Rorug04G0397100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012667
Physical Location & Seq
Forward (+)
56328559 .. 56331788
3230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0397100.1

Sequence Viewer

Length: 915 bp
ATGACACTCCGACCTCACACTCTCTTCCTCCCTCAAGCCTCCTTAACCCCCCCATCTCCTAAACCCTCCAAAACCCCTTCCAACTCCTTCACCCCAACTCTCAATTCCCCAATCTACCCCTCCCTCTCCCTCCACCACCACCACCCAACCGACTCCGGCATCCTCTTCCGCGAAAAGCTCATCTACCTCGAAACCCACCTCAACGTCAATCCCCAAAAAGCCCTCCATCTAAACCCCGACTTCCGCTCCTGCCCCCTCTCCTCCGTCAAGTCCGTCGAGCACTGCCTCTCCTCCATGGGCATCGAGCTCTCCGCCATGGGCCGCATCCTCGACATGCACCCCCAGCTCCTCACCGTGGACCCCCACTGCCACCTCTACCCCGTCTTCGACTTCCTCCTCCACGACGCCCATATCCCCTTCCACGATATCCGCAAGTCCATCATCCGCTGCCCCAGGCTCCTCGTCTGTGACGTCAGCAACCAACTCCGACCCGCCTTGGAGTTCCTGACCCACCAACTAGGTTTTAAGGGCGTGACGTGCCAGACCACGGTCCTATTGGTCTCCAGCGTTAGAGGTACCCTTTCGCCCAAGATTGAGTATTTGCAGAGTCTGGGGCTGAGTCACGCCGAGGTGGCCAACATGGTGATAAGGTCGCCGGCGCTGTTGACTTTAAGCATACCCAACAATTTCATGCCGAAAGTCGAGTATTTAGTGCAAGAGATGAAGAAAGATGTGAAGGAGCTCAAGAGGTTTCCTCAGTACTTTTCGTTTAGTTTGGAGGGGAAGATTAAGCCTAGGCACAGATTGTTGGCTGAGCACGGCTTTTCGCTTCCGTTGAGGGACATGTTGAGGGTTAGTGATGGCGAGTTTAATGCTATGTTGATTGAGATGCGATTGGATTGCAGAGGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

304

Amino Acids

34.5

Weight (kDa)

