Rorug03G0313300

Dymeclin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008749
Physical Location & Seq
Forward (+)
33559156 .. 33559907
752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0313300.1

Sequence Viewer

Length: 642 bp
ATGGGAACTCCAGAAACATCTCGGGAGCCGTGCCCGGACAGAATCCTCGACGACGTGGGCGGAGCCTTCGGGATGGGGGCCGTCGGAGGGGCGGCCTTCCACTTCATCAAAGGCATCTACAACTCCCCCAAGGGCGCGCGTCTCGTCGGAGGCTCCCAGGCCGTCCGCATGAACGCCCCCCGCGTCGGCGGAAGCTTCGCCGTCTGGGGCGGACTCTTCTCCACCTTCGACTGCACCATGGTCTACCTCCGCCAGAAAGAGGACCCCTGGAACTCCATCATCGCCGGCGCGGCCACCGGAGGCTTCCTCCAGATGCGGCAGGGCCTCGGCGCCACCGCGAGGTCCGCCGCCATGGGAGGGGTTCTGTTGGCCCTGATCGAAGGCGCCGGGATCATGCTCAACAAAATCATGAGCGAGCAGCAACAGCAGCAGCAGATGGTGATGGAGGATCCTGGTGCTGCTCTGCCTGGGATGCCGCAGATGGGCGGGCCTCCCGGCTCAGAAGAAACCTCCTCCGTTGGGTCGTGGTTGGGAGGGTGGTTTGGGAAAGCCAAAGAGCCTGAGCCGGCCAGCAGCGGAAGCGAGACCAAGGTCCTCGAGAGTTTTGATGCGCCTCCAGTGCCCTCTTTTGAGTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dymeclin

Protein Analysis

213

Amino Acids

22.16

Weight (kDa)

