Rorug04G0070900

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007035
Physical Location & Seq
Forward (+)
10924220 .. 10926795
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0070900.1

Sequence Viewer

Length: 423 bp
ATGTCGAAGCGAGGACGCGGAGGATCGGCTGGGAACAAGTTCAGGATGTCACTGGGTCTACCGGTGGCGGCGACGGTGAACTGTGCTGACAATACGGGGGCGAAGAACCTGTACATCATCTCAGTGAAGGGAATCAAGGGTCGTCTGAATCGGTTGCCGTCTGCTTGCGTCGGTGACATGGTCATGGCCACTGTCAAGAAGGGTAAGCCTGATTTGAGGAAGAAGGTCCTCCCGGCTGTCATTGTCAGGCAGCGCAAGCCCTGGCGCCGAAAGGATGGTGTCTTCATGTACTTCGAAGATAATGCTGGTGTTATTGTCAATCCAAAGGGAGAAATGAAAGGGTCTGCAATTACTGGTCCTATTGGGAAGGAGTGTGCTGATCTATGGCCAAGGATTGCCAGTGCTGCTAATGCCATTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

140

Amino Acids

15.0

Weight (kDa)

10.48

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 21 - 140 1.2e-41 Ribosomal protein L14p/L23e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 264
AccI GTMKAC 1 cut(s) 58
AccII CGCG 1 cut(s) 18
AciI CCGC 2 cut(s) 18, 68
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 2 cut(s) 186, 386
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 2 cut(s) 113, 290
AgeI ACCGGT 1 cut(s) 61
AjnI CCWGG 1 cut(s) 260
AlwI GGATC 1 cut(s) 31
AoxI GGCC 2 cut(s) 186, 386
ApeKI GCWGC 2 cut(s) 250, 404
AsiGI ACCGGT 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 2 cut(s) 255, 267
AspS9I GGNCC 2 cut(s) 226, 356
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 88, 185
AsuII TTCGAA 1 cut(s) 294
AvaII GGWCC 2 cut(s) 226, 356
BalI TGGCCA 2 cut(s) 188, 388
BanI GGYRCC 1 cut(s) 264
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
BbsI GAAGAC 1 cut(s) 274
BbvI GCAGC 2 cut(s) 262, 391
BccI CCATC 1 cut(s) 269
BceAI ACGGC 1 cut(s) 142
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 1 cut(s) 233
BfoI RGCGCY 1 cut(s) 268
BisI GCNGC 3 cut(s) 69, 251, 405
BlsI GCNGC 3 cut(s) 70, 252, 406
Bme1390I CCNGG 2 cut(s) 233, 262
Bme18I GGWCC 2 cut(s) 226, 356
BmgT120I GGNCC 2 cut(s) 226, 356
BmiI GGNNCC 1 cut(s) 266
BmrFI CCNGG 2 cut(s) 233, 262
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 274
Bpu14I TTCGAA 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 233
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 260, 389
BsaWI WCCGGW 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 3 cut(s) 57, 358, 399
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 2 cut(s) 260, 389
BseGI GGATG 2 cut(s) 51, 280
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 3 cut(s) 57, 358, 399
BseXI GCAGC 2 cut(s) 262, 391
BseYI CCCAGC 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 18
BshFI GGCC 2 cut(s) 188, 388
BshNI GGYRCC 1 cut(s) 264
BshTI ACCGGT 1 cut(s) 61
BsiSI CCGG 2 cut(s) 62, 233
BsnI GGCC 2 cut(s) 188, 388
Bsp119I TTCGAA 1 cut(s) 294
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 23, 379
BspACI CCGC 2 cut(s) 18, 68
BspANI GGCC 2 cut(s) 188, 388
BspCNI CTCAG 1 cut(s) 134
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 266
BspPI GGATC 1 cut(s) 31
BspT104I TTCGAA 1 cut(s) 294
BspT107I GGYRCC 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 61
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 57, 358, 399
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 260, 389
BssMI GATC 2 cut(s) 23, 379
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 3 cut(s) 76, 83, 193
BstACI GRCGYC 1 cut(s) 265
BstAUI TGTACA 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 166, 257
BstDEI CTNAG 1 cut(s) 121
BstF5I GGATG 2 cut(s) 51, 280
BstFNI CGCG 1 cut(s) 18
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 255, 267
BstKTI GATC 2 cut(s) 26, 382
BstMBI GATC 2 cut(s) 23, 379
BstMWI GCNNNNNNNGC 3 cut(s) 256, 404, 410
BstNI CCWGG 1 cut(s) 262
BstSCI CCNGG 2 cut(s) 231, 260
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 2 cut(s) 262, 391
BstV2I GAAGAC 1 cut(s) 274
BsuRI GGCC 2 cut(s) 188, 388
BtsCI GGATG 2 cut(s) 51, 280
BtsIMutI CAGTG 4 cut(s) 50, 129, 189, 406
Cac8I GCNNGC 2 cut(s) 166, 257
CfoI GCGC 2 cut(s) 255, 267
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 2 cut(s) 226, 356
CseI GACGC 2 cut(s) 24, 157