8.95

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 96 - 290 8.9e-34 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 474
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 575
AccBSI CCGCTC 1 cut(s) 246
AccII CGCG 1 cut(s) 171
AciI CCGC 7 cut(s) 169, 244, 312, 322, 430, 445, 492
AcoI YGGCCR 1 cut(s) 633
AcyI GRCGYC 2 cut(s) 405, 471
AfaI GTAC 2 cut(s) 577, 761
AfiI CCNNNNNNNGG 2 cut(s) 355, 547
AflIII ACRYGT 1 cut(s) 843
AjiI CACGTC 1 cut(s) 537
AjnI CCWGG 1 cut(s) 452
AjuI GAANNNNNNNTTGG 2 cut(s) 88, 120
AluBI AGCT 4 cut(s) 178, 307, 346, 742
AluI AGCT 4 cut(s) 178, 307, 346, 742
Alw21I GWGCWC 4 cut(s) 282, 309, 744, 819
Alw26I GTCTC 1 cut(s) 565
AlwNI CAGNNNCTG 1 cut(s) 610
AoxI GGCC 2 cut(s) 319, 633
ApeKI GCWGC 1 cut(s) 447
Asp718I GGTACC 1 cut(s) 575
AspA2I CCTAGG 1 cut(s) 794
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 3 cut(s) 319, 358, 550
AsuHPI GGTGA 3 cut(s) 82, 343, 655
AvaII GGWCC 2 cut(s) 358, 550
AvrII CCTAGG 1 cut(s) 794
BalI TGGCCA 1 cut(s) 635
BanI GGYRCC 1 cut(s) 575
BanII GRGCYC 2 cut(s) 309, 744
BbsI GAAGAC 1 cut(s) 376
Bbv12I GWGCWC 4 cut(s) 282, 309, 744, 819
BbvI GCAGC 1 cut(s) 434
BccI CCATC 4 cut(s) 61, 234, 446, 854
BceAI ACGGC 1 cut(s) 835
BcgI CGANNNNNNTGC 3 cut(s) 854, 882, 888
BciT130I CCWGG 1 cut(s) 454
BcoDI GTCTC 1 cut(s) 565
BfaI CTAG 3 cut(s) 518, 795, 913
BfoI RGCGCY 1 cut(s) 662
BglI GCCNNNNNGGC 1 cut(s) 632
BisI GCNGC 2 cut(s) 322, 448
BlnI CCTAGG 1 cut(s) 794
BlpI GCTNAGC 1 cut(s) 813
BlsI GCNGC 2 cut(s) 323, 449
BmcAI AGTACT 1 cut(s) 761
Bme1390I CCNGG 1 cut(s) 454
Bme18I GGWCC 2 cut(s) 358, 550
BmgBI CACGTC 1 cut(s) 537
BmgT120I GGNCC 3 cut(s) 319, 358, 550
BmiI GGNNCC 3 cut(s) 360, 458, 577
BmrFI CCNGG 1 cut(s) 454
BmsI GCATC 4 cut(s) 168, 309, 333, 879
BoxI GACNNNNGTC 1 cut(s) 548
BpiI GAAGAC 1 cut(s) 376
BplI GAGNNNNNCTC 2 cut(s) 739, 771
BpmI CTGGAG 1 cut(s) 547
Bpu1102I GCTNAGC 1 cut(s) 813
BpuEI CTTGAG 2 cut(s) 18, 728
BsaHI GRCGYC 2 cut(s) 405, 471
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 8 cut(s) 294, 315, 354, 452, 495, 546, 627, 794
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 272, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 547
Bse118I RCCGGY 1 cut(s) 655
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 8 cut(s) 294, 315, 354, 452, 495, 546, 627, 794
BseGI GGATG 3 cut(s) 159, 324, 441
BseLI CCNNNNNNNGG 2 cut(s) 355, 547
BseMII CTCAG 3 cut(s) 608, 770, 804
BseRI GAGGAG 5 cut(s) 250, 280, 338, 386, 449
BseXI GCAGC 1 cut(s) 434
BseYI CCCAGC 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 321, 635
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 4 cut(s) 282, 309, 744, 819
BsiSI CCGG 2 cut(s) 156, 656
BslFI GGGAC 1 cut(s) 854
BslI CCNNNNNNNGG 2 cut(s) 355, 547
BsmAI GTCTC 1 cut(s) 565
BsmFI GGGAC 1 cut(s) 854
BsnI GGCC 2 cut(s) 321, 635
Bso31I GGTCTC 1 cut(s) 565
Bsp1286I GDGCHC 4 cut(s) 282, 309, 744, 819
Bsp1720I GCTNAGC 1 cut(s) 813
Bsp19I CCATGG 2 cut(s) 294, 315
BspACI CCGC 7 cut(s) 169, 244, 312, 322, 430, 445, 492
BspANI GGCC 2 cut(s) 321, 635
BspCNI CTCAG 3 cut(s) 609, 769, 805
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 3 cut(s) 360, 458, 577
BspT107I GGYRCC 1 cut(s) 575
