Rorug03G0311400

Plant pleckstrin homology-like region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004295
Physical Location & Seq
Reverse (-)
33280499 .. 33281682
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0311400.1

Sequence Viewer

Length: 807 bp
ATGTATTCCTCGATTTTCACATCCTCTCACCCCATGACCCAAGAGCCACGAACTCTGCAACTAATGGAGCTCCAAACCCCGCAACAAATGGAACCACAACGACAGCAACCAACAGAGGAGGGAGGAAACACCCAAGCAGCTGGAAGTAGTGCCAACGTGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGAACAAATAAGAATCCTCAAGGATCTTTACTACGTCAAGGGATTTAAGTGCCCAACTACAGAGCAGATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGGTATGTTGAGGGTAAGAACATTTACTTTTGGTTCCAGAACGTCAGGGCTCGAGAGAAGCAGATGAATAGGTGTAATCAGGCTGCTCCAGTGCCCATGGGAACTAGTTCTCTTGGTACTGGTAGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTGGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATGATGGTAGATCCATTGATCTCAACTTTGGTTCCACCTATTCTACTGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGGTTCAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGGACGTCTTTGGTAACCTGAAGACTACTTCCGAGGAAGGTAGTGCACATGATTACTATTTCGGTGGCTCAGGCGGTTACAACCGTGGATCTCCAGTTTCTCTTGAGCTCAGCCTCAACCCATCCGGAGCTGCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_canalis PH_2

Protein Analysis

268

Amino Acids

29.23

Weight (kDa)

