Rorug03G0231600

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008109
Physical Location & Seq
Reverse (-)
21364332 .. 21395371
31040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0231600.1

Sequence Viewer

Length: 5778 bp
ATGGCACCGTCGTCCAAGAAACCTCAGGGAGGGAATAACAAGAACCCCAGAGACGCTCCAAAGTTCAACAAATCCTCTAAAAAACCTTTCAAACCAAACAAGGATCACAACAACGCCGCCATGGCTTTGCAGCTCGAAGACGACGTCCCTGACTTCCCCAGAGGTGGAGGGAGCTCATTGAATCGAAAAGAACGCGACGAAATTCGGGCCGAAGTTGACGCCGAATTCGAGGCAGAGGAGAGGGAGATGAAGAAGAAAGGGAAGAAGAAAAGGGTGCCGAAGGAGAGCCTTGTAAACGAGGACGAGATGGGCTCGCTTTTCGGCGATGGCATCACTGGAAAACTACCTAGATATGCAAACAAGATCACTTTGAAGAATATATCTCCTGGGATGAAGGTTTGGGGAGTTGTTGCCGAAGTCAATGAAAAGGACCTTGTAATTAGTCTTCCAGGGGGTTTGCGTGGACTAGTTCGAGCGTCTGATGCTTTTGATCCTATTCTGGATGATGAAACTGAGGCTTTAGCAGATAGTATCCTCCCTAGCATATTCCGGATAGGGCAGCTGGTATCTTGCATTGTGTTGCAATTGGATGAGGATAAGAAAGAGAAAGGGAAACGGAAGATCTGGCTTTCTTTGCGCCTCTCTTTGTTGCATAAAGGCTTCACTTTGGATTCTGTTCAAGAAGGCATGGTCCTTACAGCCTATATTAAAAGCATCGAAGACCATGGTTACATTCTTCATTTTGGTTTATCTTCATTCACAGGGTTTTTGCCAAAAAATAGCCAAGCTGACAGCATGGTTCAAGTGAGCAGTGGTCAACTGCTTCAAGCTGCTGTTAGAAGAGTTGATAAAACCCGCAAGGTTATTCATATGAGTTCTGACCCAGAGATGGTGTCGAAGTGTGTGACCAAAGATCTCAAGGGAATTTCAATTGATCTACTTGTTCCGGGGATGATGGTCAATGCTCGTGTGCTATCCACCCTTGAAAATGGGGTTATGTTAGCATTTCTTACTTATTTTACTGGAACTGTTGATATATTTCATCTGCAAAATATTTACCCTACTACAAACTGGAAGGAAGATTACAATCAAAACAAGAAGCTCAATGCTCGAGTATTATTTGTCGATCCTTCAACTAGAGCTGTTGGTTTGACACTAAATCCACATCTTGTTCGCAACAAGGCTCCTCCTTCACATGTCAAAATTGGAGATATATGTGACGATGCAAAGGTAGTGAGGGTTGATAGAGGATTGGGCCTTCTGCTTGAAATACCATCTACTCAAGTTTCAACTCCGGCATACGTTAGTATATCTGATGTGGCTGAAGAAGTTCGGAAGCTTGAAAAGAAGTTCAAGGAAGGTACTCGTGTTCGTGTCAGAATTCTTGGGTTTAGGCACTTGGAAGGGCTTGCCACAGGGATTTTGAAGGCAAGTGCATTTGAAGGGTCAGTTTTCACTCACTCTGATGTCAAACCTGGGATGGTTGTGAGAGGTAAAATAATAGCTGTTGACAGCTTTGGGGCTATCGTGCAATTCCCTGGTGGTGTGAAGGCTCTTTGTCCTCTTACTCATATGTCTGAATTTGAAATTGCAAAGCCCAGAAAAAAATTTAAGGTTGGGGCTGAACTTCTGTTCCGTGTGCTTGGTTGCAAGTCCAAGAGAATAACTGTTACACACAAGAAAACACTTGTAAAGTCAAAACTGGGGATTCTTAGTTCATATGCTGATGCTGCTGATGGATTAATAACACATGGGTGGATAAGAAAGATTGAAGAGCGTGGCTGCTTTGTCCATTTTTACAATGGAGTCCAAGGATTTGCCCCAAGATCTGAACTCGGATTGGAGCCAGGATCTGACCCAAGTTCAATGTATCATGTTGGACAAGTTATCAAATGTAGAGTTATCGGCTCTAATGCTACATCAAGACGAATCAAACTTAGCTTGATAATACGGCCTCCAAGGGTTTCTGAGGATGATATGGCGAAGTTGGGCAGCCTGGTTTCAGGAGTTGTTGACCGGGTAACCCCCAATGCTGTCATGGTTTATGTTAATGCAAAAGGTTACTCAATGGGCACAATTTTTACAGACCACTTGGCAGATCACCATGGGCTGGCTGCTTTGATGAAGTCAGTATTGAAGCCTGGTTATGAATTTGATCAATTATTGGTTTTAGACACTGAAGGCAACAATTTGATCCTATCTGCAAAACATTCTCTGATTAATTCAGCTCCCATGCTTCCTTCAGAAGTCAGTCAAGTTCATCCTAATACGGTAGTACATGGCTACATTTGTAACTTAATTGACACTGGGTGCTTTGTTCGCTTTCTTGGGCGTGTGACTGGTTTCTCCCCAAGGCACAAGGCAATGGATGACCATAAAGGGGATCTTTCAGAAGCCTATTACAGTGGACAGTCTGTTCGGAGTACTATACTCGATGTCAATAGTGAAACAGGTAGGATAACACTATCATTGAAGCAGTCATCTTGCTCTTCAACAGATGCCTCCTTTATCCGGGAATACTTTATTTCAGAGGAGAAGATTGCAAAGCTGCAACTGTTGGATTCAAAAGAATCCAAATCAGATTGGTCAGAAGGATTTTCCATTGGCAGTGTTGTTGAAGGGAAGGTCCAGGAGGCAAAGGACATTGGAGTTGTTGTCAGCTTTGAGAAATATAATGATGTTTTCGGTTTCATCACGCACTATCAATTAGCTGGTACCACTGTAGAAACAGGCTCCATTGTTCGAGCAGTTGTACTTGATGTTGCCAAGGCAGAGCACCTTGTTGACTTATCGCTCAAACCAGAATTCATTACTAACTTAAAACAAGAAAGCTCTAAGAGCCAAACTCATATGAAGAAACGAAGAAGAGAAATCTCGGATGATCCGGAGATGCATGAGACCGTAAATGCTGTGGTTGAGATTGTGAAGGAGAATTACCTGGTTCTTTCTATCCCAAAGTATAACTATGCTGTAGGATATGCATCAATTTCTGACTACAACACTCAAAAGTTTCCTCAGAAACAGTTTCTGAATGGACAAAGTGTCAATGCTACTGTTATGGCCCTTCCAAGCCCTACAACCGTTGGGAGATTGCTTTTGCTACTTAATTCTTTGAGTGAGTCCGCTGATACTTCCAGTTCAAAGCGGGCAAAGAAGAATTCCAGCTACAAAGTGGGATCTGTGGTCCAGGCAGAGATAACTGAAATAAAGCCTCTTGAACTAAGATTGAAGTTTGGAATCAGCTTTCATGGACGGGTCCGTATAACAGAGGTAAATGATGATCTTTTGGAAGATCCATTTAACAACTTCAGAATTGGGCAAACAGTCACTGCAAGGATTGTTGCAAAGACTAATTATTCAGATAACAACAAAAAGAGTTTCCAGTGGGATCTGTCATTGAAACCTAGTTTGTTAACAGGTTCCTGTGAAATAGAGGAAAATATTTTGAAAGGAGATCTCGATTTCTCAATTGGAAAACATGTCACTGGCTATGTGTACAAGGTGGATGCTGAATGGGTCTGGTTAACAATATCTCGAAATGTTAGGGCTCAGCTTTTCATTCTTGACAGCGCTTGTGAGCCTAGTGAACTTCAAGAGTTTCAGAAACGCTTTCATGTGGGAAATGCTGTATCTGGTCATGTTTTAAGTGTTAGCAAGGAGAAAAAGCTATTACGGCTGGTTTCATATCCTTTTACTCCCGTCGCTGATAAAGCTGTTGATAATGAGGTGTCAAAGACGGATGCAAATGTTAGCATGTTGAATGCAACTGCTCATATTCGCGAGAGATGTGTTGTAGGTGGGAGAATTATTAAAAAACTTCCAGGTGTTGGTGGATTGACTGTGCAAATTGGTCCCCATATGTATGGCAGGGTTCATTATAGTGAGCTTTCTGACTCATGGGTGTCCAACCCATTGTCTGGATATGAGGAAGGTCAATTTGTCAAATGCAAGGTCGTTGAATGTAGTCGCTCTGGACAGGGAACTTTTCACATTGACTTGTCATTACGGTCTTCTTTAGTTGGCACACTTTGTCAAGATTCCAATGAACCTGACAATGACAAGCATACTCATACCGAACGGCCGGAAAAGATTGACAATCTGAAACCTAATATGGTCATACAGGGTTATGTGAAAAATGTTTCATCAAAAGGATGTTTTATTTTGCTTTCGCGGAAGCTTGATGCCAAAATTCTCGTCTCCAATTTATCCGATGGATATGTTGATGATCCTGAAAAGGAATTCCCAGTTGGAAAACTTGTTACTGGCAGGGTGTCATCTGTGGAGCCTTTATCAAAGAGAGTTGAAGTTACCTTGAAGTCACTGAGTGCAAGCAGTTTGCCACAATCTGCAAGGAATAATTTGGATAGCTTGCATGTCGGAGATATTATCTCTGGCAGGGTAAAACGAGTTGAGTCATATGGATTATTCATCACAATTGACAATACAAATGTGGTTGGATTATGCCACATGTCAGAGCTTGCAGAAGATAAGAAGGACAATTTTGAAACTAAATACAAAACAGGAGAGAGGGTATCAGCAAAAGTATTGAAGGTGGACAAGGAGAGGCACCGAGTTTCTCTCGGAATGAAGGATTTGTATATTATGGATAGCGACCACACACCTCCAAAACAAGATCTTGATGAACCTATCAGACAGACTGATTTGGTGGATGATTCTAAGTCAGTTATGTGTCCTGTTGGCAGTTTCCTTGGGGACCAGAACATGGATATTGAACATGAAAATGCAGAGTTCCAGTTTCTTGCACAAGCAGAATCAAGAGCTTTAGTTCCTCCACTTGAAGTCACTCTTGATGATGTAGACCAATGCGATGATATTGTTAGCCAGGATCAAGAGCTTCCTGATGCGGAGGACGCTGTAGATGACAAGAAAAAGAAGCTCACGAAGAAAAAAGCCAGGGATGAGAGGGAGCGTGAGATAAGGGCTGCTGAGGAAAGATTATTGGAGAAAGATATACCTAGGACTGATGAGGAATTTGAGAAACTAGTTAGAAGTTCTCCAAATAACAGTTATGTTTGGATAAAATATATGGAATTTGTGCTCTCTATGGCTGATGTTGAGAAGGCACGCTCTATTGCTAAAAGGGCTTTGGAGACGATAAACTTTCGAGAAGAGAACGAGAAGCTCAATGTTTGGGTGGCTTATTTTAATTTGGAAAGCAAATATGGAAGTCCTCCAGAGGAGGCTGTAATGAAAGTCTTCCAAAAAGCGTTGCAGTATAATGATCCCAAAAAGGTTCATCTAGCACTCTTGGGAGTCTTTGAGAGGTCTGAGCAACACAAGTTGGCTGATGAACTTGCTGATAATATGATTAAGAGGTTTAAGAAGTCTTGCAAGGTTTGGCTAAGGCGGGTACAGAGGTTCTTGGTGCAACAGCAGGATGGGGTTCAGGATTTCGTCTCTCGTGCTGAAAAGATCCTTCCTAAGCATAAGCATATCAAATTCTTGTCACAGACAGCCATCCTGGAGTTCAAATGTGGGAACCCAGAGAGAGGGCGCTCCATGTTTGAAAATATTTTGAGGCAAAACCCGAAGAGAACAGACTTGTGGAGTGTTTATCTTGATCAAGAAATTCGACTTGGAGATACTGATCTGATTCATGCATTGTTTGAGAGGGCAACTAGTTTGAGCCTACCAGCTAAAAAGATGAAATTCTTGTTCAAAAAATATCTGGAATATGAGGAGCGTCACGGTGACAAAGAGAGGGCCAATTACGTGAAACAGAAGGCAATGAGTTATGTTGAGAATACAGTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

1925

Amino Acids

215.37

Weight (kDa)

8.65

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1_RRP5 PF23459 128 - 201 6.3e-06 RRP5 S1 domain
S1_RRP5 PF23459 315 - 386 4e-06 RRP5 S1 domain
OB_RRP5_4th PF24685 395 - 485 4.2e-40 RRP5 OB-fold domain
S1 PF00575 490 - 554 2.4e-08 S1 RNA binding domain
S1 PF00575 584 - 648 4.1e-06 S1 RNA binding domain
S1 PF00575 755 - 825 1.7e-06 S1 RNA binding domain
OB_RRP5 PF24682 950 - 1037 1.1e-38 RRP5 OB-fold domain
S1_RRP5 PF23459 1367 - 1437 5.1e-07 RRP5 S1 domain
S1 PF00575 1459 - 1530 4.1e-16 S1 RNA binding domain
HAT_Syf1_CNRKL1_C PF23231 1673 - 1922 4.5e-10 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
Suf PF05843 1823 - 1921 1.5e-06 Suppressor of forked protein (Suf)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
Acc65I GGTACC 1 cut(s) 2714
AccB1I GGYRCC 4 cut(s) 4, 274, 2714, 4566
AccB7I CCANNNNNTGG 4 cut(s) 2110, 3827, 3917, 4723
AccI GTMKAC 1 cut(s) 4818
AccII CGCG 3 cut(s) 195, 3780, 4171
AccIII TCCGGA 2 cut(s) 549, 2884
AciI CCGC 7 cut(s) 117, 856, 3123, 3145, 4171, 4865, 5368
AcoI YGGCCR 1 cut(s) 4079
AcuI CTGAAG 4 cut(s) 1344, 2199, 2226, 3292
