Rorug05G0515600

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014650
Physical Location & Seq
Reverse (-)
70028280 .. 70030709
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0515600.1

Sequence Viewer

Length: 2151 bp
ATGAGAACCATGGTAGAGCAGTCTAATCATCTATCTTCCAATTTCATCCTCTTCATTTTCTTTATCTCAACTACTCTGATTTCTGCATGCAACAAAATAGATCGAGACTCTCTTTGGTCCTCTTTTAATATGCCATCTTCTCCTTTGAATTGGTCCTCCAATGATTGCTGCAAATGGGAAGGCATCACCTGTGATACAGCTGGTCGAGTTAAACACCTATGGCTTCCCTCCAAAGGGCTCAAACTCAAAGGAAGTTTTTTCCCCTCATCATCACTTGGAAATCTCACACATCTCACACTCCTTAATCTCTCTCGCAATTCGCTTTATGGTTCACTGGATCAAACTGGATTCTTCTCATCTTTGAGTCGTCTTGAAATCCTAGATTTGAGCTATAACTTTCTTTTTGGAGAACTACCATTTTCTCTACCATCCGGTAATATGCGGAGACTGGATTTGTCCAGCAACCACTTCTATGGTTCAATTCCATCTTCATTCTTCAAACAAGCTTGGAATTTGACCAATTTTAATGTCAGTAACAACTCCTTCTCAGGTTCTATTCCATCCTCTATTTGTCTTCATTCAGTTCCCTTGATACATCTATTGGACTTTTCCTTCAATAATTTCAATGGTAGTATTTCTGGTGGGTTAGGAAAGTGTTCAAAACTTCAGATTTTTCGTGCTGCTCACAATAACCTCTCAGGAATACTCCCAAATGATATTTATAATGCTACCAAGCTCGAAGAAATTTCATTACCTCAAAATTCACTATATGGAGACATTAGTGAAAGAATTGGCAACCTCTCCTCTCTCAAAACCCTCGACCTCAACTCTAATGGATTCAATGGTATGCTCCCTATAAGTATTGGTAAGCTCTCCAAATTGAAACTCTTGCTCCTTTATTTCAACAATCTAAAAGCTTCTTTGCCCTCATCTTTGATGAATTGCACCAACCTCAAAGAATTGAGTCTGGCACAAAACAATTTGGAAGGAAATATTTCAGAGCTCAACTTCTCCAAACTTAGTCAGCTTACTAAACTAGATCTAGGGGGCAATCACCTCACTGGTTTCATTCCCAGAAGCCTTTACTCATGCAAGTCCCTGAAAGCAATTCGGATAAGCTCTAACAAGCTAGAGGGAGAAATACATCCTGATATTCTATCGTTGAAATCATTGTCGTTCTTGTCGCTTGCTTATAGTAGATTGACCAATATCACAGGGGCAATGAAGATATTGATGTATTGCAAAAGCCTAGAATTCCTTGTCTTTCCAGGTAGCTTTGTAGGTGAGGAAATGCCCGATCTTGGCATGGTTGATTTTGATGGATTCCAAAATCTTCGATTTTTATCTTTCTCTAATTGCAAGCTCACTGCTGGTCACATACCTGCATATTTATCTAAGCTCGAAAAGCTAGAGGTCTTGTATCTCCGAAACAATAGTATCACAGGGTCAATTCCTAGTTGGTTGGGGAATCTTCCAAGGCTCTATTCTATCGACTTGTCATCCAACCTACTCTCAGGTGAGTTTCCCAAGGAACTTTGTAGACTGCCGATGTTGGTATCGAAAGGAACTGCAGCTCAAGTAGAAGGTGATGAACTTGAATTATCTGTCTACTGGATTCAAGGTACAACAGTTTTACAATACAAACGGTACTTTCCTCTAGCAATAAGTATAAGCAATAATACCATTAGTGGAAGCATACCTGTTGAGATTGGTCAATTACAAGATCTCCATTACTTGGGTCTTAGCAACAATAACTTGTCAGGCAACATTCCAGACCAAATATCTAACCTGAAGAGCTTGGAGCACTTGGAACTCTCCATGAATCATTTGTCCGGAAAAATCCCATTATCATTTACAGGGCTTACTTTCTTGAACTTTTTTAATGTGTCATACAATAATCTGGAAGGTTCAATACCAGCAAGCACCCAGCTCCAAAGCTTCAATGCTTCTGCATTTGAGGGGAATACAAATCTTTGCGGTGCTCCACTTCCAAAGTGCCTACGGATCGAAGACAAGGATGCTTATAGCAGGAACAGCCGAGATGATGTAGGGAATGATGATGAAAGTCAAATTCCGTCGCTTCATATTTTCGTTACTCTTGGGTTCATTGTAGGATTTTGGGGAGTGTTTGGGCCTTTGGTGCTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