5.08

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 15 - 122 2.6e-31 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 329, 383
AccI GTMKAC 1 cut(s) 243
AccII CGCG 5 cut(s) 137, 139, 183, 290, 338
AclWI GGATC 3 cut(s) 398, 443, 456
AcoI YGGCCR 2 cut(s) 291, 567
AcyI GRCGYC 2 cut(s) 330, 384
AfiI CCNNNNNNNGG 7 cut(s) 87, 185, 259, 339, 357, 482, 519
AgsI TTSAA 1 cut(s) 637
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 4 cut(s) 156, 266, 451, 466
AluBI AGCT 1 cut(s) 195
AluI AGCT 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 146, 578
AlwI GGATC 3 cut(s) 398, 443, 456
Ama87I CYCGRG 2 cut(s) 21, 596
AoxI GGCC 8 cut(s) 78, 93, 159, 291, 322, 369, 488, 567
ApeKI GCWGC 5 cut(s) 418, 427, 430, 458, 573
AspLEI GCGC 6 cut(s) 137, 139, 290, 332, 386, 613
AspS9I GGNCC 7 cut(s) 78, 262, 322, 342, 370, 488, 592
AsuC2I CCSGG 3 cut(s) 35, 388, 495
AsuHPI GGTGA 1 cut(s) 451
AvaI CYCGRG 2 cut(s) 21, 596
AvaII GGWCC 3 cut(s) 262, 342, 592
BaeGI GKGCMC 2 cut(s) 35, 624
BamHI GGATCC 1 cut(s) 448
BanI GGYRCC 2 cut(s) 329, 383
BbvI GCAGC 5 cut(s) 430, 439, 442, 445, 585
BccI CCATC 5 cut(s) 67, 284, 430, 436, 475
BceAI ACGGC 4 cut(s) 13, 65, 146, 185
BciT130I CCWGG 4 cut(s) 158, 268, 453, 468
BcnI CCSGG 3 cut(s) 35, 388, 495
BcoDI GTCTC 2 cut(s) 146, 578
BfoI RGCGCY 2 cut(s) 333, 387
BglI GCCNNNNNGGC 1 cut(s) 290
Bme1390I CCNGG 7 cut(s) 35, 158, 268, 388, 453, 468, 495
Bme18I GGWCC 3 cut(s) 262, 342, 592
BmeT110I CYCGRG 2 cut(s) 21, 596
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 7 cut(s) 78, 262, 322, 342, 370, 488, 592
BmiI GGNNCC 8 cut(s) 27, 64, 79, 154, 264, 331, 385, 450
BmrFI CCNGG 7 cut(s) 35, 158, 268, 388, 453, 468, 495
BmsI GCATC 4 cut(s) 123, 303, 462, 598
BoxI GACNNNNGTC 1 cut(s) 590
BplI GAGNNNNNCTC 2 cut(s) 291, 323
BpmI CTGGAG 2 cut(s) 293, 600
Bpu10I CCTNAGC 1 cut(s) 561
BpuMI CCSGG 3 cut(s) 35, 388, 495
BsaHI GRCGYC 2 cut(s) 330, 384
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 8 cut(s) 129, 156, 237, 266, 325, 351, 467, 588
BsaWI WCCGGW 1 cut(s) 296
Bsc4I CCNNNNNNNGG 7 cut(s) 87, 185, 259, 339, 357, 482, 519
Bse118I RCCGGY 2 cut(s) 284, 565
Bse1I ACTGG 1 cut(s) 617
BseBI CCWGG 4 cut(s) 158, 268, 453, 468
BseDI CCNNGG 8 cut(s) 129, 156, 237, 266, 325, 351, 467, 588
BseGI GGATG 2 cut(s) 78, 477
BseLI CCNNNNNNNGG 7 cut(s) 87, 185, 259, 339, 357, 482, 519
BseMII CTCAG 2 cut(s) 513, 552
BseNI ACTGG 1 cut(s) 617
BsePI GCGCGC 1 cut(s) 135
BseRI GAGGAG 1 cut(s) 502
BseSI GKGCMC 2 cut(s) 35, 624
BseXI GCAGC 5 cut(s) 430, 439, 442, 445, 585
BsgI GTGCAG 1 cut(s) 217
Bsh1236I CGCG 5 cut(s) 137, 139, 183, 290, 338
BshFI GGCC 8 cut(s) 80, 95, 161, 293, 324, 371, 490, 569
BshNI GGYRCC 2 cut(s) 329, 383
BsiHKCI CYCGRG 2 cut(s) 21, 596
BsiSI CCGG 6 cut(s) 35, 285, 297, 387, 495, 566
BslI CCNNNNNNNGG 7 cut(s) 87, 185, 259, 339, 357, 482, 519
BsmAI GTCTC 2 cut(s) 146, 578
BsmBI CGTCTC 1 cut(s) 146
BsnI GGCC 8 cut(s) 80, 95, 161, 293, 324, 371, 490, 569
Bso31I GGTCTC 1 cut(s) 578
BsoBI CYCGRG 2 cut(s) 21, 596
Bsp1286I GDGCHC 2 cut(s) 35, 624
Bsp143I GATC 3 cut(s) 375, 390, 448
Bsp19I CCATGG 2 cut(s) 237, 351
BspANI GGCC 8 cut(s) 80, 95, 161, 293, 324, 371, 490, 569
BspCNI CTCAG 2 cut(s) 512, 553
BspFNI CGCG 5 cut(s) 137, 139, 183, 290, 338
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 8 cut(s) 27, 64, 79, 154, 264, 331, 385, 450
