Rorug05G0542200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005104
Physical Location & Seq
Reverse (-)
73043558 .. 73050232
6675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0542200.1

Sequence Viewer

Length: 2334 bp
ATGGCGCTGAGGAGAAAATTGGGGGAGAGTTCGAATTTCAGTTGCAAAATGATGTTGAGCTTTTCGAGGTATTACAGCTCAAACAAAGTGGACTTAAAGAAGCTGAGGCCTATGATTCTGAAGAGAATCCAGGACCGAGCTAAGGACTATCCGGTGCAGAGCATGATACCAGTGGCCGAGGAGGTCCTTAATTCCCGGAGGCTTCTTATTCAAGGCGTCTCTACCCTCCTCAAAGTGATCCCAGTTGTGGCCTGCAAATTCTGTCCAGAAGTACACATTAGCGATAAAGGTCACTTAATTCAGACTTGTTGTGGTTTCAAACGTCGTGGCAGAAATCGGGTTCATGAGTGGATAACTGGTAGTTTGAAAGATGTACTTGTTCCAGTAGAGGCATTTCACTTGAAGCACATGTTCCAGGGTGTTATCAAACATAACCAAAGGTTTGATTATGACCGTGTTCCTGCAGTTGTTGAGCTGTGCTGGCAAGCAGGGGCAAATGATGAAAATCTGTATTCTGGTACTTGGAACCCAGAAAGTGATTGTGTTGCTGTTGAAGGAGCTGAGTCATTGTCACCTGCTGAACTCACTCTGGTTGCCAATGGAACTTTGAGGGCCTGGGAAATACTTAGAAATGGCGTGAAGAAATTGTTGATGGTTTATCCAGCAAAAGTGTGTAAAGACTGTTCAGAAGTCCATGTTGGGCCATCTGGGCATAAGGCGAGGCTTTGTGGACTTTTTAAGTATGAGCGTTGGCAAGGAACCCACTTTTGGAGAAAAGCATCTGTAAATGATCTTGTTCCTCCAAACATTGTATGGCGGAGGCGGCGTCAAGATCCTCTTGTTCTTTTGAATGAAGGAAGGGGCTATTACGGGCATGCTCCAGCAGTAGTAGAACTATGCGCACAGGCAGGTGCTATTGTGCCTTCAAAGTATCGTTGTATGATGAAAGTGGAGGGTTTAACTTCAGTTGTAAATCAAGGAAAGTTGCTCCAGAAAACAATGAGCCGAGAGAAAGGAAGGCAGGCTAGACATTGGAAATTAAATTGTGGCATTGTTGATGAAATGGAAGATGTTCATGATGATGTGGGTGGCGAACTTCGTGGTAGAGAATTGGCTAGCTTGGAGTCTGATGCTCATGAATCGGACTATGTGAATAGTGAAGTAGATGATACCACTGATGCTCAAGCAAGTAGAGCAGAAGACACCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAGAAATACCGGCAAACTAGGTTAGAACAATACAAGAATTATGAAAACATCCCTAATTCCGGAGAAGGGACAGGGAAGGACTGCAACATTACAGAAAAAGGATCTTTATATTATGAGTTTCAATGCAACACAAGATCAGTGAAACCAACTATCCTTCATTTTCAGTTGCGTAACTTGGTCTGGGCTACATCAAAGCATGATGTCTACCTTATGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCGAAGCATCTGAGGTTCTAAATGTTTCAGGACATGTCACACCATCAGAGAAACATCCTGGAAGTCTCTTGGAAGGATTTACACAGACTCAAGTTAGTAGTCTTGCAGTGAAAGATAAGTTGCTAGTTGCTGGAGGATTCCAAGGAGAACTTATTTGCAAGCATCTTGATCAACCTGGAGTTAGCTTTTGTTCGAGGACAACTGATGATCATAATGCCATTACAAATGCTATTGAGATTTATGACAATCCCAGTGGTGCAGCTCACTTTACAGCCTCAAGTAATGATTGTGGAGTCAGAGACTTTGACATGGAGACATTTCAGCTTTCTAAGCATTTCCGTTTTCCTTGGCCAGCGAATCATACTTCTCTGAGTCCCGATGGTAAACTTTTTGTGATAGTTGGTGACAATCCAGATGGGATGTTGGTCGACTCTCAAACTGGCAAGACGGTGACGCCTTTATGTGGACACTTGGATTTCTCGTTTGCATCAGCGTGGCATCCTGATGGTGTCACCTTTGCTACTGGGAACCAGGACAAAACCTGTCGGGTTTGGGATGTTCGAAACCTATCCAAGTCAGTTGCTGTTTTAAAGGGAAACCTTGGAGCTATTCGGTCAATCCGTTATACTTCTGATGGTCAGTATATGATGATGGCAGAGCCATCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTACGAGAAGGAGCAGGAAATCGATTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCAGACACAGAGTCGCTTTTCATTGGAGTATGGGATCGTACATATGGTGGTCTTCTCGAGTATGGAAGACAAAAGTGTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

