Rorug03G0299800

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003873
Physical Location & Seq
Reverse (-)
31314497 .. 31315979
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0299800.1

Sequence Viewer

Length: 1314 bp
ATGGGTTCACCACCAACATGCACCCATTCACTGGTAAATATCACCTCAAGAAAGATCATTAGACCCTTGTCTCCAACTCCTCATCACCAAAGAACTCTAAACCTCTCTCTTTTGGACCAACTCTTCCCTCCTACCTTATACCCTACTATCATATATATCTACTCCATTGATCATTTTCCTTCAAATAATGTTTCGGAGAGCTTACAAACATCGCTATCCAAGGCTTTGGTTTACTTTTATCCCCTGGCCGGCCGACTAAATGGCCCTGCTTCCATCGACTGCAATGATGAAGGGGCTTATTTTCTTGAGGCCCAAGTCAACTGCCAGCTCTCGGACTTGCTTAAACTTCCTGAACACAGCTTACTCAACAACCTCATCCCGGAGTTTGATCCTCAAACCGCCCATTCCGCCATAGGTTCTGCAGTTTTGCTTGTCCAAATGAGTCGTTTTAACTGCGGAGGAATCGCCCTTGCTGTGTCGCTGTCGCACAAGATCGCAGATGCAACCTCATGTCAAACATTTGTACGAGCATGGGCTGCCATACATCGTGATCATAAATATGATACTCAACTAATGGTACCACAGTTTAATGGAGCATCTCTGTTGCCGGCTAAAGACTCCTTCCTAAACAACTTAGGGTTACCAAAGTATCAAAGTTTAACTACTAGGAGGTTCGTGTTTGATGTCACGAAGATTGCCAATCTCAAGGCTAAAATTGGAGGTACAAACGTACAACTTGTTTCAGCGATCATCTTGAAAAGTGCTCGTGCTGCTTCTTATCAATCCAAGCCGGAGACGAGTTCAAGTACAGCAGTGTTGTCGCAAATGGTAAATATCCGCAGAAGTATGGGTTCGGTTCTACAGGAAAATGCCATGGGAAACTGGTTTTGGCCACTTCAAGTGGTATTCAAACCAAACGAGACGGAACTACATGAGTTGGTGGCAAATATGAGGAGAGAACTAACAGACTTCTACAACGAGAAGGCGGGCAAATTCAAAGAGGAAGATGGGTTCTTGGTGATGTCTGAGTCGTTCAGAGAGAGGGGCGAGCTTTATAGAAGCACCAAGGGGTGCATCAGTGTTTATAGGGCCACAAGTTTGTGCAGGCTCCCTGTATATGAAATTGATTTTGGGTGGGGGAAACCAACATGGGTCACTTGCCAAAGCGACCAGAAGAACATATTTGTGTTGATTGATACAAAACGTGGCGATGGGATCGAAGCATGGGTGACACTCGATATAGAAGAAATGGCGATATTTGAGAGTGATGAGGAGCTCCTTTCATTCGCTTCTGTAAACCCCAGTGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

437

Amino Acids

48.75

Weight (kDa)

