Rorug06G0034500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007791
Physical Location & Seq
Forward (+)
4223577 .. 4298069
74493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0034500.1

Sequence Viewer

Length: 3819 bp
ATGATTCTCGAGAATATTGAAGCCCAGAAAGATCAGCGATCAGCGGTCTTGGGAGGTCTTCTGTTCAACCGCCAAGCAGTGGAGTCAGAATCGAGGCCAGTCTCGGAGGCCTATTCCGGCACCGTCTTCTTTGTCTTCATCTCCTTCTCCAACCAAGAACAGTCTTGTTGTATTTCCGTCAACAGAAGGGTAGTTTGCTACGTCTACAAGCGGCCGGGGTCCGATTCAATTCCGAAGGAGATGGCGTCAAGCTCTTCTCGTCGTCAATGGAAGTACGATGTGTTTCTAAGTTTCAGAGGGCCAGATACGCGCAAGGGGATTACCATCGAGCTGTTTGATAGACTACAACGGAGAGGAGTTAAAACCTTCATGGATGACCGCGATCTGGAGGTAGGAGATTCCATTAGCCCGTCTCTTTTAGCTGCAATTGAGGAGTCGAGATTTGCCATTGTCATTCTTTCCCCCAACTATGCTTCTTCGTCATGGTGCTTGGAAGAACTGGTCAAGATTATTCAATGCATGAAAGAGAGGACGCTACGAGTCATGCCTCTGTTCTACAACGTGGAGCCTTCCGATGTGCAGCACCAAAGGGGAAGTTTTGAGCTAAAAAAGAAACCCCCACCCCAAGTAGACGTGGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGAATGAGGACCAATTCAAGGCGTGGAGAGCTGCTTTGACAGAGGTAGCCAATCTTTCTGGGTGGCATACACTGAATTATAGAACTGATAGAGAACTTGTTGAGACAATTGAGAATCAGGTGGTGTATAAATCTTCAAGTGTTGACAAGAGCTTCGTAGGAATGGATTTCCGTCTTGATGATTTATTACATAATTACATATATCAAGAGTTGGATGAGGTGCGAATTATAGGGATACATGGCATGCGAGGACTAGGTAAGACAACGGTTGCTCAAGCTTTTTATGATGACTTTTGTCAGGATTTTAAGCACAGATGCTTTCTTTTCAATGTTAGAGAAAGGTTTAAAAGGGATGGCCTAGTTTCTTTACAAAAGAACCTTCTTTCCAGCATCTTGATGGACAAATTTGAATACATAGAGGATGAATATACAGGAGCTGCTATGATTCAAAGAAGTTTATGTAAAATAAAGGTGCTAATTGTTATTGATGATGTGGATCAGTCGGCACAATTAGAAAAATTGGCTGGAAGTCGTGACTGGTTTGGTCCAGGGAGCAGAATTATCATAACCACCACAGATATTCACTTGTTAGAGGCACATGATGTTGACGCTCTATACAAGGCTACCGGGTTAAATCCTGGTGAAGCTCTTCAGCTCTTGAGTTTAAAGGCTTTTAAGAAAAGCTTCCCACCAGAAGATTATTTGGACCTGTGCGACCGTATTTTAGGGTATGCTCAGGGGCTGCCGTTAGCTCTTGTGGTTTTAGGTTCTTTTTTATGTGGTAGAAAGCACAATGAATGGGCAAGTGCAATTGATAGGCTAAAAAATACACCAAATAGAGGAATTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGACACAGAGAAAGAAATATTCCTACATATTGCTTGTTTTTACAAGGGGAAAGATAGGGATCGTGTGACACAAATACTAAACTATTGTGAGCTGAACCCTGTCATTGGATTAAGAGTTCTTGCTGATAAATCTCTCATTACTATTTCTAGCAATGAACTGTCAATGCATGATTTGCTACAAGAAATGGGCTGGGAAATAGTTCGTCGACAGTCTCCTAAAGAGTCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCCACCATGTGTTGAAGAAAAATAAGGGAACAGAAGCAATCCAAGGGATTGTAATGGAGTTGCCTAAATCAGAAGTGGCTTATTGGAATCCAGAAGCCTTCTCAAATTTGTCTGAACTTAATCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTGGAATGGCCTGGGTGTCCATTAAGATCTCTCCCACAAAAGTTCGAATCAGATGAACTTATGGAACTTAACTTGTGTCACAGCAATATTGAACATCTTTGGGAGGGAGAAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTTGAGAATCTGCCAGGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTGTCTAACTGCTCAAAAATTACAAAGATTCCCGAGTTTGATGGAAATATGGAGCGTCTGTCTGTGCTTGATCTCGACGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGGACGCCTGAGTGGCCTTGTGTCATTGAATCTAAGTAACTGCACAAATCTTGTCCGTCTTCCAAGCACCATCAATAATTTGAAGTTTGTTAAAAATCTTAATCTTTCTCGATGCATGAAACTTGACAAACAACAGGAAAGCGTGGGGGAGAGGGAATGTTATGAGGAAAATGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCTCATAAAGTATGTGAGAGGTTCAATCTTTCATGGATGGGAAGCAGTTTGGGGATCTTTAAATAAGTTCGGGCCTTCTGGATTGATGCAAAAAGTGATTACAGAGCAAATGAGTTTCCGCTTGCGTATATCTGGTCTCTGTAATTCAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCGACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCATCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTGAAGAGTTGTCAGACCTTCCATCAAATGGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACCGATTAAACGAATCATATTTCAGTTTCACGAATTGCGTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATATGATGGAAACTTCTCGAATTGCAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCGAAGTGTTGGGTATTCATTAAGTGTAGAGCTTCCTCCAGATTGGGATAGCAGTAGGTTTATGGGATTTGCTTTGTGTGTTGTTTTTGTACTCCATGAGCACCATCAGGTTGATGAGCGTGATATTCATCAATTCAAGACTTTTAATGCTACTCATCATCTTGTATGTTGCCTGAAGTTCAGTGGAAGAGAATTAGAAGTACACGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAGTTTTGCCAGCTGGCCTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGATTTCTTATTTAAAACCAGAGGGCCAGGTCAGGAGGTGAAGGAGTGTGGAGTTCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCCATATGAGGATATATTGGTTGATTTTGACATTCCAGTTGAAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACACTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1272

Amino Acids

145.0

Weight (kDa)

5.5

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 91 - 273 5.2e-49 TIR domain
TIR_2 PF13676 94 - 190 1e-16 TIR domain
NB-ARC PF00931 293 - 450 5.2e-16 NB-ARC domain
WHD_ROQ1 PF23282 517 - 587 3.5e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 776 - 862 7.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 787 - 859 5.1e-09 Leucine-rich repeat region
C-JID PF20160 1079 - 1228 7e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 2 cut(s) 79, 3023
AccI GTMKAC 4 cut(s) 204, 630, 1753, 3028
AccII CGCG 2 cut(s) 310, 381
AciI CCGC 5 cut(s) 44, 70, 211, 379, 2791
AclWI GGATC 3 cut(s) 1170, 1614, 2734
AcoI YGGCCR 1 cut(s) 212
AcsI RAATTY 5 cut(s) 1070, 1912, 2110, 2873, 3085
AcuI CTGAAG 5 cut(s) 1301, 1508, 2922, 3200, 3464
AcyI GRCGYC 2 cut(s) 245, 2461
