Rorug03G0055500

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002635
Physical Location & Seq
Forward (+)
4268475 .. 4269243
769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0055500.1

Sequence Viewer

Length: 600 bp
ATGTTATCATTTGTTGAGATATTTAGCTTCCTTATCTGTCTGGACTTTGTTCCTGAAACAGGGAATATGATCAAGTTTAGATCAGGCAGTTTGACACGCCTCAAATCGAACATGCCACTCAAAATGCTCATTACTAACACAAAAGTTGGGAGGAACACAAAGGCCTTAGTTGCTGGTGTTACAGAGAGGACATTAAGGCATCCAGATGCTATGGCTTCTGTGTTTAATGCAGTTGATTCTATCAGCGCGGAATTGGCTACCATCATCCAATTAGAGGCCCCTGATGAAATCTCTGTAACCGAGAAGGATGCGAAAATAGAAGAGCTGATGGAAATGAACCAAGGTTTGCTCCAATGCATGGGGGTCAGCCATGCTTCAATCGAAACCATTCTTCGAACTACACTCAAATACAAGTTGGCTTTCAAGTTGCCAGGAGCTCGTGGTGGAGGTTCTGTTCTCACACTATTGCCAACCTTGCTATCAGCAACAGTTGTTGATAAAGTAACTGCAGAACTGGAGTCGTGTGGATTCCATTGCTTAACAGCGGCCATTGGTGGACAAAGGTGTTCAAGTTTGCTTTGGTGGTTCATCCTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

199

Amino Acids

21.74

Weight (kDa)