Csp6I GTAC 2 cut(s) 112, 289
CspAI ACCGGT 1 cut(s) 61
CviAII CATG 3 cut(s) 178, 184, 286
CviJI RGCY 6 cut(s) 29, 188, 208, 236, 259, 388
CviKI_1 RGCY 6 cut(s) 29, 188, 208, 236, 259, 388
CviQI GTAC 2 cut(s) 112, 289
DdeI CTNAG 1 cut(s) 121
DinI GGCGCC 1 cut(s) 266
DpnI GATC 2 cut(s) 25, 381
DpnII GATC 2 cut(s) 23, 379
EaeI YGGCCR 2 cut(s) 186, 386
Eco130I CCWWGG 1 cut(s) 389
Eco47I GGWCC 2 cut(s) 226, 356
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 389
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 3 cut(s) 181, 187, 289
FaiI YATR 4 cut(s) 179, 185, 287, 385
FatI CATG 3 cut(s) 177, 183, 285
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 69, 251, 405
FokI GGATG 2 cut(s) 58, 287
Fsp4HI GCNGC 3 cut(s) 69, 251, 405
GlaI GCGC 2 cut(s) 254, 266
GluI GCNGC 3 cut(s) 69, 251, 405
GsaI CCCAGC 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 2 cut(s) 188, 388
HapII CCGG 2 cut(s) 62, 233
HgaI GACGC 2 cut(s) 24, 157
HhaI GCGC 2 cut(s) 255, 267
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 3 cut(s) 181, 187, 289
Hin6I GCGC 2 cut(s) 253, 265
HinP1I GCGC 2 cut(s) 253, 265
HinfI GANTC 2 cut(s) 132, 148
HpaII CCGG 2 cut(s) 62, 233
HphI GGTGA 2 cut(s) 88, 185
Hpy166II GTNNAC 2 cut(s) 59, 79
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 2 cut(s) 43, 196
Hpy8I GTNNAC 2 cut(s) 59, 79
Hpy99I CGWCG 2 cut(s) 76, 173
HpyAV CCTTC 4 cut(s) 121, 193, 217, 361
HpyCH4III ACNGT 3 cut(s) 76, 83, 193
HpyCH4V TGCA 1 cut(s) 347
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 404, 410
HpyF3I CTNAG 1 cut(s) 121
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 3 cut(s) 181, 187, 289
HspAI GCGC 2 cut(s) 253, 265
KasI GGCGCC 1 cut(s) 264
Kzo9I GATC 2 cut(s) 23, 379
Lsp1109I GCAGC 2 cut(s) 262, 391
MaeIII GTNAC 2 cut(s) 48, 173
MalI GATC 2 cut(s) 25, 381
MboI GATC 2 cut(s) 23, 379
MboII GAAGA 4 cut(s) 115, 232, 274, 308
MlsI TGGCCA 2 cut(s) 188, 388
MluCI AATT 1 cut(s) 348
MluNI TGGCCA 2 cut(s) 188, 388
Mly113I GGCGCC 1 cut(s) 265
MnlI CCTC 4 cut(s) 5, 14, 210, 239
Mox20I TGGCCA 2 cut(s) 188, 388
MroXI GAANNNNTTC 1 cut(s) 38
MscI TGGCCA 2 cut(s) 188, 388
MseI TTAA 1 cut(s) 421
MslI CAYNNNNRTG 2 cut(s) 122, 182
Msp20I TGGCCA 2 cut(s) 188, 388
MspI CCGG 2 cut(s) 62, 233
MspR9I CCNGG 2 cut(s) 233, 262
MvaI CCWGG 1 cut(s) 262
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 3 cut(s) 256, 404, 410
NarI GGCGCC 1 cut(s) 265
NciI CCSGG 1 cut(s) 233
NdeII GATC 2 cut(s) 23, 379
NlaIII CATG 3 cut(s) 181, 187, 289
NlaIV GGNNCC 1 cut(s) 266
NmuCI GTSAC 2 cut(s) 48, 173
NspV TTCGAA 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 2 cut(s) 132, 148
PflFI GACNNNGTC 1 cut(s) 179
PinAI ACCGGT 1 cut(s) 61
PkrI GCNGC 3 cut(s) 70, 252, 406
PluTI GGCGCC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 29
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 1 cut(s) 266
PspPI GGNCC 2 cut(s) 226, 356
PspPPI RGGWCCY 1 cut(s) 226
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 2 cut(s) 113, 290
RsaNI GTAC 2 cut(s) 112, 289
RseI CAYNNNNRTG 2 cut(s) 122, 182
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 3 cut(s) 69, 251, 405
Sau3AI GATC 2 cut(s) 23, 379
Sau96I GGNCC 2 cut(s) 226, 356
ScrFI CCNGG 2 cut(s) 233, 262
SetI ASST 2 cut(s) 111, 228
SfoI GGCGCC 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 294
SinI GGWCC 2 cut(s) 226, 356
SmiMI CAYNNNNRTG 2 cut(s) 122, 182
Sse9I AATT 1 cut(s) 348
SsiI CCGC 2 cut(s) 18, 68
SspDI GGCGCC 1 cut(s) 264
StyD4I CCNGG 2 cut(s) 231, 260
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 3 cut(s) 76, 83, 193
TaqI TCGA 2 cut(s) 5, 294
TasI AATT 1 cut(s) 348
TatI WGTACW 2 cut(s) 111, 288
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 132, 148
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TscAI CASTG 4 cut(s) 57, 129, 196, 406
TseFI GTSAC 2 cut(s) 48, 173
TseI GCWGC 2 cut(s) 250, 404
Tsp45I GTSAC 2 cut(s) 48, 173
TspDTI ATGAA 2 cut(s) 274, 350
TspRI CASTG 4 cut(s) 57, 129, 196, 406
Tth111I GACNNNGTC 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 226, 356
XmiI GTMKAC 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.