AcyI GRCGYC 2 cut(s) 144, 219
AdeI CACNNNGTG 2 cut(s) 2310, 4325
AfaI GTAC 7 cut(s) 1363, 2277, 2425, 2716, 2754, 3497, 5373
AfeI AGCGCT 1 cut(s) 3571
AflIII ACRYGT 3 cut(s) 1195, 3478, 4467
AhdI GACNNNNNGTC 1 cut(s) 3041
AhlI ACTAGT 3 cut(s) 466, 5002, 5639
AjuI GAANNNNNNNTTGG 4 cut(s) 4230, 4262, 5580, 5612
AleI CACNNNNGTG 1 cut(s) 1753
AloI GAACNNNNNNTCC 6 cut(s) 1617, 1649, 1699, 1731, 2400, 2432
Alw21I GWGCWC 3 cut(s) 176, 2778, 5061
Alw26I GTCTC 5 cut(s) 45, 2891, 4201, 5105, 5422
AlwNI CAGNNNCTG 3 cut(s) 1853, 3028, 3329
Ama87I CYCGRG 1 cut(s) 1110
Aor13HI TCCGGA 2 cut(s) 549, 2884
Aor51HI AGCGCT 1 cut(s) 3571
AoxI GGCC 6 cut(s) 207, 1255, 1952, 3060, 4079, 5724
ApeKI GCWGC 9 cut(s) 130, 559, 830, 1730, 1782, 1992, 2114, 2548, 4943
AseI ATTAAT 2 cut(s) 1742, 2220
Asp700I GAANNNNTTC 2 cut(s) 1329, 5216
Asp718I GGTACC 1 cut(s) 2714
AspA2I CCTAGG 1 cut(s) 4976
AspLEI GCGC 3 cut(s) 639, 3572, 5517
AsuC2I CCSGG 3 cut(s) 948, 2018, 2513
AsuHPI GGTGA 2 cut(s) 2093, 5723
AvaI CYCGRG 1 cut(s) 1110
AvaII GGWCC 7 cut(s) 430, 691, 2626, 3184, 3256, 3851, 4714
AvrII CCTAGG 1 cut(s) 4976
AxyI CCTNAGG 1 cut(s) 24
BaeGI GKGCMC 1 cut(s) 2075
BaeI ACNNNNGTAYC 6 cut(s) 1353, 1386, 2706, 2739, 5363, 5396
BanI GGYRCC 4 cut(s) 4, 274, 2714, 4566
BanII GRGCYC 3 cut(s) 176, 314, 3550
BarI GAAGNNNNNNTAC 6 cut(s) 429, 461, 1067, 1099, 2918, 2950
BauI CACGAG 3 cut(s) 966, 1365, 5421
BbsI GAAGAC 5 cut(s) 144, 437, 726, 4002, 5209
Bbv12I GWGCWC 3 cut(s) 176, 2778, 5061
BbvCI CCTCAGC 1 cut(s) 4947
BbvI GCAGC 9 cut(s) 142, 571, 817, 1717, 1769, 2004, 2101, 2535, 4930
BceAI ACGGC 3 cut(s) 1967, 3689, 4094
BciVI GTATCC 1 cut(s) 542
BclI TGATCA 2 cut(s) 2155, 5581
BcnI CCSGG 3 cut(s) 948, 2018, 2513
BcoDI GTCTC 5 cut(s) 45, 2891, 4201, 5105, 5422
BcuI ACTAGT 3 cut(s) 466, 5002, 5639
BfmI CTRYAG 3 cut(s) 2721, 2970, 4875
BfoI RGCGCY 2 cut(s) 3573, 5518
BfuI GTATCC 1 cut(s) 542
BglI GCCNNNNNGGC 1 cut(s) 122
BglII AGATCT 5 cut(s) 621, 913, 1826, 3454, 4633
BlnI CCTAGG 1 cut(s) 4976
BlpI GCTNAGC 1 cut(s) 3549
BmcAI AGTACT 1 cut(s) 2425
Bme18I GGWCC 7 cut(s) 430, 691, 2626, 3184, 3256, 3851, 4714
BmeRI GACNNNNNGTC 1 cut(s) 3041
BmeT110I CYCGRG 1 cut(s) 1110
BmrI ACTGGG 3 cut(s) 1712, 2316, 4238
BmuI ACTGGG 3 cut(s) 1712, 2316, 4238
BpiI GAAGAC 5 cut(s) 144, 437, 726, 4002, 5209
BplI GAGNNNNNCTC 6 cut(s) 296, 328, 2830, 2862, 4563, 4595
BpmI CTGGAG 2 cut(s) 5178, 5504
Bpu10I CCTNAGC 3 cut(s) 4947, 5363, 5442
