Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0006784

Physical Location & Seq

Genomic Coordinates Reverse (-)

53665128 .. 53668052

Gene Structure 2925 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0364400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGCCTCCTCTCCTTTCCTCTCCAAGCCCACCATTTCATTCTCCTCCTCCGTCTCCACCTCCCTCCCATGGCGCCCCTCCCTCTCCTCCACCGCATTTCGCGCCAACCCCCGCCGCAGAACCCTGACCGTCCAATCCAAGATCAGGGAGATCTTCATGCCCGCCCTCAGCTCCACCATGACCGAGGGCAAGATCGTCTCCTGGATCAAATCCGAGGGCGACGTCCTCTCCAAGGGCGAGAGCGTCGTCGTTGTAGAGTCCGACAAGGCTGATATGGACGTCGAGACTTTCTACGACGGCATCCTCGCCGCGATTGTCGTCGGCGAGGGCGAGACCGCCCCCGTCGGCGCCGCCATCGGAATTCTCGCCGAGACTGAAGCCGAGCTCGATGAGGCCAAGGCTAAAGCCAAAGCCGCCGATTCCGGTGCCGTTACGCCCACTCCTCCTCCTGCTACTGCCACTCCCGCACCTGCTATTGCGCAGCCTCCTCCTGCTGCCACGTCAGCGGCTCCAGCTGGGCCCAGGAAGACTGTGGCTACTCCCTTCGCGAAGAAGCTAGCTAAGCAACACAAGGTGGACATTGCCACCGTCGTGGGCACCGGCCCATTCGGCAGAATTACTCCTGCTGACGTGGAGGCTGCCGCCGGAATTGAGCCGAAGAAAACCGTGGCTGCCGTGCCTACTCCGGCTGCGGCGGCTCCGGCCAAGTCTGCTGCTTCTTCCCCTCCTGCATTGCCTCTACTTCCAGGTTCCACTGTCGTGCCTTTCACCACAATGCAGGCTGCAGTCTCGAAGAACATGATCGAAAGTCTCTCGGTGCCGACGTTCCGGGTGGGCTATCCGGTGAACAGCGATGCCATAGATGCGATGTATGATAAGGTGAAACGAAAGGGCGTGACCATGACTGCCTTGCTTGCCAAGGCGGTTGCTATGGCACTTGCTCAGCACCCGGTTGTGAATGCCAGCTGCAAAGATGGGAAGAGCTTTTACTACAACAGTAGCATAAACATCGCCGTTGCTGTGGCTATTGATGGTGGGCTGATAACCCCTGTTCTTCAGGATGCAGATAAGGTGGATTTGTATTTGTTATCTCAGAAGTGGAAAGAGCTGGTGGAGAAGGCTCGCTCAAAGCAACTTCAGCCCCATGAGTACAACTCAGGAACTTTCACTCTCTCCAATTTGGGAATGTTTGGAGTGGACAGATTCGATGCTATTCTTCCTCCAGGCCAGGGTGCTATCATGGCTGTTGGAGCTTCAAAACCCACTGTTGTAGCTGATGCTGACGGATTCTTCAGTGTAAAAAGTAAAATGCTGGTGAATGTAACAGCTGATCACCGAATTGTTTATGGAGCTGATTTGGCTGCCTTCCTACAGACATTTGCAAAGATTGTTGAGAACCCAGAAAGCCTGACAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

AAA

Protein Analysis

472

Amino Acids

49.12

Weight (kDa)

8.64

Isoelectric Point (pI)

