Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0008749

Physical Location & Seq

Genomic Coordinates Forward (+)

2239728 .. 2246291

Gene Structure 6564 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG014100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2181 bp

ATGGGAGCGGTGCCGTCGACGCCGCGCTGGGGGAGTAGCTCCGCGGCGCGTCCGCTGGAAACGGCGGAGTATCTGATCGGGACCTTCGTCGGCGACGAGTCGTTTCCTTTGTCCTCTGATTACTGGCAGAAATTGCTCGACCTCCCTCTCAGTCTCCAATGGCCTCCTGACCGCGTTCAACAAGCTTGCGAGGCTCTTGCACAAAACAACTACAAAACCAGGCATCTCGCAAAGCTTCTGATCCATCTGACATGGTGTTTACAAGAAGCTATTTCAACTTCTGGTGGCCCTTCTCTTGTTTATGGGAAGGCTGTTAATGCAGTGTACATCTCATCTGTCTTTTTAAAGTACCTAATTGAAAATCCAAAAAGCGACAAGATTGAAGAGTTGTACTTATCTCTAGATGAAAGTGAGCCAATGCCAACTGATATTACAAGAGATCTAAACATTGAGGATTTTGTAATGTGTAGTGTGCTTACCTTTATTGGATCAATAGATGTAAGTCCTGGTACATACCTCCTTCATTTGGAACTGCTTAACTTCATGCTGATTGCAATGTCAACTCAGCTTCTTTCTGGGCCATCCCCAGGACCAGAAGATGTGAACCCATTTCTTGATGCAGCAATGTCTCAGGAAAGCTCGTTGGTTATCTCGGTTGTTCGTAGACTGCTACTCAATTACATCACTGGACCTTCGGTACCCCTGAATAGTGCATCTTATTCTATATTTTCTGAAGGAAGCCAGCCTGGTGTTATACAAAGAGTTAGTTCTGCAGCAGCAAATTTGATGTTATTACCATTCAACTTTCTCGTCAGTTCAAGTGGTGAAGGCTCAAGAAGTCTTTTGGCTGACTGCAGTCTCCATGTGCTACTTATTCTCACTCATTATCACAAATGTGTTGTGGGCAACGAGCCAATAACAGATACAAGTAATGACACCACTACTTCAAATTCACTTTTGAAAGGGTGTAGATATTTTTCTGACAACCCTTACTGCAAGGCCTTGGAACATGCAACAGATGTTGAATTTGATCGTGTAGATACTGAGGGTAATGCACATAGTGGTCCAGTTTTGAGGTTACCTTTTGCTTCCCTGTTCGATACCCTTGGAATGTGCTTGTCTGATGAGGCTGCTGCACTTTTGCTTTACTCTTTATTGCAAGGAAATGCCGACTTTCTGGAGTATGTCTTGGTGCGAACTGATTTGGATACGCTGTTGATGCCCATCCTGGAAGCGTTATATGATGCTCCAAAAAGGACATCTAATCAAATCTACATGTTGCTGATCATACTTCTCATACTTAGTCAGGATTCATCTTTTAATGCAAGCATTCACAAATTGATACTTCCTACTGTTCCTTGGTACAAAGAACGTCTCCTCCACCAGACGTCTCTTGGTTCATTAATGGTCATTACGTTGATAAGGACAGTGCAGTATAACCTATCTAAGTTGCGGGATGTTTATCTCCATACAACTTGTCTAGCAACTTTGGCAAACATGGCACCTCATGTCCACCGTTTGAGTGCATATGCATCCCAGAGATTGGTCAGCCTATTTGATATGCTCTCTCGGAAGTATAACAAACTGGCAGAGGTGCGTAATAATCAGATGACATTAGCCAAAGGCAACTCAATAGAAGACGATACTTCAACGGAGATGCATATATATACCGACTTCTTGAGACTTGTCCTTGAAATATTAAATGCAATTTTGACATATGCTCTGCCACGGAATCCTGAGGTTATATATGCGATAATGCACAGGCAGGAAGTATTTCAGCCTTTCAGGAATCATCCACGTTTCAATGAGCTGCTTGAAAACATTTATACAGTGTTAGATTTTTTCAATAGTCGTGTGGATGCACAAAGAACAGATGGAGAATGGTCAGTAGAAAAAGTACTTCAAGTCATAATCATTAATTGTAGATCATGGCGTGGTGAAGGGATGAAGATGTTCACCCAGTTGCGCTTCACATATGAACAAGAGAGTCATCCAGAGGAGTTCTTTATTCCATACTTGTGGCAGCTAGTACTATCCCGCTGTGGAATCAGTTTCAATCCTCGAGCCATAAATTTGTTTCCAATTGATCAACCTTCCGAGAAACAGAGTGATTATGAAGTGGCGTCTGACAATCATGTAAATGGAGAGGTGGCCAAGCATGCAGTCTTTCTTGATCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dymeclin

Protein Analysis

726

Amino Acids

81.67

Weight (kDa)

5.16

Isoelectric Point (pI)

45.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dymeclin	PF09742	1 - 676	4.9e-195	Dyggve-Melchior-Clausen syndrome protein
Hid1	PF12722	380 - 537	2.4e-09	High-temperature-induced dauer-formation protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008749)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04200 AT1G04200 AT1G04200
fragaria_vesca	FvH4_4g01230 FvH4_4g01230 FvH4_4g01230
malus_domestica	MD13G1199900.v1.1 MD16G1199900.v1.1
prunus_persica	Prupe.1G021300_v2.0.a1
pyrus_communis	pycom13g17340 pycom16g16850
rosa_chinensis	RchiOBHm_Chr4g0387781
rosa_laevigata	RLG00000010096
rosa_multiflora	Rmu_sc0008916.1_g000003 Rmu_sc0013958.1_g000003
rosa_roxburghii	Rroxscaffold_5G00334400
rosa_rugosa	Rorug03G0313100 Rorug03G0313200 Rorug03G0313300
rosa_samantha	Rh4AG018300 Rh4CG019500 Rh4DG014100
rosa_wichuraiana	Rw4G001200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1391
Acc65I	GGTACC	1 cut(s)	697
AccB1I	GGYRCC	3 cut(s)	10, 697, 1501
AccB7I	CCANNNNNTGG	1 cut(s)	1543
AccBSI	CCGCTC	1 cut(s)	8
AccI	GTMKAC	2 cut(s)	17, 664
AccII	CGCG	4 cut(s)	25, 44, 49, 174
AciI	CCGC	9 cut(s)	8, 23, 42, 44, 53, 65, 172, 1453, 2038
AclWI	GGATC	3 cut(s)	235, 496, 2168
AcoI	YGGCCR	1 cut(s)	2151
AcsI	RAATTY	4 cut(s)	781, 949, 1025, 2071
AcuI	CTGAAG	1 cut(s)	753
AcyI	GRCGYC	3 cut(s)	20, 1388, 2123
AdeI	CACNNNGTG	1 cut(s)	1061
AfaI	GTAC	8 cut(s)	326, 350, 392, 511, 699, 1364, 1899, 2031
AfiI	CCNNNNNNNGG	4 cut(s)	29, 526, 587, 1543
AflIII	ACRYGT	1 cut(s)	1275
AjnI	CCWGG	5 cut(s)	218, 505, 586, 745, 1227
AleI	CACNNNNGTG	1 cut(s)	894
AloI	GAACNNNNNNTCC	2 cut(s)	1362, 1394
AluBI	AGCT	8 cut(s)	39, 185, 235, 269, 568, 639, 1810, 2026
