Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0005792

Physical Location & Seq

Genomic Coordinates Forward (+)

87971536 .. 87973217

Gene Structure 1682 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG664300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1263 bp

ATGTCCAAAGAAAGCAGGGATTCTAACCTGGGTGATTTTGGGCTAGGTTATCATACCAGCCCTCCAGAAAAGGTTAGGAGAAGATCAAGCATGAGCAGCAGCTTTGATGGAGATTCATTTTCGTCCAAGGAGCTCATGCAGCTTGATTGTGCCTTAACCTTATCACTGGGTACTAATATAGTGCCTAAACCGAAGGATGCAGATTATTGTTACTCAATCCCTCCCCTCATTGAAGAGGTGGAGGCGTTGATCTTGGCCCGGGCTCCCAGGTCTGAGTACTGGAAGTTCTTCTTGGTGAACAAGCGCTTCTTGGCACTTTTGAAGAGTGGAGAGCTGTACAAGATCAGGAGTGAACTCGGGCTCAAAGAGCTGTCTATTTTCGTGTCCCCTTGCGGGGAGAGAAAATGGTGGGAATTCGACAGGCAGTTCAAGTCCTGCAGGAAGCTCCCTATCTTACCCGCGGATGAAACTTTCATCCGCGGTGATAGGGAATCGCTTTGTGCAGGGACTCATCTGCTAGTCTCTGGCAGAGAAATTGAGGGGCTTGTTGTGTGGAGGTATGATATGGAAGAAAACATTTGGTCAAGGGGTCCTTCCATGATCAATCCAAGGTGCTTGTTTGCTTCTGCTACATGTGGTTCCTATGGATATGTAGCCGGTGGGTTTGGAATGGTAGCAACAGGTTGGGAAGTTATGGATTTCGCCGAGAGATACATTCCGCAGACTAAATCCTGGGAGCCTCTTCCAAATATGATAAAAAAGAGAAGGTATTGCTCTGGCTGTTACATGGACAACAAGTTTTATGTGATTGGCGGGAGAGACCCTGATGGAAGAGGTCTCAGTTGCGCAGAAGCCTTTGATATGGAGAAGAAGACATGGGAACTGATCCCAGACATGTTAGAAGTAGATGATGCTCAAGACCAAGCTTTGAATAACCAATCCCCGCCTCTCGTTGCGGTTGTGAACAACGAGCTCTACTCACTCGAAACCCGCACAAACGAGCTCAAGGTGCATATGAAGAGAAGCAAATCATGGAAGAAGTTGGGAACTGTTCCGGTGAGAGCTGATACTACTCTAGGATGGGGAATAGCTTTTAAGTCGCTGGGGAATGAGTTACTTGTCATACAATCTTCACAATATGGTCCTTCTCTGCTATACACTTGCTGCCCTAATCCAAATGCTGAGGAGTTGCAGTGGGAAGCTCTTGAACATGGCAAAAACCCTACCTGCTTCATCGTCAATTGTTCTGTCATGGCAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0019005 GO:0019222 GO:0019538 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031461 GO:0031625 GO:0032991 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0050789 GO:0051171 GO:0051246 GO:0051603 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_1 Kelch_6

Protein Analysis

420

Amino Acids

47.48

Weight (kDa)

6.0

Isoelectric Point (pI)

