Rorug06G0022100

Bifunctional phosphatase IMPL2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010954
Physical Location & Seq
Reverse (-)
2790373 .. 2791117
745 bp
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UTR
Exon/CDS
Intron
Rorug06G0022100.1

Sequence Viewer

Length: 279 bp
ATGCATCCTGATTGGAATAGTGACTGCACCGTGTATTGCAATCGTACAGGGAAGCTTGTGACATTGCCTTACAATGCTGCCATGCATGTCTCCCTCAAAGTGCTTGAACATGAGATTTTCACTGTGGGTCGCATCTGGGTTTTGGCACCTGCATCTACTGGTTCATACATCTCCGCCAAAGCCTCTGGTACTTCTATAGATGGTGGATCATGGTTGCTTTCTGAAGATCTGGCCAAGATGCTATTCCAAGCAAAGAATATGGTACCATCAAGTTTATAA

Protein Analysis

92

Amino Acids

10.03

Weight (kDa)

6.81

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 4 - 48 2.6e-06 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39120 AT4G39120
fragaria_vesca FvH4_2g09630 FvH4_2g09630 FvH4_2g09630 FvH4_2g09630
malus_domestica MD05G1061200.v1.1 MD10G1068900.v1.1
prunus_persica Prupe.8G090900_v2.0.a1
pyrus_communis pycom05g05220 pycom10g05500
rosa_chinensis RchiOBHm_Chr6g0265981
rosa_laevigata RLG00000014141
rosa_multiflora Rmu_sc0030533.1_g000003
rosa_roxburghii Rroxscaffold_7G00202170
rosa_rugosa Rorug06G0022100
rosa_samantha Rh6AG143300 Rh6BG143600 Rh6CG140300
rosa_wichuraiana Rw6G012390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 277
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 157
Acc65I GGTACC 1 cut(s) 262
AccB1I GGYRCC 2 cut(s) 145, 262
AciI CCGC 1 cut(s) 174
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 231
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 3 cut(s) 46, 190, 264
AgsI TTSAA 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 1 cut(s) 214
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 77
Asp718I GGTACC 1 cut(s) 262
BalI TGGCCA 1 cut(s) 233
BanI GGYRCC 2 cut(s) 145, 262
BbvI GCAGC 1 cut(s) 64
BccI CCATC 2 cut(s) 194, 274
BcoDI GTCTC 1 cut(s) 94
BfmI CTRYAG 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 157
BglII AGATCT 1 cut(s) 226
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 147, 264
BmsI GCATC 4 cut(s) 13, 141, 161, 228
Bse1I ACTGG 1 cut(s) 163
Bse3DI GCAATG 1 cut(s) 62
BseGI GGATG 1 cut(s) 4
BseMI GCAATG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 64
BsgI GTGCAG 1 cut(s) 10
BshFI GGCC 1 cut(s) 233
BshNI GGYRCC 2 cut(s) 145, 262
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 1 cut(s) 233
Bsp143I GATC 2 cut(s) 206, 226
BspACI CCGC 1 cut(s) 174
BspANI GGCC 1 cut(s) 233
BspLI GGNNCC 2 cut(s) 147, 264
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 2 cut(s) 145, 262
BsrDI GCAATG 1 cut(s) 62
BsrI ACTGG 1 cut(s) 163
BssMI GATC 2 cut(s) 206, 226
Bst4CI ACNGT 2 cut(s) 31, 124
BstF5I GGATG 1 cut(s) 4
BstKTI GATC 2 cut(s) 209, 229
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 2 cut(s) 206, 226
BstNSI RCATGY 1 cut(s) 89
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 1 cut(s) 233
BtsCI GGATG 1 cut(s) 4
BtsIMutI CAGTG 1 cut(s) 120
BveI ACCTGC 1 cut(s) 157
Csp6I GTAC 3 cut(s) 45, 189, 263
CviAII CATG 4 cut(s) 82, 86, 110, 210
CviJI RGCY 3 cut(s) 55, 182, 233
CviKI_1 RGCY 3 cut(s) 55, 182, 233
CviQI GTAC 3 cut(s) 45, 189, 263
DpnI GATC 2 cut(s) 208, 228
DpnII GATC 2 cut(s) 206, 226
EaeI YGGCCR 1 cut(s) 231
EciI GGCGGA 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 243
EcoT22I ATGCAT 2 cut(s) 6, 87
FaeI CATG 4 cut(s) 85, 89, 113, 213
FaiI YATR 8 cut(s) 83, 87, 111, 166, 197, 211, 260, 277
FatI CATG 4 cut(s) 81, 85, 109, 209
Fnu4HI GCNGC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 78
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 233
Hin1II CATG 4 cut(s) 85, 89, 113, 213
HindIII AAGCTT 1 cut(s) 53
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 1 cut(s) 8
HpyCH4III ACNGT 2 cut(s) 31, 124
HpyCH4V TGCA 5 cut(s) 4, 27, 39, 85, 152
Hsp92II CATG 4 cut(s) 85, 89, 113, 213
KpnI GGTACC 1 cut(s) 266
Kzo9I GATC 2 cut(s) 206, 226
LpnPI CCDG 7 cut(s) 21, 33, 121, 144, 162, 171, 215
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 4 cut(s) 13, 141, 161, 228
MaeIII GTNAC 2 cut(s) 20, 58
MalI GATC 2 cut(s) 208, 228
MboI GATC 2 cut(s) 206, 226
MboII GAAGA 1 cut(s) 236
MflI RGATCY 1 cut(s) 226
MlsI TGGCCA 1 cut(s) 233
MluNI TGGCCA 1 cut(s) 233
MnlI CCTC 2 cut(s) 104, 193
Mox20I TGGCCA 1 cut(s) 233
Mph1103I ATGCAT 2 cut(s) 6, 87
MscI TGGCCA 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 233
NdeII GATC 2 cut(s) 206, 226
NlaIII CATG 4 cut(s) 85, 89, 113, 213
NlaIV GGNNCC 2 cut(s) 147, 264
NmuCI GTSAC 2 cut(s) 20, 58
NsiI ATGCAT 2 cut(s) 6, 87
NspI RCATGY 1 cut(s) 89
PaqCI CACCTGC 1 cut(s) 157
PkrI GCNGC 1 cut(s) 79
PsiI TTATAA 1 cut(s) 277
PspN4I GGNNCC 2 cut(s) 147, 264
PsuI RGATCY 1 cut(s) 226
RsaI GTAC 3 cut(s) 46, 190, 264
RsaNI GTAC 3 cut(s) 45, 189, 263
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 2 cut(s) 206, 226
SetI ASST 2 cut(s) 57, 151
SfaNI GCATC 4 cut(s) 13, 141, 161, 228
SfcI CTRYAG 1 cut(s) 195
SsiI CCGC 1 cut(s) 174
TaaI ACNGT 2 cut(s) 31, 124
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 2 cut(s) 20, 58
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 2 cut(s) 20, 58
TspDTI ATGAA 1 cut(s) 153
TspRI CASTG 1 cut(s) 127
XceI RCATGY 1 cut(s) 89
Zsp2I ATGCAT 2 cut(s) 6, 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.