AluI	AGCT	8 cut(s)	39, 185, 235, 269, 568, 639, 1810, 2026
Alw26I	GTCTC	6 cut(s)	158, 633, 863, 1379, 1395, 1675
AlwI	GGATC	3 cut(s)	235, 496, 2168
Ama87I	CYCGRG	1 cut(s)	2061
AoxI	GGCC	5 cut(s)	161, 286, 578, 999, 2151
ApeKI	GCWGC	7 cut(s)	620, 773, 776, 1130, 1133, 1810, 2023
ApoI	RAATTY	4 cut(s)	781, 949, 1025, 2071
AseI	ATTAAT	2 cut(s)	1403, 1917
Asp700I	GAANNNNTTC	2 cut(s)	1773, 1952
Asp718I	GGTACC	1 cut(s)	697
AspLEI	GCGC	3 cut(s)	27, 49, 1968
AspS9I	GGNCC	6 cut(s)	81, 287, 578, 590, 689, 1064
AsuHPI	GGTGA	3 cut(s)	836, 1948, 1949
AvaI	CYCGRG	1 cut(s)	2061
AvaII	GGWCC	4 cut(s)	81, 590, 689, 1064
AxyI	CCTNAGG	1 cut(s)	1737
BalI	TGGCCA	1 cut(s)	2153
BanI	GGYRCC	3 cut(s)	10, 697, 1501
BbsI	GAAGAC	1 cut(s)	1644
BbvI	GCAGC	7 cut(s)	632, 785, 788, 1117, 1120, 1797, 2035
BccI	CCATC	4 cut(s)	252, 589, 1232, 1868
BceAI	ACGGC	1 cut(s)	78
BcgI	CGANNNNNNTGC	2 cut(s)	179, 213
BciT130I	CCWGG	5 cut(s)	220, 507, 588, 747, 1229
BciVI	GTATCC	1 cut(s)	1201
BclI	TGATCA	2 cut(s)	1284, 2086
BcoDI	GTCTC	6 cut(s)	158, 633, 863, 1379, 1395, 1675
BfaI	CTAG	3 cut(s)	401, 1481, 2027
BfmI	CTRYAG	2 cut(s)	771, 853
BfuI	GTATCC	1 cut(s)	1201
BglII	AGATCT	1 cut(s)	439
BisI	GCNGC	9 cut(s)	23, 45, 621, 774, 777, 1131, 1134, 1811, 2024
BlsI	GCNGC	9 cut(s)	24, 46, 622, 775, 778, 1132, 1135, 1812, 2025
BmcAI	AGTACT	2 cut(s)	1899, 2031
Bme1390I	CCNGG	5 cut(s)	220, 507, 588, 747, 1229
Bme18I	GGWCC	4 cut(s)	81, 590, 689, 1064
BmeT110I	CYCGRG	1 cut(s)	2061
BmgT120I	GGNCC	6 cut(s)	81, 287, 578, 590, 689, 1064
BmiI	GGNNCC	4 cut(s)	12, 82, 699, 1503
BmrFI	CCNGG	5 cut(s)	220, 507, 588, 747, 1229
BmrI	ACTGGG	1 cut(s)	1954
BmsI	GCATC	8 cut(s)	232, 607, 722, 1209, 1234, 1541, 1647, 1849
BmuI	ACTGGG	1 cut(s)	1954
BoxI	GACNNNNGTC	2 cut(s)	86, 855
BpiI	GAAGAC	1 cut(s)	1644
BpmI	CTGGAG	1 cut(s)	1199
BpuEI	CTTGAG	2 cut(s)	817, 1699
BsaBI	GATNNNNATC	2 cut(s)	1223, 1461
BsaHI	GRCGYC	3 cut(s)	20, 1388, 2123
BsaJI	CCNNGG	6 cut(s)	42, 586, 1002, 1105, 1358, 1727
BsaXI	ACNNNNNCTCC	2 cut(s)	1362, 1392
Bsc4I	CCNNNNNNNGG	4 cut(s)	29, 526, 587, 1543
Bse1I	ACTGG	5 cut(s)	128, 691, 1067, 1590, 1960
Bse21I	CCTNAGG	1 cut(s)	1737
Bse3DI	GCAATG	2 cut(s)	561, 630