6.38

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 12 - 426 2.2e-75 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 577
AccB7I CCANNNNNTGG 1 cut(s) 31
AciI CCGC 5 cut(s) 399, 408, 456, 838, 986
AclWI GGATC 2 cut(s) 383, 1223
AcoI YGGCCR 3 cut(s) 246, 250, 890
AcsI RAATTY 1 cut(s) 992
AfaI GTAC 5 cut(s) 525, 579, 724, 732, 808
AfiI CCNNNNNNNGG 4 cut(s) 31, 248, 331, 379
AgsI TTSAA 6 cut(s) 183, 757, 804, 899, 910, 997
AjnI CCWGG 1 cut(s) 243
AjuI GAANNNNNNNTTGG 4 cut(s) 871, 903, 1113, 1145
AloI GAACNNNNNNTCC 2 cut(s) 995, 1027
AluBI AGCT 6 cut(s) 201, 328, 360, 1051, 1276, 1311
AluI AGCT 6 cut(s) 201, 328, 360, 1051, 1276, 1311
Alw21I GWGCWC 2 cut(s) 766, 1278
Alw26I GTCTC 3 cut(s) 75, 788, 914
AlwI GGATC 2 cut(s) 383, 1223
AoxI GGCC 6 cut(s) 246, 250, 262, 309, 890, 1089
ApeKI GCWGC 2 cut(s) 536, 770
ApoI RAATTY 1 cut(s) 992
Asp718I GGTACC 1 cut(s) 577
AspS9I GGNCC 4 cut(s) 115, 263, 310, 1089
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 4 cut(s) 34, 77, 1030, 1240
AsuNHI GCTAGC 1 cut(s) 1307
AvaII GGWCC 1 cut(s) 115
BaeI ACNNNNGTAYC 6 cut(s) 569, 602, 632, 665, 714, 747
BalI TGGCCA 1 cut(s) 892
BanI GGYRCC 1 cut(s) 577
BanII GRGCYC 1 cut(s) 1278
BauI CACGAG 1 cut(s) 765
Bbv12I GWGCWC 2 cut(s) 766, 1278
BbvI GCAGC 2 cut(s) 523, 757
BccI CCATC 3 cut(s) 281, 1001, 1205
BcgI CGANNNNNNTGC 2 cut(s) 801, 835
BciT130I CCWGG 1 cut(s) 245
BclI TGATCA 2 cut(s) 169, 550
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 3 cut(s) 75, 788, 914
BfaI CTAG 3 cut(s) 666, 1308, 1312
BfmI CTRYAG 2 cut(s) 420, 860
BisI GCNGC 2 cut(s) 537, 771
BlsI GCNGC 2 cut(s) 538, 772
Bme1390I CCNGG 2 cut(s) 245, 380
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 115, 263, 310, 1089
BmiI GGNNCC 2 cut(s) 579, 1109
BmrFI CCNGG 2 cut(s) 245, 380
BmrI ACTGGG 1 cut(s) 1296
BmsI GCATC 3 cut(s) 490, 605, 1083
BmtI GCTAGC 1 cut(s) 1311
BmuI ACTGGG 1 cut(s) 1296
BoxI GACNNNNGTC 1 cut(s) 67
BpuEI CTTGAG 3 cut(s) 31, 326, 689
BpuMI CCSGG 1 cut(s) 380
BsaJI CCNNGG 4 cut(s) 219, 243, 873, 1065
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 248, 331, 379
Bse118I RCCGGY 2 cut(s) 248, 607
Bse1I ACTGG 3 cut(s) 36, 887, 1302
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 4 cut(s) 219, 243, 873, 1065
BseGI GGATG 1 cut(s) 375
BseLI CCNNNNNNNGG 4 cut(s) 31, 248, 331, 379
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 1017
BseNI ACTGG 3 cut(s) 36, 887, 1302
BseRI GAGGAG 3 cut(s) 69, 967, 1286
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 2 cut(s) 523, 757
BsgI GTGCAG 1 cut(s) 1123