BspTNI GGTCTC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 655
BssECI CCNNGG 8 cut(s) 294, 315, 354, 452, 495, 546, 627, 794
BssNI GRCGYC 2 cut(s) 405, 471
BssT1I CCWWGG 4 cut(s) 294, 315, 495, 794
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 2 cut(s) 355, 550
Bst6I CTCTTC 2 cut(s) 29, 170
BstACI GRCGYC 2 cut(s) 405, 471
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 3 cut(s) 617, 756, 813
BstDSI CCRYGG 4 cut(s) 294, 315, 354, 546
BstF5I GGATG 3 cut(s) 159, 324, 441
BstFNI CGCG 1 cut(s) 171
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 1 cut(s) 661
BstMAI GTCTC 1 cut(s) 565
BstMWI GCNNNNNNNGC 3 cut(s) 343, 537, 632
BstNI CCWGG 1 cut(s) 454
BstNSI RCATGY 2 cut(s) 337, 847
BstPAI GACNNNNGTC 1 cut(s) 548
BstSCI CCNGG 1 cut(s) 452
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 434
BstV2I GAAGAC 1 cut(s) 376
BsuRI GGCC 2 cut(s) 321, 635
BtgI CCRYGG 4 cut(s) 294, 315, 354, 546
BtrI CACGTC 1 cut(s) 537
BtsCI GGATG 3 cut(s) 159, 324, 441
BtsI GCAGTG 2 cut(s) 280, 364
BtsIMutI CAGTG 2 cut(s) 280, 364
Cac8I GCNNGC 1 cut(s) 657
CaiI CAGNNNCTG 1 cut(s) 610
CfoI GCGC 1 cut(s) 661
Cfr10I RCCGGY 1 cut(s) 655
Cfr13I GGNCC 3 cut(s) 319, 358, 550
CseI GACGC 1 cut(s) 413
Csp6I GTAC 2 cut(s) 576, 760
CviAII CATG 6 cut(s) 295, 316, 334, 640, 691, 844
CviQI GTAC 2 cut(s) 576, 760
DdeI CTNAG 3 cut(s) 617, 756, 813
EaeI YGGCCR 1 cut(s) 633
Eam1104I CTCTTC 2 cut(s) 29, 170
EarI CTCTTC 2 cut(s) 29, 170
EciI GGCGGA 1 cut(s) 301
Ecl136II GAGCTC 2 cut(s) 307, 742
Eco130I CCWWGG 4 cut(s) 294, 315, 495, 794
Eco24I GRGCYC 2 cut(s) 309, 744
Eco31I GGTCTC 1 cut(s) 565
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 358, 550
Eco53kI GAGCTC 2 cut(s) 307, 742
EcoICRI GAGCTC 2 cut(s) 307, 742
EcoRII CCWGG 1 cut(s) 452
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 4 cut(s) 294, 315, 495, 794
EcoT38I GRGCYC 2 cut(s) 309, 744
ErhI CCWWGG 4 cut(s) 294, 315, 495, 794
FaeI CATG 6 cut(s) 298, 319, 337, 643, 694, 847
FaiI YATR 9 cut(s) 296, 317, 335, 411, 641, 677, 692, 845, 878
FaqI GGGAC 1 cut(s) 854
FatI CATG 6 cut(s) 294, 315, 333, 639, 690, 843
FauI CCCGC 1 cut(s) 499
Fnu4HI GCNGC 2 cut(s) 322, 448
FokI GGATG 3 cut(s) 146, 311, 428
FriOI GRGCYC 2 cut(s) 309, 744
Fsp4HI GCNGC 2 cut(s) 322, 448
FspBI CTAG 3 cut(s) 518, 795, 913
GlaI GCGC 1 cut(s) 660
GluI GCNGC 2 cut(s) 322, 448
GsaI CCCAGC 1 cut(s) 346
GsuI CTGGAG 1 cut(s) 547
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 2 cut(s) 321, 635
HapII CCGG 2 cut(s) 156, 656
HgaI GACGC 1 cut(s) 413
HhaI GCGC 1 cut(s) 661
Hin1I GRCGYC 2 cut(s) 405, 471
Hin1II CATG 6 cut(s) 298, 319, 337, 643, 694, 847
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HinfI GANTC 3 cut(s) 152, 607, 619
HpaII CCGG 2 cut(s) 156, 656
HphI GGTGA 3 cut(s) 82, 343, 655
Hpy166II GTNNAC 2 cut(s) 358, 666
Hpy188I TCNGA 2 cut(s) 11, 488
Hpy188III TCNNGA 2 cut(s) 505, 745
Hpy8I GTNNAC 2 cut(s) 358, 666
Hpy99I CGWCG 2 cut(s) 278, 407
HpyAV CCTTC 4 cut(s) 87, 97, 427, 730
HpyCH4III ACNGT 2 cut(s) 355, 550
HpyCH4IV ACGT 3 cut(s) 204, 471, 536
HpyCH4V TGCA 4 cut(s) 337, 604, 715, 903
HpyF10VI GCNNNNNNNGC 3 cut(s) 343, 537, 632
HpyF3I CTNAG 3 cut(s) 617, 756, 813
HpySE526I ACGT 3 cut(s) 204, 471, 536
Hsp92I GRCGYC 2 cut(s) 405, 471