5.14

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 59 - 120 6.5e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 675
AccB7I CCANNNNNTGG 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 791
AciI CCGC 2 cut(s) 80, 741
AclWI GGATC 8 cut(s) 228, 417, 465, 478, 498, 511, 546, 763
AcuI CTGAAG 1 cut(s) 707
AcyI GRCGYC 1 cut(s) 672
AfaI GTAC 2 cut(s) 178, 415
AfiI CCNNNNNNNGG 2 cut(s) 500, 662
AgsI TTSAA 1 cut(s) 638
AhlI ACTAGT 2 cut(s) 401, 530
AjnI CCWGG 2 cut(s) 179, 627
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 6 cut(s) 70, 140, 286, 497, 775, 797
AluI AGCT 6 cut(s) 70, 140, 286, 497, 775, 797
Alw21I GWGCWC 4 cut(s) 72, 663, 715, 777
Alw26I GTCTC 1 cut(s) 284
Alw44I GTGCAC 2 cut(s) 659, 711
AlwI GGATC 8 cut(s) 228, 417, 465, 478, 498, 511, 546, 763
Ama87I CYCGRG 1 cut(s) 348
Aor13HI TCCGGA 1 cut(s) 791
AoxI GGCC 1 cut(s) 491
ApaLI GTGCAC 2 cut(s) 659, 711
ApeKI GCWGC 3 cut(s) 137, 380, 797
ArsI GACNNNNNNTTYG 4 cut(s) 470, 502, 503, 535
Asp700I GAANNNNTTC 1 cut(s) 403
AspS9I GGNCC 3 cut(s) 444, 491, 525
AsuHPI GGTGA 2 cut(s) 20, 617
AvaI CYCGRG 1 cut(s) 348
AvaII GGWCC 2 cut(s) 444, 525
BaeGI GKGCMC 4 cut(s) 251, 393, 663, 715
BamHI GGATCC 2 cut(s) 470, 503
BanII GRGCYC 3 cut(s) 72, 349, 777
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 695
Bbv12I GWGCWC 4 cut(s) 72, 663, 715, 777
BbvI GCAGC 3 cut(s) 149, 367, 784
BccI CCATC 3 cut(s) 482, 539, 796
BceAI ACGGC 2 cut(s) 159, 679
BciT130I CCWGG 2 cut(s) 181, 629
BcoDI GTCTC 1 cut(s) 284
BcuI ACTAGT 2 cut(s) 401, 530
BfaI CTAG 3 cut(s) 402, 531, 805
BfmI CTRYAG 1 cut(s) 255
BglII AGATCT 1 cut(s) 273
BisI GCNGC 3 cut(s) 138, 381, 798
BlpI GCTNAGC 1 cut(s) 776
BlsI GCNGC 3 cut(s) 139, 382, 799
Bme1390I CCNGG 2 cut(s) 181, 629
Bme18I GGWCC 2 cut(s) 444, 525
BmeT110I CYCGRG 1 cut(s) 348
BmgT120I GGNCC 3 cut(s) 444, 491, 525
BmiI GGNNCC 7 cut(s) 93, 332, 445, 472, 505, 526, 574
BmrFI CCNGG 2 cut(s) 181, 629
BpiI GAAGAC 1 cut(s) 695
BpmI CTGGAG 5 cut(s) 180, 266, 369, 609, 744
Bpu10I CCTNAGC 1 cut(s) 736
Bpu1102I GCTNAGC 1 cut(s) 776
BpuEI CTTGAG 2 cut(s) 200, 791
BsaHI GRCGYC 1 cut(s) 672
BsaJI CCNNGG 3 cut(s) 393, 699, 751
BsaWI WCCGGW 1 cut(s) 791
Bsc4I CCNNNNNNNGG 2 cut(s) 500, 662
Bse1I ACTGG 7 cut(s) 292, 386, 421, 454, 463, 592, 761
BseAI TCCGGA 1 cut(s) 791
BseBI CCWGG 2 cut(s) 181, 629
BseDI CCNNGG 3 cut(s) 393, 699, 751
BseGI GGATG 3 cut(s) 20, 599, 788
BseLI CCNNNNNNNGG 2 cut(s) 500, 662
BseMII CTCAG 2 cut(s) 750, 790
BseNI ACTGG 7 cut(s) 292, 386, 421, 454, 463, 592, 761
BseRI GAGGAG 3 cut(s) 131, 630, 633
BseSI GKGCMC 4 cut(s) 251, 393, 663, 715
BseXI GCAGC 3 cut(s) 149, 367, 784
BshFI GGCC 1 cut(s) 493
BsiHKAI GWGCWC 4 cut(s) 72, 663, 715, 777
BsiHKCI CYCGRG 1 cut(s) 348
BsiSI CCGG 1 cut(s) 792
BslI CCNNNNNNNGG 2 cut(s) 500, 662
BsmAI GTCTC 1 cut(s) 284
BsnI GGCC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 348
Bsp1286I GDGCHC 7 cut(s) 72, 251, 349, 393, 663, 715, 777
Bsp13I TCCGGA 1 cut(s) 791
Bsp1720I GCTNAGC 1 cut(s) 776
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 2 cut(s) 80, 741
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 2 cut(s) 749, 789
BspEI TCCGGA 1 cut(s) 791
BspLI GGNNCC 7 cut(s) 93, 332, 445, 472, 505, 526, 574
BspPI GGATC 8 cut(s) 228, 417, 465, 478, 498, 511, 546, 763
BsrI ACTGG 7 cut(s) 292, 386, 421, 454, 463, 592, 761
BssECI CCNNGG 3 cut(s) 393, 699, 751
BssNI GRCGYC 1 cut(s) 672
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 181, 629
Bst4CI ACNGT 1 cut(s) 752
Bst6I CTCTTC 1 cut(s) 648
BstACI GRCGYC 1 cut(s) 672
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 2 cut(s) 736, 776
BstDSI CCRYGG 2 cut(s) 393, 751
BstEII GGTNACC 1 cut(s) 680
BstF5I GGATG 3 cut(s) 20, 599, 788
BstMAI GTCTC 1 cut(s) 284
BstNI CCWGG 2 cut(s) 181, 629
BstPI GGTNACC 1 cut(s) 680
BstSCI CCNGG 2 cut(s) 179, 627
BstSFI CTRYAG 1 cut(s) 255
BstSLI GKGCMC 4 cut(s) 251, 393, 663, 715
BstV1I GCAGC 3 cut(s) 149, 367, 784
BstV2I GAAGAC 1 cut(s) 695
BstX2I RGATCY 7 cut(s) 220, 273, 422, 470, 503, 551, 755
BstXI CCANNNNNNTGG 1 cut(s) 140
BstYI RGATCY 7 cut(s) 220, 273, 422, 470, 503, 551, 755
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 2 cut(s) 393, 751
BtsCI GGATG 3 cut(s) 20, 599, 788
BtsIMutI CAGTG 2 cut(s) 393, 447
Cac8I GCNNGC 1 cut(s) 495