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 6 cut(s) 248, 252, 264, 311, 892, 1091
BshNI GGYRCC 1 cut(s) 577
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 2 cut(s) 766, 1278
BsiSI CCGG 4 cut(s) 249, 380, 608, 791
BslI CCNNNNNNNGG 4 cut(s) 31, 248, 331, 379
BsmAI GTCTC 3 cut(s) 75, 788, 914
BsmBI CGTCTC 2 cut(s) 788, 914
BsnI GGCC 6 cut(s) 248, 252, 264, 311, 892, 1091
Bsp1286I GDGCHC 2 cut(s) 766, 1278
Bsp143I GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
Bsp19I CCATGG 1 cut(s) 873
BspACI CCGC 5 cut(s) 399, 408, 456, 838, 986
BspANI GGCC 6 cut(s) 248, 252, 264, 311, 892, 1091
BspCNI CTCAG 1 cut(s) 1018
BspLI GGNNCC 2 cut(s) 579, 1109
BspMAI CTGCAG 1 cut(s) 424
BspOI GCTAGC 1 cut(s) 1311
BspPI GGATC 2 cut(s) 383, 1223
BspT107I GGYRCC 1 cut(s) 577
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 2 cut(s) 248, 607
BsrI ACTGG 3 cut(s) 36, 887, 1302
BssAI RCCGGY 2 cut(s) 248, 607
BssECI CCNNGG 4 cut(s) 219, 243, 873, 1065
BssMI GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
BssSI CACGAG 1 cut(s) 765
BssT1I CCWWGG 3 cut(s) 219, 873, 1065
Bst2BI CACGAG 1 cut(s) 765
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 585
Bst6I CTCTTC 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 536
BstC8I GCNNGC 7 cut(s) 250, 326, 609, 988, 1049, 1106, 1309
BstDEI CTNAG 2 cut(s) 634, 1026
BstDSI CCRYGG 1 cut(s) 873
BstEII GGTNACC 1 cut(s) 639
BstF5I GGATG 1 cut(s) 375
BstKTI GATC 7 cut(s) 57, 172, 391, 495, 553, 750, 1218
BstMAI GTCTC 3 cut(s) 75, 788, 914
BstMBI GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 3 cut(s) 407, 536, 770
BstNI CCWGG 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 21
BstPAI GACNNNNGTC 1 cut(s) 67
BstPI GGTNACC 1 cut(s) 639
BstSCI CCNGG 2 cut(s) 243, 378
BstSFI CTRYAG 2 cut(s) 420, 860
BstV1I GCAGC 2 cut(s) 523, 757
BstXI CCANNNNNNTGG 1 cut(s) 226
BstZI CGGCCG 1 cut(s) 250
BsuRI GGCC 6 cut(s) 248, 252, 264, 311, 892, 1091
BtgI CCRYGG 1 cut(s) 873
BtgZI GCGATG 2 cut(s) 195, 1224
BtsCI GGATG 1 cut(s) 375
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 4 cut(s) 29, 819, 1084, 1309
Cac8I GCNNGC 7 cut(s) 250, 326, 609, 988, 1049, 1106, 1309
Cfr10I RCCGGY 2 cut(s) 248, 607
Cfr13I GGNCC 4 cut(s) 115, 263, 310, 1089
Csp6I GTAC 5 cut(s) 524, 578, 723, 731, 807
CviAII CATG 7 cut(s) 18, 510, 531, 874, 932, 1149, 1224
CviQI GTAC 5 cut(s) 524, 578, 723, 731, 807
DdeI CTNAG 2 cut(s) 634, 1026
DpnI GATC 7 cut(s) 56, 171, 390, 494, 552, 749, 1217
DpnII GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
EaeI YGGCCR 3 cut(s) 246, 250, 890
EagI CGGCCG 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
EciI GGCGGA 1 cut(s) 397