777

Amino Acids

87.29

Weight (kDa)

6.96

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 29 - 166 4.8e-42 APO RNA-binding
APO_RNA-bind PF05634 194 - 308 2.9e-19 APO RNA-binding
WD40_Prp19 PF24814 529 - 729 2.9e-14 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 585 - 732 7.8e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 586 - 776 2.5e-14 WDR5 beta-propeller domain
WD40_Gbeta PF25391 590 - 726 2.9e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 620 - 730 6.3e-07 WDR55
WD40_CDC20-Fz PF24807 622 - 755 8.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 639 - 762 1.6e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 646 - 761 5.8e-14 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 662 - 761 4e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 583, 899
Acc16I TGCGCA 1 cut(s) 901
Acc36I ACCTGC 2 cut(s) 583, 899
AccI GTMKAC 2 cut(s) 1446, 1920
AccIII TCCGGA 1 cut(s) 1301
AciI CCGC 2 cut(s) 817, 823
AclWI GGATC 4 cut(s) 232, 827, 1351, 2289
AcoI YGGCCR 2 cut(s) 174, 1841
AcsI RAATTY 2 cut(s) 34, 257
AcuI CTGAAG 2 cut(s) 140, 948
AcyI GRCGYC 3 cut(s) 216, 826, 1946
AfaI GTAC 5 cut(s) 273, 375, 520, 2192, 2287
AfiI CCNNNNNNNGG 6 cut(s) 142, 247, 768, 1301, 1308, 1955
AflIII ACRYGT 2 cut(s) 408, 1525
AgsI TTSAA 8 cut(s) 212, 319, 367, 403, 554, 850, 927, 1364
AhdI GACNNNNNGTC 1 cut(s) 2257
AjnI CCWGG 6 cut(s) 129, 414, 614, 1549, 1666, 2022
AjuI GAANNNNNNNTTGG 4 cut(s) 681, 713, 751, 783
Alw26I GTCTC 4 cut(s) 223, 1562, 1785, 1799
AlwI GGATC 4 cut(s) 232, 827, 1351, 2289
AlwNI CAGNNNCTG 1 cut(s) 2075
Ama87I CYCGRG 1 cut(s) 2303
Aor13HI TCCGGA 1 cut(s) 1301
AoxI GGCC 6 cut(s) 107, 174, 249, 612, 701, 1841
ApeKI GCWGC 1 cut(s) 1751
ApoI RAATTY 2 cut(s) 34, 257
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 2 cut(s) 7, 902
AspS9I GGNCC 4 cut(s) 133, 184, 612, 701
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 5 cut(s) 564, 1907, 1954, 1996, 2234
AsuII TTCGAA 2 cut(s) 32, 2053
AsuNHI GCTAGC 1 cut(s) 1115
AvaI CYCGRG 1 cut(s) 2303
AvaII GGWCC 2 cut(s) 133, 184
BalI TGGCCA 1 cut(s) 1843
BbsI GAAGAC 4 cut(s) 1206, 1473, 2291, 2320