Bpu1102I GCTNAGC 1 cut(s) 3549
BpuEI CTTGAG 2 cut(s) 902, 1266
BpuMI CCSGG 3 cut(s) 948, 2018, 2513
BsaAI YACGTR 1 cut(s) 5734
BsaBI GATNNNNATC 3 cut(s) 1086, 2980, 5607
BsaHI GRCGYC 2 cut(s) 144, 219
BsaI GGTCTC 1 cut(s) 2891
BsaWI WCCGGW 2 cut(s) 549, 2884
BsaXI ACNNNNNCTCC 4 cut(s) 2640, 2670, 4515, 4545
Bse21I CCTNAGG 1 cut(s) 24
Bse3DI GCAATG 2 cut(s) 2370, 5754
Bse8I GATNNNNATC 3 cut(s) 1086, 2980, 5607
BseAI TCCGGA 2 cut(s) 549, 2884
BseJI GATNNNNATC 3 cut(s) 1086, 2980, 5607
BseMI GCAATG 2 cut(s) 2370, 5754
BseMII CTCAG 8 cut(s) 38, 504, 1959, 3029, 3563, 4313, 4938, 5280
BseRI GAGGAG 5 cut(s) 251, 1176, 2546, 5213, 5714
BseSI GKGCMC 1 cut(s) 2075
BseX3I CGGCCG 1 cut(s) 4079
BseXI GCAGC 9 cut(s) 142, 571, 817, 1717, 1769, 2004, 2101, 2535, 4930
Bsh1236I CGCG 3 cut(s) 195, 3780, 4171
Bsh1285I CGRYCG 1 cut(s) 4082
BshFI GGCC 6 cut(s) 209, 1257, 1954, 3062, 4081, 5726
BshNI GGYRCC 4 cut(s) 4, 274, 2714, 4566
BsiEI CGRYCG 1 cut(s) 4082
BsiHKAI GWGCWC 3 cut(s) 176, 2778, 5061
BsiHKCI CYCGRG 1 cut(s) 1110
BsiSI CCGG 7 cut(s) 550, 947, 1295, 2017, 2512, 2885, 4082
BslFI GGGAC 3 cut(s) 131, 3837, 4727
BsmAI GTCTC 5 cut(s) 45, 2891, 4201, 5105, 5422
BsmBI CGTCTC 4 cut(s) 45, 4201, 5105, 5422
BsmFI GGGAC 3 cut(s) 131, 3837, 4727
BsmI GAATGC 1 cut(s) 3766
BsnI GGCC 6 cut(s) 209, 1257, 1954, 3062, 4081, 5726
Bso31I GGTCTC 1 cut(s) 2891
BsoBI CYCGRG 1 cut(s) 1110
Bsp1286I GDGCHC 6 cut(s) 176, 314, 2075, 2778, 3550, 5061
Bsp13I TCCGGA 2 cut(s) 549, 2884
Bsp1407I TGTACA 1 cut(s) 3495
Bsp1720I GCTNAGC 1 cut(s) 3549
Bsp19I CCATGG 3 cut(s) 120, 724, 2104
Bsp68I TCGCGA 1 cut(s) 3780
BspACI CCGC 7 cut(s) 117, 856, 3123, 3145, 4171, 4865, 5368
BspANI GGCC 6 cut(s) 209, 1257, 1954, 3062, 4081, 5726
BspCNI CTCAG 8 cut(s) 37, 505, 1960, 3028, 3562, 4314, 4939, 5281
BspEI TCCGGA 2 cut(s) 549, 2884
BspFNI CGCG 3 cut(s) 195, 3780, 4171
BspQI GCTCTTC 2 cut(s) 1767, 2494
BspT107I GGYRCC 4 cut(s) 4, 274, 2714, 4566
BspTNI GGTCTC 1 cut(s) 2891
BsrDI GCAATG 2 cut(s) 2370, 5754
BsrGI TGTACA 1 cut(s) 3495
BssNI GRCGYC 2 cut(s) 144, 219
BssSI CACGAG 3 cut(s) 966, 1365, 5421
BssT1I CCWWGG 9 cut(s) 120, 724, 1810, 1958, 2104, 2351, 2766, 4708, 4976
Bst2BI CACGAG 3 cut(s) 966, 1365, 5421
Bst6I CTCTTC 6 cut(s) 835, 1767, 2494, 2860, 5124, 5546
BstACI GRCGYC 2 cut(s) 144, 219
BstAUI TGTACA 1 cut(s) 3495
BstBAI YACGTR 1 cut(s) 5734
BstC8I GCNNGC 8 cut(s) 314, 1410, 2112, 3147, 4330, 4370, 4479, 5086