46.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Calicin	PF13964	184 - 299	3.1e-09	Calicin, beta-propeller domain
Kelch_FKB95	PF25210	242 - 298	1.2e-09	FKB95, Kelch-repeats domain
Kelch_1	PF01344	255 - 299	9.1e-13	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005792)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27150 AT3G27150 AT5G40680
fragaria_vesca	FvH4_6g53292 FvH4_6g53292 FvH4_6g53292 FvH4_6g53292 FvH4_6g53292
malus_domestica	MD09G1011800.v1.1 MD09G1011900.v1.1 MD17G1012500.v1.1
prunus_persica	Prupe.3G305200_v2.0.a1 Prupe.3G305200_v2.0.a1
pyrus_communis	pycom111g01010 pycom111g01020 pycom17g01000
rosa_chinensis	RchiOBHm_Chr2g0175451
rosa_laevigata	RLG00000022328
rosa_multiflora	Rmu_sc0003512.1_g000016
rosa_roxburghii	Rroxscaffold_2G00077320
rosa_rugosa	Rorug02G0584300 Rorug02G0584400 Rorug02G0584500
rosa_samantha	Rh2AG664300 Rh2BG675000 Rh2CG638300 Rh2DG689000
rosa_wichuraiana	Rw2G054440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	847
Acc36I	ACCTGC	1 cut(s)	1235
AccII	CGCG	2 cut(s)	461, 480
AclWI	GGATC	1 cut(s)	880
AcsI	RAATTY	1 cut(s)	413
AfaI	GTAC	3 cut(s)	172, 278, 338
AfeI	AGCGCT	1 cut(s)	305
AfiI	CCNNNNNNNGG	2 cut(s)	393, 394
AflIII	ACRYGT	2 cut(s)	632, 894
AgsI	TTSAA	5 cut(s)	233, 322, 430, 931, 1208
AjnI	CCWGG	3 cut(s)	27, 266, 731
AjuI	GAANNNNNNNTTGG	2 cut(s)	275, 307
Alw21I	GWGCWC	3 cut(s)	135, 975, 1005
Alw26I	GTCTC	3 cut(s)	526, 813, 842
AlwI	GGATC	1 cut(s)	880
Ama87I	CYCGRG	2 cut(s)	258, 356
Aor51HI	AGCGCT	1 cut(s)	305
AoxI	GGCC	1 cut(s)	255
ApeKI	GCWGC	5 cut(s)	96, 99, 139, 1164, 1256
ApoI	RAATTY	1 cut(s)	413
Asp700I	GAANNNNTTC	1 cut(s)	287
AspLEI	GCGC	2 cut(s)	306, 848
AspS9I	GGNCC	3 cut(s)	256, 590, 1142
AsuC2I	CCSGG	2 cut(s)	259, 260
AsuHPI	GGTGA	4 cut(s)	44, 307, 494, 1069
AvaI	CYCGRG	2 cut(s)	258, 356
AvaII	GGWCC	2 cut(s)	590, 1142
BanII	GRGCYC	5 cut(s)	135, 265, 363, 975, 1005
BbsI	GAAGAC	1 cut(s)	878
Bbv12I	GWGCWC	3 cut(s)	135, 975, 1005
BbvCI	CCTCAGC	1 cut(s)	1182
BbvI	GCAGC	4 cut(s)	108, 111, 151, 1151
BccI	CCATC	3 cut(s)	101, 821, 1074
BciT130I	CCWGG	3 cut(s)	29, 268, 733
BclI	TGATCA	1 cut(s)	600
BcnI	CCSGG	2 cut(s)	259, 260
BcoDI	GTCTC	3 cut(s)	526, 813, 842
