Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0008359

Physical Location & Seq

Genomic Coordinates Reverse (-)

31302610 .. 31305812

Gene Structure 3203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0178400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGTTGTCATTGAAGGTTTGTGCAAAGCTGGAAAAATTGAACCTGCAAGAGATGTCTTCTCTTGCTTATCAGCATAAAGGAGTTCAGCCTAATGTGAGAACATACACTATAATGATTCAGGGCCTTTGTCATCATGGCTTAATAATTGAAGCAGAACAGTTGCTGAGAGAAATGGGAGGGAAAGGATGTTCTCCAAATGGTTGCACCTATAACACCATTATCCAAGGTTTTATCAATAACAAGGAGACATCAAGGGCTACGAGACTTATTCAAGAAATGCTTGAGAGGGGTTTCTCTGCAGATGCATCAACTATGGAATTGATTGTTGATTTATTGTCGAAGGATACAATAGATCCTGACAGCTTTTGCTTAATTGCTTTGTTGTTTTTGAGAATTGATTGCATTGGACTACTGGGAGTTGGAATGGGTGTTGGGGAGGCTAACCCCCATTTGGACTCATCATCTAGGACAGTAGCATCATTTACTAGCAGTCTTTCTGAATCAAGATTGGGCAAGAAGCCCTTGGAAATTGCATGCATTATCTTGTTTCTTCAGTTGGTTTCATCACCAAAACTTGCTTCTGCTGATTCTACACAGGCAGAGGCACTTCTCAAATGGCAAGCCAGCTTTCTGAATCAAACCGGGAATAACAATCTCACCTCGTGGACTTCCCTCCCCGGAAACGCCACCAATTCATCAACTGCATGCAATGTTTGGACCGGTATTTCATGCAATGCTGCCGGAAGTGTCAACCGGATAAACCTTACCAATTCAGGTATAAAAGGTACGCTACATGAACTCTCATTCCAGTCCTTCCCAGATCTTGAATATGTTGACCTCAGTGTCAATGAACTTTTCGACACCATCCCACCTCAGATTAGTTCCCTCTCGAAACTCATCTACCTTGATGTATCTTATAATCAGTTGACTGGGATCATCCCACCAGAAATTGGTCTCCTAACAAATCTTCAAGTCCTCCACCTAAACGGAAACCAGTTAAATGGCTCGATTCCTCAAGAAATGAGCCAGCTCAAATCTCTTTATGAGCTTGCTCTGAACACCAACAATCCAGAAGGGTCTATTCCAGCCTTCCTGGGTAATTTGACCAATATGACAAATTTGTATCTCTTTGGAAATCAGCTTTCTGGTGTCATTCCTACTGAAATCGGAAACCTATCAAAATTGGTTGAATTGCACTTGGATGACAACCATTTAACAGGTCCTATCCCTCCAAGTTTTGGAAACTTGGAAAATCTAACTGTGTTGTACTTAGCCTACAACATTTCTGGTTCTATCCCTTCAGAAATAGGAAAGATGAAGTCTCTTGTGCAACTGAGCATTCAATCAAACAATCTTTCTGGTTCAATTCCGGCATCCATAGGTGATCTGCCAAATCTTACCCTTCTTTATCTCTATTCAAACAAAATTTCAGGCAGTATTCCTAAGGAGATAGGGAATTTGAGATCTATGGTGGATCTACAGTTGAGCCAGAATCAACTAAATGGCTCGGTTCCAACTTCCTTGGGTGACTTGGAAAACTTAGTAAGCTTGTTCCTCCGTGATAACCAACTTTCTGGCTCCATCCCCCAAGAGATAGGAAATCTCACAAAGATGACAATTGCGCAATTTGAAACCAACCGATTTTCTGCTTATTTGCCCCAAAACATTTGCAGAGGTGGATTGCTTCAAAACTTCACAGCATACAACAATGATTTGATAGGTCCAATCCCCGAGAGCTTGAAAAATTGCCAGAGCTTATTCAGAGTATGTCTTGAAGGGAACCAATTGACTGGCAATGTATCTCAAGACTTTGGAGTGTATCCAAATCTTGATTATATAAATCTAAGCAACAATAAGTTGTATGGTGAAATCTCACAAAACTGGGGACAATTCCCACAGTTAACGAGCCTCCTAATTGCGGGTAACAACCTTACCGGTGGCATACCACCAGAGCTTGGCAATGCAACTCAAATTCAGTTGCTTGATCTCTCTTCCAATCATTTAGTTGGTGTGATTCCAGAAGATCTTGGGAGGCTAAGTTTGGTGAATCTGAAGTTGGGTGATAATCAACTTTCTGGTCCTATACCCTCCACATTTGCATCTCTGACTGACCTTGAATATATAGACCTGTCCACCAACAAAATTAACGAGTCAATTCTAAGCTTTATAGGTGACATGTCAAAACTAATCTACTTGAATCTGAGCAACAACAAGTTCAGTCAGGAGATTCCATTACAGTTGGGGAACTTTTTTCAACTGTCCCAGCTAGATTTAAAGTCGCAACTCACTGGAGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000447 GO:0000460 GO:0000461 GO:0000462 GO:0000466 GO:0000469 GO:0000478 GO:0000479 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0015935 GO:0016043 GO:0016070 GO:0016072 GO:0022613 GO:0022626 GO:0022627 GO:0030490 GO:0031123 GO:0031125 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0042788 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043628 GO:0044085 GO:0044237 GO:0044238 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribosomal_S21e

Protein Analysis

765

Amino Acids

83.07

Weight (kDa)

4.87

Isoelectric Point (pI)

30.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	24 - 77	7e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	26 - 58	3.6e-11	PPR repeat
PPR_2	PF13041	30 - 78	3.3e-14	PPR repeat family
PPR	PF01535	34 - 63	6.4e-08	PPR repeat
PPR_2	PF13041	65 - 112	7.1e-08	PPR repeat family
LRR_14	PF23598	270 - 376	1.1e-09	Leucine-rich repeat region
LRR_8	PF13855	274 - 333	7.4e-08	Leucine rich repeat
LRR_4	PF12799	298 - 334	5.8e-06	Leucine Rich repeats (2 copies)
LRR_14	PF23598	385 - 470	7.8e-09	Leucine-rich repeat region
LRR_8	PF13855	394 - 452	2.6e-07	Leucine rich repeat
LRR_8	PF13855	442 - 500	1.2e-06	Leucine rich repeat
LRR_14	PF23598	459 - 538	7.5e-08	Leucine-rich repeat region
LRR_8	PF13855	464 - 524	8.6e-06	Leucine rich repeat
LRR_8	PF13855	680 - 739	4.8e-06	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53890 AT3G53890 AT5G27700
fragaria_vesca	FvH4_4g17990 FvH4_4g17990 FvH4_4g17990 FvH4_4g17990 FvH4_6g28030 FvH4_6g28030
malus_domestica	MD11G1125000.v1.1 MD13G1168900.v1.1 MD16G1170000.v1.1
prunus_persica	Prupe.1G137200_v2.0.a1 Prupe.6G093700_v2.0.a1
