Rorug03G0092900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005102
Physical Location & Seq
Reverse (-)
7237345 .. 7238614
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0092900.1

Sequence Viewer

Length: 639 bp
ATGGAGATTGGGATTCGATCGGTGCAGAGAGAAGAGGAGATAGTTCCTATGAAGCATATGAAGATGGACTGGATCCCCTACATTCCTCTGGAGCTCAGAGAAGGCTGGAATTCGGAAAGAGCGACCAAGTCTGGTTCGAATTTGCCTCAGATTTTCGTGCTGGGATGTACTCAGAGGAAGGCAAGCTTGAAGCAGATGAAGGAGGACCGCTGGATGAAGTTCCAGTACTGTGTGCCTTATATATTGAATCCGAAATACGAAGAAGAGAAGGAGGAGAATACTGTGGTGGAATTTTTCTTCCCACGGGAGCCTCAACTGCCCCTGGGGTGTGAATTCAATTGGGACATGGATGAAGTTGAGGATTTCACTGATAAACTTATAGAGGATGAGGAGTTCAGTGAGGATAAGAAGAAGGCATTCATGGAGTTTTTGAAGGAGGAAGTAAAGAACGCTAAGAAGAAGAAGAGGGAAGCGAGGAAGAAGAGAATGGAGGACCCAAAGTATCAGGAAGCTAAAACCTATTATGAGAATATGAGGCTTTACAAGATTTACCCAGTACAAACACCAAACACTCCTGTGATAGAGATGAAGTCTCATTATGTCAACTTCTACTACAGGTTTGCCCATGAGATTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

25.88

Weight (kDa)

