Rorug04G0205000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007436
Physical Location & Seq
Forward (+)
35743517 .. 35744425
909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0205000.1

Sequence Viewer

Length: 645 bp
ATGCTAACAGATGGAAGAATCGTTGCAGTAAATAGGTCTACAAAAGTGGACGAAGGTAGACTTTCACAGTTTATAAATGAGGTTGTTATTCTTTCACAAATCAACCACATGAATGTGGTTAAACTATTGGGTTGTGGTTTAGAGATAGAGGTTCCTCTTTTGGTCTATGAGTTTATTCCGAATGGATCCCTTTCCCAATATATCCATGAGCAGCACGAGGATTTTCCACTTACGTGGGAATTGCGCTTAAGGATTGCCACGGAAATTGCAGGAGCTCTTTCCTACTTACACGATGCAACTTCAATTCCCATATTTCATAGAGACATCAAGTTGAAAAATATATTGCTAGATGAAAAATACAGAGCAAAAATTGCCGACTTTGGAACTTCTAGATCAGTTGCCATAGACCAAACTCACTTGACCACACTTGTATATGGGACATTTGGTTACTTGGACCCAGAATACTTCCAGTCGAGCCAATTTACGGATAAAAGTGATGTGTATAGCTTTGGAGTGGTCCTTGTTAAACTCTTGAGTGGACAAAAACCAATCTCTGTGACAAGATCACAAGACGAAGGTCGGAGTCTTGCAACACATTTCATTATTTTGATGCAGGATGATCGGCTATTTGATCGAAGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

24.31

Weight (kDa)