BstDSI CCRYGG 3 cut(s) 120, 724, 2104
BstEII GGTNACC 1 cut(s) 2020
BstENI CCTNNNNNAGG 1 cut(s) 27
BstFNI CGCG 3 cut(s) 195, 3780, 4171
BstH2I RGCGCY 2 cut(s) 3573, 5518
BstHHI GCGC 3 cut(s) 639, 3572, 5517
BstMAI GTCTC 5 cut(s) 45, 2891, 4201, 5105, 5422
BstMCI CGRYCG 1 cut(s) 4082
BstNSI RCATGY 5 cut(s) 1199, 3482, 3757, 4376, 4471
BstPI GGTNACC 1 cut(s) 2020
BstSFI CTRYAG 3 cut(s) 2721, 2970, 4875
BstSLI GKGCMC 1 cut(s) 2075
BstUI CGCG 3 cut(s) 195, 3780, 4171
BstV1I GCAGC 9 cut(s) 142, 571, 817, 1717, 1769, 2004, 2101, 2535, 4930
BstV2I GAAGAC 5 cut(s) 144, 437, 726, 4002, 5209
BstXI CCANNNNNNTGG 1 cut(s) 3863
BstZI CGGCCG 1 cut(s) 4079
Bsu36I CCTNAGG 1 cut(s) 24
BsuI GTATCC 1 cut(s) 542
BsuRI GGCC 6 cut(s) 209, 1257, 1954, 3062, 4081, 5726
BtgI CCRYGG 3 cut(s) 120, 724, 2104
BtgZI GCGATG 2 cut(s) 339, 4842
BtsI GCAGTG 3 cut(s) 817, 2614, 3327
BtuMI TCGCGA 1 cut(s) 3780
Cac8I GCNNGC 8 cut(s) 314, 1410, 2112, 3147, 4330, 4370, 4479, 5086
CaiI CAGNNNCTG 3 cut(s) 1853, 3028, 3329
CfoI GCGC 3 cut(s) 639, 3572, 5517
CseI GACGC 5 cut(s) 62, 227, 465, 4880, 5693
CsiI ACCWGGT 1 cut(s) 2937
Csp6I GTAC 7 cut(s) 1362, 2276, 2424, 2715, 2753, 3496, 5372
CviQI GTAC 7 cut(s) 1362, 2276, 2424, 2715, 2753, 3496, 5372
DraIII CACNNNGTG 2 cut(s) 2310, 4325
DriI GACNNNNNGTC 1 cut(s) 3041
EaeI YGGCCR 1 cut(s) 4079
EagI CGGCCG 1 cut(s) 4079
Eam1104I CTCTTC 6 cut(s) 835, 1767, 2494, 2860, 5124, 5546
Eam1105I GACNNNNNGTC 1 cut(s) 3041
EarI CTCTTC 6 cut(s) 835, 1767, 2494, 2860, 5124, 5546
Ecl136II GAGCTC 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 4079
Eco130I CCWWGG 9 cut(s) 120, 724, 1810, 1958, 2104, 2351, 2766, 4708, 4976
Eco24I GRGCYC 3 cut(s) 176, 314, 3550
Eco31I GGTCTC 1 cut(s) 2891
Eco47I GGWCC 7 cut(s) 430, 691, 2626, 3184, 3256, 3851, 4714
Eco47III AGCGCT 1 cut(s) 3571
Eco52I CGGCCG 1 cut(s) 4079
Eco53kI GAGCTC 1 cut(s) 174
Eco57I CTGAAG 4 cut(s) 1344, 2199, 2226, 3292
Eco81I CCTNAGG 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 1110
Eco91I GGTNACC 1 cut(s) 2020
EcoICRI GAGCTC 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 2020
EcoRI GAATTC 5 cut(s) 224, 1380, 2804, 3157, 4238
EcoT14I CCWWGG 9 cut(s) 120, 724, 1810, 1958, 2104, 2351, 2766, 4708, 4976
EcoT22I ATGCAT 3 cut(s) 2895, 2983, 5623
EcoT38I GRGCYC 3 cut(s) 176, 314, 3550
ErhI CCWWGG 9 cut(s) 120, 724, 1810, 1958, 2104, 2351, 2766, 4708, 4976
Esp3I CGTCTC 4 cut(s) 45, 4201, 5105, 5422