AdeI CACNNNGTG 1 cut(s) 1816
AfaI GTAC 5 cut(s) 275, 3055, 3360, 3471, 3571
AfiI CCNNNNNNNGG 7 cut(s) 79, 385, 685, 1505, 1652, 3023, 3767
AjiI CACGTC 1 cut(s) 634
AjnI CCWGG 7 cut(s) 1213, 1303, 1995, 2005, 2308, 3238, 3628
AjuI GAANNNNNNNTTGG 2 cut(s) 2960, 2992
AloI GAACNNNNNNTCC 4 cut(s) 1034, 1066, 1647, 1679
Alw21I GWGCWC 1 cut(s) 3372
AlwI GGATC 3 cut(s) 1170, 1614, 2734
AlwNI CAGNNNCTG 2 cut(s) 1103, 1408
Ama87I CYCGRG 2 cut(s) 8, 2378
ApeKI GCWGC 5 cut(s) 422, 580, 698, 1103, 1408
ApoI RAATTY 5 cut(s) 1070, 1912, 2110, 2873, 3085
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AseI ATTAAT 1 cut(s) 2342
Asp700I GAANNNNTTC 4 cut(s) 1314, 1349, 1746, 3185
AspLEI GCGC 1 cut(s) 312
AspS9I GGNCC 8 cut(s) 219, 299, 676, 1211, 1372, 1951, 2744, 3626
AsuC2I CCSGG 2 cut(s) 216, 1294
AsuHPI GGTGA 5 cut(s) 1319, 2864, 2908, 3521, 3652
AsuII TTCGAA 1 cut(s) 2040
AvaI CYCGRG 2 cut(s) 8, 2378
AvaII GGWCC 5 cut(s) 219, 676, 1211, 1372, 1951
BanI GGYRCC 1 cut(s) 119
BauI CACGAG 1 cut(s) 3552
BbsI GAAGAC 6 cut(s) 50, 118, 127, 1809, 2507, 3188
Bbv12I GWGCWC 1 cut(s) 3372
BbvI GCAGC 5 cut(s) 409, 592, 685, 1090, 1395
BceAI ACGGC 2 cut(s) 1396, 3490
BcgI CGANNNNNNTGC 2 cut(s) 2411, 2445
BciT130I CCWGG 7 cut(s) 1215, 1305, 1997, 2007, 2310, 3240, 3630
BciVI GTATCC 1 cut(s) 894
BcnI CCSGG 2 cut(s) 216, 1294
BfaI CTAG 5 cut(s) 920, 1025, 1544, 1695, 3032
BfuI GTATCC 1 cut(s) 894
BglI GCCNNNNNGGC 1 cut(s) 2469
BglII AGATCT 2 cut(s) 2021, 2203
BisI GCNGC 6 cut(s) 212, 423, 581, 699, 1104, 1409
BlsI GCNGC 6 cut(s) 213, 424, 582, 700, 1105, 1410
Bme1390I CCNGG 9 cut(s) 216, 1215, 1294, 1305, 1997, 2007, 2310, 3240, 3630
Bme18I GGWCC 5 cut(s) 219, 676, 1211, 1372, 1951
BmeT110I CYCGRG 2 cut(s) 8, 2378
BmgBI CACGTC 1 cut(s) 634
BmgT120I GGNCC 8 cut(s) 219, 299, 676, 1211, 1372, 1951, 2744, 3626
BmiI GGNNCC 3 cut(s) 121, 220, 567
BmrFI CCNGG 9 cut(s) 216, 1215, 1294, 1305, 1997, 2007, 2310, 3240, 3630
BmsI GCATC 7 cut(s) 971, 1065, 2558, 2748, 3067, 3089, 3143
BoxI GACNNNNGTC 1 cut(s) 960
BpiI GAAGAC 6 cut(s) 50, 118, 127, 1809, 2507, 3188
BpmI CTGGAG 2 cut(s) 407, 3291
Bpu10I CCTNAGC 1 cut(s) 1401
Bpu14I TTCGAA 1 cut(s) 2040
BpuEI CTTGAG 6 cut(s) 924, 1345, 2008, 2213, 2318, 2984
BpuMI CCSGG 2 cut(s) 216, 1294
BsaBI GATNNNNATC 4 cut(s) 323, 1161, 1605, 3396
BsaHI GRCGYC 2 cut(s) 245, 2461
BsaI GGTCTC 3 cut(s) 1800, 2156, 2813
BsaJI CCNNGG 5 cut(s) 215, 1214, 1849, 2006, 2245
