Rorug05G0555500
ERF Family

Belongs to the AB hydrolase superfamily. Lipase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005091
Physical Location & Seq
Forward (+)
74366534 .. 74367409
876 bp
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UTR
Exon/CDS
Intron
Rorug05G0555500.1

Sequence Viewer

Length: 579 bp
ATGTCGATCCCGCCGAATTCACTATGGAGAGGATGTGCAGCAAACTTCCCTCAACCTGTCGATGAAGACGACGTGCGTGCAGCTCAATTTTTGGTGGAAACTGTTTATTACTTTAATTGCCATATTGCAGATCCCCTTCTGGCTGACATCGAGTACAGAAGCAGCAGTGGAGAAGGAGACATACTCCACCATGTGTTCCGCTGGGATCTTACTCCCTGTCATGAGGTCTTTCAACATGGCTTCCAAGCAAGGCGTCAAGATAACACTCCCGACGAGGTCTACTACAATCTGGCTCACTACATCCGTCAGGGAGGTAGGCCTCTTGATTCCCGACGACCCACCACCCACGCCTTCATTAGCACCACGCTCGACAGTGCATGGCATCCCACCGTCACTGAGACTGTGAGTGAAGTATGTCGTTACGAGATATACGTTCCCGGTGGCATTTGGGTTGCTCAGACTCTTGGTGATCATAATGCCAAAGACCCAGACGTCGACGAGGTGTGCTTTGTTGCTGGCATTGCCCCTCAATACATTCGGTCTGCCAACGCTTCAAGCTCACTTCTGGAGAGTCGGTAA

Protein Analysis

192

Amino Acids

21.7

Weight (kDa)

5.01

Isoelectric Point (pI)