pyrus_communis	pycom13g14450 pycom16g14260
rosa_chinensis	RchiOBHm_Chr4g0422191
rosa_multiflora	Rmu_sc0000801.1_g000004 Rmu_sc0003930.1_g000011
rosa_roxburghii	Rroxscaffold_5G00364310
rosa_rugosa	Rorug04G0178400
rosa_samantha	Rh4CG252500
rosa_wichuraiana	Rw4G020550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	918
AasI	GACNNNNNNGTC	1 cut(s)	842
Acc16I	TGCGCA	1 cut(s)	1623
Acc36I	ACCTGC	1 cut(s)	51
AccB7I	CCANNNNNTGG	3 cut(s)	950, 1238, 1955
AciI	CCGC	1 cut(s)	1919
AclWI	GGATC	3 cut(s)	347, 941, 1482
AcsI	RAATTY	4 cut(s)	1117, 1425, 1456, 1971
AcuI	CTGAAG	3 cut(s)	536, 1284, 2072
AdeI	CACNNNGTG	1 cut(s)	663
AfaI	GTAC	2 cut(s)	787, 1268
AfiI	CCNNNNNNNGG	5 cut(s)	451, 950, 1238, 1918, 1955
AflIII	ACRYGT	1 cut(s)	2175
AgeI	ACCGGT	2 cut(s)	719, 1934
AjnI	CCWGG	1 cut(s)	1092
AjuI	GAANNNNNNNTTGG	4 cut(s)	562, 594, 2039, 2071
AloI	GAACNNNNNNTCC	4 cut(s)	172, 204, 789, 821
Alw26I	GTCTC	4 cut(s)	239, 256, 959, 1326
AlwI	GGATC	3 cut(s)	347, 941, 1482
AlwNI	CAGNNNCTG	1 cut(s)	163
Ama87I	CYCGRG	1 cut(s)	1730
AoxI	GGCC	1 cut(s)	121
ApeKI	GCWGC	1 cut(s)	737
ApoI	RAATTY	4 cut(s)	1117, 1425, 1456, 1971
AsiGI	ACCGGT	2 cut(s)	719, 1934
AspLEI	GCGC	1 cut(s)	1624
AspS9I	GGNCC	5 cut(s)	121, 717, 1220, 1721, 2078
AsuC2I	CCSGG	2 cut(s)	643, 678
AsuHPI	GGTGA	8 cut(s)	558, 649, 1394, 1538, 1877, 2056, 2072, 2183
AvaI	CYCGRG	1 cut(s)	1730
AvaII	GGWCC	4 cut(s)	717, 1220, 1721, 2078
AxyI	CCTNAGG	1 cut(s)	1443
BauI	CACGAG	1 cut(s)	661
BbsI	GAAGAC	1 cut(s)	48
BbvI	GCAGC	1 cut(s)	724
BccI	CCATC	2 cut(s)	872, 1589
BciT130I	CCWGG	1 cut(s)	1094
BciVI	GTATCC	2 cut(s)	337, 1830
BcnI	CCSGG	2 cut(s)	643, 678
BcoDI	GTCTC	4 cut(s)	239, 256, 959, 1326
BfaI	CTAG	3 cut(s)	465, 486, 2267
BfmI	CTRYAG	2 cut(s)	297, 1478
BfuAI	ACCTGC	1 cut(s)	51
BfuI	GTATCC	2 cut(s)	337, 1830
BglII	AGATCT	3 cut(s)	820, 1463, 2023
BisI	GCNGC	1 cut(s)	738
BlsI	GCNGC	1 cut(s)	739
Bme1390I	CCNGG	3 cut(s)	643, 678, 1094
Bme18I	GGWCC	4 cut(s)	717, 1220, 1721, 2078
BmeT110I	CYCGRG	1 cut(s)	1730
BmgT120I	GGNCC	5 cut(s)	121, 717, 1220, 1721, 2078
BmiI	GGNNCC	3 cut(s)	1512, 1579, 1781
BmrFI	CCNGG	3 cut(s)	643, 678, 1094
BmrI	ACTGGG	3 cut(s)	422, 939, 1891
