Rorug04G0035500

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015221
Physical Location & Seq
Forward (+)
5290149 .. 5290859
711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0035500.1

Sequence Viewer

Length: 711 bp
ATGGAACCACAAACCCAGCAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTGTAGGCGGAGCAGGTGGACTCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTTTACAATAAGGGAGTTAGGTCCCCAACTGTAGAGCAGGTTCAGAGGATCTATCTCCAGCTAAAATGGTATGGCAAGATCGAGTTCAAGAACGTCTATTATTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCAGAAGAAGAGGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGACAATGTTGCCAATTCATCTGCACCTTGTTCTGCTTCTTCTGCTGGTGTGATGATTGCTTTTAACGGGCAGATGGGGAACTATGGCGGTTATGGATCCATGAACATGGAGAAGAGTGCTAGGGATTGTTCAATCTCAGCTGGAGGGGGAACTAGTTCTACTTTCTTTGACATGAATGTAGAACAAACTTTCATGGAACAAAGAGGAGAAGATCACCAGGAAATTGAAACCCTTTCACTGTTTCCCGTGCATGGTGAGGACATCTTTGGCAACATGAAGACTACTTCCGATGGAGGTGGCGGTCACGGTGGTGGCTCTCACACTTCCCTTGAGCTCATCCTTTCCTGCGAGGGCAAAGCTGGAAAAAGTTTCCGGAGCCGCTGA

Protein Analysis

236

Amino Acids

25.97

Weight (kDa)

8.31

Isoelectric Point (pI)

