Rorug06G0312800

Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006681
Physical Location & Seq
Reverse (-)
46438065 .. 46438499
435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0312800.1

Sequence Viewer

Length: 435 bp
ATGGACGAGAAGAAGACTCTGGAGTCTCACGATCTGTTGTCTAGGTTCTTGAGCTCAGGCCACTCCGACGAGAAATTTGTCACTGACATCGTCATCAGCTTCACTCTTGCCGGAAGGGACACGACCTCGGCGGCTTTGACCTGGTTCTTCTGGCTAATATCAAACCATATTCTTAAGGAGATCAATGAGAAAACGGAGACCGGAGCCAGATATGATGAAGCGAAGGATATGGTGTACACACACGCGGCGCTCTGTGAGAGCATGAGGTTGTACCCGCCTGTTCCGACTGACAACAAAGAGGCGGTAGAAGACGACGTTTTTCCGAACGGGACTAAAGTGAAGAATGGGACGACAGTGACGTACCACGTGTACGCGATGGGGCTGATGGAGCAGATATGGGGCAAGGATTGGGCCGAGTTCCGCCCCGAGAGGTAG

Protein Analysis

144

Amino Acids

16.53

Weight (kDa)

5.06

Isoelectric Point (pI)

25.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 23 - 144 1.2e-19 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccII CGCG 2 cut(s) 245, 374
AciI CCGC 5 cut(s) 131, 245, 275, 302, 421
AcsI RAATTY 1 cut(s) 74
AcvI CACGTG 1 cut(s) 367
AfaI GTAC 4 cut(s) 236, 272, 362, 371
AflII CTTAAG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 366
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 2 cut(s) 54, 99
AluI AGCT 2 cut(s) 54, 99
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 30, 191
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 2 cut(s) 58, 411
ApoI RAATTY 1 cut(s) 74
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 411
AvaI CYCGRG 1 cut(s) 425
BanII GRGCYC 1 cut(s) 56
BbrPI CACGTG 1 cut(s) 367
BbsI GAAGAC 2 cut(s) 20, 315
Bbv12I GWGCWC 1 cut(s) 56
BccI CCATC 2 cut(s) 370, 379
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 30, 191
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 251
BfrI CTTAAG 1 cut(s) 173
BisI GCNGC 2 cut(s) 132, 246
BlsI GCNGC 2 cut(s) 133, 247
Bme1390I CCNGG 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 142
BpiI GAAGAC 2 cut(s) 20, 315
BpmI CTGGAG 1 cut(s) 41
Bpu10I CCTNAGC 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 70
BsaAI YACGTR 1 cut(s) 367
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 200
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 126
BseMII CTCAG 1 cut(s) 69
Bsh1236I CGCG 2 cut(s) 245, 374
BshFI GGCC 2 cut(s) 60, 413
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 2 cut(s) 111, 201
BslFI GGGAC 3 cut(s) 131, 343, 361
BsmAI GTCTC 2 cut(s) 30, 191
BsmFI GGGAC 3 cut(s) 131, 343, 361
BsnI GGCC 2 cut(s) 60, 413
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 2 cut(s) 31, 180
BspACI CCGC 5 cut(s) 131, 245, 275, 302, 421
BspANI GGCC 2 cut(s) 60, 413
BspCNI CTCAG 1 cut(s) 68
BspFNI CGCG 2 cut(s) 245, 374
BspLI GGNNCC 1 cut(s) 205
BspTI CTTAAG 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 191
BsrGI TGTACA 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 2 cut(s) 31, 180
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 355
BstAFI CTTAAG 1 cut(s) 173
BstAUI TGTACA 1 cut(s) 234
BstBAI YACGTR 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 55
BstFNI CGCG 2 cut(s) 245, 374
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 2 cut(s) 34, 183
BstMAI GTCTC 2 cut(s) 30, 191
BstMBI GATC 2 cut(s) 31, 180
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstUI CGCG 2 cut(s) 245, 374
BstV2I GAAGAC 2 cut(s) 20, 315
BsuRI GGCC 2 cut(s) 60, 413
BtgZI GCGATG 1 cut(s) 389
BtsIMutI CAGTG 2 cut(s) 81, 360
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 411
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 4 cut(s) 235, 271, 361, 370
CviAII CATG 1 cut(s) 262
CviJI RGCY 8 cut(s) 54, 60, 99, 134, 154, 206, 382, 413
CviKI_1 RGCY 8 cut(s) 54, 60, 99, 134, 154, 206, 382, 413
CviQI GTAC 4 cut(s) 235, 271, 361, 370