BbvCI CCTCAGC 2 cut(s) 8, 104
BbvI GCAGC 1 cut(s) 1763
BccI CCATC 9 cut(s) 646, 712, 1543, 1865, 1901, 1991, 2120, 2137, 2161
BciT130I CCWGG 6 cut(s) 131, 416, 616, 1551, 1668, 2024
BclI TGATCA 2 cut(s) 1660, 1699
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 4 cut(s) 223, 1562, 1785, 1799
BfaI CTAG 5 cut(s) 1026, 1116, 1239, 1260, 1616
BfmI CTRYAG 1 cut(s) 462
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 2 cut(s) 583, 899
BglI GCCNNNNNGGC 1 cut(s) 709
BisI GCNGC 2 cut(s) 824, 1752
BlsI GCNGC 2 cut(s) 825, 1753
Bme1390I CCNGG 7 cut(s) 131, 196, 416, 616, 1551, 1668, 2024
Bme18I GGWCC 2 cut(s) 133, 184
BmeRI GACNNNNNGTC 1 cut(s) 2257
BmeT110I CYCGRG 1 cut(s) 2303
BmgT120I GGNCC 4 cut(s) 133, 184, 612, 701
BmiI GGNNCC 3 cut(s) 527, 760, 2021
BmrFI CCNGG 7 cut(s) 131, 196, 416, 616, 1551, 1668, 2024
BmrI ACTGGG 3 cut(s) 236, 1737, 2025
BmsI GCATC 7 cut(s) 788, 1120, 1168, 1508, 1663, 1988, 1999
BmtI GCTAGC 1 cut(s) 1119
BmuI ACTGGG 3 cut(s) 236, 1737, 2025
BpiI GAAGAC 4 cut(s) 1206, 1473, 2291, 2320
BpmI CTGGAG 4 cut(s) 864, 974, 1644, 1689
Bpu10I CCTNAGC 3 cut(s) 8, 104, 141
Bpu14I TTCGAA 2 cut(s) 32, 2053
BpuEI CTTGAG 3 cut(s) 1167, 1566, 1753
BpuMI CCSGG 1 cut(s) 196
Bsa29I ATCGAT 1 cut(s) 2211
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 3 cut(s) 216, 826, 1946
BsaJI CCNNGG 8 cut(s) 177, 415, 615, 1207, 1217, 1633, 1838, 2092
BsaWI WCCGGW 2 cut(s) 151, 1301
BsaXI ACNNNNNCTCC 2 cut(s) 811, 841
Bsc4I CCNNNNNNNGG 6 cut(s) 142, 247, 768, 1301, 1308, 1955
Bse118I RCCGGY 1 cut(s) 1251
Bse1I ACTGG 7 cut(s) 170, 242, 361, 383, 1743, 1936, 2020
Bse8I GATNNNNATC 1 cut(s) 504
BseAI TCCGGA 1 cut(s) 1301
BseBI CCWGG 6 cut(s) 131, 416, 616, 1551, 1668, 2024
BseCI ATCGAT 1 cut(s) 2211
BseDI CCNNGG 8 cut(s) 177, 415, 615, 1207, 1217, 1633, 1838, 2092
BseGI GGATG 5 cut(s) 1290, 1546, 1917, 1990, 2053
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 6 cut(s) 142, 247, 768, 1301, 1308, 1955
BseMII CTCAG 4 cut(s) 95, 552, 1494, 1853
BseNI ACTGG 7 cut(s) 170, 242, 361, 383, 1743, 1936, 2020
BseRI GAGGAG 3 cut(s) 25, 194, 218
BseXI GCAGC 1 cut(s) 1763
BsgI GTGCAG 2 cut(s) 176, 1770
BshFI GGCC 6 cut(s) 109, 176, 251, 614, 703, 1843
BshVI ATCGAT 1 cut(s) 2211
BsiHKCI CYCGRG 1 cut(s) 2303
BsiSI CCGG 4 cut(s) 152, 196, 1252, 1302
BslFI GGGAC 2 cut(s) 1324, 1851
BslI CCNNNNNNNGG 6 cut(s) 142, 247, 768, 1301, 1308, 1955