Bse8I	GATNNNNATC	2 cut(s)	1223, 1461
BseBI	CCWGG	5 cut(s)	220, 507, 588, 747, 1229
BseDI	CCNNGG	6 cut(s)	42, 586, 1002, 1105, 1358, 1727
BseGI	GGATG	8 cut(s)	581, 1224, 1462, 1532, 1792, 1864, 1950, 1990
BseJI	GATNNNNATC	2 cut(s)	1223, 1461
BseLI	CCNNNNNNNGG	4 cut(s)	29, 526, 587, 1543
BseMI	GCAATG	2 cut(s)	561, 630
BseMII	CTCAG	5 cut(s)	163, 578, 644, 1035, 1728
BseNI	ACTGG	5 cut(s)	128, 691, 1067, 1590, 1960
BseRI	GAGGAG	2 cut(s)	1367, 2012
BseXI	GCAGC	7 cut(s)	632, 785, 788, 1117, 1120, 1797, 2035
BseYI	CCCAGC	1 cut(s)	27
BsgI	GTGCAG	2 cut(s)	1119, 1451
Bsh1236I	CGCG	4 cut(s)	25, 44, 49, 174
BshFI	GGCC	5 cut(s)	163, 288, 580, 1001, 2153
BshNI	GGYRCC	3 cut(s)	10, 697, 1501
BsiHKCI	CYCGRG	1 cut(s)	2061
BslFI	GGGAC	1 cut(s)	94
BslI	CCNNNNNNNGG	4 cut(s)	29, 526, 587, 1543
BsmAI	GTCTC	6 cut(s)	158, 633, 863, 1379, 1395, 1675
BsmBI	CGTCTC	2 cut(s)	1379, 1395
BsmFI	GGGAC	1 cut(s)	94
BsmI	GAATGC	1 cut(s)	1329
BsnI	GGCC	5 cut(s)	163, 288, 580, 1001, 2153
BsoBI	CYCGRG	1 cut(s)	2061
Bsp1407I	TGTACA	1 cut(s)	324
Bsp143I	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
BspACI	CCGC	9 cut(s)	8, 23, 42, 44, 53, 65, 172, 1453, 2038
BspANI	GGCC	5 cut(s)	163, 288, 580, 1001, 2153
BspCNI	CTCAG	5 cut(s)	162, 577, 643, 1036, 1729
BspFNI	CGCG	4 cut(s)	25, 44, 49, 174
BspLI	GGNNCC	4 cut(s)	12, 82, 699, 1503
BspMAI	CTGCAG	2 cut(s)	775, 857
BspPI	GGATC	3 cut(s)	235, 496, 2168
BspT107I	GGYRCC	3 cut(s)	10, 697, 1501
BsrBI	CCGCTC	1 cut(s)	8
BsrDI	GCAATG	2 cut(s)	561, 630
BsrGI	TGTACA	1 cut(s)	324
BsrI	ACTGG	5 cut(s)	128, 691, 1067, 1590, 1960
BssECI	CCNNGG	6 cut(s)	42, 586, 1002, 1105, 1358, 1727
BssMI	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
BssNI	GRCGYC	3 cut(s)	20, 1388, 2123
BssT1I	CCWWGG	3 cut(s)	1002, 1105, 1358
Bst2UI	CCWGG	5 cut(s)	220, 507, 588, 747, 1229
Bst4CI	ACNGT	4 cut(s)	1354, 1429, 1517, 1831
Bst6I	CTCTTC	1 cut(s)	378
BstACI	GRCGYC	3 cut(s)	20, 1388, 2123
BstAPI	GCANNNNNTGC	1 cut(s)	133
BstAUI	TGTACA	1 cut(s)	324
BstC8I	GCNNGC	4 cut(s)	187, 743, 1327, 2160
BstDEI	CTNAG	7 cut(s)	149, 564, 630, 1044, 1301, 1446, 1737
BstDSI	CCRYGG	2 cut(s)	42, 1727
BstEII	GGTNACC	1 cut(s)	1077