7.64

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 358
AccII CGCG 1 cut(s) 248
AciI CCGC 2 cut(s) 248, 545
AcoI YGGCCR 1 cut(s) 546
AfiI CCNNNNNNNGG 3 cut(s) 59, 274, 358
AgsI TTSAA 3 cut(s) 378, 424, 570
AjnI CCWGG 1 cut(s) 430
AloI GAACNNNNNNTCC 2 cut(s) 438, 470
AluBI AGCT 3 cut(s) 27, 325, 437
AluI AGCT 3 cut(s) 27, 325, 437
Alw21I GWGCWC 1 cut(s) 439
AoxI GGCC 3 cut(s) 162, 276, 546
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 277
AsuII TTCGAA 1 cut(s) 394
AxyI CCTNAGG 1 cut(s) 593
BanII GRGCYC 1 cut(s) 439
BauI CACGAG 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 439
BccI CCATC 2 cut(s) 269, 322
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 507
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 3 cut(s) 196, 208, 298
BpmI CTGGAG 1 cut(s) 536
Bpu14I TTCGAA 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 340
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 274, 358
Bse1I ACTGG 1 cut(s) 519
Bse21I CCTNAGG 1 cut(s) 593
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 340
BseGI GGATG 4 cut(s) 199, 264, 313, 588
BseLI CCNNNNNNNGG 3 cut(s) 59, 274, 358
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 3 cut(s) 164, 278, 548
BsiHKAI GWGCWC 1 cut(s) 439
BslI CCNNNNNNNGG 3 cut(s) 59, 274, 358
BsnI GGCC 3 cut(s) 164, 278, 548
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 2 cut(s) 69, 80
BspACI CCGC 2 cut(s) 248, 545
BspANI GGCC 3 cut(s) 164, 278, 548
BspCNI CTCAG 1 cut(s) 585
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 511
BspQI GCTCTTC 1 cut(s) 315
BspT104I TTCGAA 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 532
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 2 cut(s) 69, 80
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 1 cut(s) 340
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 166, 593
BstENI CCTNNNNNAGG 1 cut(s) 57
BstF5I GGATG 4 cut(s) 199, 264, 313, 588
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 2 cut(s) 72, 83
BstMBI GATC 2 cut(s) 69, 80
BstMWI GCNNNNNNNGC 3 cut(s) 170, 254, 475
BstNI CCWGG 1 cut(s) 432
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 507
BstUI CGCG 1 cut(s) 248
Bsu36I CCTNAGG 1 cut(s) 593
BsuRI GGCC 3 cut(s) 164, 278, 548
BtsCI GGATG 4 cut(s) 199, 264, 313, 588
CfoI GCGC 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 277
CviAII CATG 3 cut(s) 112, 358, 371
DdeI CTNAG 2 cut(s) 166, 593
DpnI GATC 2 cut(s) 71, 82
DpnII GATC 2 cut(s) 69, 80
EaeI YGGCCR 1 cut(s) 546
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Ecl136II GAGCTC 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 340
Eco147I AGGCCT 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 437
Eco81I CCTNAGG 1 cut(s) 593
EcoICRI GAGCTC 1 cut(s) 437
EcoNI CCTNNNNNAGG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 359
EcoT38I GRGCYC 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 3 cut(s) 115, 361, 374
FaiI YATR 5 cut(s) 68, 113, 212, 359, 372
FatI CATG 3 cut(s) 111, 357, 370
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 4 cut(s) 186, 251, 320, 575
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 1 cut(s) 546
GlaI GCGC 1 cut(s) 247
GluI GCNGC 1 cut(s) 546
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 3 cut(s) 164, 278, 548
HhaI GCGC 1 cut(s) 248
Hin1II CATG 3 cut(s) 115, 361, 374
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HinfI GANTC 3 cut(s) 236, 518, 528
Hpy166II GTNNAC 1 cut(s) 557
Hpy188III TCNNGA 4 cut(s) 41, 53, 203, 592
Hpy8I GTNNAC 1 cut(s) 557
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 3 cut(s) 230, 357, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 254, 475
HpyF3I CTNAG 2 cut(s) 166, 593
Hsp92II CATG 3 cut(s) 115, 361, 374
HspAI GCGC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 2 cut(s) 69, 80
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 354, 434
LweI GCATC 3 cut(s) 196, 208, 298
MaeIII GTNAC 3 cut(s) 178, 295, 502
MalI GATC 2 cut(s) 71, 82
MboI GATC 2 cut(s) 69, 80
MboII GAAGA 2 cut(s) 332, 383
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 2 cut(s) 251, 269
MlyI GAGTC 1 cut(s) 527
MnlI CCTC 6 cut(s) 110, 144, 180, 268, 440, 588
Mph1103I ATGCAT 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 387
MseI TTAA 3 cut(s) 194, 225, 539
MslI CAYNNNNRTG 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 3 cut(s) 170, 254, 475
NdeII GATC 2 cut(s) 69, 80
NlaIII CATG 3 cut(s) 115, 361, 374
NlaIV GGNNCC 1 cut(s) 279
NsiI ATGCAT 1 cut(s) 359
NspI RCATGY 1 cut(s) 115
NspV TTCGAA 1 cut(s) 394
PceI AGGCCT 1 cut(s) 164
PciSI GCTCTTC 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 2 cut(s) 236, 528
PflMI CCANNNNNTGG 1 cut(s) 358
PkrI GCNGC 1 cut(s) 547
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Psp124BI GAGCTC 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 277
PstI CTGCAG 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 1 cut(s) 439
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 3 cut(s) 194, 225, 539
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 2 cut(s) 69, 80
Sau96I GGNCC 1 cut(s) 277
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 1 cut(s) 439
SetI ASST 8 cut(s) 29, 327, 346, 439, 451, 476, 566, 599
SfaNI GCATC 3 cut(s) 196, 208, 298
SfcI CTRYAG 1 cut(s) 507
SfuI TTCGAA 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 204
Sse9I AATT 2 cut(s) 251, 269
SseBI AGGCCT 1 cut(s) 164
SsiI CCGC 2 cut(s) 248, 545
SstI GAGCTC 1 cut(s) 439
StuI AGGCCT 1 cut(s) 164
StyD4I CCNGG 1 cut(s) 430
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 3 cut(s) 107, 381, 394
TasI AATT 2 cut(s) 251, 269
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 2 cut(s) 236, 528
Tru1I TTAA 3 cut(s) 194, 225, 539
Tru9I TTAA 3 cut(s) 194, 225, 539
TspDTI ATGAA 3 cut(s) 300, 350, 577
Van91I CCANNNNNTGG 1 cut(s) 358
XagI CCTNNNNNAGG 1 cut(s) 57
XceI RCATGY 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 387
Zsp2I ATGCAT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.