Rorug05G0541600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005104
Physical Location & Seq
Forward (+)
73006562 .. 73007213
652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0541600.1

Sequence Viewer

Length: 576 bp
ATGGCGGGAAACACCATTAATGACGCTACCATGAAGGCCCTACAACTGAAGGTCGGAGGACTTCTCCGCTGCATCCACAAGGTGCAGAACACAAAGAGTCTTCTCAGGGATTCTCACTCCATCAAGATTTTGGAGCTCCGATCTGAGGCCTTGGAAGCTCGCACCAGAGAACGACTAGATCATTCTCGTAAAATCCTGACCGAACATTTCAAGGATCTCCTCCATTCTGTGCTCGATGCTTCTGGAATCTCCAATGTGGAGCATGAAATTCCACAATCCGATTTGGATTTTGAGACGCTCGAGTTGTTCGCATCACTGATCATGGTGGAGTGCGATCCAAACCTTGTCGCTGGTGCCTCATCGGTGATCGCGCCAGCCGCCGTCGCTCCAAACGCCGAGGCCGGGGCCGATTCGGTTGCGACCTGGTTCAAAGCAAGAATGGAGAAGCTTAACACAGTGGTTGATCTTGTTGTGTTGATCGATGGAATGAGTGCATTGATCTACTGCATCGAGCATTTCTCTGGAAAGGACATTGGTGCCTTCACTGATAAGCTGAGGAAGATTAAAAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.98

Weight (kDa)

6.16

Isoelectric Point (pI)