Hsp92II CATG 6 cut(s) 298, 319, 337, 643, 694, 847
HspAI GCGC 1 cut(s) 659
KpnI GGTACC 1 cut(s) 579
KroI GCCGGC 1 cut(s) 655
KroNI GCCGGC 1 cut(s) 657
LmnI GCTCC 4 cut(s) 251, 351, 462, 739
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 4 cut(s) 168, 309, 333, 879
MaeI CTAG 3 cut(s) 518, 795, 913
MaeII ACGT 3 cut(s) 204, 471, 536
MaeIII GTNAC 3 cut(s) 467, 532, 620
MbiI CCGCTC 1 cut(s) 246
MboII GAAGA 5 cut(s) 16, 157, 376, 736, 796
MhlI GDGCHC 4 cut(s) 282, 309, 744, 819
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 2 cut(s) 103, 685
MluNI TGGCCA 1 cut(s) 635
MlyI GAGTC 3 cut(s) 146, 616, 628
MmeI TCCRAC 3 cut(s) 34, 105, 511
Mox20I TGGCCA 1 cut(s) 635
MreI CGCCGGCG 1 cut(s) 655
MroNI GCCGGC 1 cut(s) 655
MscI TGGCCA 1 cut(s) 635
MseI TTAA 5 cut(s) 44, 525, 671, 789, 870
Msp20I TGGCCA 1 cut(s) 635
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 2 cut(s) 156, 656
MspR9I CCNGG 1 cut(s) 454
MvaI CCWGG 1 cut(s) 454
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 343, 537, 632
NaeI GCCGGC 1 cut(s) 657
NcoI CCATGG 2 cut(s) 294, 315
NgoMIV GCCGGC 1 cut(s) 655
NlaIII CATG 6 cut(s) 298, 319, 337, 643, 694, 847
NlaIV GGNNCC 3 cut(s) 360, 458, 577
NmeAIII GCCGAG 1 cut(s) 652
NmuCI GTSAC 3 cut(s) 467, 532, 620
NspI RCATGY 2 cut(s) 337, 847
PciI ACATGT 1 cut(s) 843
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PdiI GCCGGC 1 cut(s) 657
PkrI GCNGC 2 cut(s) 323, 449
PleI GAGTC 3 cut(s) 146, 615, 627
PpsI GAGTC 3 cut(s) 146, 615, 627
PscI ACATGT 1 cut(s) 843
PshAI GACNNNNGTC 1 cut(s) 548
Psp124BI GAGCTC 2 cut(s) 309, 744
Psp6I CCWGG 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 342
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 360, 458, 577
PspPI GGNCC 3 cut(s) 319, 358, 550
PstNI CAGNNNCTG 1 cut(s) 610
RsaI GTAC 2 cut(s) 577, 761
RsaNI GTAC 2 cut(s) 576, 760
SacI GAGCTC 2 cut(s) 309, 744
SaqAI TTAA 5 cut(s) 44, 525, 671, 789, 870
SatI GCNGC 2 cut(s) 322, 448
Sau96I GGNCC 3 cut(s) 319, 358, 550
ScaI AGTACT 1 cut(s) 761
SchI GAGTC 3 cut(s) 146, 616, 628
ScrFI CCNGG 1 cut(s) 454
SduI GDGCHC 4 cut(s) 282, 309, 744, 819
SfaNI GCATC 4 cut(s) 168, 309, 333, 879
SgrAI CRCCGGYG 1 cut(s) 655
SinI GGWCC 2 cut(s) 358, 550
SmlI CTYRAG 2 cut(s) 33, 743
SmoI CTYRAG 2 cut(s) 33, 743
Sse9I AATT 2 cut(s) 103, 685
SsiI CCGC 7 cut(s) 169, 244, 312, 322, 430, 445, 492
SspMI CTAG 3 cut(s) 518, 795, 913
SstI GAGCTC 2 cut(s) 309, 744
StyD4I CCNGG 1 cut(s) 452
StyI CCWWGG 4 cut(s) 294, 315, 495, 794
TaaI ACNGT 2 cut(s) 355, 550
TaiI ACGT 3 cut(s) 207, 474, 539
TaqI TCGA 6 cut(s) 189, 276, 303, 330, 387, 702
TasI AATT 2 cut(s) 103, 685
TatI WGTACW 1 cut(s) 759
TauI GCSGC 1 cut(s) 324
Tru1I TTAA 5 cut(s) 44, 525, 671, 789, 870
Tru9I TTAA 5 cut(s) 44, 525, 671, 789, 870
TscAI CASTG 2 cut(s) 287, 371
TseFI GTSAC 3 cut(s) 467, 532, 620
TseI GCWGC 1 cut(s) 447
Tsp45I GTSAC 3 cut(s) 467, 532, 620
TspDTI ATGAA 2 cut(s) 679, 737
TspGWI ACGGA 3 cut(s) 253, 262, 822
TspRI CASTG 2 cut(s) 287, 371
VpaK11BI GGWCC 2 cut(s) 358, 550
XceI RCATGY 2 cut(s) 337, 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmaJI CCTAGG 1 cut(s) 794
XspI CTAG 3 cut(s) 518, 795, 913
ZraI GACGTC 1 cut(s) 472
ZrmI AGTACT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.