Rorug06G0323100

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007063
Physical Location & Seq
Forward (+)
47442868 .. 47443647
780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0323100.1

Sequence Viewer

Length: 498 bp
ATGGATCATCACCCCCTTCACAACACCCACGTAAAGCTCTTAGCTTTCGACTTGCTCACTCTCGCCCAAACCTCCTCCTCCGACCCAACCTCCTTCTCCCGCAACGGCCTCCTCCTCTCACGGGCCGAGGCCGTCGGCACCGTGACCTCACGCGACCTCAAACCCAACAAGTTCCTCCGCTTCACCATCGACGACGGCACCGGCTGCGTCAGCTGCATTCTCTGGCTCAACCACCTCACCTCCCCTTACTTCTCCCGACGAAGCCCACCAGATGTTCGACTCATTGCCTCAATGGCCGCCCGTTTCGCCGCCGACGTCAGCCTCGGAGCAGTGGCCAGAGTGCGCGGGAAAATCTCGAGCTTCAGGGGTGAAATGCAGATCACTGTGACCGATGTTGTGATCGAGAGGGACCCGAATGCGGAGATGTTGCATTGGATTGAGTGCATGAGGTTGGCGCGGAAGGTTTACAATGTGGTTGCTACTACTCCTTCCAAATAG

Protein Analysis

165

Amino Acids

18.32

Weight (kDa)

