Rorug03G0253000

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006077
Physical Location & Seq
Forward (+)
23985205 .. 23987006
1802 bp
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UTR
Exon/CDS
Intron
Rorug03G0253000.1

Sequence Viewer

Length: 609 bp
ATGTTGAATGACGATGAGCTGTTTTTCAACACTATGGCAGAGGATGGAAGCGATGAGGAGGTAGCTGAAAAATTAACGATTATGAATGAAGAATGTTTAGATGAAGAATATACCAATGAGTATGATGAAGAAGAAGAGGAAAACCACTATGCTTCTCGTGCCATATCCAAGATGCAACCCAGGAGGTCTAGTGTATCCAAGGCTTACTGGGATGATGAGATGGGCATGGCTGTCGTATTTCCAAAGGGTAGAATTTGGAGAACCACGGGAATCTCTCGTAATGGCAAGCTCTATATCTCCATTGAGGAAGTTTTGTATTTGGTTGAAATAGGGGCTTTGCTTCTCTTGGATGGTAGTGATACTAGTATTTCCTTGGAAGATATATATACAAAGGTTTCAGATGGAAAGAATAAGTTCTTGTTGGAGGAATTTAGAGCTTATAGGCAACTCAGATACCTTGGGTACATTGTTGCGAGACATGGTATTCCCTGGTCTAGCAAGGGGGTCAAGACTAACTGTGAATCTGTTTCTTCTCAAGTTTGTCCTGAAGCTGAAGAGATTAGGTCTGTCAATGGACTGTTCAATGGGATACAGATTAATGATACTTGA

Protein Analysis

202

Amino Acids

23.1

Weight (kDa)