5.62

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 196 2.5e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 8 - 190 1.8e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 74
AccI GTMKAC 2 cut(s) 38, 58
AclWI GGATC 2 cut(s) 180, 193
AfiI CCNNNNNNNGG 1 cut(s) 484
AflII CTTAAG 1 cut(s) 247
AgsI TTSAA 2 cut(s) 303, 334
AleI CACNNNNGTG 1 cut(s) 232
AluBI AGCT 2 cut(s) 275, 507
AluI AGCT 2 cut(s) 275, 507
Alw21I GWGCWC 1 cut(s) 277
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 2 cut(s) 180, 193
ApeKI GCWGC 1 cut(s) 211
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 2 cut(s) 454, 517
AvaII GGWCC 2 cut(s) 454, 517
BamHI GGATCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 277
BauI CACGAG 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 1 cut(s) 223
BccI CCATC 1 cut(s) 5
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BcoDI GTCTC 1 cut(s) 315
BfaI CTAG 2 cut(s) 347, 390
BfrI CTTAAG 1 cut(s) 247
BisI GCNGC 1 cut(s) 212
BlsI GCNGC 1 cut(s) 213
Bme18I GGWCC 2 cut(s) 454, 517
BmgT120I GGNCC 2 cut(s) 454, 517
BmiI GGNNCC 3 cut(s) 153, 187, 456
BmsI GCATC 2 cut(s) 283, 600
BoxI GACNNNNGTC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 553
BsaAI YACGTR 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 1 cut(s) 484
BseNI ACTGG 1 cut(s) 469
BseXI GCAGC 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 277
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 5 cut(s) 185, 392, 563, 619, 631
BspLI GGNNCC 3 cut(s) 153, 187, 456
BspPI GGATC 2 cut(s) 180, 193
BspTI CTTAAG 1 cut(s) 247
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 5 cut(s) 185, 392, 563, 619, 631
BssSI CACGAG 1 cut(s) 215
Bst2BI CACGAG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 69
BstAFI CTTAAG 1 cut(s) 247
BstAPI GCANNNNNTGC 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 234
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 622
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 5 cut(s) 188, 395, 566, 622, 634
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 5 cut(s) 185, 392, 563, 619, 631
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstPAI GACNNNNGTC 1 cut(s) 576
BstV1I GCAGC 1 cut(s) 223
BstX2I RGATCY 1 cut(s) 185
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 1 cut(s) 185
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 622
CfoI GCGC 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 454, 517
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviAII CATG 2 cut(s) 109, 206
CviJI RGCY 4 cut(s) 275, 477, 507, 625
CviKI_1 RGCY 4 cut(s) 275, 477, 507, 625
DpnI GATC 5 cut(s) 187, 394, 565, 621, 633
DpnII GATC 5 cut(s) 185, 392, 563, 619, 631
Ecl136II GAGCTC 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 277
Eco47I GGWCC 2 cut(s) 454, 517
Eco53kI GAGCTC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 277
FaeI CATG 2 cut(s) 112, 209
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 1 cut(s) 451
FatI CATG 2 cut(s) 108, 205
FblI GTMKAC 2 cut(s) 38, 58
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 1 cut(s) 629
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 1 cut(s) 212
FspBI CTAG 2 cut(s) 347, 390
GlaI GCGC 1 cut(s) 245
GluI GCNGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 246
Hin1II CATG 2 cut(s) 112, 209
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HinfI GANTC 2 cut(s) 18, 583
Hpy166II GTNNAC 4 cut(s) 39, 49, 59, 539
Hpy188I TCNGA 2 cut(s) 180, 582
Hpy188III TCNNGA 2 cut(s) 390, 532
Hpy8I GTNNAC 4 cut(s) 39, 49, 59, 539
HpyAV CCTTC 2 cut(s) 47, 569
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 5 cut(s) 26, 269, 296, 590, 613
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 2 cut(s) 112, 209
HspAI GCGC 1 cut(s) 244
Kzo9I GATC 5 cut(s) 185, 392, 563, 619, 631
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 4 cut(s) 255, 471, 482, 599
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 2 cut(s) 283, 600
MaeI CTAG 2 cut(s) 347, 390
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 446, 556
MalI GATC 5 cut(s) 187, 394, 565, 621, 633
MboI GATC 5 cut(s) 185, 392, 563, 619, 631
MboII GAAGA 1 cut(s) 27
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 1 cut(s) 277
MluCI AATT 5 cut(s) 239, 264, 303, 369, 479
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 1 cut(s) 560
MnlI CCTC 4 cut(s) 73, 142, 165, 211
MseI TTAA 3 cut(s) 120, 248, 525
MslI CAYNNNNRTG 3 cut(s) 111, 113, 232
MspCI CTTAAG 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 371
NdeII GATC 5 cut(s) 185, 392, 563, 619, 631
NlaIII CATG 2 cut(s) 112, 209
NlaIV GGNNCC 3 cut(s) 153, 187, 456
NmuCI GTSAC 1 cut(s) 556
OliI CACNNNNGTG 1 cut(s) 232
PfeI GAWTC 1 cut(s) 18
PkrI GCNGC 1 cut(s) 213
PleI GAGTC 1 cut(s) 591
PpsI GAGTC 1 cut(s) 591
Ppu21I YACGTR 1 cut(s) 234
PshAI GACNNNNGTC 1 cut(s) 576
PsiI TTATAA 1 cut(s) 74
Psp124BI GAGCTC 1 cut(s) 277
PspN4I GGNNCC 3 cut(s) 153, 187, 456
PspPI GGNCC 2 cut(s) 454, 517
PsuI RGATCY 1 cut(s) 185
RseI CAYNNNNRTG 3 cut(s) 111, 113, 232
SacI GAGCTC 1 cut(s) 277
SaqAI TTAA 3 cut(s) 120, 248, 525
SatI GCNGC 1 cut(s) 212
Sau3AI GATC 5 cut(s) 185, 392, 563, 619, 631
Sau96I GGNCC 2 cut(s) 454, 517
SchI GAGTC 1 cut(s) 592
SduI GDGCHC 1 cut(s) 277
SetI ASST 8 cut(s) 38, 58, 84, 153, 236, 277, 509, 580
SfaNI GCATC 2 cut(s) 283, 600
SinI GGWCC 2 cut(s) 454, 517
SmiMI CAYNNNNRTG 3 cut(s) 111, 113, 232
SmlI CTYRAG 2 cut(s) 247, 532
SmoI CTYRAG 2 cut(s) 247, 532
Sse9I AATT 5 cut(s) 239, 264, 303, 369, 479
SspMI CTAG 2 cut(s) 347, 390
SstI GAGCTC 1 cut(s) 277
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 1 cut(s) 236
TaqI TCGA 2 cut(s) 473, 634
TasI AATT 5 cut(s) 239, 264, 303, 369, 479
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 3 cut(s) 120, 248, 525
Tru9I TTAA 3 cut(s) 120, 248, 525
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 4 cut(s) 125, 305, 366, 589
TspGWI ACGGA 2 cut(s) 275, 500
Vha464I CTTAAG 1 cut(s) 247
VpaK11BI GGWCC 2 cut(s) 454, 517
XbaI TCTAGA 1 cut(s) 389
XmiI GTMKAC 2 cut(s) 38, 58
XspI CTAG 2 cut(s) 347, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.