9.45

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 45 - 133 1e-08 OB-fold nucleic acid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 318
AccB1I GGYRCC 2 cut(s) 137, 197
AccII CGCG 3 cut(s) 153, 345, 457
AciI CCGC 7 cut(s) 100, 178, 297, 309, 345, 419, 457
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 294, 333
AcuI CTGAAG 1 cut(s) 346
AcyI GRCGYC 1 cut(s) 315
AfiI CCNNNNNNNGG 2 cut(s) 121, 418
AluBI AGCT 4 cut(s) 37, 44, 213, 360
AluI AGCT 4 cut(s) 37, 44, 213, 360
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 355
AoxI GGCC 5 cut(s) 106, 123, 129, 294, 333
ApeKI GCWGC 2 cut(s) 204, 213
AspLEI GCGC 2 cut(s) 345, 457
AspS9I GGNCC 2 cut(s) 123, 409
AsuHPI GGTGA 3 cut(s) 175, 229, 380
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 1 cut(s) 409
BalI TGGCCA 1 cut(s) 335
BanI GGYRCC 2 cut(s) 137, 197
BbvI GCAGC 2 cut(s) 191, 200
BccI CCATC 1 cut(s) 194
BceAI ACGGC 3 cut(s) 116, 121, 211
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 4 cut(s) 205, 214, 297, 309
BlsI GCNGC 4 cut(s) 206, 215, 298, 310
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 1 cut(s) 355
BmgT120I GGNCC 2 cut(s) 123, 409
BmiI GGNNCC 4 cut(s) 139, 199, 410, 411
BsaAI YACGTR 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 126, 322
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 224, 254
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 418
Bse118I RCCGGY 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 282
BseDI CCNNGG 2 cut(s) 126, 322
BseLI CCNNNNNNNGG 2 cut(s) 121, 418
BseMI GCAATG 1 cut(s) 282
BseRI GAGGAG 4 cut(s) 64, 67, 101, 104
BseXI GCAGC 2 cut(s) 191, 200
Bsh1236I CGCG 3 cut(s) 153, 345, 457
BshFI GGCC 5 cut(s) 108, 125, 131, 296, 335
BshNI GGYRCC 2 cut(s) 137, 197
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 2 cut(s) 121, 418
BsmFI GGGAC 1 cut(s) 422
BsmI GAATGC 2 cut(s) 216, 421
BsnI GGCC 5 cut(s) 108, 125, 131, 296, 335
BsoBI CYCGRG 1 cut(s) 355
Bsp143I GATC 3 cut(s) 4, 378, 399
BspACI CCGC 7 cut(s) 100, 178, 297, 309, 345, 419, 457
BspANI GGCC 5 cut(s) 108, 125, 131, 296, 335
BspFNI CGCG 3 cut(s) 153, 345, 457
BspLI GGNNCC 4 cut(s) 139, 199, 410, 411
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 137, 197
BsrDI GCAATG 1 cut(s) 282
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 126, 322
BssMI GATC 3 cut(s) 4, 378, 399
BssNI GRCGYC 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 142, 385
BstACI GRCGYC 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 40
BstFNI CGCG 3 cut(s) 153, 345, 457
BstHHI GCGC 2 cut(s) 345, 457
BstKTI GATC 3 cut(s) 7, 381, 402
BstMBI GATC 3 cut(s) 4, 378, 399
BstMWI GCNNNNNNNGC 5 cut(s) 204, 210, 213, 293, 305
BstUI CGCG 3 cut(s) 153, 345, 457
BstV1I GCAGC 2 cut(s) 191, 200
BsuRI GGCC 5 cut(s) 108, 125, 131, 296, 335
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 336, 381
CfoI GCGC 2 cut(s) 345, 457
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 123, 409
CseI GACGC 1 cut(s) 196
CviAII CATG 1 cut(s) 445
DdeI CTNAG 1 cut(s) 40
DpnI GATC 3 cut(s) 6, 380, 401
DpnII GATC 3 cut(s) 4, 378, 399
EaeI YGGCCR 2 cut(s) 294, 333
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 346
Eco88I CYCGRG 1 cut(s) 355
EcoO109I RGGNCCY 1 cut(s) 409
FaeI CATG 1 cut(s) 448
FaiI YATR 1 cut(s) 446
FaqI GGGAC 1 cut(s) 422
FatI CATG 1 cut(s) 444
FauI CCCGC 2 cut(s) 107, 338
Fnu4HI GCNGC 4 cut(s) 205, 214, 297, 309
Fsp4HI GCNGC 4 cut(s) 205, 214, 297, 309
GlaI GCGC 2 cut(s) 344, 456
GluI GCNGC 4 cut(s) 205, 214, 297, 309
HaeIII GGCC 5 cut(s) 108, 125, 131, 296, 335
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 196
HhaI GCGC 2 cut(s) 345, 457
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 1 cut(s) 448
Hin6I GCGC 2 cut(s) 343, 455
HinP1I GCGC 2 cut(s) 343, 455
HinfI GANTC 1 cut(s) 279
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 175, 229, 380
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 2 cut(s) 82, 326
Hpy188III TCNNGA 3 cut(s) 255, 355, 403
Hpy8I GTNNAC 1 cut(s) 466
Hpy99I CGWCG 5 cut(s) 137, 194, 197, 261, 317
HpyAV CCTTC 4 cut(s) 26, 103, 454, 498
HpyCH4III ACNGT 2 cut(s) 142, 385
HpyCH4IV ACGT 2 cut(s) 30, 315
HpyCH4V TGCA 4 cut(s) 216, 376, 430, 444
HpyF10VI GCNNNNNNNGC 5 cut(s) 204, 210, 213, 293, 305
HpyF3I CTNAG 1 cut(s) 40
HpySE526I ACGT 2 cut(s) 30, 315
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 1 cut(s) 448
HspAI GCGC 2 cut(s) 343, 455
KflI GGGWCCC 1 cut(s) 409
Kzo9I GATC 3 cut(s) 4, 378, 399
LmnI GCTCC 1 cut(s) 326
LpnPI CCDG 5 cut(s) 208, 214, 282, 349, 349
Lsp1109I GCAGC 2 cut(s) 191, 200
MaeII ACGT 2 cut(s) 30, 315
MaeIII GTNAC 2 cut(s) 142, 385
MalI GATC 3 cut(s) 6, 380, 401
MboI GATC 3 cut(s) 4, 378, 399
MlsI TGGCCA 1 cut(s) 335
MluNI TGGCCA 1 cut(s) 335
MlyI GAGTC 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 105
Mox20I TGGCCA 1 cut(s) 335
MscI TGGCCA 1 cut(s) 335
Msp20I TGGCCA 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 1 cut(s) 201
Mva1269I GAATGC 2 cut(s) 216, 421
MvnI CGCG 3 cut(s) 153, 345, 457
MwoI GCNNNNNNNGC 5 cut(s) 204, 210, 213, 293, 305
NdeII GATC 3 cut(s) 4, 378, 399
NlaIII CATG 1 cut(s) 448
NlaIV GGNNCC 4 cut(s) 139, 199, 410, 411
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 142, 385
PaeR7I CTCGAG 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 2 cut(s) 216, 421
PkrI GCNGC 4 cut(s) 206, 215, 298, 310
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
Ppu21I YACGTR 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 409
PspN4I GGNNCC 4 cut(s) 139, 199, 410, 411
PspPI GGNCC 2 cut(s) 123, 409
PspPPI RGGWCCY 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 213
SatI GCNGC 4 cut(s) 205, 214, 297, 309
Sau3AI GATC 3 cut(s) 4, 378, 399
Sau96I GGNCC 2 cut(s) 123, 409
SchI GAGTC 1 cut(s) 273
Sfr274I CTCGAG 1 cut(s) 355
SinI GGWCC 1 cut(s) 409
SlaI CTCGAG 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
SsiI CCGC 7 cut(s) 100, 178, 297, 309, 345, 419, 457
TaaI ACNGT 2 cut(s) 142, 385
TaiI ACGT 2 cut(s) 33, 318
TaqI TCGA 5 cut(s) 48, 189, 277, 356, 402
TaqII GACCGA 1 cut(s) 404
TauI GCSGC 2 cut(s) 299, 311
TscAI CASTG 2 cut(s) 336, 388
TseFI GTSAC 2 cut(s) 142, 385
TseI GCWGC 2 cut(s) 204, 213
Tsp45I GTSAC 2 cut(s) 142, 385
TspRI CASTG 2 cut(s) 336, 388
VpaK11BI GGWCC 1 cut(s) 409
XhoI CTCGAG 1 cut(s) 355
ZraI GACGTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.