53.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 27 - 89 1.1e-09 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57420
fragaria_vesca FvH4_4g08200
malus_domestica MD13G1253600.v1.1
prunus_persica Prupe.1G085900_v2.0.a1
pyrus_communis pycom13g22450
rosa_chinensis RchiOBHm_Chr4g0402441
rosa_laevigata RLG00000009083
rosa_multiflora Rmu_ssc0000330.1_g000026
rosa_roxburghii Rroxscaffold_5G00347140
rosa_rugosa Rorug04G0035400 Rorug04G0035500
rosa_samantha Rh4AG112800 Rh4BG105300 Rh4CG119600 Rh4DG104600
rosa_wichuraiana Rw4G009040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 78
Acc36I ACCTGC 2 cut(s) 78, 160
AccIII TCCGGA 1 cut(s) 699
AciI CCGC 4 cut(s) 82, 414, 627, 706
AclWI GGATC 3 cut(s) 188, 417, 430
AfiI CCNNNNNNNGG 1 cut(s) 578
AgsI TTSAA 3 cut(s) 220, 459, 554
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 1 cut(s) 543
AleI CACNNNNGTG 1 cut(s) 636
AluBI AGCT 5 cut(s) 130, 193, 467, 661, 686
AluI AGCT 5 cut(s) 130, 193, 467, 661, 686
Alw21I GWGCWC 1 cut(s) 663
AlwI GGATC 3 cut(s) 188, 417, 430
Ama87I CYCGRG 1 cut(s) 255
Aor13HI TCCGGA 1 cut(s) 699
ApeKI GCWGC 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 481
AspS9I GGNCC 2 cut(s) 153, 276
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 335, 533, 593
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 2 cut(s) 153, 276
BaeGI GKGCMC 1 cut(s) 300
BamHI GGATCC 1 cut(s) 422
BanII GRGCYC 2 cut(s) 256, 663
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 1 cut(s) 663
BbvI GCAGC 1 cut(s) 62
BccI CCATC 3 cut(s) 26, 394, 611
BciT130I CCWGG 1 cut(s) 545
BcnI CCSGG 1 cut(s) 55
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 2 cut(s) 447, 480
BfmI CTRYAG 2 cut(s) 102, 162
BfuAI ACCTGC 2 cut(s) 78, 160
BisI GCNGC 2 cut(s) 51, 706
BlsI GCNGC 2 cut(s) 52, 707
Bme1390I CCNGG 2 cut(s) 55, 545
Bme18I GGWCC 2 cut(s) 153, 276
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 153, 276
BmiI GGNNCC 4 cut(s) 6, 155, 424, 704
BmrFI CCNGG 2 cut(s) 55, 545
BpiI GAAGAC 1 cut(s) 611
BpmI CTGGAG 2 cut(s) 173, 489
BpuEI CTTGAG 2 cut(s) 107, 677
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 699
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse8I GATNNNNATC 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 699
BseBI CCWGG 1 cut(s) 545
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 3 cut(s) 37, 292, 663
BseJI GATNNNNATC 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMII CTCAG 1 cut(s) 477
BseRI GAGGAG 1 cut(s) 546
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 663
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 2 cut(s) 54, 700
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 578
BsmFI GGGAC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 3 cut(s) 256, 300, 663
Bsp13I TCCGGA 1 cut(s) 699
Bsp143I GATC 6 cut(s) 106, 180, 210, 308, 422, 538
BspACI CCGC 4 cut(s) 82, 414, 627, 706
BspCNI CTCAG 1 cut(s) 476
BspEI TCCGGA 1 cut(s) 699
BspLI GGNNCC 4 cut(s) 6, 155, 424, 704
BspMI ACCTGC 2 cut(s) 78, 160
BspPI GGATC 3 cut(s) 188, 417, 430
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 6 cut(s) 106, 180, 210, 308, 422, 538
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 3 cut(s) 163, 567, 635
Bst6I CTCTTC 2 cut(s) 266, 434
BstDEI CTNAG 1 cut(s) 463
BstF5I GGATG 3 cut(s) 37, 292, 663
BstKTI GATC 6 cut(s) 109, 183, 213, 311, 425, 541
BstMBI GATC 6 cut(s) 106, 180, 210, 308, 422, 538
BstMWI GCNNNNNNNGC 2 cut(s) 79, 368
BstNI CCWGG 1 cut(s) 545
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 53, 543
BstSFI CTRYAG 2 cut(s) 102, 162
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 2 cut(s) 180, 422
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 2 cut(s) 180, 422
BtsCI GGATG 3 cut(s) 37, 292, 663
BtsIMutI CAGTG 1 cut(s) 563
BveI ACCTGC 2 cut(s) 78, 160
Cfr13I GGNCC 2 cut(s) 153, 276
CviAII CATG 9 cut(s) 70, 293, 301, 427, 433, 499, 520, 578, 601
DdeI CTNAG 1 cut(s) 463
DpnI GATC 6 cut(s) 108, 182, 212, 310, 424, 540
DpnII GATC 6 cut(s) 106, 180, 210, 308, 422, 538