BsmAI GTCTC 4 cut(s) 223, 1562, 1785, 1799
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 2 cut(s) 1324, 1851
BsnI GGCC 6 cut(s) 109, 176, 251, 614, 703, 1843
BsoBI CYCGRG 1 cut(s) 2303
Bsp119I TTCGAA 2 cut(s) 32, 2053
Bsp13I TCCGGA 1 cut(s) 1301
Bsp143I GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
BspACI CCGC 2 cut(s) 817, 823
BspANI GGCC 6 cut(s) 109, 176, 251, 614, 703, 1843
BspCNI CTCAG 4 cut(s) 96, 553, 1495, 1854
BspDI ATCGAT 1 cut(s) 2211
BspEI TCCGGA 1 cut(s) 1301
BspHI TCATGA 3 cut(s) 343, 1075, 1135
BspLI GGNNCC 3 cut(s) 527, 760, 2021
BspMAI CTGCAG 1 cut(s) 466
BspMI ACCTGC 2 cut(s) 583, 899
BspOI GCTAGC 1 cut(s) 1119
BspPI GGATC 4 cut(s) 232, 827, 1351, 2289
BspT104I TTCGAA 2 cut(s) 32, 2053
BsrFI RCCGGY 1 cut(s) 1251
BsrI ACTGG 7 cut(s) 170, 242, 361, 383, 1743, 1936, 2020
BssAI RCCGGY 1 cut(s) 1251
BssECI CCNNGG 8 cut(s) 177, 415, 615, 1207, 1217, 1633, 1838, 2092
BssMI GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
BssNI GRCGYC 3 cut(s) 216, 826, 1946
BssT1I CCWWGG 5 cut(s) 1207, 1217, 1633, 1838, 2092
Bst2UI CCWGG 6 cut(s) 131, 416, 616, 1551, 1668, 2024
Bst4CI ACNGT 3 cut(s) 455, 683, 1942
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 3 cut(s) 216, 826, 1946
BstBI TTCGAA 2 cut(s) 32, 2053
BstC8I GCNNGC 8 cut(s) 253, 482, 486, 876, 1023, 1117, 1652, 1845
BstDEI CTNAG 9 cut(s) 8, 104, 141, 561, 626, 1229, 1503, 1821, 1862
BstF5I GGATG 5 cut(s) 1290, 1546, 1917, 1990, 2053
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 902
BstKTI GATC 8 cut(s) 240, 793, 835, 1346, 1379, 1663, 1702, 2284
BstMAI GTCTC 4 cut(s) 223, 1562, 1785, 1799
BstMBI GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
BstMWI GCNNNNNNNGC 5 cut(s) 481, 709, 823, 1193, 1822
BstNI CCWGG 6 cut(s) 131, 416, 616, 1551, 1668, 2024
BstNSI RCATGY 4 cut(s) 412, 878, 1529, 2171
BstSCI CCNGG 7 cut(s) 129, 194, 414, 614, 1549, 1666, 2022
BstSFI CTRYAG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 1763
BstV2I GAAGAC 4 cut(s) 1206, 1473, 2291, 2320
BstX2I RGATCY 2 cut(s) 832, 1343
BstYI RGATCY 2 cut(s) 832, 1343
Bsu15I ATCGAT 1 cut(s) 2211
BsuRI GGCC 6 cut(s) 109, 176, 251, 614, 703, 1843
BsuTUI ATCGAT 1 cut(s) 2211
BtsCI GGATG 5 cut(s) 1290, 1546, 1917, 1990, 2053
BtsI GCAGTG 1 cut(s) 1605
BtsIMutI CAGTG 5 cut(s) 177, 1173, 1386, 1605, 1750
BveI ACCTGC 2 cut(s) 583, 899
Cac8I GCNNGC 8 cut(s) 253, 482, 486, 876, 1023, 1117, 1652, 1845