BmsI	GCATC	5 cut(s)	292, 314, 485, 1382, 2108
BmuI	ACTGGG	3 cut(s)	422, 939, 1891
BpiI	GAAGAC	1 cut(s)	48
BpuEI	CTTGAG	3 cut(s)	302, 999, 1788
BpuMI	CCSGG	2 cut(s)	643, 678
BsaI	GGTCTC	1 cut(s)	959
BsaJI	CCNNGG	5 cut(s)	223, 522, 676, 1093, 1521
BsaWI	WCCGGW	3 cut(s)	719, 753, 1934
BsaXI	ACNNNNNCTCC	2 cut(s)	2023, 2053
Bsc4I	CCNNNNNNNGG	5 cut(s)	451, 950, 1238, 1918, 1955
Bse118I	RCCGGY	2 cut(s)	719, 1934
Bse1I	ACTGG	7 cut(s)	417, 808, 934, 994, 1795, 1886, 2293
Bse21I	CCTNAGG	1 cut(s)	1443
Bse3DI	GCAATG	4 cut(s)	715, 739, 1801, 1966
BseBI	CCWGG	1 cut(s)	1094
BseDI	CCNNGG	5 cut(s)	223, 522, 676, 1093, 1521
BseGI	GGATG	6 cut(s)	191, 864, 936, 1207, 1373, 1581
BseLI	CCNNNNNNNGG	5 cut(s)	451, 950, 1238, 1918, 1955
BseMI	GCAATG	4 cut(s)	715, 739, 1801, 1966
BseMII	CTCAG	5 cut(s)	155, 853, 887, 1325, 2192
BseNI	ACTGG	7 cut(s)	417, 808, 934, 994, 1795, 1886, 2293
BseXI	GCAGC	1 cut(s)	724
BseYI	CCCAGC	1 cut(s)	2262
BshFI	GGCC	1 cut(s)	123
BshTI	ACCGGT	2 cut(s)	719, 1934
BsiHKCI	CYCGRG	1 cut(s)	1730
BsiSI	CCGG	7 cut(s)	642, 678, 720, 741, 754, 1370, 1935
BslFI	GGGAC	2 cut(s)	1899, 2245
BslI	CCNNNNNNNGG	5 cut(s)	451, 950, 1238, 1918, 1955
BsmAI	GTCTC	4 cut(s)	239, 256, 959, 1326
BsmFI	GGGAC	2 cut(s)	1899, 2245
BsmI	GAATGC	1 cut(s)	1338
BsnI	GGCC	1 cut(s)	123
Bso31I	GGTCTC	1 cut(s)	959
BsoBI	CYCGRG	1 cut(s)	1730
Bsp143I	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
BspACI	CCGC	1 cut(s)	1919
BspANI	GGCC	1 cut(s)	123
BspCNI	CTCAG	5 cut(s)	156, 852, 886, 1326, 2193
BspLI	GGNNCC	3 cut(s)	1512, 1579, 1781
BspMAI	CTGCAG	1 cut(s)	301
BspMI	ACCTGC	1 cut(s)	51
BspPI	GGATC	3 cut(s)	347, 941, 1482
BspTNI	GGTCTC	1 cut(s)	959
BsrDI	GCAATG	4 cut(s)	715, 739, 1801, 1966
BsrFI	RCCGGY	2 cut(s)	719, 1934
BsrI	ACTGG	7 cut(s)	417, 808, 934, 994, 1795, 1886, 2293
BssAI	RCCGGY	2 cut(s)	719, 1934
BssECI	CCNNGG	5 cut(s)	223, 522, 676, 1093, 1521
BssMI	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
BssSI	CACGAG	1 cut(s)	661
BssT1I	CCWWGG	3 cut(s)	223, 522, 1521
Bst2BI	CACGAG	1 cut(s)	661
Bst2UI	CCWGG	1 cut(s)	1094
Bst4CI	ACNGT	7 cut(s)	159, 472, 1261, 1482, 1899, 2238, 2259
Bst6I	CTCTTC	1 cut(s)	1996