BspPI GGATC 3 cut(s) 398, 443, 456
BspT107I GGYRCC 2 cut(s) 329, 383
BspTNI GGTCTC 1 cut(s) 578
BsrFI RCCGGY 2 cut(s) 284, 565
BsrI ACTGG 1 cut(s) 617
BssAI RCCGGY 2 cut(s) 284, 565
BssECI CCNNGG 8 cut(s) 129, 156, 237, 266, 325, 351, 467, 588
BssHII GCGCGC 1 cut(s) 135
BssMI GATC 3 cut(s) 375, 390, 448
BssNI GRCGYC 2 cut(s) 330, 384
BssT1I CCWWGG 4 cut(s) 129, 237, 351, 588
Bst2UI CCWGG 4 cut(s) 158, 268, 453, 468
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 2 cut(s) 330, 384
BstC8I GCNNGC 6 cut(s) 137, 286, 416, 488, 567, 571
BstDEI CTNAG 2 cut(s) 499, 561
BstDSI CCRYGG 2 cut(s) 237, 351
BstF5I GGATG 2 cut(s) 78, 477
BstFNI CGCG 5 cut(s) 137, 139, 183, 290, 338
BstH2I RGCGCY 2 cut(s) 333, 387
BstHHI GCGC 6 cut(s) 137, 139, 290, 332, 386, 613
BstKTI GATC 3 cut(s) 378, 393, 451
BstMAI GTCTC 2 cut(s) 146, 578
BstMBI GATC 3 cut(s) 375, 390, 448
BstMWI GCNNNNNNNGC 7 cut(s) 290, 344, 424, 427, 472, 579, 619
BstNI CCWGG 4 cut(s) 158, 268, 453, 468
BstPAI GACNNNNGTC 1 cut(s) 590
BstSCI CCNGG 7 cut(s) 33, 156, 266, 386, 451, 466, 493
BstSLI GKGCMC 2 cut(s) 35, 624
BstUI CGCG 5 cut(s) 137, 139, 183, 290, 338
BstV1I GCAGC 5 cut(s) 430, 439, 442, 445, 585
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 8 cut(s) 80, 95, 161, 293, 324, 371, 490, 569
BtgI CCRYGG 2 cut(s) 237, 351
BtgZI GCGATG 1 cut(s) 265
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 2 cut(s) 78, 477
BtsIMutI CAGTG 1 cut(s) 624
Cac8I GCNNGC 6 cut(s) 137, 286, 416, 488, 567, 571
CciI TCATGA 1 cut(s) 408
CfoI GCGC 6 cut(s) 137, 139, 290, 332, 386, 613
Cfr10I RCCGGY 2 cut(s) 284, 565
Cfr13I GGNCC 7 cut(s) 78, 262, 322, 342, 370, 488, 592
CseI GACGC 2 cut(s) 128, 172
CviAII CATG 5 cut(s) 169, 238, 352, 394, 409
DdeI CTNAG 2 cut(s) 499, 561
DinI GGCGCC 2 cut(s) 331, 385
DpnI GATC 3 cut(s) 377, 392, 450
DpnII GATC 3 cut(s) 375, 390, 448
EaeI YGGCCR 2 cut(s) 291, 567
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
EciI GGCGGA 5 cut(s) 75, 204, 225, 239, 334
Eco130I CCWWGG 4 cut(s) 129, 237, 351, 588
Eco31I GGTCTC 1 cut(s) 578
Eco47I GGWCC 3 cut(s) 262, 342, 592
Eco88I CYCGRG 2 cut(s) 21, 596
EcoO109I RGGNCCY 3 cut(s) 262, 322, 592
EcoRII CCWGG 4 cut(s) 156, 266, 451, 466
EcoT14I CCWWGG 4 cut(s) 129, 237, 351, 588
EgeI GGCGCC 2 cut(s) 331, 385
EheI GGCGCC 2 cut(s) 331, 385
ErhI CCWWGG 4 cut(s) 129, 237, 351, 588
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 5 cut(s) 172, 241, 355, 397, 412
FaiI YATR 5 cut(s) 170, 239, 353, 395, 410
FatI CATG 5 cut(s) 168, 237, 351, 393, 408
FauI CCCGC 2 cut(s) 188, 479
FblI GTMKAC 1 cut(s) 243
FokI GGATG 2 cut(s) 85, 484
GlaI GCGC 6 cut(s) 136, 138, 289, 331, 385, 612
GsuI CTGGAG 2 cut(s) 293, 600
HaeII RGCGCY 2 cut(s) 333, 387
HaeIII GGCC 8 cut(s) 80, 95, 161, 293, 324, 371, 490, 569
HapII CCGG 6 cut(s) 35, 285, 297, 387, 495, 566
HgaI GACGC 2 cut(s) 128, 172
HhaI GCGC 6 cut(s) 137, 139, 290, 332, 386, 613
Hin1I GRCGYC 2 cut(s) 330, 384
Hin1II CATG 5 cut(s) 172, 241, 355, 397, 412
Hin6I GCGC 6 cut(s) 135, 137, 288, 330, 384, 611
HinP1I GCGC 6 cut(s) 135, 137, 288, 330, 384, 611
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 2 cut(s) 42, 213
HpaII CCGG 6 cut(s) 35, 285, 297, 387, 495, 566
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 3 cut(s) 86, 149, 502