716

Amino Acids

79.14

Weight (kDa)

7.19

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 239 - 350 1.5e-09 Leucine-rich repeat region
LRR_8 PF13855 316 - 377 1.7e-08 Leucine rich repeat
LRR_8 PF13855 467 - 504 5.7e-07 Leucine rich repeat
LRR_4 PF12799 469 - 504 1.3e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 569 - 645 1e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
Acc36I ACCTGC 1 cut(s) 1390
AccB7I CCANNNNNTGG 1 cut(s) 1735
AccI GTMKAC 2 cut(s) 1540, 1608
AccIII TCCGGA 1 cut(s) 1832
AciI CCGC 2 cut(s) 442, 1977
AclWI GGATC 2 cut(s) 345, 2012
AcsI RAATTY 5 cut(s) 511, 744, 760, 1253, 2070
AcuI CTGAAG 2 cut(s) 650, 1811
AfaI GTAC 2 cut(s) 1624, 1649
AfiI CCNNNNNNNGG 4 cut(s) 233, 234, 1301, 1735
AjnI CCWGG 1 cut(s) 1267
AjuI GAANNNNNNNTTGG 2 cut(s) 1468, 1500
Alw21I GWGCWC 3 cut(s) 1005, 1806, 1984
Alw26I GTCTC 3 cut(s) 99, 439, 768
AlwI GGATC 2 cut(s) 345, 2012
Aor13HI TCCGGA 1 cut(s) 1832
AoxI GGCC 1 cut(s) 2132
ApeKI GCWGC 3 cut(s) 168, 680, 1571
ApoI RAATTY 5 cut(s) 511, 744, 760, 1253, 2070
Asp700I GAANNNNTTC 2 cut(s) 655, 994
AspS9I GGNCC 3 cut(s) 117, 153, 2132
AsuHPI GGTGA 5 cut(s) 178, 1046, 1295, 1529, 1598
AvaII GGWCC 2 cut(s) 117, 153
BaeI ACNNNNGTAYC 4 cut(s) 1420, 1453, 1614, 1647
BanII GRGCYC 2 cut(s) 240, 1005
BarI GAAGNNNNNNTAC 2 cut(s) 1896, 1928
BbsI GAAGAC 2 cut(s) 566, 2016
Bbv12I GWGCWC 3 cut(s) 1005, 1806, 1984
BbvI GCAGC 3 cut(s) 155, 667, 1583
BccI CCATC 5 cut(s) 142, 436, 493, 568, 1313
BciT130I CCWGG 1 cut(s) 1269
BcoDI GTCTC 3 cut(s) 99, 439, 768
BfaI CTAG 8 cut(s) 380, 1037, 1043, 1130, 1250, 1409, 1455, 1658
BfmI CTRYAG 1 cut(s) 1569
BfuAI ACCTGC 1 cut(s) 1390
BglII AGATCT 2 cut(s) 1039, 1723
BisI GCNGC 3 cut(s) 169, 681, 1572
BlsI GCNGC 3 cut(s) 170, 682, 1573
Bme1390I CCNGG 1 cut(s) 1269
Bme18I GGWCC 2 cut(s) 117, 153
BmgT120I GGNCC 3 cut(s) 117, 153, 2132
BmrFI CCNGG 1 cut(s) 1269
BmsI GCATC 2 cut(s) 192, 2008
BpiI GAAGAC 2 cut(s) 566, 2016
BpuEI CTTGAG 1 cut(s) 1560
BsaBI GATNNNNATC 1 cut(s) 1342
BsaJI CCNNGG 3 cut(s) 9, 1475, 1527
BsaWI WCCGGW 2 cut(s) 431, 1832
BsaXI ACNNNNNCTCC 4 cut(s) 876, 906, 1710, 1740
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 234, 1301, 1735
Bse1I ACTGG 5 cut(s) 339, 349, 453, 1066, 1616
Bse3DI GCAATG 1 cut(s) 1227
Bse8I GATNNNNATC 1 cut(s) 1342
BseAI TCCGGA 1 cut(s) 1832
BseBI CCWGG 1 cut(s) 1269
BseDI CCNNGG 3 cut(s) 9, 1475, 1527
BseGI GGATG 6 cut(s) 45, 428, 560, 1144, 1499, 2023
BseJI GATNNNNATC 1 cut(s) 1342