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 2390, 2420
Bsc4I CCNNNNNNNGG 7 cut(s) 79, 385, 685, 1505, 1652, 3023, 3767
Bse1I ACTGG 6 cut(s) 98, 504, 1208, 3761, 3777, 3790
Bse3DI GCAATG 1 cut(s) 1705
Bse8I GATNNNNATC 4 cut(s) 323, 1161, 1605, 3396
BseBI CCWGG 7 cut(s) 1215, 1305, 1997, 2007, 2310, 3240, 3630
BseDI CCNNGG 5 cut(s) 215, 1214, 1849, 2006, 2245
BseJI GATNNNNATC 4 cut(s) 323, 1161, 1605, 3396
BseLI CCNNNNNNNGG 7 cut(s) 79, 385, 685, 1505, 1652, 3023, 3767
BseMI GCAATG 1 cut(s) 1705
BseMII CTCAG 5 cut(s) 1415, 2456, 2825, 3246, 3537
BseNI ACTGG 6 cut(s) 98, 504, 1208, 3761, 3777, 3790
BseRI GAGGAG 2 cut(s) 369, 446
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 5 cut(s) 409, 592, 685, 1090, 1395
BseYI CCCAGC 1 cut(s) 1737
BsgI GTGCAG 2 cut(s) 599, 2482
Bsh1236I CGCG 2 cut(s) 310, 381
Bsh1285I CGRYCG 2 cut(s) 215, 1384
BshNI GGYRCC 1 cut(s) 119
BsiEI CGRYCG 2 cut(s) 215, 1384
BsiHKAI GWGCWC 1 cut(s) 3372
BsiHKCI CYCGRG 2 cut(s) 8, 2378
BsiSI CCGG 3 cut(s) 117, 215, 1293
BslI CCNNNNNNNGG 7 cut(s) 79, 385, 685, 1505, 1652, 3023, 3767
BsmBI CGTCTC 1 cut(s) 417
Bso31I GGTCTC 3 cut(s) 1800, 2156, 2813
BsoBI CYCGRG 2 cut(s) 8, 2378
Bsp119I TTCGAA 1 cut(s) 2040
Bsp1286I GDGCHC 1 cut(s) 3372
BspACI CCGC 5 cut(s) 44, 70, 211, 379, 2791
BspCNI CTCAG 5 cut(s) 1414, 2457, 2826, 3247, 3536
BspFNI CGCG 2 cut(s) 310, 381
BspHI TCATGA 1 cut(s) 1798
BspLI GGNNCC 3 cut(s) 121, 220, 567
BspPI GGATC 3 cut(s) 1170, 1614, 2734
BspQI GCTCTTC 2 cut(s) 259, 1320
BspT104I TTCGAA 1 cut(s) 2040
BspT107I GGYRCC 1 cut(s) 119
BspTNI GGTCTC 3 cut(s) 1800, 2156, 2813
BsrDI GCAATG 1 cut(s) 1705
BsrI ACTGG 6 cut(s) 98, 504, 1208, 3761, 3777, 3790
BssECI CCNNGG 5 cut(s) 215, 1214, 1849, 2006, 2245
BssNI GRCGYC 2 cut(s) 245, 2461
BssSI CACGAG 1 cut(s) 3552
BssT1I CCWWGG 2 cut(s) 1849, 2245
Bst2BI CACGAG 1 cut(s) 3552
Bst2UI CCWGG 7 cut(s) 1215, 1305, 1997, 2007, 2310, 3240, 3630
Bst4CI ACNGT 7 cut(s) 124, 162, 934, 1385, 1707, 1758, 3590
Bst6I CTCTTC 5 cut(s) 259, 1320, 2994, 3451, 3498
BstACI GRCGYC 2 cut(s) 245, 2461
BstAPI GCANNNNNTGC 3 cut(s) 1720, 3679, 3798
BstBI TTCGAA 1 cut(s) 2040
BstC8I GCNNGC 6 cut(s) 911, 2179, 2795, 3486, 3515, 3519
BstDEI CTNAG 9 cut(s) 287, 1401, 2465, 2489, 2834, 2909, 2958, 3255, 3523
BstEII GGTNACC 1 cut(s) 2896
BstFNI CGCG 2 cut(s) 310, 381
BstHHI GCGC 1 cut(s) 312
BstMCI CGRYCG 2 cut(s) 215, 1384
BstMWI GCNNNNNNNGC 8 cut(s) 307, 695, 1720, 2469, 2959, 3679, 3789, 3798