4.49

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_int_end_N2 PF12928 61 - 115 1.1e-13 tRNA-splicing endonuclease subunit sen54 N-term
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 252, 428
AcuI CTGAAG 2 cut(s) 567, 573
AfaI GTAC 1 cut(s) 464
AgsI TTSAA 4 cut(s) 7, 28, 326, 583
AhlI ACTAGT 1 cut(s) 362
AjnI CCWGG 2 cut(s) 179, 488
AluBI AGCT 5 cut(s) 19, 65, 289, 437, 551
AluI AGCT 5 cut(s) 19, 65, 289, 437, 551
Alw26I GTCTC 1 cut(s) 469
ApoI RAATTY 2 cut(s) 252, 428
AseI ATTAAT 1 cut(s) 597
Asp700I GAANNNNTTC 1 cut(s) 413
BauI CACGAG 1 cut(s) 156
BccI CCATC 4 cut(s) 38, 214, 344, 395
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BciT130I CCWGG 2 cut(s) 181, 490
BciVI GTATCC 2 cut(s) 205, 582
BcoDI GTCTC 1 cut(s) 469
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 3 cut(s) 189, 363, 495
BfuI GTATCC 2 cut(s) 205, 582
Bme1390I CCNGG 2 cut(s) 181, 490
BmrFI CCNGG 2 cut(s) 181, 490
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 1 cut(s) 162
BmuI ACTGGG 1 cut(s) 217
BpuEI CTTGAG 1 cut(s) 519
BsaJI CCNNGG 6 cut(s) 179, 198, 264, 372, 457, 488
BsaXI ACNNNNNCTCC 2 cut(s) 175, 205
Bse1I ACTGG 1 cut(s) 212
BseBI CCWGG 2 cut(s) 181, 490
BseDI CCNNGG 6 cut(s) 179, 198, 264, 372, 457, 488
BseGI GGATG 3 cut(s) 49, 217, 355
BseMII CTCAG 1 cut(s) 463
BseNI ACTGG 1 cut(s) 212
BseRI GAGGAG 1 cut(s) 71
BsmAI GTCTC 1 cut(s) 469
BspCNI CTCAG 1 cut(s) 462
BsrI ACTGG 1 cut(s) 212
BssECI CCNNGG 6 cut(s) 179, 198, 264, 372, 457, 488
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 3 cut(s) 198, 372, 457
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 2 cut(s) 181, 490
Bst4CI ACNGT 2 cut(s) 518, 579
Bst6I CTCTTC 2 cut(s) 129, 549
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 1 cut(s) 449
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 49, 217, 355
BstMAI GTCTC 1 cut(s) 469
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNI CCWGG 2 cut(s) 181, 490
BstSCI CCNGG 2 cut(s) 179, 488
BsuI GTATCC 2 cut(s) 205, 582
BtgI CCRYGG 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 66
BtsCI GGATG 3 cut(s) 49, 217, 355
Cac8I GCNNGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 463
CviAII CATG 2 cut(s) 226, 479
CviJI RGCY 8 cut(s) 19, 65, 203, 230, 289, 335, 437, 551
CviKI_1 RGCY 8 cut(s) 19, 65, 203, 230, 289, 335, 437, 551
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 1 cut(s) 449
Eam1104I CTCTTC 2 cut(s) 129, 549
EarI CTCTTC 2 cut(s) 129, 549
Eco130I CCWWGG 3 cut(s) 198, 372, 457
Eco57I CTGAAG 2 cut(s) 567, 573
EcoRII CCWGG 2 cut(s) 179, 488
EcoT14I CCWWGG 3 cut(s) 198, 372, 457
ErhI CCWWGG 3 cut(s) 198, 372, 457
FaeI CATG 2 cut(s) 229, 482
FatI CATG 2 cut(s) 225, 478
FokI GGATG 3 cut(s) 56, 224, 362
FspBI CTAG 3 cut(s) 189, 363, 495
Hin1II CATG 2 cut(s) 229, 482
HinfI GANTC 2 cut(s) 270, 521
Hpy188I TCNGA 2 cut(s) 400, 452
Hpy188III TCNNGA 2 cut(s) 508, 545
HpyCH4III ACNGT 2 cut(s) 518, 579
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 1 cut(s) 449
Hsp92II CATG 2 cut(s) 229, 482
LpnPI CCDG 6 cut(s) 166, 193, 193, 475, 502, 558
LweI GCATC 1 cut(s) 162
MaeI CTAG 3 cut(s) 189, 363, 495
MboII GAAGA 8 cut(s) 101, 116, 140, 143, 146, 389, 522, 566
MluCI AATT 3 cut(s) 71, 252, 428
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 7 cut(s) 34, 49, 52, 130, 177, 298, 418
MroXI GAANNNNTTC 1 cut(s) 413
MseI TTAA 2 cut(s) 74, 597
MspR9I CCNGG 2 cut(s) 181, 490
MvaI CCWGG 2 cut(s) 181, 490
MwoI GCNNNNNNNGC 1 cut(s) 158
NlaIII CATG 2 cut(s) 229, 482
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 270, 521
PshBI ATTAAT 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 179, 488
PspGI CCWGG 2 cut(s) 179, 488
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SaqAI TTAA 2 cut(s) 74, 597
ScrFI CCNGG 2 cut(s) 181, 490
SfaNI GCATC 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
SpeI ACTAGT 1 cut(s) 362
Sse9I AATT 3 cut(s) 71, 252, 428
SspMI CTAG 3 cut(s) 189, 363, 495
StyD4I CCNGG 2 cut(s) 179, 488
StyI CCWWGG 3 cut(s) 198, 372, 457
TaaI ACNGT 2 cut(s) 518, 579
TasI AATT 3 cut(s) 71, 252, 428
TfiI GAWTC 2 cut(s) 270, 521
Tru1I TTAA 2 cut(s) 74, 597
Tru9I TTAA 2 cut(s) 74, 597
TspDTI ATGAA 4 cut(s) 98, 102, 117, 141
VspI ATTAAT 1 cut(s) 597
XapI RAATTY 2 cut(s) 252, 428
XmnI GAANNNNTTC 1 cut(s) 413
XspI CTAG 3 cut(s) 189, 363, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.