BseLI CCNNNNNNNGG 4 cut(s) 233, 234, 1301, 1735
BseMI GCAATG 1 cut(s) 1227
BseMII CTCAG 3 cut(s) 561, 711, 1527
BseNI ACTGG 5 cut(s) 339, 349, 453, 1066, 1616
BseRI GAGGAG 1 cut(s) 793
BseXI GCAGC 3 cut(s) 155, 667, 1583
BseYI CCCAGC 1 cut(s) 1926
BshFI GGCC 1 cut(s) 2134
BsiHKAI GWGCWC 3 cut(s) 1005, 1806, 1984
BsiSI CCGG 2 cut(s) 432, 1833
BslFI GGGAC 1 cut(s) 1081
BslI CCNNNNNNNGG 4 cut(s) 233, 234, 1301, 1735
BsmAI GTCTC 3 cut(s) 99, 439, 768
BsmFI GGGAC 1 cut(s) 1081
BsnI GGCC 1 cut(s) 2134
Bsp1286I GDGCHC 4 cut(s) 240, 1005, 1806, 1984
Bsp13I TCCGGA 1 cut(s) 1832
Bsp143I GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 2 cut(s) 442, 1977
BspANI GGCC 1 cut(s) 2134
BspCNI CTCAG 3 cut(s) 560, 710, 1526
BspEI TCCGGA 1 cut(s) 1832
BspMAI CTGCAG 1 cut(s) 1573
BspMI ACCTGC 1 cut(s) 1390
BspPI GGATC 2 cut(s) 345, 2012
BspQI GCTCTTC 1 cut(s) 1787
BsrDI GCAATG 1 cut(s) 1227
BsrI ACTGG 5 cut(s) 339, 349, 453, 1066, 1616
BssECI CCNNGG 3 cut(s) 9, 1475, 1527
BssMI GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
BssT1I CCWWGG 3 cut(s) 9, 1475, 1527
Bst2UI CCWGG 1 cut(s) 1269
Bst4CI ACNGT 2 cut(s) 1630, 1647
Bst6I CTCTTC 2 cut(s) 56, 1787
BstC8I GCNNGC 4 cut(s) 88, 1188, 1361, 1921
BstDEI CTNAG 6 cut(s) 547, 697, 1019, 1395, 1513, 1742
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 6 cut(s) 45, 428, 560, 1144, 1499, 2023
BstKTI GATC 6 cut(s) 103, 340, 1042, 1300, 1726, 2007
BstMAI GTCTC 3 cut(s) 99, 439, 768
BstMBI GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
BstMWI GCNNNNNNNGC 3 cut(s) 1405, 2034, 2140
BstNI CCWGG 1 cut(s) 1269
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 1267
BstSFI CTRYAG 1 cut(s) 1569
BstV1I GCAGC 3 cut(s) 155, 667, 1583
BstV2I GAAGAC 2 cut(s) 566, 2016
BstX2I RGATCY 2 cut(s) 1039, 1723
BstXI CCANNNNNNTGG 1 cut(s) 473
BstYI RGATCY 2 cut(s) 1039, 1723
BsuRI GGCC 1 cut(s) 2134
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 6 cut(s) 45, 428, 560, 1144, 1499, 2023
BtsI GCAGTG 1 cut(s) 1365
BtsIMutI CAGTG 3 cut(s) 332, 1059, 1365
BveI ACCTGC 1 cut(s) 1390
Cac8I GCNNGC 4 cut(s) 88, 1188, 1361, 1921
Cfr13I GGNCC 3 cut(s) 117, 153, 2132
Csp6I GTAC 2 cut(s) 1623, 1648
CviAII CATG 5 cut(s) 10, 87, 1089, 1306, 1819
CviQI GTAC 2 cut(s) 1623, 1648
DdeI CTNAG 6 cut(s) 547, 697, 1019, 1395, 1513, 1742
DpnI GATC 6 cut(s) 102, 339, 1041, 1299, 1725, 2006
DpnII GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
Eam1104I CTCTTC 2 cut(s) 56, 1787
