Rh3BG330400

Nonspecific lipid-transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0007035
Physical Location & Seq
Reverse (-)
34545519 .. 34546384
866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG330400.1

Sequence Viewer

Length: 345 bp
ATGAAGTTGTCAACTAGCAGCGCCGTGTCGGTTTTGGTACTTGTATTCATCATCTTCATGAGTATTGCCACTACAGACTTGGTGATGGTCGCAGCAGGCCGAGTACCGGAGCTTGCACACCAGGCAGCTGAGGCAGATACGACATGCGATCCGGTGCAGTTGAGTCCATGTTTAGATCCAATCCAGCATGGTTCGAAGCCGTCGAGCACCTGCTGCCAGAAGCTCAAGGAGCAGATTCCATGCCTTTGTGGATACATCAAGAACCCAGCCTTCAAGCAATATGTCACCGGTCCCAACGCCAAAAAACTCTCTGCCAACTGTGGTGTTCAGTACCCTCGCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

114

Amino Acids

12.19

Weight (kDa)

8.44

Isoelectric Point (pI)

22.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 38 - 111 1.3e-10 Probable lipid transfer
Tryp_alpha_amyl PF00234 52 - 114 1.2e-10 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 218
Acc36I ACCTGC 1 cut(s) 218
AclWI GGATC 2 cut(s) 143, 170
AfaI GTAC 3 cut(s) 39, 105, 332
AfiI CCNNNNNNNGG 1 cut(s) 106
AgeI ACCGGT 1 cut(s) 287
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 3 cut(s) 112, 128, 223
AluI AGCT 3 cut(s) 112, 128, 223
Alw21I GWGCWC 1 cut(s) 209
AlwI GGATC 2 cut(s) 143, 170
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 4 cut(s) 18, 92, 125, 213
AsiGI ACCGGT 1 cut(s) 287
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 2 cut(s) 94, 277
AsuII TTCGAA 1 cut(s) 194
AvaII GGWCC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 209
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 4 cut(s) 30, 104, 137, 200
BccI CCATC 1 cut(s) 79
BceAI ACGGC 2 cut(s) 8, 184
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 1 cut(s) 245
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 72
BfoI RGCGCY 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 218
BfuI GTATCC 1 cut(s) 245
BisI GCNGC 4 cut(s) 19, 93, 126, 214
BlsI GCNGC 4 cut(s) 20, 94, 127, 215
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 129
Bpu14I TTCGAA 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 209
BsaWI WCCGGW 3 cut(s) 106, 151, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse118I RCCGGY 1 cut(s) 287
BseBI CCWGG 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMII CTCAG 1 cut(s) 120
BseXI GCAGC 4 cut(s) 30, 104, 137, 200
BseYI CCCAGC 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 99
BshTI ACCGGT 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 107, 152, 288
BslFI GGGAC 1 cut(s) 276
BslI CCNNNNNNNGG 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 276
BsnI GGCC 1 cut(s) 99
Bsp119I TTCGAA 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 2 cut(s) 148, 175
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 1 cut(s) 121
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 292
BspMI ACCTGC 1 cut(s) 218
BspPI GGATC 2 cut(s) 143, 170
BspT104I TTCGAA 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 287
BssMI GATC 2 cut(s) 148, 175
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 320
BstAPI GCANNNNNTGC 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 97, 114
BstDEI CTNAG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 2 cut(s) 151, 178
BstMBI GATC 2 cut(s) 148, 175
BstMWI GCNNNNNNNGC 4 cut(s) 122, 131, 213, 229
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 120
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 30, 104, 137, 200
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 1 cut(s) 99
BveI ACCTGC 1 cut(s) 218