CaiI CAGNNNCTG 1 cut(s) 2075
CciI TCATGA 3 cut(s) 343, 1075, 1135
CfoI GCGC 2 cut(s) 7, 902
Cfr10I RCCGGY 1 cut(s) 1251
Cfr13I GGNCC 4 cut(s) 133, 184, 612, 701
ClaI ATCGAT 1 cut(s) 2211
CseI GACGC 3 cut(s) 205, 815, 1954
Csp6I GTAC 5 cut(s) 272, 374, 519, 2191, 2286
CspCI CAANNNNNGTGG 6 cut(s) 69, 104, 307, 342, 1726, 1761
CviQI GTAC 5 cut(s) 272, 374, 519, 2191, 2286
DdeI CTNAG 9 cut(s) 8, 104, 141, 561, 626, 1229, 1503, 1821, 1862
DpnI GATC 8 cut(s) 239, 792, 834, 1345, 1378, 1662, 1701, 2283
DpnII GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
DraI TTTAAA 1 cut(s) 2082
DriI GACNNNNNGTC 1 cut(s) 2257
EaeI YGGCCR 2 cut(s) 174, 1841
Eam1104I CTCTTC 1 cut(s) 116
Eam1105I GACNNNNNGTC 1 cut(s) 2257
EarI CTCTTC 1 cut(s) 116
EciI GGCGGA 1 cut(s) 832
Eco130I CCWWGG 5 cut(s) 1207, 1217, 1633, 1838, 2092
Eco147I AGGCCT 1 cut(s) 109
Eco32I GATATC 1 cut(s) 2229
Eco47I GGWCC 2 cut(s) 133, 184
Eco57I CTGAAG 2 cut(s) 140, 948
Eco88I CYCGRG 1 cut(s) 2303
EcoO109I RGGNCCY 2 cut(s) 184, 612
EcoRII CCWGG 6 cut(s) 129, 414, 614, 1549, 1666, 2022
EcoRV GATATC 1 cut(s) 2229
EcoT14I CCWWGG 5 cut(s) 1207, 1217, 1633, 1838, 2092
ErhI CCWWGG 5 cut(s) 1207, 1217, 1633, 1838, 2092
Esp3I CGTCTC 1 cut(s) 223
FalI AAGNNNNNCTT 6 cut(s) 360, 392, 822, 854, 1626, 1658
FaqI GGGAC 2 cut(s) 1324, 1851
FauNDI CATATG 1 cut(s) 2290
FbaI TGATCA 2 cut(s) 1660, 1699
FblI GTMKAC 2 cut(s) 1446, 1920
Fnu4HI GCNGC 2 cut(s) 824, 1752
FokI GGATG 5 cut(s) 1277, 1533, 1924, 1977, 2060
Fsp4HI GCNGC 2 cut(s) 824, 1752
FspAI RTGCGCAY 1 cut(s) 901
FspBI CTAG 5 cut(s) 1026, 1116, 1239, 1260, 1616
FspI TGCGCA 1 cut(s) 901
GlaI GCGC 2 cut(s) 6, 901
GluI GCNGC 2 cut(s) 824, 1752
GsuI CTGGAG 4 cut(s) 864, 974, 1644, 1689
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 109, 176, 251, 614, 703, 1843
HapII CCGG 4 cut(s) 152, 196, 1252, 1302
HgaI GACGC 3 cut(s) 205, 815, 1954
HhaI GCGC 2 cut(s) 7, 902
Hin1I GRCGYC 3 cut(s) 216, 826, 1946
Hin6I GCGC 2 cut(s) 5, 900
HinP1I GCGC 2 cut(s) 5, 900
HincII GTYRAC 1 cut(s) 1921
HindII GTYRAC 1 cut(s) 1921
HpaII CCGG 4 cut(s) 152, 196, 1252, 1302
HphI GGTGA 5 cut(s) 564, 1907, 1954, 1996, 2234
Hpy166II GTNNAC 7 cut(s) 91, 274, 731, 1447, 1877, 1921, 1958
Hpy8I GTNNAC 7 cut(s) 91, 274, 731, 1447, 1877, 1921, 1958
Hpy99I CGWCG 1 cut(s) 327
HpyCH4III ACNGT 3 cut(s) 455, 683, 1942
HpyCH4IV ACGT 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 5 cut(s) 481, 709, 823, 1193, 1822