FalI AAGNNNNNCTT 4 cut(s) 2370, 2402, 4791, 4823
FaqI GGGAC 3 cut(s) 131, 3837, 4727
FauI CCCGC 3 cut(s) 863, 3138, 5361
FauNDI CATATG 6 cut(s) 870, 1572, 1720, 2850, 3858, 4417
FbaI TGATCA 2 cut(s) 2155, 5581
FblI GTMKAC 1 cut(s) 4818
FriOI GRGCYC 3 cut(s) 176, 314, 3550
GlaI GCGC 3 cut(s) 638, 3571, 5516
GsuI CTGGAG 2 cut(s) 5178, 5504
HaeII RGCGCY 2 cut(s) 3573, 5518
HaeIII GGCC 6 cut(s) 209, 1257, 1954, 3062, 4081, 5726
HapII CCGG 7 cut(s) 550, 947, 1295, 2017, 2512, 2885, 4082
HgaI GACGC 5 cut(s) 62, 227, 465, 4880, 5693
HhaI GCGC 3 cut(s) 639, 3572, 5517
Hin1I GRCGYC 2 cut(s) 144, 219
Hin6I GCGC 3 cut(s) 637, 3570, 5515
HinP1I GCGC 3 cut(s) 637, 3570, 5515
HincII GTYRAC 7 cut(s) 217, 818, 1510, 2014, 2785, 3414, 3525
HindII GTYRAC 7 cut(s) 217, 818, 1510, 2014, 2785, 3414, 3525
HindIII AAGCTT 2 cut(s) 1337, 4175
HpaI GTTAAC 2 cut(s) 3414, 3525
HpaII CCGG 7 cut(s) 550, 947, 1295, 2017, 2512, 2885, 4082
HphI GGTGA 2 cut(s) 2093, 5723
Hpy99I CGWCG 4 cut(s) 13, 146, 200, 3704
HpyCH4IV ACGT 3 cut(s) 144, 1302, 5733
HpySE526I ACGT 3 cut(s) 144, 1302, 5733
Hsp92I GRCGYC 2 cut(s) 144, 219
HspAI GCGC 3 cut(s) 637, 3570, 5515
Kpn2I TCCGGA 2 cut(s) 549, 2884
KpnI GGTACC 1 cut(s) 2718
Ksp22I TGATCA 2 cut(s) 2155, 5581
KspAI GTTAAC 2 cut(s) 3414, 3525
LguI GCTCTTC 2 cut(s) 1767, 2494
Lsp1109I GCAGC 9 cut(s) 142, 571, 817, 1717, 1769, 2004, 2101, 2535, 4930
MabI ACCWGGT 1 cut(s) 2937
MaeII ACGT 3 cut(s) 144, 1302, 5733
MfeI CAATTG 4 cut(s) 584, 930, 3468, 4434
MhlI GDGCHC 6 cut(s) 176, 314, 2075, 2778, 3550, 5061
MlyI GAGTC 5 cut(s) 1815, 3128, 3887, 4421, 5283
MmeI TCCRAC 6 cut(s) 1858, 2538, 3930, 4228, 4357, 4435
Mph1103I ATGCAT 3 cut(s) 2895, 2983, 5623
MroI TCCGGA 2 cut(s) 549, 2884
MroXI GAANNNNTTC 2 cut(s) 1329, 5216
MslI CAYNNNNRTG 5 cut(s) 1464, 1753, 3861, 3879, 4740
MspA1I CMGCKG 2 cut(s) 562, 3125
MspI CCGG 7 cut(s) 550, 947, 1295, 2017, 2512, 2885, 4082
MunI CAATTG 4 cut(s) 584, 930, 3468, 4434
Mva1269I GAATGC 1 cut(s) 3766
MvnI CGCG 3 cut(s) 195, 3780, 4171
NciI CCSGG 3 cut(s) 948, 2018, 2513
NcoI CCATGG 3 cut(s) 120, 724, 2104
NdeI CATATG 6 cut(s) 870, 1572, 1720, 2850, 3858, 4417
NruI TCGCGA 1 cut(s) 3780
NsiI ATGCAT 3 cut(s) 2895, 2983, 5623
NspI RCATGY 5 cut(s) 1199, 3482, 3757, 4376, 4471
OliI CACNNNNGTG 1 cut(s) 1753
PaeR7I CTCGAG 1 cut(s) 1110
PciI ACATGT 3 cut(s) 1195, 3478, 4467
PciSI GCTCTTC 2 cut(s) 1767, 2494