BstF5I	GGATG	8 cut(s)	581, 1224, 1462, 1532, 1792, 1864, 1950, 1990
BstFNI	CGCG	4 cut(s)	25, 44, 49, 174
BstHHI	GCGC	3 cut(s)	27, 49, 1968
BstKTI	GATC	9 cut(s)	78, 243, 442, 491, 1033, 1287, 1928, 2089, 2176
BstMAI	GTCTC	6 cut(s)	158, 633, 863, 1379, 1395, 1675
BstMBI	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
BstMWI	GCNNNNNNNGC	8 cut(s)	19, 133, 191, 317, 1219, 1490, 1499, 2159
BstNI	CCWGG	5 cut(s)	220, 507, 588, 747, 1229
BstNSI	RCATGY	3 cut(s)	1013, 1279, 2162
BstPAI	GACNNNNGTC	2 cut(s)	86, 855
BstPI	GGTNACC	1 cut(s)	1077
BstSCI	CCNGG	5 cut(s)	218, 505, 586, 745, 1227
BstSFI	CTRYAG	2 cut(s)	771, 853
BstUI	CGCG	4 cut(s)	25, 44, 49, 174
BstV1I	GCAGC	7 cut(s)	632, 785, 788, 1117, 1120, 1797, 2035
BstV2I	GAAGAC	1 cut(s)	1644
BstX2I	RGATCY	1 cut(s)	439
BstXI	CCANNNNNNTGG	1 cut(s)	2019
BstYI	RGATCY	1 cut(s)	439
Bsu36I	CCTNAGG	1 cut(s)	1737
BsuI	GTATCC	1 cut(s)	1201
BsuRI	GGCC	5 cut(s)	163, 288, 580, 1001, 2153
BtgI	CCRYGG	2 cut(s)	42, 1727
BtsCI	GGATG	8 cut(s)	581, 1224, 1462, 1532, 1792, 1864, 1950, 1990
BtsI	GCAGTG	1 cut(s)	327
BtsIMutI	CAGTG	4 cut(s)	327, 684, 1434, 1836
Cac8I	GCNNGC	4 cut(s)	187, 743, 1327, 2160
CfoI	GCGC	3 cut(s)	27, 49, 1968
Cfr13I	GGNCC	6 cut(s)	81, 287, 578, 590, 689, 1064
Cfr42I	CCGCGG	1 cut(s)	45
CseI	GACGC	3 cut(s)	28, 38, 2112
Csp6I	GTAC	8 cut(s)	325, 349, 391, 510, 698, 1363, 1898, 2030
CviQI	GTAC	8 cut(s)	325, 349, 391, 510, 698, 1363, 1898, 2030
DdeI	CTNAG	7 cut(s)	149, 564, 630, 1044, 1301, 1446, 1737
DpnI	GATC	9 cut(s)	77, 242, 441, 490, 1032, 1286, 1927, 2088, 2175
DpnII	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
DraI	TTTAAA	1 cut(s)	345
DraIII	CACNNNGTG	1 cut(s)	1061
EaeI	YGGCCR	1 cut(s)	2151
Eam1104I	CTCTTC	1 cut(s)	378
EarI	CTCTTC	1 cut(s)	378
EciI	GGCGGA	1 cut(s)	80
Eco130I	CCWWGG	3 cut(s)	1002, 1105, 1358
Eco147I	AGGCCT	1 cut(s)	1001
Eco47I	GGWCC	4 cut(s)	81, 590, 689, 1064
Eco57I	CTGAAG	1 cut(s)	753
Eco81I	CCTNAGG	1 cut(s)	1737
Eco88I	CYCGRG	1 cut(s)	2061
Eco91I	GGTNACC	1 cut(s)	1077
EcoO109I	RGGNCCY	1 cut(s)	81
EcoO65I	GGTNACC	1 cut(s)	1077
EcoRII	CCWGG	5 cut(s)	218, 505, 586, 745, 1227
EcoT14I	CCWWGG	3 cut(s)	1002, 1105, 1358
EcoT22I	ATGCAT	2 cut(s)	1534, 1662