Cfr13I GGNCC 3 cut(s) 444, 491, 525
CsiI ACCWGGT 1 cut(s) 179
Csp6I GTAC 2 cut(s) 177, 414
CviAII CATG 3 cut(s) 34, 394, 716
CviQI GTAC 2 cut(s) 177, 414
DdeI CTNAG 2 cut(s) 736, 776
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Ecl136II GAGCTC 2 cut(s) 70, 775
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 3 cut(s) 72, 349, 777
Eco47I GGWCC 2 cut(s) 444, 525
Eco53kI GAGCTC 2 cut(s) 70, 775
Eco57I CTGAAG 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 348
Eco91I GGTNACC 1 cut(s) 680
EcoICRI GAGCTC 2 cut(s) 70, 775
EcoO65I GGTNACC 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 179, 627
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 3 cut(s) 72, 349, 777
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 3 cut(s) 37, 397, 719
FaiI YATR 5 cut(s) 35, 297, 303, 395, 717
FatI CATG 3 cut(s) 33, 393, 715
FauI CCCGC 1 cut(s) 87
Fnu4HI GCNGC 3 cut(s) 138, 381, 798
FokI GGATG 3 cut(s) 7, 586, 775
FriOI GRGCYC 3 cut(s) 72, 349, 777
Fsp4HI GCNGC 3 cut(s) 138, 381, 798
FspBI CTAG 3 cut(s) 402, 531, 805
GluI GCNGC 3 cut(s) 138, 381, 798
GsuI CTGGAG 5 cut(s) 180, 266, 369, 609, 744
HaeIII GGCC 1 cut(s) 493
HapII CCGG 1 cut(s) 792
Hin1I GRCGYC 1 cut(s) 672
Hin1II CATG 3 cut(s) 37, 397, 719
HinfI GANTC 3 cut(s) 187, 210, 265
HpaII CCGG 1 cut(s) 792
HphI GGTGA 2 cut(s) 20, 617
Hpy166II GTNNAC 4 cut(s) 447, 528, 661, 713
Hpy188I TCNGA 1 cut(s) 700
Hpy188III TCNNGA 6 cut(s) 197, 269, 334, 350, 770, 792
Hpy8I GTNNAC 4 cut(s) 447, 528, 661, 713
HpyAV CCTTC 1 cut(s) 698
HpyCH4III ACNGT 1 cut(s) 752
HpyCH4IV ACGT 4 cut(s) 156, 231, 339, 672
HpyCH4V TGCA 4 cut(s) 58, 607, 661, 713
HpyF3I CTNAG 2 cut(s) 736, 776
HpySE526I ACGT 4 cut(s) 156, 231, 339, 672
Hsp92I GRCGYC 1 cut(s) 672
Hsp92II CATG 3 cut(s) 37, 397, 719
Kpn2I TCCGGA 1 cut(s) 791
LmnI GCTCC 4 cut(s) 67, 75, 388, 794
Lsp1109I GCAGC 3 cut(s) 149, 367, 784
MabI ACCWGGT 1 cut(s) 179
MaeI CTAG 3 cut(s) 402, 531, 805
MaeII ACGT 4 cut(s) 156, 231, 339, 672
MaeIII GTNAC 2 cut(s) 680, 743
MboII GAAGA 2 cut(s) 635, 700
MflI RGATCY 7 cut(s) 220, 273, 422, 470, 503, 551, 755
MhlI GDGCHC 7 cut(s) 72, 251, 349, 393, 663, 715, 777
MluCI AATT 3 cut(s) 436, 484, 517
MroI TCCGGA 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 403
MseI TTAA 1 cut(s) 243
MspA1I CMGCKG 3 cut(s) 140, 286, 497
MspI CCGG 1 cut(s) 792
MspR9I CCNGG 2 cut(s) 181, 629
MvaI CCWGG 2 cut(s) 181, 629
NcoI CCATGG 1 cut(s) 393
NlaIII CATG 3 cut(s) 37, 397, 719
NlaIV GGNNCC 7 cut(s) 93, 332, 445, 472, 505, 526, 574
PaeR7I CTCGAG 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 403
PfeI GAWTC 3 cut(s) 187, 210, 265
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 3 cut(s) 139, 382, 799
Psp124BI GAGCTC 2 cut(s) 72, 777
Psp6I CCWGG 2 cut(s) 179, 627
PspEI GGTNACC 1 cut(s) 680
PspGI CCWGG 2 cut(s) 179, 627
PspN4I GGNNCC 7 cut(s) 93, 332, 445, 472, 505, 526, 574
PspPI GGNCC 3 cut(s) 444, 491, 525
PsuI RGATCY 7 cut(s) 220, 273, 422, 470, 503, 551, 755
PvuII CAGCTG 3 cut(s) 140, 286, 497
RsaI GTAC 2 cut(s) 178, 415
RsaNI GTAC 2 cut(s) 177, 414
SacI GAGCTC 2 cut(s) 72, 777
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 3 cut(s) 138, 381, 798
Sau96I GGNCC 3 cut(s) 444, 491, 525
ScrFI CCNGG 2 cut(s) 181, 629
SduI GDGCHC 7 cut(s) 72, 251, 349, 393, 663, 715, 777
SexAI ACCWGGT 1 cut(s) 179
SfcI CTRYAG 1 cut(s) 255
Sfr274I CTCGAG 1 cut(s) 348
SinI GGWCC 2 cut(s) 444, 525
SlaI CTCGAG 1 cut(s) 348
SmlI CTYRAG 3 cut(s) 215, 348, 770
SmoI CTYRAG 3 cut(s) 215, 348, 770
SpeI ACTAGT 2 cut(s) 401, 530
Sse9I AATT 3 cut(s) 436, 484, 517
SsiI CCGC 2 cut(s) 80, 741
SspMI CTAG 3 cut(s) 402, 531, 805
SstI GAGCTC 2 cut(s) 72, 777
StyD4I CCNGG 2 cut(s) 179, 627
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 752
TaiI ACGT 4 cut(s) 159, 234, 342, 675
TaqI TCGA 3 cut(s) 11, 349, 477
TasI AATT 3 cut(s) 436, 484, 517
TfiI GAWTC 3 cut(s) 187, 210, 265
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 2 cut(s) 393, 454
TseI GCWGC 3 cut(s) 137, 380, 797
TspDTI ATGAA 1 cut(s) 377
TspRI CASTG 2 cut(s) 393, 454
Van91I CCANNNNNTGG 1 cut(s) 500
VneI GTGCAC 2 cut(s) 659, 711
VpaK11BI GGWCC 2 cut(s) 444, 525
XhoI CTCGAG 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 403
XspI CTAG 3 cut(s) 402, 531, 805
ZraI GACGTC 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.