BfaI	CTAG	3 cut(s)	44, 518, 1076
BfmI	CTRYAG	1 cut(s)	436
BfoI	RGCGCY	1 cut(s)	307
BfuAI	ACCTGC	1 cut(s)	1235
BisI	GCNGC	5 cut(s)	97, 100, 140, 1165, 1257
BlsI	GCNGC	5 cut(s)	98, 101, 141, 1166, 1258
BmcAI	AGTACT	1 cut(s)	278
Bme1390I	CCNGG	5 cut(s)	29, 259, 260, 268, 733
Bme18I	GGWCC	2 cut(s)	590, 1142
BmeT110I	CYCGRG	2 cut(s)	258, 356
BmgT120I	GGNCC	3 cut(s)	256, 590, 1142
BmiI	GGNNCC	4 cut(s)	264, 591, 640, 738
BmrFI	CCNGG	5 cut(s)	29, 259, 260, 268, 733
BmrI	ACTGGG	1 cut(s)	176
BmsI	GCATC	2 cut(s)	187, 901
BmuI	ACTGGG	1 cut(s)	176
BpiI	GAAGAC	1 cut(s)	878
BplI	GAGNNNNNCTC	2 cut(s)	962, 994
BpmI	CTGGAG	1 cut(s)	48
Bpu10I	CCTNAGC	1 cut(s)	1182
BpuEI	CTTGAG	2 cut(s)	900, 989
BpuMI	CCSGG	2 cut(s)	259, 260
BsaI	GGTCTC	2 cut(s)	813, 842
BsaJI	CCNNGG	8 cut(s)	28, 126, 258, 266, 459, 478, 608, 732
BsaWI	WCCGGW	1 cut(s)	1054
BsaXI	ACNNNNNCTCC	4 cut(s)	46, 76, 1178, 1208
Bsc4I	CCNNNNNNNGG	2 cut(s)	393, 394
Bse118I	RCCGGY	1 cut(s)	656
Bse1I	ACTGG	2 cut(s)	171, 284
BseBI	CCWGG	3 cut(s)	29, 268, 733
BseDI	CCNNGG	8 cut(s)	28, 126, 258, 266, 459, 478, 608, 732
BseGI	GGATG	4 cut(s)	202, 469, 474, 1085
BseLI	CCNNNNNNNGG	2 cut(s)	393, 394
BseMII	CTCAG	3 cut(s)	264, 853, 1173
BseNI	ACTGG	2 cut(s)	171, 284
BseRI	GAGGAG	1 cut(s)	1199
BseXI	GCAGC	4 cut(s)	108, 111, 151, 1151
BseYI	CCCAGC	1 cut(s)	1102
BsgI	GTGCAG	1 cut(s)	522
Bsh1236I	CGCG	2 cut(s)	461, 480
BshFI	GGCC	1 cut(s)	257
BsiHKAI	GWGCWC	3 cut(s)	135, 975, 1005
BsiHKCI	CYCGRG	2 cut(s)	258, 356
BsiSI	CCGG	3 cut(s)	259, 657, 1055
BslFI	GGGAC	2 cut(s)	370, 520
BslI	CCNNNNNNNGG	2 cut(s)	393, 394
BsmAI	GTCTC	3 cut(s)	526, 813, 842
BsmFI	GGGAC	2 cut(s)	370, 520
BsnI	GGCC	1 cut(s)	257
Bso31I	GGTCTC	2 cut(s)	813, 842
BsoBI	CYCGRG	2 cut(s)	258, 356
Bsp1286I	GDGCHC	5 cut(s)	135, 265, 363, 975, 1005
Bsp1407I	TGTACA	1 cut(s)	336
Bsp143I	GATC	5 cut(s)	83, 249, 342, 600, 885
BspANI	GGCC	1 cut(s)	257
BspCNI	CTCAG	3 cut(s)	265, 852, 1174
BspFNI	CGCG	2 cut(s)	461, 480
BspLI	GGNNCC	4 cut(s)	264, 591, 640, 738
BspMAI	CTGCAG	1 cut(s)	440
BspMI	ACCTGC	1 cut(s)	1235
BspPI	GGATC	1 cut(s)	880
BspTNI	GGTCTC	2 cut(s)	813, 842
BsrFI	RCCGGY	1 cut(s)	656
BsrGI	TGTACA	1 cut(s)	336
BsrI	ACTGG	2 cut(s)	171, 284
BssAI	RCCGGY	1 cut(s)	656