Ecl136II GAGCTC 1 cut(s) 1276
EclXI CGGCCG 1 cut(s) 250
Eco130I CCWWGG 3 cut(s) 219, 873, 1065
Eco24I GRGCYC 1 cut(s) 1278
Eco47I GGWCC 1 cut(s) 115
Eco52I CGGCCG 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 1276
Eco91I GGTNACC 1 cut(s) 639
EcoICRI GAGCTC 1 cut(s) 1276
EcoO65I GGTNACC 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 3 cut(s) 219, 873, 1065
EcoT38I GRGCYC 1 cut(s) 1278
ErhI CCWWGG 3 cut(s) 219, 873, 1065
Esp3I CGTCTC 2 cut(s) 788, 914
FaeI CATG 7 cut(s) 21, 513, 534, 877, 935, 1152, 1227
FatI CATG 7 cut(s) 17, 509, 530, 873, 931, 1148, 1223
FauI CCCGC 1 cut(s) 979
FbaI TGATCA 2 cut(s) 169, 550
Fnu4HI GCNGC 2 cut(s) 537, 771
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 1278
FseI GGCCGGCC 1 cut(s) 252
Fsp4HI GCNGC 2 cut(s) 537, 771
FspBI CTAG 3 cut(s) 666, 1308, 1312
GluI GCNGC 2 cut(s) 537, 771
HaeIII GGCC 6 cut(s) 248, 252, 264, 311, 892, 1091
HapII CCGG 4 cut(s) 249, 380, 608, 791
Hin1II CATG 7 cut(s) 21, 513, 534, 877, 935, 1152, 1227
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 4 cut(s) 442, 462, 617, 1028
HpaII CCGG 4 cut(s) 249, 380, 608, 791
HphI GGTGA 4 cut(s) 34, 77, 1030, 1240
Hpy166II GTNNAC 4 cut(s) 8, 232, 319, 1297
Hpy188I TCNGA 4 cut(s) 196, 334, 1027, 1037
Hpy188III TCNNGA 6 cut(s) 48, 305, 350, 548, 688, 754
Hpy8I GTNNAC 4 cut(s) 8, 232, 319, 1297
HpyAV CCTTC 4 cut(s) 189, 284, 631, 976
HpyCH4III ACNGT 1 cut(s) 585
HpyCH4IV ACGT 2 cut(s) 729, 1204
HpyCH4V TGCA 6 cut(s) 21, 282, 422, 503, 1074, 1104
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 536, 770
HpyF3I CTNAG 2 cut(s) 634, 1026
HpySE526I ACGT 2 cut(s) 729, 1204
Hsp92II CATG 7 cut(s) 21, 513, 534, 877, 935, 1152, 1227
KpnI GGTACC 1 cut(s) 581
KroI GCCGGC 2 cut(s) 248, 607
KroNI GCCGGC 2 cut(s) 250, 609
Ksp22I TGATCA 2 cut(s) 169, 550
Kzo9I GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
LmnI GCTCC 4 cut(s) 593, 1113, 1273, 1281
Lsp1109I GCAGC 2 cut(s) 523, 757
LweI GCATC 3 cut(s) 490, 605, 1083
MaeI CTAG 3 cut(s) 666, 1308, 1312
MaeII ACGT 2 cut(s) 729, 1204
MaeIII GTNAC 4 cut(s) 639, 685, 1153, 1228
MalI GATC 7 cut(s) 56, 171, 390, 494, 552, 749, 1217
MboI GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
MboII GAAGA 5 cut(s) 115, 703, 1016, 1186, 1256
MhlI GDGCHC 2 cut(s) 766, 1278
MlsI TGGCCA 1 cut(s) 892
MluCI AATT 3 cut(s) 714, 992, 1122
MluNI TGGCCA 1 cut(s) 892
MlyI GAGTC 3 cut(s) 451, 611, 1037
MmeI TCCRAC 1 cut(s) 98
Mox20I TGGCCA 1 cut(s) 892
MroNI GCCGGC 2 cut(s) 248, 607
MscI TGGCCA 1 cut(s) 892
MseI TTAA 4 cut(s) 342, 450, 588, 659
MslI CAYNNNNRTG 3 cut(s) 16, 558, 1184