ErhI	CCWWGG	3 cut(s)	1002, 1105, 1358
Esp3I	CGTCTC	2 cut(s)	1379, 1395
FaqI	GGGAC	1 cut(s)	94
FauI	CCCGC	2 cut(s)	1446, 2045
FauNDI	CATATG	3 cut(s)	1528, 1717, 1975
FbaI	TGATCA	2 cut(s)	1284, 2086
FblI	GTMKAC	2 cut(s)	17, 664
Fnu4HI	GCNGC	9 cut(s)	23, 45, 621, 774, 777, 1131, 1134, 1811, 2024
FokI	GGATG	8 cut(s)	568, 1211, 1469, 1519, 1779, 1871, 1957, 1977
Fsp4HI	GCNGC	9 cut(s)	23, 45, 621, 774, 777, 1131, 1134, 1811, 2024
FspBI	CTAG	3 cut(s)	401, 1481, 2027
GlaI	GCGC	3 cut(s)	26, 48, 1967
GluI	GCNGC	9 cut(s)	23, 45, 621, 774, 777, 1131, 1134, 1811, 2024
GsaI	CCCAGC	1 cut(s)	31
GsuI	CTGGAG	1 cut(s)	1199
HaeIII	GGCC	5 cut(s)	163, 288, 580, 1001, 2153
HgaI	GACGC	3 cut(s)	28, 38, 2112
HhaI	GCGC	3 cut(s)	27, 49, 1968
Hin1I	GRCGYC	3 cut(s)	20, 1388, 2123
Hin6I	GCGC	3 cut(s)	25, 47, 1966
HinP1I	GCGC	3 cut(s)	25, 47, 1966
HincII	GTYRAC	2 cut(s)	18, 561
HindII	GTYRAC	2 cut(s)	18, 561
HindIII	AAGCTT	2 cut(s)	183, 233
HinfI	GANTC	6 cut(s)	98, 1310, 1732, 1789, 1987, 2046
HphI	GGTGA	3 cut(s)	836, 1948, 1949
Hpy166II	GTNNAC	8 cut(s)	18, 260, 325, 561, 604, 665, 1513, 1956
Hpy8I	GTNNAC	8 cut(s)	18, 260, 325, 561, 604, 665, 1513, 1956
Hpy99I	CGWCG	4 cut(s)	19, 22, 92, 98
HpyAV	CCTTC	9 cut(s)	94, 300, 301, 530, 702, 728, 821, 1934, 2103
HpyCH4III	ACNGT	4 cut(s)	1354, 1429, 1517, 1831
HpyCH4IV	ACGT	4 cut(s)	1372, 1388, 1415, 1798
HpyF10VI	GCNNNNNNNGC	8 cut(s)	19, 133, 191, 317, 1219, 1490, 1499, 2159
HpyF3I	CTNAG	7 cut(s)	149, 564, 630, 1044, 1301, 1446, 1737
HpySE526I	ACGT	4 cut(s)	1372, 1388, 1415, 1798
Hsp92I	GRCGYC	3 cut(s)	20, 1388, 2123
HspAI	GCGC	3 cut(s)	25, 47, 1966
KpnI	GGTACC	1 cut(s)	701
Ksp22I	TGATCA	2 cut(s)	1284, 2086
KspI	CCGCGG	1 cut(s)	45
Kzo9I	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
LmnI	GCTCC	3 cut(s)	5, 44, 1252
Lsp1109I	GCAGC	7 cut(s)	632, 785, 788, 1117, 1120, 1797, 2035
LweI	GCATC	8 cut(s)	232, 607, 722, 1209, 1234, 1541, 1647, 1849
MaeI	CTAG	3 cut(s)	401, 1481, 2027
MaeII	ACGT	4 cut(s)	1372, 1388, 1415, 1798
MaeIII	GTNAC	1 cut(s)	1077
MalI	GATC	9 cut(s)	77, 242, 441, 490, 1032, 1286, 1927, 2088, 2175
MbiI	CCGCTC	1 cut(s)	8
MboI	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