BstNI CCWGG 7 cut(s) 1215, 1305, 1997, 2007, 2310, 3240, 3630
BstNSI RCATGY 1 cut(s) 913
BstPAI GACNNNNGTC 1 cut(s) 960
BstPI GGTNACC 1 cut(s) 2896
BstSCI CCNGG 9 cut(s) 214, 1213, 1292, 1303, 1995, 2005, 2308, 3238, 3628
BstUI CGCG 2 cut(s) 310, 381
BstV1I GCAGC 5 cut(s) 409, 592, 685, 1090, 1395
BstV2I GAAGAC 6 cut(s) 50, 118, 127, 1809, 2507, 3188
BstX2I RGATCY 3 cut(s) 2021, 2203, 2726
BstYI RGATCY 3 cut(s) 2021, 2203, 2726
BstZI CGGCCG 1 cut(s) 212
BsuI GTATCC 1 cut(s) 894
BtrI CACGTC 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 6 cut(s) 84, 737, 3059, 3457, 3783, 3784
Cac8I GCNNGC 6 cut(s) 911, 2179, 2795, 3486, 3515, 3519
CaiI CAGNNNCTG 2 cut(s) 1103, 1408
CciI TCATGA 1 cut(s) 1798
CfoI GCGC 1 cut(s) 312
Cfr13I GGNCC 8 cut(s) 219, 299, 676, 1211, 1372, 1951, 2744, 3626
CseI GACGC 6 cut(s) 234, 541, 1283, 2390, 2469, 3118
Csp6I GTAC 5 cut(s) 274, 3054, 3359, 3470, 3570
CviQI GTAC 5 cut(s) 274, 3054, 3359, 3470, 3570
DdeI CTNAG 9 cut(s) 287, 1401, 2465, 2489, 2834, 2909, 2958, 3255, 3523
DraI TTTAAA 4 cut(s) 1012, 1332, 2733, 3616
DraIII CACNNNGTG 1 cut(s) 1816
EaeI YGGCCR 1 cut(s) 212
EagI CGGCCG 1 cut(s) 212
Eam1104I CTCTTC 5 cut(s) 259, 1320, 2994, 3451, 3498
EarI CTCTTC 5 cut(s) 259, 1320, 2994, 3451, 3498
EclXI CGGCCG 1 cut(s) 212
Eco130I CCWWGG 2 cut(s) 1849, 2245
Eco147I AGGCCT 2 cut(s) 110, 1965
Eco31I GGTCTC 3 cut(s) 1800, 2156, 2813
Eco47I GGWCC 5 cut(s) 219, 676, 1211, 1372, 1951
Eco52I CGGCCG 1 cut(s) 212
Eco57I CTGAAG 5 cut(s) 1301, 1508, 2922, 3200, 3464
Eco88I CYCGRG 2 cut(s) 8, 2378
Eco91I GGTNACC 1 cut(s) 2896
EcoO65I GGTNACC 1 cut(s) 2896
EcoRII CCWGG 7 cut(s) 1213, 1303, 1995, 2005, 2308, 3238, 3628
EcoT14I CCWWGG 2 cut(s) 1849, 2245
EcoT22I ATGCAT 4 cut(s) 521, 1716, 2573, 3082
ErhI CCWWGG 2 cut(s) 1849, 2245
Esp3I CGTCTC 1 cut(s) 417
FalI AAGNNNNNCTT 2 cut(s) 1334, 1366
FauNDI CATATG 1 cut(s) 3730
FblI GTMKAC 4 cut(s) 204, 630, 1753, 3028
Fnu4HI GCNGC 6 cut(s) 212, 423, 581, 699, 1104, 1409
Fsp4HI GCNGC 6 cut(s) 212, 423, 581, 699, 1104, 1409
FspBI CTAG 5 cut(s) 920, 1025, 1544, 1695, 3032
GlaI GCGC 1 cut(s) 311
GluI GCNGC 6 cut(s) 212, 423, 581, 699, 1104, 1409
GsaI CCCAGC 1 cut(s) 1741
GsuI CTGGAG 2 cut(s) 407, 3291
HapII CCGG 3 cut(s) 117, 215, 1293
HgaI GACGC 6 cut(s) 234, 541, 1283, 2390, 2469, 3118
HhaI GCGC 1 cut(s) 312
Hin1I GRCGYC 2 cut(s) 245, 2461
Hin6I GCGC 1 cut(s) 310
HinP1I GCGC 1 cut(s) 310
HincII GTYRAC 4 cut(s) 181, 811, 1273, 1754