BssECI	CCNNGG	8 cut(s)	28, 126, 258, 266, 459, 478, 608, 732
BssMI	GATC	5 cut(s)	83, 249, 342, 600, 885
BssT1I	CCWWGG	2 cut(s)	126, 608
Bst2UI	CCWGG	3 cut(s)	29, 268, 733
Bst4CI	ACNGT	1 cut(s)	1051
Bst6I	CTCTTC	5 cut(s)	228, 317, 747, 826, 1013
BstAUI	TGTACA	1 cut(s)	336
BstDEI	CTNAG	3 cut(s)	273, 839, 1182
BstDSI	CCRYGG	2 cut(s)	459, 478
BstF5I	GGATG	4 cut(s)	202, 469, 474, 1085
BstFNI	CGCG	2 cut(s)	461, 480
BstH2I	RGCGCY	1 cut(s)	307
BstHHI	GCGC	2 cut(s)	306, 848
BstKTI	GATC	5 cut(s)	86, 252, 345, 603, 888
BstMAI	GTCTC	3 cut(s)	526, 813, 842
BstMBI	GATC	5 cut(s)	83, 249, 342, 600, 885
BstMWI	GCNNNNNNNGC	4 cut(s)	96, 139, 367, 1009
BstNI	CCWGG	3 cut(s)	29, 268, 733
BstNSI	RCATGY	2 cut(s)	636, 898
BstSCI	CCNGG	5 cut(s)	27, 257, 258, 266, 731
BstSFI	CTRYAG	1 cut(s)	436
BstUI	CGCG	2 cut(s)	461, 480
BstV1I	GCAGC	4 cut(s)	108, 111, 151, 1151
BstV2I	GAAGAC	1 cut(s)	878
BsuRI	GGCC	1 cut(s)	257
BtgI	CCRYGG	2 cut(s)	459, 478
BtsCI	GGATG	4 cut(s)	202, 469, 474, 1085
BtsI	GCAGTG	1 cut(s)	1199
BtsIMutI	CAGTG	2 cut(s)	164, 1199
BveI	ACCTGC	1 cut(s)	1235
CfoI	GCGC	2 cut(s)	306, 848
Cfr10I	RCCGGY	1 cut(s)	656
Cfr13I	GGNCC	3 cut(s)	256, 590, 1142
Cfr42I	CCGCGG	2 cut(s)	462, 481
Cfr9I	CCCGGG	1 cut(s)	258
Csp6I	GTAC	3 cut(s)	171, 277, 337
CviQI	GTAC	3 cut(s)	171, 277, 337
DdeI	CTNAG	3 cut(s)	273, 839, 1182
DpnI	GATC	5 cut(s)	85, 251, 344, 602, 887
DpnII	GATC	5 cut(s)	83, 249, 342, 600, 885
Eam1104I	CTCTTC	5 cut(s)	228, 317, 747, 826, 1013
EarI	CTCTTC	5 cut(s)	228, 317, 747, 826, 1013
Ecl136II	GAGCTC	3 cut(s)	133, 973, 1003
Eco130I	CCWWGG	2 cut(s)	126, 608
Eco24I	GRGCYC	5 cut(s)	135, 265, 363, 975, 1005
Eco31I	GGTCTC	2 cut(s)	813, 842
Eco47I	GGWCC	2 cut(s)	590, 1142
Eco47III	AGCGCT	1 cut(s)	305
Eco53kI	GAGCTC	3 cut(s)	133, 973, 1003
Eco88I	CYCGRG	2 cut(s)	258, 356
EcoICRI	GAGCTC	3 cut(s)	133, 973, 1003
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRI	GAATTC	1 cut(s)	413
EcoRII	CCWGG	3 cut(s)	27, 266, 731
EcoT14I	CCWWGG	2 cut(s)	126, 608
EcoT38I	GRGCYC	5 cut(s)	135, 265, 363, 975, 1005
ErhI	CCWWGG	2 cut(s)	126, 608
FalI	AAGNNNNNCTT	6 cut(s)	275, 307, 293, 325, 577, 609
FaqI	GGGAC	2 cut(s)	370, 520
FauI	CCCGC	5 cut(s)	386, 466, 806, 951, 998