MboII	GAAGA	4 cut(s)	395, 608, 1649, 1960
MfeI	CAATTG	1 cut(s)	2082
MflI	RGATCY	1 cut(s)	439
MlsI	TGGCCA	1 cut(s)	2153
MluNI	TGGCCA	1 cut(s)	2153
MlyI	GAGTC	2 cut(s)	107, 1996
Mox20I	TGGCCA	1 cut(s)	2153
Mph1103I	ATGCAT	2 cut(s)	1534, 1662
MroXI	GAANNNNTTC	2 cut(s)	1773, 1952
MscI	TGGCCA	1 cut(s)	2153
MseI	TTAA	7 cut(s)	315, 344, 537, 1320, 1403, 1700, 1917
MslI	CAYNNNNRTG	3 cut(s)	894, 2017, 2139
Msp20I	TGGCCA	1 cut(s)	2153
MspA1I	CMGCKG	3 cut(s)	44, 55, 2040
MspR9I	CCNGG	5 cut(s)	220, 507, 588, 747, 1229
MunI	CAATTG	1 cut(s)	2082
Mva1269I	GAATGC	1 cut(s)	1329
MvaI	CCWGG	5 cut(s)	220, 507, 588, 747, 1229
MvnI	CGCG	4 cut(s)	25, 44, 49, 174
MwoI	GCNNNNNNNGC	8 cut(s)	19, 133, 191, 317, 1219, 1490, 1499, 2159
NdeI	CATATG	3 cut(s)	1528, 1717, 1975
NdeII	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
NlaIV	GGNNCC	4 cut(s)	12, 82, 699, 1503
NsiI	ATGCAT	2 cut(s)	1534, 1662
NspI	RCATGY	3 cut(s)	1013, 1279, 2162
OliI	CACNNNNGTG	1 cut(s)	894
PaeI	GCATGC	1 cut(s)	2162
PaeR7I	CTCGAG	1 cut(s)	2061
PceI	AGGCCT	1 cut(s)	1001
PciI	ACATGT	1 cut(s)	1275
PcsI	WCGNNNNNNNCGW	2 cut(s)	84, 93
PctI	GAATGC	1 cut(s)	1329
PdmI	GAANNNNTTC	2 cut(s)	1773, 1952
PfeI	GAWTC	4 cut(s)	1310, 1732, 1789, 2046
PflMI	CCANNNNNTGG	1 cut(s)	1543
PfoI	TCCNGGA	1 cut(s)	1227
PkrI	GCNGC	9 cut(s)	24, 46, 622, 775, 778, 1132, 1135, 1812, 2025
PleI	GAGTC	2 cut(s)	106, 1995
PpsI	GAGTC	2 cut(s)	106, 1995
PpuMI	RGGWCCY	1 cut(s)	81
PscI	ACATGT	1 cut(s)	1275
PshAI	GACNNNNGTC	2 cut(s)	86, 855
PshBI	ATTAAT	2 cut(s)	1403, 1917
Psp5II	RGGWCCY	1 cut(s)	81
Psp6I	CCWGG	5 cut(s)	218, 505, 586, 745, 1227
PspEI	GGTNACC	1 cut(s)	1077
PspFI	CCCAGC	1 cut(s)	27
PspGI	CCWGG	5 cut(s)	218, 505, 586, 745, 1227
PspN4I	GGNNCC	4 cut(s)	12, 82, 699, 1503
PspPI	GGNCC	6 cut(s)	81, 287, 578, 590, 689, 1064
PspPPI	RGGWCCY	1 cut(s)	81
PspXI	VCTCGAGB	1 cut(s)	2061
PstI	CTGCAG	2 cut(s)	775, 857
PsuI	RGATCY	1 cut(s)	439
RsaI	GTAC	8 cut(s)	326, 350, 392, 511, 699, 1364, 1899, 2031
RsaNI	GTAC	8 cut(s)	325, 349, 391, 510, 698, 1363, 1898, 2030
RseI	CAYNNNNRTG	3 cut(s)	894, 2017, 2139
SacII	CCGCGG	1 cut(s)	45
SalI	GTCGAC	1 cut(s)	16