BstC8I	GCNNGC	6 cut(s)	535, 621, 625, 706, 1028, 1050
BstF5I	GGATG	6 cut(s)	191, 864, 936, 1207, 1373, 1581
BstHHI	GCGC	1 cut(s)	1624
BstKTI	GATC	8 cut(s)	355, 823, 936, 1387, 1466, 1477, 1987, 2026
BstMAI	GTCTC	4 cut(s)	239, 256, 959, 1326
BstMBI	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
BstNI	CCWGG	1 cut(s)	1094
BstNSI	RCATGY	3 cut(s)	537, 708, 2179
BstSCI	CCNGG	3 cut(s)	641, 676, 1092
BstSFI	CTRYAG	2 cut(s)	297, 1478
BstV1I	GCAGC	1 cut(s)	724
BstV2I	GAAGAC	1 cut(s)	48
BstX2I	RGATCY	5 cut(s)	352, 820, 1463, 1474, 2023
BstXI	CCANNNNNNTGG	3 cut(s)	1001, 1574, 1790
BstYI	RGATCY	5 cut(s)	352, 820, 1463, 1474, 2023
Bsu36I	CCTNAGG	1 cut(s)	1443
BsuI	GTATCC	2 cut(s)	337, 1830
BsuRI	GGCC	1 cut(s)	123
BtsCI	GGATG	6 cut(s)	191, 864, 936, 1207, 1373, 1581
BtsIMutI	CAGTG	2 cut(s)	847, 2286
BveI	ACCTGC	1 cut(s)	51
Cac8I	GCNNGC	6 cut(s)	535, 621, 625, 706, 1028, 1050
CaiI	CAGNNNCTG	1 cut(s)	163
CfoI	GCGC	1 cut(s)	1624
Cfr10I	RCCGGY	2 cut(s)	719, 1934
Cfr13I	GGNCC	5 cut(s)	121, 717, 1220, 1721, 2078
Csp6I	GTAC	2 cut(s)	786, 1267
CspAI	ACCGGT	2 cut(s)	719, 1934
CviAII	CATG	6 cut(s)	134, 534, 705, 729, 794, 2176
CviQI	GTAC	2 cut(s)	786, 1267
DpnI	GATC	8 cut(s)	354, 822, 935, 1386, 1465, 1476, 1986, 2025
DpnII	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
DraI	TTTAAA	1 cut(s)	2274
DraIII	CACNNNGTG	1 cut(s)	663
DrdI	GACNNNNNNGTC	1 cut(s)	842
DseDI	GACNNNNNNGTC	1 cut(s)	842
Eam1104I	CTCTTC	1 cut(s)	1996
EarI	CTCTTC	1 cut(s)	1996
Eco130I	CCWWGG	3 cut(s)	223, 522, 1521
Eco31I	GGTCTC	1 cut(s)	959
Eco47I	GGWCC	4 cut(s)	717, 1220, 1721, 2078
Eco57I	CTGAAG	3 cut(s)	536, 1284, 2072
Eco81I	CCTNAGG	1 cut(s)	1443
Eco88I	CYCGRG	1 cut(s)	1730
EcoO109I	RGGNCCY	2 cut(s)	121, 1220
EcoRII	CCWGG	1 cut(s)	1092
EcoT14I	CCWWGG	3 cut(s)	223, 522, 1521
EcoT22I	ATGCAT	2 cut(s)	307, 539
ErhI	CCWWGG	3 cut(s)	223, 522, 1521
FaeI	CATG	6 cut(s)	137, 537, 708, 732, 797, 2179
FalI	AAGNNNNNCTT	4 cut(s)	264, 296, 506, 538
FaqI	GGGAC	2 cut(s)	1899, 2245
FatI	CATG	6 cut(s)	133, 533, 704, 728, 793, 2175
FauI	CCCGC	1 cut(s)	1912
Fnu4HI	GCNGC	1 cut(s)	738
FokI	GGATG	6 cut(s)	198, 851, 923, 1214, 1360, 1568
Fsp4HI	GCNGC	1 cut(s)	738
FspBI	CTAG	3 cut(s)	465, 486, 2267