FauNDI	CATATG	1 cut(s)	1014
FbaI	TGATCA	1 cut(s)	600
Fnu4HI	GCNGC	5 cut(s)	97, 100, 140, 1165, 1257
FokI	GGATG	4 cut(s)	209, 461, 476, 1092
FriOI	GRGCYC	5 cut(s)	135, 265, 363, 975, 1005
Fsp4HI	GCNGC	5 cut(s)	97, 100, 140, 1165, 1257
FspBI	CTAG	3 cut(s)	44, 518, 1076
FspI	TGCGCA	1 cut(s)	847
GlaI	GCGC	2 cut(s)	305, 847
GluI	GCNGC	5 cut(s)	97, 100, 140, 1165, 1257
GsaI	CCCAGC	1 cut(s)	1106
GsuI	CTGGAG	1 cut(s)	48
HaeII	RGCGCY	1 cut(s)	307
HaeIII	GGCC	1 cut(s)	257
HapII	CCGG	3 cut(s)	259, 657, 1055
HhaI	GCGC	2 cut(s)	306, 848
Hin6I	GCGC	2 cut(s)	304, 846
HinP1I	GCGC	2 cut(s)	304, 846
HindIII	AAGCTT	1 cut(s)	924
HinfI	GANTC	4 cut(s)	20, 113, 491, 508
HpaII	CCGG	3 cut(s)	259, 657, 1055
HphI	GGTGA	4 cut(s)	44, 307, 494, 1069
Hpy166II	GTNNAC	3 cut(s)	298, 353, 964
Hpy188I	TCNGA	1 cut(s)	274
Hpy188III	TCNNGA	4 cut(s)	65, 346, 917, 1205
Hpy8I	GTNNAC	3 cut(s)	298, 353, 964
HpyAV	CCTTC	4 cut(s)	187, 603, 759, 1155
HpyCH4III	ACNGT	1 cut(s)	1051
HpyCH4V	TGCA	6 cut(s)	139, 200, 438, 503, 1012, 1192
HpyF10VI	GCNNNNNNNGC	4 cut(s)	96, 139, 367, 1009
HpyF3I	CTNAG	3 cut(s)	273, 839, 1182
HspAI	GCGC	2 cut(s)	304, 846
Ksp22I	TGATCA	1 cut(s)	600
KspI	CCGCGG	2 cut(s)	462, 481
Kzo9I	GATC	5 cut(s)	83, 249, 342, 600, 885
LmnI	GCTCC	4 cut(s)	130, 268, 450, 736
Lsp1109I	GCAGC	4 cut(s)	108, 111, 151, 1151
LweI	GCATC	2 cut(s)	187, 901
MaeI	CTAG	3 cut(s)	44, 518, 1076
MaeIII	GTNAC	3 cut(s)	209, 782, 1113
MalI	GATC	5 cut(s)	85, 251, 344, 602, 887
MboI	GATC	5 cut(s)	83, 249, 342, 600, 885
MfeI	CAATTG	1 cut(s)	1240
MhlI	GDGCHC	5 cut(s)	135, 265, 363, 975, 1005
MluCI	AATT	3 cut(s)	413, 534, 1240
MlyI	GAGTC	1 cut(s)	502
MroXI	GAANNNNTTC	1 cut(s)	287
MseI	TTAA	2 cut(s)	155, 1095
MspA1I	CMGCKG	2 cut(s)	461, 480
MspI	CCGG	3 cut(s)	259, 657, 1055
MspR9I	CCNGG	5 cut(s)	29, 259, 260, 268, 733
MunI	CAATTG	1 cut(s)	1240
MvaI	CCWGG	3 cut(s)	29, 268, 733
MvnI	CGCG	2 cut(s)	461, 480
MwoI	GCNNNNNNNGC	4 cut(s)	96, 139, 367, 1009
NciI	CCSGG	2 cut(s)	259, 260
NdeI	CATATG	1 cut(s)	1014
NdeII	GATC	5 cut(s)	83, 249, 342, 600, 885
NlaIV	GGNNCC	4 cut(s)	264, 591, 640, 738
NmeAIII	GCCGAG	1 cut(s)	730
NsbI	TGCGCA	1 cut(s)	847
NspI	RCATGY	2 cut(s)	636, 898