44.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	50 - 120	3.6e-14	Biotin-requiring enzyme
E3_binding	PF02817	178 - 212	1.6e-13	e3 binding domain
2-oxoacid_dh	PF00198	251 - 471	7.8e-64	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006784)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56690
fragaria_vesca	FvH4_6g32040 FvH4_6g32040 FvH4_6g32040 FvH4_6g32040 FvH4_6g32040
malus_domestica	MD09G1206200.v1.1
prunus_persica	Prupe.3G062600_v2.0.a1 Prupe.3G062600_v2.0.a1 Prupe.3G062600_v2.0.a1 Prupe.3G062600_v2.0.a1 Prupe.3G062600_v2.0.a1
pyrus_communis	pycom09g12300
rosa_chinensis	RchiOBHm_Chr2g0139701
rosa_laevigata	RLG00000019827
rosa_multiflora	Rmu_co8172878.1_g000001 Rmu_sc0009714.1_g000008
rosa_roxburghii	Rroxscaffold_2G00105270
rosa_rugosa	Rorug02G0359300 Rorug04G0364300 Rorug04G0364400
rosa_samantha	Rh2AG397600 Rh2AG409100 Rh2BG419400 Rh4CG452300
rosa_wichuraiana	Rw2G033460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	478
AatII	GACGTC	2 cut(s)	225, 282
Acc16I	TGCGCA	1 cut(s)	480
Acc36I	ACCTGC	1 cut(s)	478
AccB1I	GGYRCC	5 cut(s)	72, 347, 425, 596, 817
AccII	CGCG	3 cut(s)	102, 311, 548
AclWI	GGATC	1 cut(s)	212
AcoI	YGGCCR	1 cut(s)	702
AcsI	RAATTY	1 cut(s)	360
AcuI	CTGAAG	4 cut(s)	396, 1040, 1121, 1276
AcyI	GRCGYC	4 cut(s)	73, 222, 279, 348
AdeI	CACNNNGTG	1 cut(s)	574
AfaI	GTAC	1 cut(s)	1151
AfiI	CCNNNNNNNGG	4 cut(s)	69, 144, 232, 1229
AgsI	TTSAA	1 cut(s)	1257
AjiI	CACGTC	2 cut(s)	501, 631
AjnI	CCWGG	5 cut(s)	200, 521, 745, 1222, 1227
AleI	CACNNNNGTG	2 cut(s)	590, 758
Alw21I	GWGCWC	1 cut(s)	387
Alw26I	GTCTC	7 cut(s)	58, 202, 278, 326, 365, 793, 815
AlwI	GGATC	1 cut(s)	212
AoxI	GGCC	6 cut(s)	3, 393, 518, 601, 702, 1225
ApaI	GGGCCC	1 cut(s)	522
ApeKI	GCWGC	9 cut(s)	481, 494, 638, 671, 689, 713, 782, 966, 1361
ApoI	RAATTY	1 cut(s)	360
AspLEI	GCGC	4 cut(s)	75, 104, 350, 481
AspS9I	GGNCC	3 cut(s)	518, 519, 602
AsuC2I	CCSGG	2 cut(s)	830, 950
AsuHPI	GGTGA	5 cut(s)	760, 856, 892, 1325, 1327
AsuNHI	GCTAGC	1 cut(s)	556
BaeGI	GKGCMC	2 cut(s)	522, 599
BanI	GGYRCC	5 cut(s)	72, 347, 425, 596, 817
BanII	GRGCYC	2 cut(s)	387, 522
BarI	GAAGNNNNNNTAC	2 cut(s)	971, 1003
BbsI	GAAGAC	1 cut(s)	533
Bbv12I	GWGCWC	1 cut(s)	387