FspI	TGCGCA	1 cut(s)	1623
GlaI	GCGC	1 cut(s)	1623
GluI	GCNGC	1 cut(s)	738
GsaI	CCCAGC	1 cut(s)	2266
HaeIII	GGCC	1 cut(s)	123
HapII	CCGG	7 cut(s)	642, 678, 720, 741, 754, 1370, 1935
HhaI	GCGC	1 cut(s)	1624
Hin1II	CATG	6 cut(s)	137, 537, 708, 732, 797, 2179
Hin6I	GCGC	1 cut(s)	1622
HinP1I	GCGC	1 cut(s)	1622
HincII	GTYRAC	4 cut(s)	751, 835, 927, 1902
HindII	GTYRAC	4 cut(s)	751, 835, 927, 1902
HindIII	AAGCTT	2 cut(s)	1546, 2161
HpaI	GTTAAC	1 cut(s)	1902
HpaII	CCGG	7 cut(s)	642, 678, 720, 741, 754, 1370, 1935
HphI	GGTGA	8 cut(s)	558, 649, 1394, 1538, 1877, 2056, 2072, 2183
Hpy166II	GTNNAC	6 cut(s)	666, 751, 835, 927, 1902, 2133
Hpy8I	GTNNAC	6 cut(s)	666, 751, 835, 927, 1902, 2133
HpyAV	CCTTC	8 cut(s)	7, 334, 823, 1067, 1099, 1308, 1412, 1769
HpyCH4III	ACNGT	7 cut(s)	159, 472, 1261, 1482, 1899, 2238, 2259
Hsp92II	CATG	6 cut(s)	137, 537, 708, 732, 797, 2179
HspAI	GCGC	1 cut(s)	1622
KspAI	GTTAAC	1 cut(s)	1902
Kzo9I	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
LmnI	GCTCC	1 cut(s)	1583
Lsp1109I	GCAGC	1 cut(s)	724
LweI	GCATC	5 cut(s)	292, 314, 485, 1382, 2108
MaeI	CTAG	3 cut(s)	465, 486, 2267
MaeIII	GTNAC	3 cut(s)	1526, 1922, 2171
MalI	GATC	8 cut(s)	354, 822, 935, 1386, 1465, 1476, 1986, 2025
MboI	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
MboII	GAAGA	5 cut(s)	48, 542, 959, 1983, 2033
MfeI	CAATTG	2 cut(s)	1617, 1784
MflI	RGATCY	5 cut(s)	352, 820, 1463, 1474, 2023
MlyI	GAGTC	2 cut(s)	449, 2159
MmeI	TCCRAC	2 cut(s)	400, 1538
Mph1103I	ATGCAT	2 cut(s)	307, 539
MseI	TTAA	7 cut(s)	140, 371, 998, 1214, 1901, 2145, 2273
MslI	CAYNNNNRTG	3 cut(s)	110, 1200, 1610
MspI	CCGG	7 cut(s)	642, 678, 720, 741, 754, 1370, 1935
MspR9I	CCNGG	3 cut(s)	643, 678, 1094
MunI	CAATTG	2 cut(s)	1617, 1784
Mva1269I	GAATGC	1 cut(s)	1338
MvaI	CCWGG	1 cut(s)	1094
NciI	CCSGG	2 cut(s)	643, 678
NdeII	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
NlaIII	CATG	6 cut(s)	137, 537, 708, 732, 797, 2179
NlaIV	GGNNCC	3 cut(s)	1512, 1579, 1781
NmuCI	GTSAC	2 cut(s)	1526, 2171
NsbI	TGCGCA	1 cut(s)	1623
NsiI	ATGCAT	2 cut(s)	307, 539
NspI	RCATGY	3 cut(s)	537, 708, 2179
PaeI	GCATGC	2 cut(s)	537, 708