PciI	ACATGT	2 cut(s)	632, 894
PdmI	GAANNNNTTC	1 cut(s)	287
PfeI	GAWTC	3 cut(s)	20, 113, 491
PkrI	GCNGC	5 cut(s)	98, 101, 141, 1166, 1258
PleI	GAGTC	1 cut(s)	502
PpsI	GAGTC	1 cut(s)	502
PpuMI	RGGWCCY	1 cut(s)	590
PscI	ACATGT	2 cut(s)	632, 894
Psp124BI	GAGCTC	3 cut(s)	135, 975, 1005
Psp5II	RGGWCCY	1 cut(s)	590
Psp6I	CCWGG	3 cut(s)	27, 266, 731
PspFI	CCCAGC	1 cut(s)	1102
PspGI	CCWGG	3 cut(s)	27, 266, 731
PspN4I	GGNNCC	4 cut(s)	264, 591, 640, 738
PspPI	GGNCC	3 cut(s)	256, 590, 1142
PspPPI	RGGWCCY	1 cut(s)	590
PsrI	GAACNNNNNNTAC	2 cut(s)	269, 301
PstI	CTGCAG	1 cut(s)	440
RsaI	GTAC	3 cut(s)	172, 278, 338
RsaNI	GTAC	3 cut(s)	171, 277, 337
SacI	GAGCTC	3 cut(s)	135, 975, 1005
SacII	CCGCGG	2 cut(s)	462, 481
SaqAI	TTAA	2 cut(s)	155, 1095
SatI	GCNGC	5 cut(s)	97, 100, 140, 1165, 1257
Sau3AI	GATC	5 cut(s)	83, 249, 342, 600, 885
Sau96I	GGNCC	3 cut(s)	256, 590, 1142
SbfI	CCTGCAGG	1 cut(s)	440
ScaI	AGTACT	1 cut(s)	278
SchI	GAGTC	1 cut(s)	502
ScrFI	CCNGG	5 cut(s)	29, 259, 260, 268, 733
SdaI	CCTGCAGG	1 cut(s)	440
SduI	GDGCHC	5 cut(s)	135, 265, 363, 975, 1005
SfaNI	GCATC	2 cut(s)	187, 901
SfcI	CTRYAG	1 cut(s)	436
Sfr303I	CCGCGG	2 cut(s)	462, 481
SgrBI	CCGCGG	2 cut(s)	462, 481
SinI	GGWCC	2 cut(s)	590, 1142
SmaI	CCCGGG	1 cut(s)	260
SmlI	CTYRAG	2 cut(s)	915, 1004
SmoI	CTYRAG	2 cut(s)	915, 1004
SrfI	GCCCGGGC	1 cut(s)	260
Sse8387I	CCTGCAGG	1 cut(s)	440
Sse9I	AATT	3 cut(s)	413, 534, 1240
SspMI	CTAG	3 cut(s)	44, 518, 1076
SstI	GAGCTC	3 cut(s)	135, 975, 1005
StyD4I	CCNGG	5 cut(s)	27, 257, 258, 266, 731
StyI	CCWWGG	2 cut(s)	126, 608
TaaI	ACNGT	1 cut(s)	1051
TaqI	TCGA	2 cut(s)	417, 984
TasI	AATT	3 cut(s)	413, 534, 1240
TatI	WGTACW	2 cut(s)	276, 336
TfiI	GAWTC	3 cut(s)	20, 113, 491
Tru1I	TTAA	2 cut(s)	155, 1095
Tru9I	TTAA	2 cut(s)	155, 1095
TscAI	CASTG	2 cut(s)	171, 1199
TseI	GCWGC	5 cut(s)	96, 99, 139, 1164, 1256
TspDTI	ATGAA	5 cut(s)	105, 463, 480, 1031, 1222
TspMI	CCCGGG	1 cut(s)	258
TspRI	CASTG	2 cut(s)	171, 1199
VpaK11BI	GGWCC	2 cut(s)	590, 1142
XapI	RAATTY	1 cut(s)	413
XceI	RCATGY	2 cut(s)	636, 898
XmaI	CCCGGG	1 cut(s)	258
XmnI	GAANNNNTTC	1 cut(s)	287
XspI	CTAG	3 cut(s)	44, 518, 1076
ZrmI	AGTACT	1 cut(s)	278

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.