28.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 353, 536
AccII CGCG 1 cut(s) 371
AciI CCGC 3 cut(s) 5, 67, 378
AclWI GGATC 2 cut(s) 222, 329
AcsI RAATTY 1 cut(s) 267
AcuI CTGAAG 1 cut(s) 68
AdeI CACNNNGTG 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 145, 402
AgsI TTSAA 2 cut(s) 211, 430
AjnI CCWGG 1 cut(s) 422
AluBI AGCT 4 cut(s) 136, 158, 448, 553
AluI AGCT 4 cut(s) 136, 158, 448, 553
Alw21I GWGCWC 2 cut(s) 138, 234
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 2 cut(s) 222, 329
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 4 cut(s) 36, 147, 399, 405
ApeKI GCWGC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 267
AseI ATTAAT 1 cut(s) 18
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 2 cut(s) 37, 405
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 299
BanI GGYRCC 2 cut(s) 353, 536
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 92
Bbv12I GWGCWC 2 cut(s) 138, 234
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 128, 476
BceAI ACGGC 1 cut(s) 365
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 1 cut(s) 424
BclI TGATCA 1 cut(s) 318
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 1 cut(s) 176
BisI GCNGC 2 cut(s) 70, 378
BlsI GCNGC 2 cut(s) 71, 379
Bme1390I CCNGG 2 cut(s) 403, 424
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 37, 405
BmiI GGNNCC 3 cut(s) 355, 406, 538
BmrFI CCNGG 2 cut(s) 403, 424
BmsI GCATC 4 cut(s) 81, 226, 320, 516
BpiI GAAGAC 1 cut(s) 92
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 503, 535
Bpu10I CCTNAGC 1 cut(s) 554
BpuMI CCSGG 1 cut(s) 403
Bsa29I ATCGAT 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 150, 396, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 402
BseBI CCWGG 1 cut(s) 424
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 150, 396, 402
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 145, 402
BseMII CTCAG 3 cut(s) 118, 135, 545
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 56
BsgI GTGCAG 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 371
BshFI GGCC 4 cut(s) 38, 149, 401, 407
BshNI GGYRCC 2 cut(s) 353, 536
BshVI ATCGAT 1 cut(s) 480
BsiHKAI GWGCWC 2 cut(s) 138, 234
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 1 cut(s) 402
BslI CCNNNNNNNGG 2 cut(s) 145, 402
BsmAI GTCTC 1 cut(s) 287
BsmBI CGTCTC 1 cut(s) 287
BsnI GGCC 4 cut(s) 38, 149, 401, 407
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 138, 234
Bsp143I GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
BspACI CCGC 3 cut(s) 5, 67, 378
BspANI GGCC 4 cut(s) 38, 149, 401, 407
BspCNI CTCAG 3 cut(s) 117, 136, 546
BspDI ATCGAT 1 cut(s) 480
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 3 cut(s) 355, 406, 538
BspPI GGATC 2 cut(s) 222, 329
BspT107I GGYRCC 2 cut(s) 353, 536
BssECI CCNNGG 3 cut(s) 150, 396, 402
BssMI GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 160, 375
BstDEI CTNAG 3 cut(s) 104, 144, 554
BstF5I GGATG 1 cut(s) 72
BstFNI CGCG 1 cut(s) 371
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 9 cut(s) 143, 181, 217, 321, 337, 369, 466, 480, 501
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
BstMWI GCNNNNNNNGC 4 cut(s) 155, 377, 383, 392
BstNI CCWGG 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 401, 422
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 1 cut(s) 56
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 4 cut(s) 38, 149, 401, 407
BsuTUI ATCGAT 1 cut(s) 480
BtsCI GGATG 1 cut(s) 72
BtsIMutI CAGTG 3 cut(s) 314, 462, 543
Cac8I GCNNGC 2 cut(s) 160, 375
CfoI GCGC 1 cut(s) 373
Cfr13I GGNCC 2 cut(s) 37, 405
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 2 cut(s) 32, 304
CsiI ACCWGGT 1 cut(s) 422
CviAII CATG 3 cut(s) 31, 263, 322
CviJI RGCY 9 cut(s) 38, 136, 149, 158, 377, 401, 407, 448, 553
CviKI_1 RGCY 9 cut(s) 38, 136, 149, 158, 377, 401, 407, 448, 553
DdeI CTNAG 3 cut(s) 104, 144, 554
DpnI GATC 9 cut(s) 142, 180, 216, 320, 336, 368, 465, 479, 500
DpnII GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
DraIII CACNNNGTG 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 3 cut(s) 34, 266, 325
FaiI YATR 3 cut(s) 32, 264, 323
FatI CATG 3 cut(s) 30, 262, 321
FbaI TGATCA 1 cut(s) 318
Fnu4HI GCNGC 2 cut(s) 70, 378
FokI GGATG 1 cut(s) 59
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 70, 378
FspBI CTAG 1 cut(s) 176
GlaI GCGC 1 cut(s) 372
GluI GCNGC 2 cut(s) 70, 378
HaeIII GGCC 4 cut(s) 38, 149, 401, 407
HapII CCGG 1 cut(s) 402
HgaI GACGC 2 cut(s) 32, 304
HhaI GCGC 1 cut(s) 373
Hin1II CATG 3 cut(s) 34, 266, 325
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 4 cut(s) 97, 110, 246, 410
HpaII CCGG 1 cut(s) 402
HphI GGTGA 1 cut(s) 376
Hpy188I TCNGA 4 cut(s) 56, 140, 145, 280
Hpy188III TCNNGA 4 cut(s) 124, 196, 243, 522
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 3 cut(s) 28, 43, 550
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4V TGCA 4 cut(s) 72, 85, 494, 507
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 377, 383, 392
HpyF3I CTNAG 3 cut(s) 104, 144, 554
Hsp92II CATG 3 cut(s) 34, 266, 325
HspAI GCGC 1 cut(s) 371
Ksp22I TGATCA 1 cut(s) 318
Kzo9I GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
LmnI GCTCC 4 cut(s) 133, 141, 259, 391
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 4 cut(s) 81, 226, 320, 516
MabI ACCWGGT 1 cut(s) 422
MaeI CTAG 1 cut(s) 176
MalI GATC 9 cut(s) 142, 180, 216, 320, 336, 368, 465, 479, 500
MboI GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
MboII GAAGA 2 cut(s) 92, 571
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 138, 234
MluCI AATT 1 cut(s) 267
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 6 cut(s) 50, 139, 230, 367, 391, 549
MseI TTAA 3 cut(s) 18, 450, 564
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 2 cut(s) 403, 424
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 4 cut(s) 155, 377, 383, 392
NciI CCSGG 1 cut(s) 403
NdeII GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
NlaIII CATG 3 cut(s) 34, 266, 325
NlaIV GGNNCC 3 cut(s) 355, 406, 538
NmeAIII GCCGAG 1 cut(s) 421
PaeR7I CTCGAG 1 cut(s) 299
PceI AGGCCT 1 cut(s) 149
PfeI GAWTC 3 cut(s) 110, 246, 410
PkrI GCNGC 2 cut(s) 71, 379
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
PshBI ATTAAT 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 3 cut(s) 355, 406, 538
PspPI GGNCC 2 cut(s) 37, 405
PspXI VCTCGAGB 1 cut(s) 299
PsuI RGATCY 1 cut(s) 214
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 3 cut(s) 18, 450, 564
SatI GCNGC 2 cut(s) 70, 378
Sau3AI GATC 9 cut(s) 140, 178, 214, 318, 334, 366, 463, 477, 498
Sau96I GGNCC 2 cut(s) 37, 405
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 2 cut(s) 403, 424
SduI GDGCHC 2 cut(s) 138, 234
SetI ASST 8 cut(s) 54, 84, 138, 160, 345, 425, 450, 555
SexAI ACCWGGT 1 cut(s) 422
SfaNI GCATC 4 cut(s) 81, 226, 320, 516
Sfr274I CTCGAG 1 cut(s) 299
SlaI CTCGAG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 1 cut(s) 267
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 3 cut(s) 5, 67, 378
SspMI CTAG 1 cut(s) 176
SstI GAGCTC 1 cut(s) 138
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 401, 422
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 457
TaqI TCGA 4 cut(s) 234, 300, 480, 510
TaqII GACCGA 1 cut(s) 215
TasI AATT 1 cut(s) 267
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 3 cut(s) 110, 246, 410
Tru1I TTAA 3 cut(s) 18, 450, 564
Tru9I TTAA 3 cut(s) 18, 450, 564
TscAI CASTG 3 cut(s) 321, 462, 550
TseI GCWGC 1 cut(s) 69
TspDTI ATGAA 2 cut(s) 47, 279
TspRI CASTG 3 cut(s) 321, 462, 550
VspI ATTAAT 1 cut(s) 18
XapI RAATTY 1 cut(s) 267
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 299
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.