DdeI CTNAG 1 cut(s) 55
DpnI GATC 2 cut(s) 33, 182
DpnII GATC 2 cut(s) 31, 180
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EciI GGCGGA 1 cut(s) 410
Ecl136II GAGCTC 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 56
Eco31I GGTCTC 1 cut(s) 191
Eco53kI GAGCTC 1 cut(s) 54
Eco72I CACGTG 1 cut(s) 367
Eco88I CYCGRG 1 cut(s) 425
EcoICRI GAGCTC 1 cut(s) 54
EcoRII CCWGG 1 cut(s) 140
EcoT38I GRGCYC 1 cut(s) 56
FaeI CATG 1 cut(s) 265
FaiI YATR 5 cut(s) 168, 213, 230, 263, 397
FaqI GGGAC 3 cut(s) 131, 343, 361
FatI CATG 1 cut(s) 261
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 2 cut(s) 132, 246
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 2 cut(s) 132, 246
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 249
GluI GCNGC 2 cut(s) 132, 246
GsuI CTGGAG 1 cut(s) 41
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 2 cut(s) 60, 413
HapII CCGG 2 cut(s) 111, 201
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 2 cut(s) 16, 23
HpaII CCGG 2 cut(s) 111, 201
Hpy166II GTNNAC 3 cut(s) 235, 237, 370
Hpy188I TCNGA 3 cut(s) 67, 285, 324
Hpy188III TCNNGA 3 cut(s) 20, 29, 49
Hpy8I GTNNAC 3 cut(s) 235, 237, 370
Hpy99I CGWCG 2 cut(s) 71, 317
HpyAV CCTTC 2 cut(s) 108, 217
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 3 cut(s) 315, 359, 366
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 1 cut(s) 55
HpySE526I ACGT 3 cut(s) 315, 359, 366
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 2 cut(s) 31, 180
LmnI GCTCC 2 cut(s) 203, 388
LpnPI CCDG 9 cut(s) 5, 42, 124, 127, 136, 154, 214, 220, 291
MabI ACCWGGT 1 cut(s) 140
MaeI CTAG 1 cut(s) 42
MaeII ACGT 3 cut(s) 315, 359, 366
MaeIII GTNAC 2 cut(s) 79, 355
MalI GATC 2 cut(s) 33, 182
MboI GATC 2 cut(s) 31, 180
MboII GAAGA 5 cut(s) 22, 25, 139, 320, 352
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 1 cut(s) 74
MlyI GAGTC 2 cut(s) 10, 32
MmeI TCCRAC 2 cut(s) 90, 308
MnlI CCTC 4 cut(s) 136, 258, 292, 423
MseI TTAA 1 cut(s) 174
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 2 cut(s) 111, 201
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 2 cut(s) 245, 374
MwoI GCNNNNNNNGC 1 cut(s) 388
NdeII GATC 2 cut(s) 31, 180
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 1 cut(s) 205
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 2 cut(s) 79, 355
PcsI WCGNNNNNNNCGW 1 cut(s) 356
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 133, 247
PleI GAGTC 2 cut(s) 10, 31
PmaCI CACGTG 1 cut(s) 367
PmlI CACGTG 1 cut(s) 367
PpsI GAGTC 2 cut(s) 10, 31
Ppu21I YACGTR 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 140
PspCI CACGTG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 411
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 4 cut(s) 236, 272, 362, 371
RsaNI GTAC 4 cut(s) 235, 271, 361, 370
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 2 cut(s) 132, 246
Sau3AI GATC 2 cut(s) 31, 180
Sau96I GGNCC 1 cut(s) 411
SchI GAGTC 2 cut(s) 10, 32
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 56
SexAI ACCWGGT 1 cut(s) 140
SmlI CTYRAG 2 cut(s) 49, 173
SmoI CTYRAG 2 cut(s) 49, 173
Sse9I AATT 1 cut(s) 74
SsiI CCGC 5 cut(s) 131, 245, 275, 302, 421
SspMI CTAG 1 cut(s) 42
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 3 cut(s) 318, 362, 369
TasI AATT 1 cut(s) 74
TatI WGTACW 1 cut(s) 234
TauI GCSGC 2 cut(s) 134, 248
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 2 cut(s) 88, 360
TseFI GTSAC 2 cut(s) 79, 355
Tsp45I GTSAC 2 cut(s) 79, 355
TspDTI ATGAA 1 cut(s) 231
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 2 cut(s) 88, 360
Tth111I GACNNNGTC 1 cut(s) 89
Vha464I CTTAAG 1 cut(s) 173
XapI RAATTY 1 cut(s) 74
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.