EarI CTCTTC 2 cut(s) 56, 1787
Ecl136II GAGCTC 1 cut(s) 1003
Eco130I CCWWGG 3 cut(s) 9, 1475, 1527
Eco24I GRGCYC 2 cut(s) 240, 1005
Eco47I GGWCC 2 cut(s) 117, 153
Eco53kI GAGCTC 1 cut(s) 1003
Eco57I CTGAAG 2 cut(s) 650, 1811
EcoICRI GAGCTC 1 cut(s) 1003
EcoRI GAATTC 1 cut(s) 1253
EcoRII CCWGG 1 cut(s) 1267
EcoT14I CCWWGG 3 cut(s) 9, 1475, 1527
EcoT38I GRGCYC 2 cut(s) 240, 1005
ErhI CCWWGG 3 cut(s) 9, 1475, 1527
FaeI CATG 5 cut(s) 13, 90, 1092, 1309, 1822
FaqI GGGAC 1 cut(s) 1081
FatI CATG 5 cut(s) 9, 86, 1088, 1305, 1818
FblI GTMKAC 2 cut(s) 1540, 1608
Fnu4HI GCNGC 3 cut(s) 169, 681, 1572
FokI GGATG 6 cut(s) 32, 415, 547, 1131, 1486, 2030
FriOI GRGCYC 2 cut(s) 240, 1005
Fsp4HI GCNGC 3 cut(s) 169, 681, 1572
FspBI CTAG 8 cut(s) 380, 1037, 1043, 1130, 1250, 1409, 1455, 1658
GluI GCNGC 3 cut(s) 169, 681, 1572
GsaI CCCAGC 1 cut(s) 1930
HaeIII GGCC 1 cut(s) 2134
HapII CCGG 2 cut(s) 432, 1833
Hin1II CATG 5 cut(s) 13, 90, 1092, 1309, 1822
HindIII AAGCTT 3 cut(s) 504, 915, 1936
HinfI GANTC 9 cut(s) 107, 348, 364, 837, 964, 1323, 1468, 1615, 1822
HpaII CCGG 2 cut(s) 432, 1833
HphI GGTGA 5 cut(s) 178, 1046, 1295, 1529, 1598
Hpy166II GTNNAC 3 cut(s) 332, 1541, 1609
Hpy188I TCNGA 5 cut(s) 78, 669, 1000, 1113, 1427
Hpy188III TCNNGA 8 cut(s) 104, 371, 699, 1148, 1772, 1833, 1870, 1901
Hpy8I GTNNAC 3 cut(s) 332, 1541, 1609
Hpy99I CGWCG 1 cut(s) 2080
HpyAV CCTTC 6 cut(s) 173, 553, 622, 980, 1577, 1898
HpyCH4III ACNGT 2 cut(s) 1630, 1647
HpyF10VI GCNNNNNNNGC 3 cut(s) 1405, 2034, 2140
HpyF3I CTNAG 6 cut(s) 547, 697, 1019, 1395, 1513, 1742
Hsp92II CATG 5 cut(s) 13, 90, 1092, 1309, 1822
Kpn2I TCCGGA 1 cut(s) 1832
Kzo9I GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
LguI GCTCTTC 1 cut(s) 1787
LmnI GCTCC 5 cut(s) 855, 897, 1801, 1935, 1987
Lsp1109I GCAGC 3 cut(s) 155, 667, 1583
LweI GCATC 2 cut(s) 192, 2008
MaeI CTAG 8 cut(s) 380, 1037, 1043, 1130, 1250, 1409, 1455, 1658
MaeIII GTNAC 3 cut(s) 533, 1373, 2092
MalI GATC 6 cut(s) 102, 339, 1041, 1299, 1725, 2006
MboI GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
MflI RGATCY 2 cut(s) 1039, 1723
MhlI GDGCHC 4 cut(s) 240, 1005, 1806, 1984
MlyI GAGTC 3 cut(s) 101, 373, 973
MmeI TCCRAC 1 cut(s) 1527
MroI TCCGGA 1 cut(s) 1832
MroXI GAANNNNTTC 2 cut(s) 655, 994
MseI TTAA 6 cut(s) 126, 210, 303, 525, 1881, 2145
MslI CAYNNNNRTG 1 cut(s) 471
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 2 cut(s) 432, 1833
MspR9I CCNGG 1 cut(s) 1269
MvaI CCWGG 1 cut(s) 1269