HindII GTYRAC 4 cut(s) 181, 811, 1273, 1754
HindIII AAGCTT 4 cut(s) 942, 1348, 2261, 2960
HpaII CCGG 3 cut(s) 117, 215, 1293
HphI GGTGA 5 cut(s) 1319, 2864, 2908, 3521, 3652
Hpy166II GTNNAC 9 cut(s) 181, 205, 631, 811, 1273, 1754, 1951, 3029, 3472
Hpy8I GTNNAC 9 cut(s) 181, 205, 631, 811, 1273, 1754, 1951, 3029, 3472
Hpy99I CGWCG 4 cut(s) 264, 1755, 2426, 2873
HpyCH4III ACNGT 7 cut(s) 124, 162, 934, 1385, 1707, 1758, 3590
HpyCH4IV ACGT 4 cut(s) 201, 561, 633, 1780
HpyF10VI GCNNNNNNNGC 8 cut(s) 307, 695, 1720, 2469, 2959, 3679, 3789, 3798
HpyF3I CTNAG 9 cut(s) 287, 1401, 2465, 2489, 2834, 2909, 2958, 3255, 3523
HpySE526I ACGT 4 cut(s) 201, 561, 633, 1780
Hsp92I GRCGYC 2 cut(s) 245, 2461
HspAI GCGC 1 cut(s) 310
LguI GCTCTTC 2 cut(s) 259, 1320
LmnI GCTCC 5 cut(s) 565, 1100, 1218, 2398, 2858
Lsp1109I GCAGC 5 cut(s) 409, 592, 685, 1090, 1395
LweI GCATC 7 cut(s) 971, 1065, 2558, 2748, 3067, 3089, 3143
MaeI CTAG 5 cut(s) 920, 1025, 1544, 1695, 3032
MaeII ACGT 4 cut(s) 201, 561, 633, 1780
MaeIII GTNAC 6 cut(s) 1199, 1612, 2072, 2492, 2879, 2896
MfeI CAATTG 4 cut(s) 426, 774, 1476, 2454
MflI RGATCY 3 cut(s) 2021, 2203, 2726
MhlI GDGCHC 1 cut(s) 3372
MlyI GAGTC 5 cut(s) 92, 443, 549, 1778, 1986
MmeI TCCRAC 2 cut(s) 174, 858
Mph1103I ATGCAT 4 cut(s) 521, 1716, 2573, 3082
MroXI GAANNNNTTC 4 cut(s) 1314, 1349, 1746, 3185
MslI CAYNNNNRTG 2 cut(s) 1061, 3273
MspA1I CMGCKG 2 cut(s) 44, 3517
MspI CCGG 3 cut(s) 117, 215, 1293
MspR9I CCNGG 9 cut(s) 216, 1215, 1294, 1305, 1997, 2007, 2310, 3240, 3630
MunI CAATTG 4 cut(s) 426, 774, 1476, 2454
MvaI CCWGG 7 cut(s) 1215, 1305, 1997, 2007, 2310, 3240, 3630
MvnI CGCG 2 cut(s) 310, 381
MwoI GCNNNNNNNGC 8 cut(s) 307, 695, 1720, 2469, 2959, 3679, 3789, 3798
NciI CCSGG 2 cut(s) 216, 1294
NdeI CATATG 1 cut(s) 3730
NlaIV GGNNCC 3 cut(s) 121, 220, 567
NmuCI GTSAC 4 cut(s) 1199, 1612, 2072, 2896
NsiI ATGCAT 4 cut(s) 521, 1716, 2573, 3082
NspI RCATGY 1 cut(s) 913
NspV TTCGAA 1 cut(s) 2040
PaeI GCATGC 1 cut(s) 913
PaeR7I CTCGAG 1 cut(s) 8
PagI TCATGA 1 cut(s) 1798
PceI AGGCCT 2 cut(s) 110, 1965
PciSI GCTCTTC 2 cut(s) 259, 1320
PdmI GAANNNNTTC 4 cut(s) 1314, 1349, 1746, 3185
PflMI CCANNNNNTGG 2 cut(s) 79, 3023
PfoI TCCNGGA 2 cut(s) 1995, 3238
PkrI GCNGC 6 cut(s) 213, 424, 582, 700, 1105, 1410
PleI GAGTC 5 cut(s) 91, 442, 548, 1777, 1986
PpsI GAGTC 5 cut(s) 91, 442, 548, 1777, 1986
PshAI GACNNNNGTC 1 cut(s) 960
PshBI ATTAAT 1 cut(s) 2342
Psp6I CCWGG 7 cut(s) 1213, 1303, 1995, 2005, 2308, 3238, 3628
