FauI CCCGC 1 cut(s) 160
FauNDI CATATG 1 cut(s) 637
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 1 cut(s) 125
FokI GGATG 4 cut(s) 279, 423, 559, 713
Fsp4HI GCNGC 1 cut(s) 125
FspBI CTAG 2 cut(s) 240, 825
FspI TGCGCA 1 cut(s) 558
GlaI GCGC 3 cut(s) 385, 558, 760
GluI GCNGC 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 762
HaeIII GGCC 3 cut(s) 56, 348, 917
HapII CCGG 6 cut(s) 30, 110, 121, 128, 179, 791
HgaI GACGC 1 cut(s) 71
HhaI GCGC 3 cut(s) 386, 559, 761
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 5 cut(s) 660, 708, 719, 822, 869
Hin6I GCGC 3 cut(s) 384, 557, 759
HinP1I GCGC 3 cut(s) 384, 557, 759
HinfI GANTC 2 cut(s) 97, 402
HpaII CCGG 6 cut(s) 30, 110, 121, 128, 179, 791
HphI GGTGA 2 cut(s) 44, 256
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 1 cut(s) 909
Hpy188III TCNNGA 1 cut(s) 335
Hpy8I GTNNAC 1 cut(s) 481
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 3 cut(s) 281, 397, 464
HpyCH4III ACNGT 4 cut(s) 13, 46, 94, 190
HpyCH4IV ACGT 2 cut(s) 83, 197
HpyCH4V TGCA 9 cut(s) 78, 399, 433, 534, 641, 686, 704, 708, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 638
HpyF3I CTNAG 5 cut(s) 306, 336, 602, 906, 920
HpySE526I ACGT 2 cut(s) 83, 197
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 5 cut(s) 660, 708, 719, 822, 869
HspAI GCGC 3 cut(s) 384, 557, 759
Kzo9I GATC 1 cut(s) 376
LmnI GCTCC 4 cut(s) 32, 112, 514, 637
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 3 cut(s) 123, 691, 717
MaeI CTAG 2 cut(s) 240, 825
MaeII ACGT 2 cut(s) 83, 197
MaeIII GTNAC 5 cut(s) 15, 32, 367, 814, 869
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 2 cut(s) 321, 684
MfeI CAATTG 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 228, 634
MluCI AATT 3 cut(s) 279, 360, 730
MnlI CCTC 9 cut(s) 46, 81, 164, 175, 286, 331, 620, 835, 869
Mph1103I ATGCAT 3 cut(s) 643, 706, 710
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 6 cut(s) 30, 110, 121, 128, 179, 791
MspR9I CCNGG 4 cut(s) 111, 233, 578, 792
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 2 cut(s) 233, 578
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 2 cut(s) 62, 638
NciI CCSGG 2 cut(s) 111, 792
NcoI CCATGG 1 cut(s) 656
NdeI CATATG 1 cut(s) 637
NdeII GATC 1 cut(s) 376
NlaIII CATG 5 cut(s) 660, 708, 719, 822, 869
NlaIV GGNNCC 2 cut(s) 28, 663
NmuCI GTSAC 3 cut(s) 15, 32, 814
NsbI TGCGCA 1 cut(s) 558
NsiI ATGCAT 3 cut(s) 643, 706, 710
NspI RCATGY 2 cut(s) 708, 719
PaeI GCATGC 1 cut(s) 708
PciI ACATGT 1 cut(s) 715
PfeI GAWTC 2 cut(s) 97, 402
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 1 cut(s) 126
PmaCI CACGTG 1 cut(s) 84
PmlI CACGTG 1 cut(s) 84
Ppu21I YACGTR 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 662
PscI ACATGT 1 cut(s) 715
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 2 cut(s) 231, 576
PspCI CACGTG 1 cut(s) 84
PspEI GGTNACC 1 cut(s) 32
PspGI CCWGG 2 cut(s) 231, 576
PspN4I GGNNCC 2 cut(s) 28, 663
PspPI GGNCC 4 cut(s) 346, 662, 788, 916
PspPPI RGGWCCY 1 cut(s) 662
PstI CTGCAG 1 cut(s) 401
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 3 cut(s) 48, 277, 428
RsaNI GTAC 3 cut(s) 47, 276, 427
SatI GCNGC 1 cut(s) 125
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 4 cut(s) 346, 662, 788, 916
ScrFI CCNGG 4 cut(s) 111, 233, 578, 792
SduI GDGCHC 2 cut(s) 228, 634
SfaNI GCATC 3 cut(s) 123, 691, 717
SfcI CTRYAG 2 cut(s) 397, 585
SinI GGWCC 2 cut(s) 662, 788
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SphI GCATGC 1 cut(s) 708
Sse9I AATT 3 cut(s) 279, 360, 730
SsiI CCGC 3 cut(s) 40, 167, 210
SspMI CTAG 2 cut(s) 240, 825
StyD4I CCNGG 4 cut(s) 109, 231, 576, 790
StyI CCWWGG 3 cut(s) 406, 656, 896
TaaI ACNGT 4 cut(s) 13, 46, 94, 190
TaiI ACGT 2 cut(s) 86, 200
TaqI TCGA 3 cut(s) 60, 222, 876
TasI AATT 3 cut(s) 279, 360, 730
TatI WGTACW 2 cut(s) 275, 426
TfiI GAWTC 2 cut(s) 97, 402
TscAI CASTG 2 cut(s) 97, 536
TseFI GTSAC 3 cut(s) 15, 32, 814
TseI GCWGC 1 cut(s) 124
Tsp45I GTSAC 3 cut(s) 15, 32, 814
TspDTI ATGAA 4 cut(s) 289, 475, 538, 765
TspRI CASTG 2 cut(s) 97, 536
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 2 cut(s) 662, 788
XceI RCATGY 2 cut(s) 708, 719
XmiI GTMKAC 1 cut(s) 480
XspI CTAG 2 cut(s) 240, 825
Zsp2I ATGCAT 3 cut(s) 643, 706, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.