MwoI GCNNNNNNNGC 3 cut(s) 1405, 2034, 2140
NcoI CCATGG 1 cut(s) 9
NdeII GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
NlaIII CATG 5 cut(s) 13, 90, 1092, 1309, 1822
NmeAIII GCCGAG 1 cut(s) 2063
NmuCI GTSAC 1 cut(s) 1373
NspI RCATGY 1 cut(s) 90
PaeI GCATGC 1 cut(s) 90
PciSI GCTCTTC 1 cut(s) 1787
PdmI GAANNNNTTC 2 cut(s) 655, 994
PfeI GAWTC 6 cut(s) 348, 837, 1323, 1468, 1615, 1822
PflMI CCANNNNNTGG 1 cut(s) 1735
PkrI GCNGC 3 cut(s) 170, 682, 1573
PleI GAGTC 3 cut(s) 101, 372, 972
PpsI GAGTC 3 cut(s) 101, 372, 972
PsiI TTATAA 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 1005
Psp6I CCWGG 1 cut(s) 1267
PspFI CCCAGC 1 cut(s) 1926
PspGI CCWGG 1 cut(s) 1267
PspPI GGNCC 3 cut(s) 117, 153, 2132
PstI CTGCAG 1 cut(s) 1573
PsuI RGATCY 2 cut(s) 1039, 1723
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 2 cut(s) 1624, 1649
RsaNI GTAC 2 cut(s) 1623, 1648
RseI CAYNNNNRTG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 1005
SapI GCTCTTC 1 cut(s) 1787
SaqAI TTAA 6 cut(s) 126, 210, 303, 525, 1881, 2145
SatI GCNGC 3 cut(s) 169, 681, 1572
Sau3AI GATC 6 cut(s) 100, 337, 1039, 1297, 1723, 2004
Sau96I GGNCC 3 cut(s) 117, 153, 2132
SchI GAGTC 3 cut(s) 101, 373, 973
ScrFI CCNGG 1 cut(s) 1269
SduI GDGCHC 4 cut(s) 240, 1005, 1806, 1984
SfaNI GCATC 2 cut(s) 192, 2008
SfcI CTRYAG 1 cut(s) 1569
SinI GGWCC 2 cut(s) 117, 153
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 1 cut(s) 1575
SmoI CTYRAG 1 cut(s) 1575
SphI GCATGC 1 cut(s) 90
SsiI CCGC 2 cut(s) 442, 1977
SspI AATATT 1 cut(s) 994
SspMI CTAG 8 cut(s) 380, 1037, 1043, 1130, 1250, 1409, 1455, 1658
SstI GAGCTC 1 cut(s) 1005
StyD4I CCNGG 1 cut(s) 1267
StyI CCWWGG 3 cut(s) 9, 1475, 1527
TaaI ACNGT 2 cut(s) 1630, 1647
TaqI TCGA 9 cut(s) 103, 205, 738, 819, 1336, 1401, 1491, 1559, 2007
TfiI GAWTC 6 cut(s) 348, 837, 1323, 1468, 1615, 1822
Tru1I TTAA 6 cut(s) 126, 210, 303, 525, 1881, 2145
Tru9I TTAA 6 cut(s) 126, 210, 303, 525, 1881, 2145
TscAI CASTG 3 cut(s) 339, 1066, 1372
TseFI GTSAC 1 cut(s) 1373
TseI GCWGC 3 cut(s) 168, 680, 1571
Tsp45I GTSAC 1 cut(s) 1373
TspGWI ACGGA 2 cut(s) 2017, 2064
TspRI CASTG 3 cut(s) 339, 1066, 1372
Van91I CCANNNNNTGG 1 cut(s) 1735
VpaK11BI GGWCC 2 cut(s) 117, 153
XapI RAATTY 5 cut(s) 511, 744, 760, 1253, 2070
XceI RCATGY 1 cut(s) 90
XmiI GTMKAC 2 cut(s) 1540, 1608
XmnI GAANNNNTTC 2 cut(s) 655, 994
XspI CTAG 8 cut(s) 380, 1037, 1043, 1130, 1250, 1409, 1455, 1658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.