Msp20I TGGCCA 1 cut(s) 892
MspI CCGG 4 cut(s) 249, 380, 608, 791
MspR9I CCNGG 2 cut(s) 245, 380
MvaI CCWGG 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 407, 536, 770
NaeI GCCGGC 2 cut(s) 250, 609
NciI CCSGG 1 cut(s) 380
NcoI CCATGG 1 cut(s) 873
NdeII GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
NgoMIV GCCGGC 2 cut(s) 248, 607
NheI GCTAGC 1 cut(s) 1307
NlaIII CATG 7 cut(s) 21, 513, 534, 877, 935, 1152, 1227
NlaIV GGNNCC 2 cut(s) 579, 1109
NmuCI GTSAC 3 cut(s) 685, 1153, 1228
NspI RCATGY 1 cut(s) 21
PdiI GCCGGC 2 cut(s) 250, 609
PfeI GAWTC 1 cut(s) 462
PflMI CCANNNNNTGG 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 2 cut(s) 538, 772
PleI GAGTC 3 cut(s) 450, 611, 1036
PpsI GAGTC 3 cut(s) 450, 611, 1036
PshAI GACNNNNGTC 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 1278
Psp6I CCWGG 1 cut(s) 243
PspEI GGTNACC 1 cut(s) 639
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 579, 1109
PspPI GGNCC 4 cut(s) 115, 263, 310, 1089
PsrI GAACNNNNNNTAC 2 cut(s) 345, 377
PstI CTGCAG 1 cut(s) 424
RigI GGCCGGCC 1 cut(s) 252
RsaI GTAC 5 cut(s) 525, 579, 724, 732, 808
RsaNI GTAC 5 cut(s) 524, 578, 723, 731, 807
RseI CAYNNNNRTG 3 cut(s) 16, 558, 1184
SacI GAGCTC 1 cut(s) 1278
SaqAI TTAA 4 cut(s) 342, 450, 588, 659
SatI GCNGC 2 cut(s) 537, 771
Sau3AI GATC 7 cut(s) 54, 169, 388, 492, 550, 747, 1215
Sau96I GGNCC 4 cut(s) 115, 263, 310, 1089
SchI GAGTC 3 cut(s) 451, 611, 1037
ScrFI CCNGG 2 cut(s) 245, 380
SduI GDGCHC 2 cut(s) 766, 1278
SfaNI GCATC 3 cut(s) 490, 605, 1083
SfcI CTRYAG 2 cut(s) 420, 860
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 3 cut(s) 16, 558, 1184
SmlI CTYRAG 3 cut(s) 46, 305, 704
SmoI CTYRAG 3 cut(s) 46, 305, 704
Sse9I AATT 3 cut(s) 714, 992, 1122
SsiI CCGC 5 cut(s) 399, 408, 456, 838, 986
SspMI CTAG 3 cut(s) 666, 1308, 1312
SstI GAGCTC 1 cut(s) 1278
StyD4I CCNGG 2 cut(s) 243, 378
StyI CCWWGG 3 cut(s) 219, 873, 1065
TaaI ACNGT 1 cut(s) 585
TaiI ACGT 2 cut(s) 732, 1207
TaqI TCGA 3 cut(s) 276, 1218, 1236
TasI AATT 3 cut(s) 714, 992, 1122
TatI WGTACW 1 cut(s) 806
TfiI GAWTC 1 cut(s) 462
Tru1I TTAA 4 cut(s) 342, 450, 588, 659
Tru9I TTAA 4 cut(s) 342, 450, 588, 659
TscAI CASTG 4 cut(s) 36, 819, 1084, 1309
TseFI GTSAC 3 cut(s) 685, 1153, 1228
TseI GCWGC 2 cut(s) 536, 770
Tsp45I GTSAC 3 cut(s) 685, 1153, 1228
TspDTI ATGAA 3 cut(s) 303, 1134, 1272
TspGWI ACGGA 1 cut(s) 938
TspRI CASTG 4 cut(s) 36, 819, 1084, 1309
Van91I CCANNNNNTGG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 1 cut(s) 992
XceI RCATGY 1 cut(s) 21
XspI CTAG 3 cut(s) 666, 1308, 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.