PciI	ACATGT	1 cut(s)	2175
PctI	GAATGC	1 cut(s)	1338
PflMI	CCANNNNNTGG	3 cut(s)	950, 1238, 1955
PinAI	ACCGGT	2 cut(s)	719, 1934
PkrI	GCNGC	1 cut(s)	739
PleI	GAGTC	2 cut(s)	449, 2158
PpsI	GAGTC	2 cut(s)	449, 2158
PpuMI	RGGWCCY	1 cut(s)	1220
PscI	ACATGT	1 cut(s)	2175
PsiI	TTATAA	1 cut(s)	918
Psp5II	RGGWCCY	1 cut(s)	1220
Psp6I	CCWGG	1 cut(s)	1092
PspFI	CCCAGC	1 cut(s)	2262
PspGI	CCWGG	1 cut(s)	1092
PspN4I	GGNNCC	3 cut(s)	1512, 1579, 1781
PspPI	GGNCC	5 cut(s)	121, 717, 1220, 1721, 2078
PspPPI	RGGWCCY	1 cut(s)	1220
PstI	CTGCAG	1 cut(s)	301
PstNI	CAGNNNCTG	1 cut(s)	163
PsuI	RGATCY	5 cut(s)	352, 820, 1463, 1474, 2023
RsaI	GTAC	2 cut(s)	787, 1268
RsaNI	GTAC	2 cut(s)	786, 1267
RseI	CAYNNNNRTG	3 cut(s)	110, 1200, 1610
SaqAI	TTAA	7 cut(s)	140, 371, 998, 1214, 1901, 2145, 2273
SatI	GCNGC	1 cut(s)	738
Sau3AI	GATC	8 cut(s)	352, 820, 933, 1384, 1463, 1474, 1984, 2023
Sau96I	GGNCC	5 cut(s)	121, 717, 1220, 1721, 2078
SchI	GAGTC	2 cut(s)	449, 2159
ScrFI	CCNGG	3 cut(s)	643, 678, 1094
SfaNI	GCATC	5 cut(s)	292, 314, 485, 1382, 2108
SfcI	CTRYAG	2 cut(s)	297, 1478
SinI	GGWCC	4 cut(s)	717, 1220, 1721, 2078
SmiMI	CAYNNNNRTG	3 cut(s)	110, 1200, 1610
SmlI	CTYRAG	3 cut(s)	281, 1014, 1803
SmoI	CTYRAG	3 cut(s)	281, 1014, 1803
SphI	GCATGC	2 cut(s)	537, 708
SsiI	CCGC	1 cut(s)	1919
SspMI	CTAG	3 cut(s)	465, 486, 2267
StyD4I	CCNGG	3 cut(s)	641, 676, 1092
StyI	CCWWGG	3 cut(s)	223, 522, 1521
TaaI	ACNGT	7 cut(s)	159, 472, 1261, 1482, 1899, 2238, 2259
TaqI	TCGA	4 cut(s)	338, 858, 890, 1007
TatI	WGTACW	1 cut(s)	1266
Tru1I	TTAA	7 cut(s)	140, 371, 998, 1214, 1901, 2145, 2273
Tru9I	TTAA	7 cut(s)	140, 371, 998, 1214, 1901, 2145, 2273
TscAI	CASTG	2 cut(s)	847, 2293
TseFI	GTSAC	2 cut(s)	1526, 2171
TseI	GCWGC	1 cut(s)	737
Tsp45I	GTSAC	2 cut(s)	1526, 2171
TspDTI	ATGAA	6 cut(s)	552, 684, 717, 810, 864, 1331
TspGWI	ACGGA	2 cut(s)	1002, 1547
TspRI	CASTG	2 cut(s)	847, 2293
Van91I	CCANNNNNTGG	3 cut(s)	950, 1238, 1955
VpaK11BI	GGWCC	4 cut(s)	717, 1220, 1721, 2078
XapI	RAATTY	4 cut(s)	1117, 1425, 1456, 1971
XceI	RCATGY	3 cut(s)	537, 708, 2179
XspI	CTAG	3 cut(s)	465, 486, 2267
Zsp2I	ATGCAT	2 cut(s)	307, 539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.