Hpy188III TCNNGA 6 cut(s) 11, 23, 70, 310, 409, 598
Hpy8I GTNNAC 1 cut(s) 244
Hpy99I CGWCG 5 cut(s) 53, 56, 86, 149, 188
HpyAV CCTTC 4 cut(s) 76, 106, 235, 374
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 7 cut(s) 290, 344, 424, 427, 472, 579, 619
HpyF3I CTNAG 2 cut(s) 499, 561
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 2 cut(s) 330, 384
Hsp92II CATG 5 cut(s) 172, 241, 355, 397, 412
HspAI GCGC 6 cut(s) 135, 137, 288, 330, 384, 611
KasI GGCGCC 2 cut(s) 329, 383
KroI GCCGGC 2 cut(s) 284, 565
KroNI GCCGGC 2 cut(s) 286, 567
Kzo9I GATC 3 cut(s) 375, 390, 448
LmnI GCTCC 3 cut(s) 25, 62, 158
Lsp1109I GCAGC 5 cut(s) 430, 439, 442, 445, 585
LweI GCATC 4 cut(s) 123, 303, 462, 598
MaeII ACGT 1 cut(s) 54
MalI GATC 3 cut(s) 377, 392, 450
MboI GATC 3 cut(s) 375, 390, 448
MboII GAAGA 2 cut(s) 208, 515
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 35, 624
Mly113I GGCGCC 2 cut(s) 330, 384
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 2 cut(s) 64, 127
MreI CGCCGGCG 1 cut(s) 284
MroNI GCCGGC 2 cut(s) 284, 565
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 6 cut(s) 35, 285, 297, 387, 495, 566
MspR9I CCNGG 7 cut(s) 35, 158, 268, 388, 453, 468, 495
MvaI CCWGG 4 cut(s) 158, 268, 453, 468
MvnI CGCG 5 cut(s) 137, 139, 183, 290, 338
MwoI GCNNNNNNNGC 7 cut(s) 290, 344, 424, 427, 472, 579, 619
NaeI GCCGGC 2 cut(s) 286, 567
NarI GGCGCC 2 cut(s) 330, 384
NciI CCSGG 3 cut(s) 35, 388, 495
NcoI CCATGG 2 cut(s) 237, 351
NdeII GATC 3 cut(s) 375, 390, 448
NgoMIV GCCGGC 2 cut(s) 284, 565
NlaIII CATG 5 cut(s) 172, 241, 355, 397, 412
NlaIV GGNNCC 8 cut(s) 27, 64, 79, 154, 264, 331, 385, 450
NmeAIII GCCGAG 1 cut(s) 306
PaeR7I CTCGAG 1 cut(s) 596
PagI TCATGA 1 cut(s) 408
PauI GCGCGC 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PdiI GCCGGC 2 cut(s) 286, 567
PfeI GAWTC 1 cut(s) 42
PleI GAGTC 1 cut(s) 207
PluTI GGCGCC 2 cut(s) 333, 387
PpsI GAGTC 1 cut(s) 207
PpuMI RGGWCCY 2 cut(s) 262, 592
PshAI GACNNNNGTC 1 cut(s) 590
Psp5II RGGWCCY 2 cut(s) 262, 592
Psp6I CCWGG 4 cut(s) 156, 266, 451, 466
PspGI CCWGG 4 cut(s) 156, 266, 451, 466
PspN4I GGNNCC 8 cut(s) 27, 64, 79, 154, 264, 331, 385, 450
PspPI GGNCC 7 cut(s) 78, 262, 322, 342, 370, 488, 592
PspPPI RGGWCCY 2 cut(s) 262, 592
PsuI RGATCY 1 cut(s) 448
PteI GCGCGC 1 cut(s) 135
Sau3AI GATC 3 cut(s) 375, 390, 448
Sau96I GGNCC 7 cut(s) 78, 262, 322, 342, 370, 488, 592
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 7 cut(s) 35, 158, 268, 388, 453, 468, 495
SduI GDGCHC 2 cut(s) 35, 624
SetI ASST 7 cut(s) 57, 197, 227, 249, 344, 512, 594
SfaNI GCATC 4 cut(s) 123, 303, 462, 598
SfoI GGCGCC 2 cut(s) 331, 385
Sfr274I CTCGAG 1 cut(s) 596
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 3 cut(s) 262, 342, 592
SlaI CTCGAG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
SspDI GGCGCC 2 cut(s) 329, 383
StyD4I CCNGG 7 cut(s) 33, 156, 266, 386, 451, 466, 493
StyI CCWWGG 4 cut(s) 129, 237, 351, 588
TaiI ACGT 1 cut(s) 57
TaqI TCGA 4 cut(s) 48, 228, 378, 597
TauI GCSGC 5 cut(s) 95, 293, 319, 350, 478
TfiI GAWTC 1 cut(s) 42
TscAI CASTG 1 cut(s) 624
TseI GCWGC 5 cut(s) 418, 427, 430, 458, 573
TspDTI ATGAA 2 cut(s) 94, 185
TspGWI ACGGA 1 cut(s) 505
TspRI CASTG 1 cut(s) 624
VpaK11BI GGWCC 3 cut(s) 262, 342, 592
XhoI CTCGAG 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.