Cac8I GCNNGC 2 cut(s) 97, 114
CciI TCATGA 1 cut(s) 57
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 290
Csp6I GTAC 3 cut(s) 38, 104, 331
CspAI ACCGGT 1 cut(s) 287
CviAII CATG 5 cut(s) 58, 144, 168, 188, 240
CviJI RGCY 6 cut(s) 99, 112, 128, 199, 223, 269
CviKI_1 RGCY 6 cut(s) 99, 112, 128, 199, 223, 269
CviQI GTAC 3 cut(s) 38, 104, 331
DdeI CTNAG 1 cut(s) 129
DpnI GATC 2 cut(s) 150, 177
DpnII GATC 2 cut(s) 148, 175
Eco47I GGWCC 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 120
FaeI CATG 5 cut(s) 61, 147, 171, 191, 243
FaiI YATR 6 cut(s) 59, 145, 169, 189, 241, 282
FaqI GGGAC 1 cut(s) 276
FatI CATG 5 cut(s) 57, 143, 167, 187, 239
Fnu4HI GCNGC 4 cut(s) 19, 93, 126, 214
Fsp4HI GCNGC 4 cut(s) 19, 93, 126, 214
FspBI CTAG 1 cut(s) 15
GlaI GCGC 1 cut(s) 22
GluI GCNGC 4 cut(s) 19, 93, 126, 214
GsaI CCCAGC 1 cut(s) 269
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 1 cut(s) 99
HapII CCGG 3 cut(s) 107, 152, 288
HhaI GCGC 1 cut(s) 23
Hin1II CATG 5 cut(s) 61, 147, 171, 191, 243
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 2 cut(s) 163, 235
HpaII CCGG 3 cut(s) 107, 152, 288
HphI GGTGA 2 cut(s) 94, 277
Hpy166II GTNNAC 1 cut(s) 12
Hpy188III TCNNGA 2 cut(s) 58, 259
Hpy8I GTNNAC 1 cut(s) 12
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4V TGCA 2 cut(s) 116, 157
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 131, 213, 229
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 5 cut(s) 61, 147, 171, 191, 243
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 2 cut(s) 148, 175
LmnI GCTCC 2 cut(s) 109, 229
Lsp1109I GCAGC 4 cut(s) 30, 104, 137, 200
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 283
MalI GATC 2 cut(s) 150, 177
MboI GATC 2 cut(s) 148, 175
MboII GAAGA 1 cut(s) 46
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 209
MlyI GAGTC 1 cut(s) 172
MnlI CCTC 2 cut(s) 124, 345
MseI TTAA 1 cut(s) 343
MslI CAYNNNNRTG 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 3 cut(s) 107, 152, 288
MspR9I CCNGG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 4 cut(s) 122, 131, 213, 229
NdeII GATC 2 cut(s) 148, 175
NlaIII CATG 5 cut(s) 61, 147, 171, 191, 243
NlaIV GGNNCC 1 cut(s) 292
NmeAIII GCCGAG 1 cut(s) 125
NmuCI GTSAC 1 cut(s) 283
NspI RCATGY 1 cut(s) 147
NspV TTCGAA 1 cut(s) 194
PagI TCATGA 1 cut(s) 57
PaqCI CACCTGC 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PfeI GAWTC 1 cut(s) 235
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 4 cut(s) 20, 94, 127, 215
PleI GAGTC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 120
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 1 cut(s) 290
PsuI RGATCY 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 3 cut(s) 39, 105, 332
RsaNI GTAC 3 cut(s) 38, 104, 331
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 1 cut(s) 343
SatI GCNGC 4 cut(s) 19, 93, 126, 214
Sau3AI GATC 2 cut(s) 148, 175
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 1 cut(s) 209
SetI ASST 4 cut(s) 114, 130, 212, 225
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 194
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 320
TaqI TCGA 2 cut(s) 194, 203
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 1 cut(s) 343
Tru9I TTAA 1 cut(s) 343
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 4 cut(s) 18, 92, 125, 213
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 3 cut(s) 17, 37, 46
VpaK11BI GGWCC 1 cut(s) 290
XceI RCATGY 1 cut(s) 147
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.