Eam1104I CTCTTC 2 cut(s) 266, 434
EarI CTCTTC 2 cut(s) 266, 434
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 661
Eco24I GRGCYC 2 cut(s) 256, 663
Eco47I GGWCC 2 cut(s) 153, 276
Eco53kI GAGCTC 1 cut(s) 661
Eco88I CYCGRG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 661
EcoO109I RGGNCCY 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 543
EcoT38I GRGCYC 2 cut(s) 256, 663
FaeI CATG 9 cut(s) 73, 296, 304, 430, 436, 502, 523, 581, 604
FaqI GGGAC 1 cut(s) 139
FatI CATG 9 cut(s) 69, 292, 300, 426, 432, 498, 519, 577, 600
Fnu4HI GCNGC 2 cut(s) 51, 706
FokI GGATG 3 cut(s) 44, 299, 650
FriOI GRGCYC 2 cut(s) 256, 663
Fsp4HI GCNGC 2 cut(s) 51, 706
FspBI CTAG 2 cut(s) 447, 480
GluI GCNGC 2 cut(s) 51, 706
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 2 cut(s) 173, 489
HapII CCGG 2 cut(s) 54, 700
Hin1II CATG 9 cut(s) 73, 296, 304, 430, 436, 502, 523, 581, 604
HinfI GANTC 2 cut(s) 94, 117
HpaII CCGG 2 cut(s) 54, 700
HphI GGTGA 3 cut(s) 335, 533, 593
Hpy166II GTNNAC 3 cut(s) 93, 244, 279
Hpy188I TCNGA 4 cut(s) 111, 177, 286, 616
Hpy188III TCNNGA 4 cut(s) 220, 241, 257, 700
Hpy8I GTNNAC 3 cut(s) 93, 244, 279
HpyCH4III ACNGT 3 cut(s) 163, 567, 635
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 304, 350, 577
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 368
HpyF3I CTNAG 1 cut(s) 463
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 9 cut(s) 73, 296, 304, 430, 436, 502, 523, 581, 604
Kpn2I TCCGGA 1 cut(s) 699
Kzo9I GATC 6 cut(s) 106, 180, 210, 308, 422, 538
LmnI GCTCC 3 cut(s) 84, 127, 702
Lsp1109I GCAGC 1 cut(s) 62
MaeI CTAG 2 cut(s) 447, 480
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 629
MalI GATC 6 cut(s) 108, 182, 212, 310, 424, 540
MboI GATC 6 cut(s) 106, 180, 210, 308, 422, 538
MboII GAAGA 6 cut(s) 280, 283, 357, 451, 548, 616
MflI RGATCY 2 cut(s) 180, 422
MhlI GDGCHC 3 cut(s) 256, 300, 663
MluCI AATT 2 cut(s) 340, 549
MlyI GAGTC 1 cut(s) 88
MroI TCCGGA 1 cut(s) 699
MroXI GAANNNNTTC 1 cut(s) 481
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 2 cut(s) 431, 636
MspA1I CMGCKG 2 cut(s) 467, 708
MspI CCGG 2 cut(s) 54, 700
MspR9I CCNGG 2 cut(s) 55, 545
MvaI CCWGG 1 cut(s) 545
MwoI GCNNNNNNNGC 2 cut(s) 79, 368
NciI CCSGG 1 cut(s) 55
NdeII GATC 6 cut(s) 106, 180, 210, 308, 422, 538
NlaIII CATG 9 cut(s) 73, 296, 304, 430, 436, 502, 523, 581, 604
NlaIV GGNNCC 4 cut(s) 6, 155, 424, 704
NmuCI GTSAC 1 cut(s) 629
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 636
PaqCI CACCTGC 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 481
PfeI GAWTC 1 cut(s) 117
PkrI GCNGC 2 cut(s) 52, 707
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 1 cut(s) 543
PspN4I GGNNCC 4 cut(s) 6, 155, 424, 704
PspPI GGNCC 2 cut(s) 153, 276
PspPPI RGGWCCY 1 cut(s) 153
PsrI GAACNNNNNNTAC 4 cut(s) 156, 188, 469, 501
PsuI RGATCY 2 cut(s) 180, 422
PvuII CAGCTG 1 cut(s) 467
RseI CAYNNNNRTG 2 cut(s) 431, 636
SacI GAGCTC 1 cut(s) 663
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 2 cut(s) 51, 706
Sau3AI GATC 6 cut(s) 106, 180, 210, 308, 422, 538
Sau96I GGNCC 2 cut(s) 153, 276
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 55, 545
SduI GDGCHC 3 cut(s) 256, 300, 663
SfcI CTRYAG 2 cut(s) 102, 162
SinI GGWCC 2 cut(s) 153, 276
SmiMI CAYNNNNRTG 2 cut(s) 431, 636
SmlI CTYRAG 2 cut(s) 122, 656
SmoI CTYRAG 2 cut(s) 122, 656
SpeI ACTAGT 1 cut(s) 479
Sse9I AATT 2 cut(s) 340, 549
SsiI CCGC 4 cut(s) 82, 414, 627, 706
SspMI CTAG 2 cut(s) 447, 480
SstI GAGCTC 1 cut(s) 663
StyD4I CCNGG 2 cut(s) 53, 543
TaaI ACNGT 3 cut(s) 163, 567, 635
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 213
TasI AATT 2 cut(s) 340, 549
TauI GCSGC 1 cut(s) 708
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 629
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 629
TspDTI ATGAA 6 cut(s) 281, 333, 443, 508, 515, 617
TspRI CASTG 1 cut(s) 570
VpaK11BI GGWCC 2 cut(s) 153, 276
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 481
XspI CTAG 2 cut(s) 447, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.