HpyF3I CTNAG 9 cut(s) 8, 104, 141, 561, 626, 1229, 1503, 1821, 1862
HpySE526I ACGT 1 cut(s) 322
Hsp92I GRCGYC 3 cut(s) 216, 826, 1946
HspAI GCGC 2 cut(s) 5, 900
Kpn2I TCCGGA 1 cut(s) 1301
Ksp22I TGATCA 2 cut(s) 1660, 1699
Kzo9I GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
LmnI GCTCC 5 cut(s) 557, 883, 993, 2096, 2200
Lsp1109I GCAGC 1 cut(s) 1763
LweI GCATC 7 cut(s) 788, 1120, 1168, 1508, 1663, 1988, 1999
MaeI CTAG 5 cut(s) 1026, 1116, 1239, 1260, 1616
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 8 cut(s) 290, 570, 1412, 1455, 1528, 1895, 1942, 2002
MalI GATC 8 cut(s) 239, 792, 834, 1345, 1378, 1662, 1701, 2283
MboI GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
MboII GAAGA 7 cut(s) 133, 652, 1079, 1211, 1473, 2291, 2325
MflI RGATCY 2 cut(s) 832, 1343
MlsI TGGCCA 1 cut(s) 1843
MluNI TGGCCA 1 cut(s) 1843
MlyI GAGTC 7 cut(s) 572, 1133, 1573, 1794, 1873, 1916, 2267
Mox20I TGGCCA 1 cut(s) 1843
MroI TCCGGA 1 cut(s) 1301
MroXI GAANNNNTTC 1 cut(s) 1071
MscI TGGCCA 1 cut(s) 1843
MseI TTAA 9 cut(s) 95, 189, 296, 738, 959, 1040, 1487, 2081, 2332
MslI CAYNNNNRTG 4 cut(s) 1080, 1984, 1995, 2172
Msp20I TGGCCA 1 cut(s) 1843
MspI CCGG 4 cut(s) 152, 196, 1252, 1302
MspR9I CCNGG 7 cut(s) 131, 196, 416, 616, 1551, 1668, 2024
MvaI CCWGG 6 cut(s) 131, 416, 616, 1551, 1668, 2024
MwoI GCNNNNNNNGC 5 cut(s) 481, 709, 823, 1193, 1822
NciI CCSGG 1 cut(s) 196
NdeI CATATG 1 cut(s) 2290
NdeII GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
NheI GCTAGC 1 cut(s) 1115
NlaIV GGNNCC 3 cut(s) 527, 760, 2021
NmeAIII GCCGAG 2 cut(s) 202, 1031
NmuCI GTSAC 7 cut(s) 290, 570, 1455, 1528, 1895, 1942, 2002
NsbI TGCGCA 1 cut(s) 901
NspI RCATGY 4 cut(s) 412, 878, 1529, 2171
NspV TTCGAA 2 cut(s) 32, 2053
PaeI GCATGC 1 cut(s) 878
PaeR7I CTCGAG 1 cut(s) 2303
PagI TCATGA 3 cut(s) 343, 1075, 1135
PaqCI CACCTGC 2 cut(s) 583, 899
PceI AGGCCT 1 cut(s) 109
PciI ACATGT 2 cut(s) 408, 1525
PcsI WCGNNNNNNNCGW 1 cut(s) 2110
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 5 cut(s) 115, 126, 1139, 1629, 1849
PfoI TCCNGGA 3 cut(s) 129, 194, 1549
PkrI GCNGC 2 cut(s) 825, 1753
PleI GAGTC 7 cut(s) 571, 1132, 1573, 1793, 1872, 1916, 2266
PpsI GAGTC 7 cut(s) 571, 1132, 1573, 1793, 1872, 1916, 2266
PpuMI RGGWCCY 1 cut(s) 184
PscI ACATGT 2 cut(s) 408, 1525
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 6 cut(s) 129, 414, 614, 1549, 1666, 2022