SaqAI	TTAA	7 cut(s)	315, 344, 537, 1320, 1403, 1700, 1917
SatI	GCNGC	9 cut(s)	23, 45, 621, 774, 777, 1131, 1134, 1811, 2024
Sau3AI	GATC	9 cut(s)	75, 240, 439, 488, 1030, 1284, 1925, 2086, 2173
Sau96I	GGNCC	6 cut(s)	81, 287, 578, 590, 689, 1064
ScaI	AGTACT	2 cut(s)	1899, 2031
SchI	GAGTC	2 cut(s)	107, 1996
ScrFI	CCNGG	5 cut(s)	220, 507, 588, 747, 1229
SfaNI	GCATC	8 cut(s)	232, 607, 722, 1209, 1234, 1541, 1647, 1849
SfcI	CTRYAG	2 cut(s)	771, 853
Sfr274I	CTCGAG	1 cut(s)	2061
Sfr303I	CCGCGG	1 cut(s)	45
SgrBI	CCGCGG	1 cut(s)	45
SgrDI	CGTCGACG	1 cut(s)	16
SinI	GGWCC	4 cut(s)	81, 590, 689, 1064
SlaI	CTCGAG	1 cut(s)	2061
SmiMI	CAYNNNNRTG	3 cut(s)	894, 2017, 2139
SmlI	CTYRAG	3 cut(s)	832, 1678, 2061
SmoI	CTYRAG	3 cut(s)	832, 1678, 2061
SphI	GCATGC	1 cut(s)	2162
SseBI	AGGCCT	1 cut(s)	1001
SsiI	CCGC	9 cut(s)	8, 23, 42, 44, 53, 65, 172, 1453, 2038
SspI	AATATT	1 cut(s)	1698
SspMI	CTAG	3 cut(s)	401, 1481, 2027
StuI	AGGCCT	1 cut(s)	1001
StyD4I	CCNGG	5 cut(s)	218, 505, 586, 745, 1227
StyI	CCWWGG	3 cut(s)	1002, 1105, 1358
TaaI	ACNGT	4 cut(s)	1354, 1429, 1517, 1831
TaiI	ACGT	4 cut(s)	1375, 1391, 1418, 1801
TaqI	TCGA	4 cut(s)	17, 138, 1098, 2062
TatI	WGTACW	4 cut(s)	324, 390, 1897, 2029
TauI	GCSGC	2 cut(s)	25, 47
TfiI	GAWTC	4 cut(s)	1310, 1732, 1789, 2046
Tru1I	TTAA	7 cut(s)	315, 344, 537, 1320, 1403, 1700, 1917
Tru9I	TTAA	7 cut(s)	315, 344, 537, 1320, 1403, 1700, 1917
TscAI	CASTG	4 cut(s)	327, 691, 1434, 1836
TseI	GCWGC	7 cut(s)	620, 773, 776, 1130, 1133, 1810, 2023
TspDTI	ATGAA	8 cut(s)	420, 512, 532, 1302, 1389, 1961, 1992, 2130
TspGWI	ACGGA	2 cut(s)	1667, 1744
TspRI	CASTG	4 cut(s)	327, 691, 1434, 1836
Van91I	CCANNNNNTGG	1 cut(s)	1543
VpaK11BI	GGWCC	4 cut(s)	81, 590, 689, 1064
VspI	ATTAAT	2 cut(s)	1403, 1917
XapI	RAATTY	4 cut(s)	781, 949, 1025, 2071
XbaI	TCTAGA	1 cut(s)	400
XceI	RCATGY	3 cut(s)	1013, 1279, 2162
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1391
XhoI	CTCGAG	1 cut(s)	2061
XmiI	GTMKAC	2 cut(s)	17, 664
XmnI	GAANNNNTTC	2 cut(s)	1773, 1952
XspI	CTAG	3 cut(s)	401, 1481, 2027
ZraI	GACGTC	1 cut(s)	1389
ZrmI	AGTACT	2 cut(s)	1899, 2031
Zsp2I	ATGCAT	2 cut(s)	1534, 1662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.