5.85

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 208
AclWI GGATC 2 cut(s) 67, 80
AcsI RAATTY 4 cut(s) 109, 139, 290, 332
AfaI GTAC 3 cut(s) 169, 227, 558
AgsI TTSAA 4 cut(s) 190, 247, 337, 433
AjnI CCWGG 1 cut(s) 321
AleI CACNNNNGTG 1 cut(s) 575
AloI GAACNNNNNNTCC 2 cut(s) 377, 409
AluBI AGCT 3 cut(s) 94, 186, 512
AluI AGCT 3 cut(s) 94, 186, 512
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 2 cut(s) 67, 80
ApoI RAATTY 4 cut(s) 109, 139, 290, 332
Asp700I GAANNNNTTC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 205, 493
AsuII TTCGAA 1 cut(s) 137
AvaII GGWCC 2 cut(s) 205, 493
BamHI GGATCC 1 cut(s) 72
BanII GRGCYC 1 cut(s) 96
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 1 cut(s) 58
BciT130I CCWGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 613
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 2 cut(s) 205, 493
BmgT120I GGNCC 2 cut(s) 205, 493
BmiI GGNNCC 3 cut(s) 74, 309, 495
BmrFI CCNGG 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 548
BmuI ACTGGG 1 cut(s) 548
BpmI CTGGAG 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 137
BsaJI CCNNGG 3 cut(s) 302, 321, 322
Bse1I ACTGG 3 cut(s) 74, 223, 554
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 3 cut(s) 302, 321, 322
BseGI GGATG 4 cut(s) 170, 219, 355, 391
BseMII CTCAG 3 cut(s) 109, 161, 185
BseNI ACTGG 3 cut(s) 74, 223, 554
BseRI GAGGAG 3 cut(s) 50, 287, 404
BseYI CCCAGC 1 cut(s) 160
BsgI GTGCAG 1 cut(s) 44
Bsh1285I CGRYCG 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 597
BsmFI GGGAC 1 cut(s) 356
BsmI GAATGC 1 cut(s) 416
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 17, 72
BspACI CCGC 1 cut(s) 208
BspCNI CTCAG 3 cut(s) 108, 160, 184
BspLI GGNNCC 3 cut(s) 74, 309, 495
BspPI GGATC 2 cut(s) 67, 80
BspT104I TTCGAA 1 cut(s) 137
BsrI ACTGG 3 cut(s) 74, 223, 554
BssECI CCNNGG 3 cut(s) 302, 321, 322
BssMI GATC 2 cut(s) 17, 72
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 230, 283
Bst6I CTCTTC 4 cut(s) 27, 258, 458, 476
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 4 cut(s) 95, 147, 171, 453
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 4 cut(s) 170, 219, 355, 391
BstKTI GATC 2 cut(s) 20, 75
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 2 cut(s) 17, 72
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNI CCWGG 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 613
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 4 cut(s) 170, 219, 355, 391
BtsIMutI CAGTG 2 cut(s) 366, 403
Cac8I GCNNGC 1 cut(s) 184
Cfr13I GGNCC 2 cut(s) 205, 493
Csp6I GTAC 3 cut(s) 168, 226, 557
CviAII CATG 3 cut(s) 346, 421, 626
CviJI RGCY 6 cut(s) 94, 105, 186, 310, 512, 538
CviKI_1 RGCY 6 cut(s) 94, 105, 186, 310, 512, 538
CviQI GTAC 3 cut(s) 168, 226, 557
DdeI CTNAG 4 cut(s) 95, 147, 171, 453
DpnI GATC 2 cut(s) 19, 74
DpnII GATC 2 cut(s) 17, 72
Eam1104I CTCTTC 4 cut(s) 27, 258, 458, 476
EarI CTCTTC 4 cut(s) 27, 258, 458, 476
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 2 cut(s) 205, 493
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 493
EcoRI GAATTC 2 cut(s) 109, 332
EcoRII CCWGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 3 cut(s) 349, 424, 629
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 1 cut(s) 356
FatI CATG 3 cut(s) 345, 420, 625
FauNDI CATATG 1 cut(s) 57
FokI GGATG 4 cut(s) 177, 226, 362, 398
FriOI GRGCYC 1 cut(s) 96
GsaI CCCAGC 1 cut(s) 164
GsuI CTGGAG 1 cut(s) 110
Hin1II CATG 3 cut(s) 349, 424, 629
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 184
HinfI GANTC 2 cut(s) 13, 247
Hpy166II GTNNAC 1 cut(s) 604
Hpy188I TCNGA 5 cut(s) 98, 115, 150, 174, 252
Hpy188III TCNNGA 2 cut(s) 89, 506
Hpy8I GTNNAC 1 cut(s) 604
HpyAV CCTTC 6 cut(s) 95, 172, 193, 262, 406, 427
HpyCH4III ACNGT 2 cut(s) 230, 283
HpyCH4V TGCA 1 cut(s) 25
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 4 cut(s) 95, 147, 171, 453
Hsp92II CATG 3 cut(s) 349, 424, 629
Kzo9I GATC 2 cut(s) 17, 72
LmnI GCTCC 2 cut(s) 91, 307
MalI GATC 2 cut(s) 19, 74
MboI GATC 2 cut(s) 17, 72
MfeI CAATTG 1 cut(s) 337
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 5 cut(s) 109, 139, 290, 332, 337
MroXI GAANNNNTTC 1 cut(s) 416
MslI CAYNNNNRTG 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 323
MunI CAATTG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 416
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 1 cut(s) 316
NdeI CATATG 1 cut(s) 57
NdeII GATC 2 cut(s) 17, 72
NlaIII CATG 3 cut(s) 349, 424, 629
NlaIV GGNNCC 3 cut(s) 74, 309, 495
NspV TTCGAA 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 575
PasI CCCWGGG 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 2 cut(s) 13, 247
PflFI GACNNNGTC 1 cut(s) 127
Ple19I CGATCG 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 493
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 321
PspFI CCCAGC 1 cut(s) 160
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 3 cut(s) 74, 309, 495
PspPI GGNCC 2 cut(s) 205, 493
PspPPI RGGWCCY 1 cut(s) 493
PsuI RGATCY 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 127
PvuI CGATCG 1 cut(s) 20
RsaI GTAC 3 cut(s) 169, 227, 558
RsaNI GTAC 3 cut(s) 168, 226, 557
RseI CAYNNNNRTG 1 cut(s) 575
SacI GAGCTC 1 cut(s) 96
Sau3AI GATC 2 cut(s) 17, 72
Sau96I GGNCC 2 cut(s) 205, 493
ScaI AGTACT 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 96
SetI ASST 5 cut(s) 96, 188, 514, 521, 620
SfcI CTRYAG 1 cut(s) 613
SfuI TTCGAA 1 cut(s) 137
SinI GGWCC 2 cut(s) 205, 493
SmiMI CAYNNNNRTG 1 cut(s) 575
Sse9I AATT 5 cut(s) 109, 139, 290, 332, 337
SsiI CCGC 1 cut(s) 208
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 321
TaaI ACNGT 2 cut(s) 230, 283
TaqI TCGA 2 cut(s) 16, 137
TasI AATT 5 cut(s) 109, 139, 290, 332, 337
TatI WGTACW 3 cut(s) 167, 225, 556
TfiI GAWTC 2 cut(s) 13, 247
TscAI CASTG 2 cut(s) 373, 403
TspDTI ATGAA 7 cut(s) 65, 74, 212, 230, 366, 409, 602
TspRI CASTG 2 cut(s) 373, 403
Tth111I GACNNNGTC 1 cut(s) 127
VpaK11BI GGWCC 2 cut(s) 205, 493
XapI RAATTY 4 cut(s) 109, 139, 290, 332
XmnI GAANNNNTTC 1 cut(s) 416
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.