PspGI CCWGG 6 cut(s) 129, 414, 614, 1549, 1666, 2022
PspN4I GGNNCC 3 cut(s) 527, 760, 2021
PspPI GGNCC 4 cut(s) 133, 184, 612, 701
PspPPI RGGWCCY 1 cut(s) 184
PsrI GAACNNNNNNTAC 2 cut(s) 667, 699
PstI CTGCAG 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 2075
PsuI RGATCY 2 cut(s) 832, 1343
RsaI GTAC 5 cut(s) 273, 375, 520, 2192, 2287
RsaNI GTAC 5 cut(s) 272, 374, 519, 2191, 2286
RseI CAYNNNNRTG 4 cut(s) 1080, 1984, 1995, 2172
SalI GTCGAC 1 cut(s) 1919
SaqAI TTAA 9 cut(s) 95, 189, 296, 738, 959, 1040, 1487, 2081, 2332
SatI GCNGC 2 cut(s) 824, 1752
Sau3AI GATC 8 cut(s) 237, 790, 832, 1343, 1376, 1660, 1699, 2281
Sau96I GGNCC 4 cut(s) 133, 184, 612, 701
SchI GAGTC 7 cut(s) 572, 1133, 1573, 1794, 1873, 1916, 2267
ScrFI CCNGG 7 cut(s) 131, 196, 416, 616, 1551, 1668, 2024
SfaNI GCATC 7 cut(s) 788, 1120, 1168, 1508, 1663, 1988, 1999
SfcI CTRYAG 1 cut(s) 462
Sfr274I CTCGAG 1 cut(s) 2303
SfuI TTCGAA 2 cut(s) 32, 2053
SinI GGWCC 2 cut(s) 133, 184
SlaI CTCGAG 1 cut(s) 2303
SmiMI CAYNNNNRTG 4 cut(s) 1080, 1984, 1995, 2172
SmlI CTYRAG 4 cut(s) 1182, 1581, 1768, 2303
SmoI CTYRAG 4 cut(s) 1182, 1581, 1768, 2303
SphI GCATGC 1 cut(s) 878
SseBI AGGCCT 1 cut(s) 109
SsiI CCGC 2 cut(s) 817, 823
SspMI CTAG 5 cut(s) 1026, 1116, 1239, 1260, 1616
StuI AGGCCT 1 cut(s) 109
StyD4I CCNGG 7 cut(s) 129, 194, 414, 614, 1549, 1666, 2022
StyI CCWWGG 5 cut(s) 1207, 1217, 1633, 1838, 2092
TaaI ACNGT 3 cut(s) 455, 683, 1942
TaiI ACGT 1 cut(s) 325
TaqI TCGA 7 cut(s) 32, 65, 1685, 1920, 2053, 2211, 2304
TaqII GACCGA 2 cut(s) 150, 2094
TatI WGTACW 2 cut(s) 271, 373
TauI GCSGC 1 cut(s) 826
TfiI GAWTC 5 cut(s) 115, 126, 1139, 1629, 1849
Tru1I TTAA 9 cut(s) 95, 189, 296, 738, 959, 1040, 1487, 2081, 2332
Tru9I TTAA 9 cut(s) 95, 189, 296, 738, 959, 1040, 1487, 2081, 2332
TscAI CASTG 5 cut(s) 177, 1180, 1386, 1605, 1750
TseFI GTSAC 7 cut(s) 290, 570, 1455, 1528, 1895, 1942, 2002
TseI GCWGC 1 cut(s) 1751
Tsp45I GTSAC 7 cut(s) 290, 570, 1455, 1528, 1895, 1942, 2002
TspGWI ACGGA 2 cut(s) 1820, 2102
TspRI CASTG 5 cut(s) 177, 1180, 1386, 1605, 1750
VpaK11BI GGWCC 2 cut(s) 133, 184
XapI RAATTY 2 cut(s) 34, 257
XceI RCATGY 4 cut(s) 412, 878, 1529, 2171
XcmI CCANNNNNNNNNTGG 1 cut(s) 810
XhoI CTCGAG 1 cut(s) 2303
XmiI GTMKAC 2 cut(s) 1446, 1920
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 5 cut(s) 1026, 1116, 1239, 1260, 1616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.