BbvCI	CCTCAGC	1 cut(s)	167
BbvI	GCAGC	9 cut(s)	481, 493, 625, 658, 676, 700, 769, 953, 1348
BccI	CCATC	3 cut(s)	362, 968, 1025
BceAI	ACGGC	4 cut(s)	313, 413, 659, 998
BcgI	CGANNNNNNTGC	4 cut(s)	407, 441, 991, 1025
BciT130I	CCWGG	5 cut(s)	202, 523, 747, 1224, 1229
BclI	TGATCA	1 cut(s)	1330
BcnI	CCSGG	2 cut(s)	830, 950
BcoDI	GTCTC	7 cut(s)	58, 202, 278, 326, 365, 793, 815
BfaI	CTAG	1 cut(s)	557
BfmI	CTRYAG	2 cut(s)	783, 1370
BfoI	RGCGCY	2 cut(s)	76, 351
BfuAI	ACCTGC	1 cut(s)	478
BglI	GCCNNNNNGGC	1 cut(s)	609
BglII	AGATCT	1 cut(s)	150
BlpI	GCTNAGC	2 cut(s)	561, 942
Bme1390I	CCNGG	7 cut(s)	202, 523, 747, 830, 950, 1224, 1229
BmgBI	CACGTC	2 cut(s)	501, 631
BmgT120I	GGNCC	3 cut(s)	518, 519, 602
BmiI	GGNNCC	9 cut(s)	74, 349, 427, 510, 520, 598, 699, 751, 819
BmrFI	CCNGG	7 cut(s)	202, 523, 747, 830, 950, 1224, 1229
BmsI	GCATC	6 cut(s)	309, 844, 853, 1051, 1198, 1267
BmtI	GCTAGC	1 cut(s)	560
BpiI	GAAGAC	1 cut(s)	533
BplI	GAGNNNNNCTC	2 cut(s)	1139, 1171
BpmI	CTGGAG	2 cut(s)	495, 1206
Bpu10I	CCTNAGC	1 cut(s)	167
Bpu1102I	GCTNAGC	2 cut(s)	561, 942
BpuMI	CCSGG	2 cut(s)	830, 950
BsaHI	GRCGYC	4 cut(s)	73, 222, 279, 348
BsaI	GGTCTC	1 cut(s)	326
BsaJI	CCNNGG	9 cut(s)	68, 183, 213, 231, 396, 521, 666, 918, 1228
BsaWI	WCCGGW	2 cut(s)	422, 841
BsaXI	ACNNNNNCTCC	8 cut(s)	212, 242, 424, 430, 445, 454, 460, 475
Bsc4I	CCNNNNNNNGG	4 cut(s)	69, 144, 232, 1229
Bse118I	RCCGGY	1 cut(s)	599
Bse3DI	GCAATG	2 cut(s)	579, 731
BseBI	CCWGG	5 cut(s)	202, 523, 747, 1224, 1229
BseDI	CCNNGG	9 cut(s)	68, 183, 213, 231, 396, 521, 666, 918, 1228
BseGI	GGATG	2 cut(s)	300, 1066
BseLI	CCNNNNNNNGG	4 cut(s)	69, 144, 232, 1229
BseMI	GCAATG	2 cut(s)	579, 731
BseMII	CTCAG	4 cut(s)	181, 956, 1106, 1170
BseRI	GAGGAG	6 cut(s)	34, 37, 76, 432, 435, 477
BseSI	GKGCMC	2 cut(s)	522, 599
BseXI	GCAGC	9 cut(s)	481, 493, 625, 658, 676, 700, 769, 953, 1348
BseYI	CCCAGC	1 cut(s)	515
Bsh1236I	CGCG	3 cut(s)	102, 311, 548
BshFI	GGCC	6 cut(s)	5, 395, 520, 603, 704, 1227
BshNI	GGYRCC	5 cut(s)	72, 347, 425, 596, 817
BsiHKAI	GWGCWC	1 cut(s)	387
BsiSI	CCGG	8 cut(s)	423, 600, 645, 686, 701, 829, 842, 950
BslI	CCNNNNNNNGG	4 cut(s)	69, 144, 232, 1229
BsmAI	GTCTC	7 cut(s)	58, 202, 278, 326, 365, 793, 815