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 225
PctI GAATGC 1 cut(s) 3766
PdmI GAANNNNTTC 2 cut(s) 1329, 5216
PflFI GACNNNGTC 1 cut(s) 143
PflMI CCANNNNNTGG 4 cut(s) 2110, 3827, 3917, 4723
PfoI TCCNGGA 3 cut(s) 2511, 2628, 5481
PleI GAGTC 5 cut(s) 1814, 3127, 3887, 4420, 5282
PpsI GAGTC 5 cut(s) 1814, 3127, 3887, 4420, 5282
Ppu21I YACGTR 1 cut(s) 5734
PpuMI RGGWCCY 1 cut(s) 430
PscI ACATGT 3 cut(s) 1195, 3478, 4467
PshBI ATTAAT 2 cut(s) 1742, 2220
Psp124BI GAGCTC 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 430
PspEI GGTNACC 1 cut(s) 2020
PspPPI RGGWCCY 1 cut(s) 430
PspXI VCTCGAGB 1 cut(s) 1110
PstNI CAGNNNCTG 3 cut(s) 1853, 3028, 3329
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 562
RruI TCGCGA 1 cut(s) 3780
RsaI GTAC 7 cut(s) 1363, 2277, 2425, 2716, 2754, 3497, 5373
RsaNI GTAC 7 cut(s) 1362, 2276, 2424, 2715, 2753, 3496, 5372
RseI CAYNNNNRTG 5 cut(s) 1464, 1753, 3861, 3879, 4740
SacI GAGCTC 1 cut(s) 176
SapI GCTCTTC 2 cut(s) 1767, 2494
ScaI AGTACT 1 cut(s) 2425
SchI GAGTC 5 cut(s) 1815, 3128, 3887, 4421, 5283
SduI GDGCHC 6 cut(s) 176, 314, 2075, 2778, 3550, 5061
SexAI ACCWGGT 1 cut(s) 2937
SfcI CTRYAG 3 cut(s) 2721, 2970, 4875
Sfr274I CTCGAG 1 cut(s) 1110
SinI GGWCC 7 cut(s) 430, 691, 2626, 3184, 3256, 3851, 4714
SlaI CTCGAG 1 cut(s) 1110
SmiMI CAYNNNNRTG 5 cut(s) 1464, 1753, 3861, 3879, 4740
SmlI CTYRAG 3 cut(s) 917, 1110, 1281
SmoI CTYRAG 3 cut(s) 917, 1110, 1281
SpeI ACTAGT 3 cut(s) 466, 5002, 5639
SsiI CCGC 7 cut(s) 117, 856, 3123, 3145, 4171, 4865, 5368
SspI AATATT 3 cut(s) 1054, 3442, 5533
SstI GAGCTC 1 cut(s) 176
StyI CCWWGG 9 cut(s) 120, 724, 1810, 1958, 2104, 2351, 2766, 4708, 4976
TaiI ACGT 3 cut(s) 147, 1305, 5736
TatI WGTACW 4 cut(s) 2275, 2423, 2752, 3495
TauI GCSGC 1 cut(s) 119
TseI GCWGC 9 cut(s) 130, 559, 830, 1730, 1782, 1992, 2114, 2548, 4943
TspGWI ACGGA 4 cut(s) 631, 1625, 3248, 3752
Tth111I GACNNNGTC 1 cut(s) 143
Van91I CCANNNNNTGG 4 cut(s) 2110, 3827, 3917, 4723
VpaK11BI GGWCC 7 cut(s) 430, 691, 2626, 3184, 3256, 3851, 4714
VspI ATTAAT 2 cut(s) 1742, 2220
XagI CCTNNNNNAGG 1 cut(s) 27
XceI RCATGY 5 cut(s) 1199, 3482, 3757, 4376, 4471
XcmI CCANNNNNNNNNTGG 3 cut(s) 1799, 2035, 3169
XhoI CTCGAG 1 cut(s) 1110
XmaJI CCTAGG 1 cut(s) 4976
XmiI GTMKAC 1 cut(s) 4818
XmnI GAANNNNTTC 2 cut(s) 1329, 5216
ZraI GACGTC 1 cut(s) 145
ZrmI AGTACT 1 cut(s) 2425
Zsp2I ATGCAT 3 cut(s) 2895, 2983, 5623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.