57.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Scabin-like PF22596 66 - 184 3.1e-21 Scabin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AatII GACGTC 1 cut(s) 495
AccI GTMKAC 2 cut(s) 279, 495
AciI CCGC 2 cut(s) 11, 199
AclWI GGATC 2 cut(s) 125, 213
AcsI RAATTY 1 cut(s) 16
AcyI GRCGYC 2 cut(s) 253, 492
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 1 cut(s) 155
AgsI TTSAA 2 cut(s) 233, 555
AjiI CACGTC 1 cut(s) 73
AluBI AGCT 2 cut(s) 83, 558
AluI AGCT 2 cut(s) 83, 558
Alw26I GTCTC 2 cut(s) 171, 392
AlwI GGATC 2 cut(s) 125, 213
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 3 cut(s) 38, 80, 162
ApoI RAATTY 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 438
AsuHPI GGTGA 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 165, 197
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 3 cut(s) 50, 92, 174
BcgI CGANNNNNNTGC 4 cut(s) 59, 93, 349, 383
BclI TGATCA 1 cut(s) 469
BcnI CCSGG 1 cut(s) 438
BcoDI GTCTC 2 cut(s) 171, 392
BisI GCNGC 3 cut(s) 39, 81, 163
BlsI GCNGC 3 cut(s) 40, 82, 164
Bme1390I CCNGG 1 cut(s) 438
BmgBI CACGTC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 1 cut(s) 391
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpuMI CCSGG 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 253, 492
BsaXI ACNNNNNCTCC 3 cut(s) 19, 49, 560
Bse3DI GCAATG 1 cut(s) 519
BseGI GGATG 3 cut(s) 38, 300, 382
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 387, 470
BseXI GCAGC 3 cut(s) 50, 92, 174
BseYI CCCAGC 1 cut(s) 201
BsgI GTGCAG 2 cut(s) 57, 99
BshFI GGCC 1 cut(s) 319
BsiSI CCGG 1 cut(s) 438
BsmAI GTCTC 2 cut(s) 171, 392
BsnI GGCC 1 cut(s) 319
Bsp143I GATC 4 cut(s) 6, 130, 205, 469
BspACI CCGC 2 cut(s) 11, 199
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 2 cut(s) 388, 469
BspHI TCATGA 1 cut(s) 220
BspPI GGATC 2 cut(s) 125, 213
BsrDI GCAATG 1 cut(s) 519
BssMI GATC 4 cut(s) 6, 130, 205, 469
BssNI GRCGYC 2 cut(s) 253, 492
Bst4CI ACNGT 4 cut(s) 103, 374, 391, 403
BstACI GRCGYC 2 cut(s) 253, 492
BstC8I GCNNGC 2 cut(s) 78, 517
BstDEI CTNAG 2 cut(s) 396, 456
BstF5I GGATG 3 cut(s) 38, 300, 382
BstKTI GATC 4 cut(s) 9, 133, 208, 472
BstMAI GTCTC 2 cut(s) 171, 392
BstMBI GATC 4 cut(s) 6, 130, 205, 469
BstMWI GCNNNNNNNGC 1 cut(s) 521
BstSCI CCNGG 1 cut(s) 436
BstV1I GCAGC 3 cut(s) 50, 92, 174
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 2 cut(s) 130, 205
BstYI RGATCY 2 cut(s) 130, 205
BsuRI GGCC 1 cut(s) 319
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 3 cut(s) 38, 300, 382
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 3 cut(s) 172, 379, 393
Cac8I GCNNGC 2 cut(s) 78, 517
CciI TCATGA 1 cut(s) 220
CseI GACGC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 154
CviAII CATG 4 cut(s) 191, 221, 236, 378
CviJI RGCY 6 cut(s) 83, 143, 240, 293, 319, 558
CviKI_1 RGCY 6 cut(s) 83, 143, 240, 293, 319, 558
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 2 cut(s) 396, 456
DpnI GATC 4 cut(s) 8, 132, 207, 471
DpnII GATC 4 cut(s) 6, 130, 205, 469
DraIII CACNNNGTG 1 cut(s) 193
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eco147I AGGCCT 1 cut(s) 319
EcoRI GAATTC 1 cut(s) 16
FaeI CATG 4 cut(s) 194, 224, 239, 381
FatI CATG 4 cut(s) 190, 220, 235, 377
FauI CCCGC 1 cut(s) 18
FbaI TGATCA 1 cut(s) 469
FblI GTMKAC 2 cut(s) 279, 495
Fnu4HI GCNGC 3 cut(s) 39, 81, 163
FokI GGATG 3 cut(s) 45, 287, 369
Fsp4HI GCNGC 3 cut(s) 39, 81, 163
GluI GCNGC 3 cut(s) 39, 81, 163
GsaI CCCAGC 1 cut(s) 205
HaeIII GGCC 1 cut(s) 319
HapII CCGG 1 cut(s) 438
HgaI GACGC 1 cut(s) 242
Hin1I GRCGYC 2 cut(s) 253, 492
Hin1II CATG 4 cut(s) 194, 224, 239, 381
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 3 cut(s) 326, 460, 571
HpaII CCGG 1 cut(s) 438
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 2 cut(s) 280, 496
Hpy188I TCNGA 1 cut(s) 459
Hpy188III TCNNGA 6 cut(s) 221, 257, 269, 323, 330, 566
Hpy8I GTNNAC 2 cut(s) 280, 496
Hpy99I CGWCG 5 cut(s) 74, 275, 336, 497, 500
HpyAV CCTTC 3 cut(s) 146, 167, 361
HpyCH4III ACNGT 4 cut(s) 103, 374, 391, 403
HpyCH4IV ACGT 3 cut(s) 72, 432, 492
HpyCH4V TGCA 4 cut(s) 38, 80, 128, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 521
HpyF3I CTNAG 2 cut(s) 396, 456
HpySE526I ACGT 3 cut(s) 72, 432, 492
Hsp92I GRCGYC 2 cut(s) 253, 492
Hsp92II CATG 4 cut(s) 194, 224, 239, 381
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 4 cut(s) 6, 130, 205, 469
Lsp1109I GCAGC 3 cut(s) 50, 92, 174
LweI GCATC 1 cut(s) 391
MaeII ACGT 3 cut(s) 72, 432, 492
MaeIII GTNAC 2 cut(s) 391, 419
MalI GATC 4 cut(s) 8, 132, 207, 471
MboI GATC 4 cut(s) 6, 130, 205, 469
MboII GAAGA 1 cut(s) 77
MflI RGATCY 2 cut(s) 130, 205
MluCI AATT 3 cut(s) 16, 86, 115
MlyI GAGTC 1 cut(s) 454
MnlI CCTC 8 cut(s) 23, 60, 217, 268, 305, 330, 493, 537
MseI TTAA 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 438
MwoI GCNNNNNNNGC 1 cut(s) 521
NciI CCSGG 1 cut(s) 438
NdeII GATC 4 cut(s) 6, 130, 205, 469
NlaIII CATG 4 cut(s) 194, 224, 239, 381
NmuCI GTSAC 1 cut(s) 391
PagI TCATGA 1 cut(s) 220
PceI AGGCCT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 3 cut(s) 11, 66, 429
PfeI GAWTC 1 cut(s) 326
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 3 cut(s) 40, 82, 164
PleI GAGTC 1 cut(s) 454
PpsI GAGTC 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 201
PsuI RGATCY 2 cut(s) 130, 205
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SalI GTCGAC 1 cut(s) 494
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 3 cut(s) 39, 81, 163
Sau3AI GATC 4 cut(s) 6, 130, 205, 469
SchI GAGTC 1 cut(s) 454
ScrFI CCNGG 1 cut(s) 438
SfaNI GCATC 1 cut(s) 391
SgrDI CGTCGACG 1 cut(s) 494
Sse9I AATT 3 cut(s) 16, 86, 115
SseBI AGGCCT 1 cut(s) 319
SsiI CCGC 2 cut(s) 11, 199
StuI AGGCCT 1 cut(s) 319
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 4 cut(s) 103, 374, 391, 403
TaiI ACGT 3 cut(s) 75, 435, 495
TaqI TCGA 5 cut(s) 5, 60, 150, 369, 495
TaqII GACCGA 1 cut(s) 528
TasI AATT 3 cut(s) 16, 86, 115
TatI WGTACW 1 cut(s) 153
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 3 cut(s) 172, 379, 400
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 3 cut(s) 38, 80, 162
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 78, 343
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 3 cut(s) 172, 379, 400
Tth111I GACNNNGTC 1 cut(s) 275
XapI RAATTY 1 cut(s) 16
XmiI GTMKAC 2 cut(s) 279, 495
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.