BsmBI	CGTCTC	2 cut(s)	58, 202
BsmI	GAATGC	1 cut(s)	964
BsnI	GGCC	6 cut(s)	5, 395, 520, 603, 704, 1227
Bso31I	GGTCTC	1 cut(s)	326
Bsp120I	GGGCCC	1 cut(s)	518
Bsp1286I	GDGCHC	3 cut(s)	387, 522, 599
Bsp143I	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
Bsp1720I	GCTNAGC	2 cut(s)	561, 942
Bsp19I	CCATGG	1 cut(s)	68
Bsp68I	TCGCGA	1 cut(s)	548
BspANI	GGCC	6 cut(s)	5, 395, 520, 603, 704, 1227
BspCNI	CTCAG	4 cut(s)	180, 955, 1105, 1169
BspFNI	CGCG	3 cut(s)	102, 311, 548
BspLI	GGNNCC	9 cut(s)	74, 349, 427, 510, 520, 598, 699, 751, 819
BspMAI	CTGCAG	1 cut(s)	787
BspMI	ACCTGC	1 cut(s)	478
BspOI	GCTAGC	1 cut(s)	560
BspPI	GGATC	1 cut(s)	212
BspQI	GCTCTTC	1 cut(s)	974
BspT107I	GGYRCC	5 cut(s)	72, 347, 425, 596, 817
BspTNI	GGTCTC	1 cut(s)	326
BsrDI	GCAATG	2 cut(s)	579, 731
BsrFI	RCCGGY	1 cut(s)	599
BssAI	RCCGGY	1 cut(s)	599
BssECI	CCNNGG	9 cut(s)	68, 183, 213, 231, 396, 521, 666, 918, 1228
BssMI	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
BssNI	GRCGYC	4 cut(s)	73, 222, 279, 348
BssT1I	CCWWGG	4 cut(s)	68, 231, 396, 918
Bst2UI	CCWGG	5 cut(s)	202, 523, 747, 1224, 1229
Bst4CI	ACNGT	7 cut(s)	130, 532, 589, 667, 757, 998, 1267
Bst6I	CTCTTC	1 cut(s)	974
BstACI	GRCGYC	4 cut(s)	73, 222, 279, 348
BstC8I	GCNNGC	6 cut(s)	162, 558, 780, 915, 964, 1123
BstDEI	CTNAG	5 cut(s)	167, 561, 942, 1092, 1156
BstDSI	CCRYGG	2 cut(s)	68, 666
BstENI	CCTNNNNNAGG	1 cut(s)	230
BstF5I	GGATG	2 cut(s)	300, 1066
BstFNI	CGCG	3 cut(s)	102, 311, 548
BstH2I	RGCGCY	2 cut(s)	76, 351
BstHHI	GCGC	4 cut(s)	75, 104, 350, 481
BstKTI	GATC	6 cut(s)	144, 153, 195, 207, 804, 1333
BstMAI	GTCTC	7 cut(s)	58, 202, 278, 326, 365, 793, 815
BstMBI	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
BstNI	CCWGG	5 cut(s)	202, 523, 747, 1224, 1229
BstSCI	CCNGG	7 cut(s)	200, 521, 745, 828, 948, 1222, 1227
BstSFI	CTRYAG	2 cut(s)	783, 1370
BstSLI	GKGCMC	2 cut(s)	522, 599
BstUI	CGCG	3 cut(s)	102, 311, 548
BstV1I	GCAGC	9 cut(s)	481, 493, 625, 658, 676, 700, 769, 953, 1348
BstV2I	GAAGAC	1 cut(s)	533
BstX2I	RGATCY	1 cut(s)	150
BstXI	CCANNNNNNTGG	1 cut(s)	592
BstYI	RGATCY	1 cut(s)	150
BsuRI	GGCC	6 cut(s)	5, 395, 520, 603, 704, 1227
BtgI	CCRYGG	2 cut(s)	68, 666