PspEI GGTNACC 1 cut(s) 2896
PspFI CCCAGC 1 cut(s) 1737
PspGI CCWGG 7 cut(s) 1213, 1303, 1995, 2005, 2308, 3238, 3628
PspN4I GGNNCC 3 cut(s) 121, 220, 567
PspPI GGNCC 8 cut(s) 219, 299, 676, 1211, 1372, 1951, 2744, 3626
PstNI CAGNNNCTG 2 cut(s) 1103, 1408
PsuI RGATCY 3 cut(s) 2021, 2203, 2726
PvuII CAGCTG 1 cut(s) 3517
RsaI GTAC 5 cut(s) 275, 3055, 3360, 3471, 3571
RsaNI GTAC 5 cut(s) 274, 3054, 3359, 3470, 3570
RseI CAYNNNNRTG 2 cut(s) 1061, 3273
SalI GTCGAC 1 cut(s) 1752
SapI GCTCTTC 2 cut(s) 259, 1320
SatI GCNGC 6 cut(s) 212, 423, 581, 699, 1104, 1409
Sau96I GGNCC 8 cut(s) 219, 299, 676, 1211, 1372, 1951, 2744, 3626
SchI GAGTC 5 cut(s) 92, 443, 549, 1778, 1986
ScrFI CCNGG 9 cut(s) 216, 1215, 1294, 1305, 1997, 2007, 2310, 3240, 3630
SduI GDGCHC 1 cut(s) 3372
SfaNI GCATC 7 cut(s) 971, 1065, 2558, 2748, 3067, 3089, 3143
Sfr274I CTCGAG 1 cut(s) 8
SfuI TTCGAA 1 cut(s) 2040
SinI GGWCC 5 cut(s) 219, 676, 1211, 1372, 1951
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 1061, 3273
SmlI CTYRAG 7 cut(s) 8, 939, 1324, 1987, 2192, 2297, 2963
SmoI CTYRAG 7 cut(s) 8, 939, 1324, 1987, 2192, 2297, 2963
SphI GCATGC 1 cut(s) 913
SseBI AGGCCT 2 cut(s) 110, 1965
SsiI CCGC 5 cut(s) 44, 70, 211, 379, 2791
SspI AATATT 4 cut(s) 16, 1566, 2083, 3250
SspMI CTAG 5 cut(s) 920, 1025, 1544, 1695, 3032
StuI AGGCCT 2 cut(s) 110, 1965
StyD4I CCNGG 9 cut(s) 214, 1213, 1292, 1303, 1995, 2005, 2308, 3238, 3628
StyI CCWWGG 2 cut(s) 1849, 2245
TaaI ACNGT 7 cut(s) 124, 162, 934, 1385, 1707, 1758, 3590
TaiI ACGT 4 cut(s) 204, 564, 636, 1783
TatI WGTACW 3 cut(s) 3053, 3358, 3469
TauI GCSGC 1 cut(s) 214
TscAI CASTG 6 cut(s) 84, 744, 3066, 3457, 3784, 3790
TseFI GTSAC 4 cut(s) 1199, 1612, 2072, 2896
TseI GCWGC 5 cut(s) 422, 580, 698, 1103, 1408
Tsp45I GTSAC 4 cut(s) 1199, 1612, 2072, 2896
TspGWI ACGGA 4 cut(s) 166, 364, 827, 2501
TspRI CASTG 6 cut(s) 84, 744, 3066, 3457, 3784, 3790
Van91I CCANNNNNTGG 2 cut(s) 79, 3023
VpaK11BI GGWCC 5 cut(s) 219, 676, 1211, 1372, 1951
VspI ATTAAT 1 cut(s) 2342
XapI RAATTY 5 cut(s) 1070, 1912, 2110, 2873, 3085
XceI RCATGY 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 3 cut(s) 1060, 2243, 3275
XhoI CTCGAG 1 cut(s) 8
XmiI GTMKAC 4 cut(s) 204, 630, 1753, 3028
XmnI GAANNNNTTC 4 cut(s) 1314, 1349, 1746, 3185
XspI CTAG 5 cut(s) 920, 1025, 1544, 1695, 3032
Zsp2I ATGCAT 4 cut(s) 521, 1716, 2573, 3082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.