Rorug05G0203200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012666
Physical Location & Seq
Forward (+)
20202702 .. 20203220
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0203200.1

Sequence Viewer

Length: 519 bp
ATGAAGGCTTGTTCTGCGGCTGCTTTTGATAATTTGCGTGAGTTGAAGGTGGTTCTTGAGATGGACTATTTTAAGTGGAGTTTACTGACCAAGGTGCTGGGGAAATTATCTAATCTAGAGTCCCTTGTGGTGGAACATGAGAGATATGAGACGCCCTGCCATCATGGAGATTCTGAATTGGTGATTGAACTTGAAGAGTGTAAAGAAGCTACAGAAGTTTTTGCAGACGTGCTGCGTTGGAGTCCACCAGAGTTTGTGCCCAACTGTTTGCTGTCACACCTCAAGACCATCTGCATAAAGGGGTTCAAGGGAAAGGGATTCTTTGGATATCAAGATGAGAAGGAAGTGGTTAAGTACTTGTTAAAGAATGGCCAAGTTTTGCAGAGGATGGCTATTTATTCTCCTGGTCTTTCTAGGAGTGCAGAAGAGGCGTTGCATGAGGAATTTTCAACTCTTGAAAGGGATTCAAACACTTGTCAAATTGAGCTCGTCACAGAGATTGCTTGTTGGTGTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

19.65

Weight (kDa)

5.09

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 83 - 132 6.1e-15 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 17
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 443
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 7 cut(s) 46, 188, 194, 307, 450, 458, 468
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 2 cut(s) 209, 487
AluI AGCT 2 cut(s) 209, 487
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 1 cut(s) 143
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 2 cut(s) 20, 232
ApoI RAATTY 1 cut(s) 443
AsuHPI GGTGA 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 372
BanII GRGCYC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 2 cut(s) 7, 219
BccI CCATC 4 cut(s) 55, 168, 296, 382
BciT130I CCWGG 1 cut(s) 405
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 2 cut(s) 116, 414
BfmI CTRYAG 1 cut(s) 210
BisI GCNGC 3 cut(s) 18, 21, 233
BlsI GCNGC 3 cut(s) 19, 22, 234
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 1 cut(s) 405
BmgBI CACGTC 1 cut(s) 229
BmrFI CCNGG 1 cut(s) 405
BpuEI CTTGAG 2 cut(s) 77, 266
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 90
Bsc4I CCNNNNNNNGG 1 cut(s) 130
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 393
BseLI CCNNNNNNNGG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 2 cut(s) 7, 219
BseYI CCCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 441
BshFI GGCC 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 489
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 143
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 261, 489
BspACI CCGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 372
BssECI CCNNGG 1 cut(s) 90
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 2 cut(s) 189, 420
BstACI GRCGYC 1 cut(s) 152
BstF5I GGATG 1 cut(s) 393
BstMAI GTCTC 1 cut(s) 143
BstMWI GCNNNNNNNGC 2 cut(s) 14, 428
BstNI CCWGG 1 cut(s) 405
BstSCI CCNGG 1 cut(s) 403
BstSFI CTRYAG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 7, 219
BstXI CCANNNNNNTGG 1 cut(s) 97
BsuRI GGCC 1 cut(s) 372
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 393
CseI GACGC 1 cut(s) 160
Csp6I GTAC 1 cut(s) 355
CviAII CATG 3 cut(s) 137, 164, 437
CviJI RGCY 6 cut(s) 8, 20, 209, 372, 392, 487
CviKI_1 RGCY 6 cut(s) 8, 20, 209, 372, 392, 487
CviQI GTAC 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 189, 420
EarI CTCTTC 2 cut(s) 189, 420
Ecl136II GAGCTC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 329
Eco53kI GAGCTC 1 cut(s) 487
EcoICRI GAGCTC 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 403
EcoRV GATATC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 3 cut(s) 140, 167, 440
FaiI YATR 5 cut(s) 138, 147, 165, 296, 438
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FaqI GGGAC 1 cut(s) 106
FatI CATG 3 cut(s) 136, 163, 436
Fnu4HI GCNGC 3 cut(s) 18, 21, 233
FokI GGATG 1 cut(s) 400
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 3 cut(s) 18, 21, 233
FspBI CTAG 2 cut(s) 116, 414
GluI GCNGC 3 cut(s) 18, 21, 233
GsaI CCCAGC 1 cut(s) 101
HaeIII GGCC 1 cut(s) 372
HgaI GACGC 1 cut(s) 160
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 3 cut(s) 140, 167, 440
HinfI GANTC 5 cut(s) 119, 170, 241, 318, 464
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 83, 245
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 5 cut(s) 56, 116, 283, 332, 455
Hpy8I GTNNAC 2 cut(s) 83, 245
HpyAV CCTTC 2 cut(s) 40, 334
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 5 cut(s) 224, 294, 382, 422, 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 428
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 3 cut(s) 140, 167, 440
LpnPI CCDG 5 cut(s) 83, 169, 261, 390, 417
Lsp1109I GCAGC 2 cut(s) 7, 219
MaeI CTAG 2 cut(s) 116, 414
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 273, 490
MboII GAAGA 2 cut(s) 206, 437
MhlI GDGCHC 2 cut(s) 261, 489
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 5 cut(s) 31, 104, 176, 443, 480
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 2 cut(s) 128, 250
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 4 cut(s) 290, 378, 421, 433
Mox20I TGGCCA 1 cut(s) 372
MscI TGGCCA 1 cut(s) 372
MseI TTAA 3 cut(s) 72, 351, 362
Msp20I TGGCCA 1 cut(s) 372
MspR9I CCNGG 1 cut(s) 405
MvaI CCWGG 1 cut(s) 405
MwoI GCNNNNNNNGC 2 cut(s) 14, 428
NlaIII CATG 3 cut(s) 140, 167, 440
NmuCI GTSAC 2 cut(s) 273, 490
PfeI GAWTC 3 cut(s) 170, 318, 464
PkrI GCNGC 3 cut(s) 19, 22, 234
PleI GAGTC 2 cut(s) 127, 249
PpsI GAGTC 2 cut(s) 127, 249
Psp124BI GAGCTC 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 1 cut(s) 403
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SacI GAGCTC 1 cut(s) 489
SaqAI TTAA 3 cut(s) 72, 351, 362
SatI GCNGC 3 cut(s) 18, 21, 233
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 2 cut(s) 128, 250
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 2 cut(s) 261, 489
SetI ASST 6 cut(s) 51, 96, 211, 231, 282, 489
SfcI CTRYAG 1 cut(s) 210
SmlI CTYRAG 2 cut(s) 56, 281
SmoI CTYRAG 2 cut(s) 56, 281
Sse9I AATT 5 cut(s) 31, 104, 176, 443, 480
SsiI CCGC 1 cut(s) 17
SspMI CTAG 2 cut(s) 116, 414
SstI GAGCTC 1 cut(s) 489
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 1 cut(s) 231
TasI AATT 5 cut(s) 31, 104, 176, 443, 480
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 3 cut(s) 170, 318, 464
Tru1I TTAA 3 cut(s) 72, 351, 362
Tru9I TTAA 3 cut(s) 72, 351, 362
TseFI GTSAC 2 cut(s) 273, 490
TseI GCWGC 2 cut(s) 20, 232
Tsp45I GTSAC 2 cut(s) 273, 490
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 504
XapI RAATTY 1 cut(s) 443
XbaI TCTAGA 1 cut(s) 115
XspI CTAG 2 cut(s) 116, 414
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.