BtgZI	GCGATG	3 cut(s)	867, 881, 994
BtrI	CACGTC	2 cut(s)	501, 631
BtsCI	GGATG	2 cut(s)	300, 1066
BtsIMutI	CAGTG	3 cut(s)	753, 1263, 1300
BtuMI	TCGCGA	1 cut(s)	548
BveI	ACCTGC	1 cut(s)	478
Cac8I	GCNNGC	6 cut(s)	162, 558, 780, 915, 964, 1123
CfoI	GCGC	4 cut(s)	75, 104, 350, 481
Cfr10I	RCCGGY	1 cut(s)	599
Cfr13I	GGNCC	3 cut(s)	518, 519, 602
CseI	GACGC	1 cut(s)	232
Csp6I	GTAC	1 cut(s)	1150
CviAII	CATG	7 cut(s)	69, 157, 178, 799, 901, 1145, 1240
CviQI	GTAC	1 cut(s)	1150
DdeI	CTNAG	5 cut(s)	167, 561, 942, 1092, 1156
DinI	GGCGCC	2 cut(s)	74, 349
DpnI	GATC	6 cut(s)	143, 152, 194, 206, 803, 1332
DpnII	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
DraIII	CACNNNGTG	1 cut(s)	574
EaeI	YGGCCR	1 cut(s)	702
Eam1104I	CTCTTC	1 cut(s)	974
EarI	CTCTTC	1 cut(s)	974
Ecl136II	GAGCTC	1 cut(s)	385
Eco130I	CCWWGG	4 cut(s)	68, 231, 396, 918
Eco24I	GRGCYC	2 cut(s)	387, 522
Eco31I	GGTCTC	1 cut(s)	326
Eco53kI	GAGCTC	1 cut(s)	385
Eco57I	CTGAAG	4 cut(s)	396, 1040, 1121, 1276
EcoICRI	GAGCTC	1 cut(s)	385
EcoNI	CCTNNNNNAGG	1 cut(s)	230
EcoRI	GAATTC	1 cut(s)	360
EcoRII	CCWGG	5 cut(s)	200, 521, 745, 1222, 1227
EcoT14I	CCWWGG	4 cut(s)	68, 231, 396, 918
EcoT38I	GRGCYC	2 cut(s)	387, 522
EgeI	GGCGCC	2 cut(s)	74, 349
EheI	GGCGCC	2 cut(s)	74, 349
ErhI	CCWWGG	4 cut(s)	68, 231, 396, 918
Esp3I	CGTCTC	2 cut(s)	58, 202
FaeI	CATG	7 cut(s)	72, 160, 181, 802, 904, 1148, 1243
FatI	CATG	7 cut(s)	68, 156, 177, 798, 900, 1144, 1239
FauI	CCCGC	3 cut(s)	119, 169, 472
FbaI	TGATCA	1 cut(s)	1330
FokI	GGATG	2 cut(s)	287, 1073
FriOI	GRGCYC	2 cut(s)	387, 522
FspBI	CTAG	1 cut(s)	557
FspI	TGCGCA	1 cut(s)	480
GlaI	GCGC	4 cut(s)	74, 103, 349, 480
GsaI	CCCAGC	1 cut(s)	519
GsuI	CTGGAG	2 cut(s)	495, 1206
HaeII	RGCGCY	2 cut(s)	76, 351
HaeIII	GGCC	6 cut(s)	5, 395, 520, 603, 704, 1227
HapII	CCGG	8 cut(s)	423, 600, 645, 686, 701, 829, 842, 950
HgaI	GACGC	1 cut(s)	232
HhaI	GCGC	4 cut(s)	75, 104, 350, 481
Hin1I	GRCGYC	4 cut(s)	73, 222, 279, 348
Hin1II	CATG	7 cut(s)	72, 160, 181, 802, 904, 1148, 1243
Hin6I	GCGC	4 cut(s)	73, 102, 348, 479
HinP1I	GCGC	4 cut(s)	73, 102, 348, 479
HinfI	GANTC	4 cut(s)	257, 419, 1203, 1287
HpaII	CCGG	8 cut(s)	423, 600, 645, 686, 701, 829, 842, 950
HphI	GGTGA	5 cut(s)	760, 856, 892, 1325, 1327
Hpy166II	GTNNAC	3 cut(s)	577, 847, 1198
Hpy188I	TCNGA	4 cut(s)	214, 262, 359, 1095
Hpy188III	TCNNGA	5 cut(s)	283, 547, 790, 1058, 1158
Hpy8I	GTNNAC	3 cut(s)	577, 847, 1198
Hpy99I	CGWCG	9 cut(s)	224, 248, 251, 284, 299, 323, 347, 593, 826
HpyAV	CCTTC	3 cut(s)	553, 1111, 1375
HpyCH4III	ACNGT	7 cut(s)	130, 532, 589, 667, 757, 998, 1267
HpyCH4IV	ACGT	5 cut(s)	222, 279, 500, 630, 824
HpyCH4V	TGCA	6 cut(s)	731, 778, 785, 969, 1064, 1382
HpyF3I	CTNAG	5 cut(s)	167, 561, 942, 1092, 1156
HpySE526I	ACGT	5 cut(s)	222, 279, 500, 630, 824
Hsp92I	GRCGYC	4 cut(s)	73, 222, 279, 348
Hsp92II	CATG	7 cut(s)	72, 160, 181, 802, 904, 1148, 1243
HspAI	GCGC	4 cut(s)	73, 102, 348, 479
KasI	GGCGCC	2 cut(s)	72, 347
Ksp22I	TGATCA	1 cut(s)	1330
Kzo9I	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
LguI	GCTCTTC	1 cut(s)	974
LmnI	GCTCC	5 cut(s)	176, 514, 703, 1250, 1349
Lsp1109I	GCAGC	9 cut(s)	481, 493, 625, 658, 676, 700, 769, 953, 1348
LweI	GCATC	6 cut(s)	309, 844, 853, 1051, 1198, 1267
MaeI	CTAG	1 cut(s)	557
MaeII	ACGT	5 cut(s)	222, 279, 500, 630, 824
MaeIII	GTNAC	3 cut(s)	430, 895, 1321
MalI	GATC	6 cut(s)	143, 152, 194, 206, 803, 1332
MboI	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
MflI	RGATCY	1 cut(s)	150
MhlI	GDGCHC	3 cut(s)	387, 522, 599
MluCI	AATT	5 cut(s)	360, 615, 648, 1177, 1338
Mly113I	GGCGCC	2 cut(s)	73, 348
MlyI	GAGTC	1 cut(s)	266
MmeI	TCCRAC	2 cut(s)	285, 1228
MslI	CAYNNNNRTG	3 cut(s)	590, 758, 773
MspA1I	CMGCKG	4 cut(s)	506, 515, 966, 1328
MspI	CCGG	8 cut(s)	423, 600, 645, 686, 701, 829, 842, 950
MspR9I	CCNGG	7 cut(s)	202, 523, 747, 830, 950, 1224, 1229
Mva1269I	GAATGC	1 cut(s)	964
MvaI	CCWGG	5 cut(s)	202, 523, 747, 1224, 1229
MvnI	CGCG	3 cut(s)	102, 311, 548
NarI	GGCGCC	2 cut(s)	73, 348
NciI	CCSGG	2 cut(s)	830, 950
NcoI	CCATGG	1 cut(s)	68
NdeII	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
NheI	GCTAGC	1 cut(s)	556
NlaIII	CATG	7 cut(s)	72, 160, 181, 802, 904, 1148, 1243
NlaIV	GGNNCC	9 cut(s)	74, 349, 427, 510, 520, 598, 699, 751, 819
NmeAIII	GCCGAG	2 cut(s)	394, 406
NmuCI	GTSAC	1 cut(s)	895
NruI	TCGCGA	1 cut(s)	548
NsbI	TGCGCA	1 cut(s)	480
OliI	CACNNNNGTG	2 cut(s)	590, 758
PaqCI	CACCTGC	1 cut(s)	478
PciSI	GCTCTTC	1 cut(s)	974
PcsI	WCGNNNNNNNCGW	2 cut(s)	327, 821
PctI	GAATGC	1 cut(s)	964
PfeI	GAWTC	3 cut(s)	419, 1203, 1287
PfoI	TCCNGGA	1 cut(s)	200
PleI	GAGTC	1 cut(s)	265
PluTI	GGCGCC	2 cut(s)	76, 351
PpsI	GAGTC	1 cut(s)	265
Psp124BI	GAGCTC	1 cut(s)	387
Psp6I	CCWGG	5 cut(s)	200, 521, 745, 1222, 1227
PspFI	CCCAGC	1 cut(s)	515
PspGI	CCWGG	5 cut(s)	200, 521, 745, 1222, 1227
PspN4I	GGNNCC	9 cut(s)	74, 349, 427, 510, 520, 598, 699, 751, 819
PspOMI	GGGCCC	1 cut(s)	518
PspPI	GGNCC	3 cut(s)	518, 519, 602
PstI	CTGCAG	1 cut(s)	787
PsuI	RGATCY	1 cut(s)	150
PvuII	CAGCTG	3 cut(s)	515, 966, 1328
RruI	TCGCGA	1 cut(s)	548
RsaI	GTAC	1 cut(s)	1151
RsaNI	GTAC	1 cut(s)	1150
RseI	CAYNNNNRTG	3 cut(s)	590, 758, 773
SacI	GAGCTC	1 cut(s)	387
SapI	GCTCTTC	1 cut(s)	974
Sau3AI	GATC	6 cut(s)	141, 150, 192, 204, 801, 1330
Sau96I	GGNCC	3 cut(s)	518, 519, 602
SchI	GAGTC	1 cut(s)	266
ScrFI	CCNGG	7 cut(s)	202, 523, 747, 830, 950, 1224, 1229
SduI	GDGCHC	3 cut(s)	387, 522, 599
SfaNI	GCATC	6 cut(s)	309, 844, 853, 1051, 1198, 1267
SfcI	CTRYAG	2 cut(s)	783, 1370
SfoI	GGCGCC	2 cut(s)	74, 349
SmiMI	CAYNNNNRTG	3 cut(s)	590, 758, 773
Sse9I	AATT	5 cut(s)	360, 615, 648, 1177, 1338
SspDI	GGCGCC	2 cut(s)	72, 347
SspMI	CTAG	1 cut(s)	557
SstI	GAGCTC	1 cut(s)	387
StyD4I	CCNGG	7 cut(s)	200, 521, 745, 828, 948, 1222, 1227
StyI	CCWWGG	4 cut(s)	68, 231, 396, 918
TaaI	ACNGT	7 cut(s)	130, 532, 589, 667, 757, 998, 1267
TaiI	ACGT	5 cut(s)	225, 282, 503, 633, 827
TaqI	TCGA	5 cut(s)	282, 387, 791, 804, 1206
TaqII	GACCGA	1 cut(s)	197
TasI	AATT	5 cut(s)	360, 615, 648, 1177, 1338
TatI	WGTACW	1 cut(s)	1149
TauI	GCSGC	8 cut(s)	117, 311, 353, 416, 509, 644, 695, 698
TfiI	GAWTC	3 cut(s)	419, 1203, 1287
TscAI	CASTG	3 cut(s)	760, 1270, 1300
TseFI	GTSAC	1 cut(s)	895
TseI	GCWGC	9 cut(s)	481, 494, 638, 671, 689, 713, 782, 966, 1361
Tsp45I	GTSAC	1 cut(s)	895
TspDTI	ATGAA	2 cut(s)	27, 145
TspGWI	ACGGA	2 cut(s)	40, 1299
TspRI	CASTG	3 cut(s)	760, 1270, 1300
XagI	CCTNNNNNAGG	1 cut(s)	230
XapI	RAATTY	1 cut(s)	360
XcmI	CCANNNNNNNNNTGG	1 cut(s)	529
XspI	CTAG	1 cut(s)	557
ZraI	GACGTC	2 cut(s)	223, 280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.