Rorug06G0402100

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007421
Physical Location & Seq
Forward (+)
54665325 .. 54676934
11610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0402100.1

Sequence Viewer

Length: 4125 bp
ATGAGTAGCTTGGTTGAGAGGCTGCGCACTCGATCAGACCGAAAGCCAATTTATAATATTGACGAGTCTGACGATGATGGTGATTTTGGGACTGGAAAACCTGGAAGTGCTCAGGAAAAGTTTGAGAAGATTGTTAGGACTGATGTGAAAGATAATTCCTGTCAAGCGTGTGGGGAAAGCGAGAATCTTATGGAATGTGAGACATGCAACTATGCATATCATGCTAAGTGTTTACTTCCCCCGTTAAAGGCTCCTCTCCCTGACAGCTGGCGATGTCCGGAATGTGTGAGCCCTCTGACTGACATCGACAAGATTCTGGATTGTGAAATGCGACCTACTGTGGCCGATGATAGTGACGCCTCAAAGCTGGGTTCAAAGCAAATTTTTGTTAAACAATATCTTGTGAAGTGGAAGGGATTATCATATCTACATTGCACATGGATTCCTGAGAAAGAATTTTTCAAAGCGTTCAAGACGTACCCACGTCTAAAGCAGAAGGTTAACAATTTCAATCGTCAAAGTTCTTCAAATAATAACACAGATGATGAATTTGTTGCCATTCGACCCGAATGGACAACTGTTGATCGGATCCTTGCTTGCAGGGGCGACGATGAGAAGCAATATCTTGTAAAGTGGAAAGAACTTCCTTACGATGAATGCTATTGGGAGGTGGAGGCTGATATCTCGACATTTCAACCAGAAATAGAGAGATTCCATAAAATACAGTCTAGATCTCAGAAATTGTCTGGTAAGCAGAAGAGTAGTCTTAAAGATGCTCTCGAACCAAAAAAGAAGCAGAAAGAGTTTCAACAGTTCGAACACAGTCCTGAATTTCTTTCTGGAGGTACACTGCATCCCTATCAGCTTGAAGGTTTGAACTTCTTACGTTTCTCCTGGTCCAAACAAACTCATGTCATACTTGCAGACGAGATGGGACTTGGCAAAACTATACAGAGTATAGCTTTTTTAGCATCTCTCTTTGAAGAGAGAGTCTATCCACATCTAGTGGTGGCTCCACTTTCAACATTACGAAATTGGGAACGGGAATTTGCAACATGGGCACCTCAAATGAATGTTGTTATGTATGTTGGCTCTTCCCAAGCCCGTGCGGTCATACGTGAATACGAATTTTACTTCCCCAAAAATCACAAGAAGATCAAGAAAAAGAAATCTGGTCAGATCAGTGGGGAAAGCAAGCAAGATAGGATCAAGTTTGATGTCCTTCTCACATCATATGAGATGATTAACTTGGACTCTACATCACTGAAACCGATAAAGTGGGACTGTATGATTGTTGATGAAGGCCATCGTCTCAAAAACAAGGATTCAAAATTGTTTTCATCATTACAGCAGTACTGGACTAATCATCGCACATTGCTGACTGGAACTCCTCTTCAGAACAATCTGGACGAACTTTTCATGCTTATGCACTTTCTCGATGCTGCCAAGTTTGCAAGTCTAGAGGAGTTCCAGGAAGAGTTTAAGGATATAAATCAAGAGGAGCAGATCTCCAGGCTTCACAAAATACTTGCTCCTCATCTTCTTAGAAGGGTGAAAAAAGATGTCATGAAAGAACTACCTCCTAAGAAGGAACTTATATTACGCATTGAATTAAGCAGCAAGCAGAAAGAATATTACAAAGCAATTCTGACTCGTAACTATCAGATACTGACTCGTCGTGGTGGTGCACAGATATCTCTTATCAATGTGGTCATGGAATTGCGCAAGCTCTGTTGTCATGCTTATATGCTAGAGGGAGTCGAACCGGACATAGAAGATGCCAATGAATCTCACAAACAACTTTTGGAATCTTCTGGAAAACTGCAAGTATTGGACAAAATGCTGGTGAAACTTAAGCAGCAAGGGCACAGGGTGCTTATCTATTCACAATTTCAACACATGTTGGATTTGTTAGAAGACTACTGTACATACAAAAAATGGCAGTATGAACGAATAGATGGTAAAGTTGGTGGAGCTGAGAGACAAATAAGGATAGATCGATTTAATGCCAAAAATTCTTCCAGGTTTTGCTTTCTGCTTTCCACTAGAGCTGGGGGCTTGGGCATAAACCTTGCAACTGCTGACACAGTGATCATATATGATAGTGATTGGAATCCTCATGCTGATCTACAAGCTATGGCTAGAGCTCATCGACTTGGGCAAACTAACAAGGTGATGATTTATAGGCTAATAACACGAGGAACGATTGAAGAAAGAATGATGCAACTGACTAAGAAGAAAATGGTATTGGAGCATTTGGTTGTTGGAAGGTTAAAGGCTCAAAACATCAACCAGGAAGAGCTCGATGACATCATTAGATATGGATCCAAGGAGTTATTTGCTGATGAAAATGATGAAGTAGGAAAATCCCGTCAAATTCATTATGATGATGCCGCGATAGATAGATTACTTGATCGTGAACAAGCTGGGGATGATGATGCTGTGTTGGAGGACGAAGATGAAGATGGATTTTTAAAGGCCTTTAAGGTTGCAAATTTTGAATACATTGACGAAGCAGAGGCCGTAGAAGAGGAGGAACCAGAAAAGGCTGCAGTGGACAGTAGACCTACAGAAAGCGTTTCAGAGAAGACAAATTTCTGGGAAGATTTGTTAAAGGACAGATATGAAGTGCATAAAGTTGAAGAATTTAATGCTTTAGGCAAAGGGAAGAGAAGTCGTAAGCAGATGGTATCTGTTGAAGAAGATGATCTCGCTGGTTTGGAAGATGTAAGTTCTGATGGAGAGGATGATAACTATGAAGCTGAAGTGACGGATGGCGAAACAGCTTCCTCTGCACCTCTTTCTGGAACTCTTTCTGGGAGAAAGCCTAATAAAAAGAGAAGTCGCGTGGATAGTGCAGAACCCCTTCCTTTGATGGAAGGAGAAGGAAGATCATTCAAAGTCTTGGGTTTCAATCAAAATCAGAGGGCTACATTTGTACAAATTTTGATGAGGTTTGGGATGGGGGATTATGATTGGAAGGAGTTTGTAGCACGCATGAAGAAGACATATGAAGAAATAAAAGCTTATGGTACTCTATTCTTGGAACACATAGTTGAACAACCATTAACAGACTCCCCAACCTTTTCAGATGGTGTCCCTAAAGAAGGACTCAGGATACAAGATGTACTTGTTCGGATTTCTGTTATGTCTATGATTCATCAGAAAGCGAAGTTTGCAACAGAAAATCCAGGCGCTCCTTTGTATGAAGATGACATCATGTTCCGTTACCCAGGACTGAAGGGTAGCAAATATTGGAAGGAGTATCATGATGGAATATTACTGCGTGCCGTATTAAAGCATGGCTATGGAAGATGGCAAGCTATTGTTGATGACAAGGACCTGGGGATTCAAGAAGTTATTTGTCAGGAGTTGAATCTTCCTTTTATGAATCTTCCTGTCCCAGGGCAGGTTAAAGCACAAAATACTGAAGCACCTGGTAACGCTGCTGGAAATGGTAATGGAAATGACATAGGAGAAAATGTAGTTCAGGGAACCATTGATCCAGTAAACCAACCTCAATCATATCAAGAGTCTTCTACGTTGCATCATTTTAGAGATATGCAAAGAAGATTGGTAGAATTTATCAAGCGAAGAGTGCTTCTCTTGGAGAAAGGCAACAATGCAGAAGCCCAGAAAGAATACTTTGATGAATTGTATGCAAATGAGATTGCGGAGCTTGAGAAGGAAACCATGGGGACAAATCTGCCATACACTGGGGAGACTGATAAGCAAATGGGCGATCAGTTGCCAAGAGTTGAAGAAATCGGTTCGGAAGAAATTTTAGCTGCAGCTTGTGACAACGATCCAGATCGTTTAAAATTGCCTCAGCTTTATAATGAGATGTGCAAACTAGTGGAAGGAACTGCCCCTCAGTCACTGCAGACTTCTTTTGAAACTATCTGCGAAGACATACTAAGCAGGACTCGCACCCCTGTGCAACAAAATCCTCCTACCTCAGCACAGCCAACACTAAATGCAGATAGACAACCAGGAGATGAGTCAAAGAGCAATCTTGTAGTCCCTGAATCACCCTCTGCACAAGATAATGCTGCCGAAGTTCAAATGAGAGATTTGGCAACAGAACCCAAGCCCGAAACTGTCCCTGAAACAGCAGATGAATAG

Protein Analysis

1374

Amino Acids

157.52

Weight (kDa)

5.31

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 54 - 95 1.4e-07 PHD-finger
Chromo PF00385 115 - 155 2.2e-08 Chromo (CHRromatin Organisation MOdifier) domain
Chromo PF00385 193 - 240 7.6e-14 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 283 - 464 6.3e-10 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 287 - 583 5.3e-80 SNF2-related domain
HDA2-3 PF11496 536 - 748 3.4e-09 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 607 - 720 1.1e-24 Helicase conserved C-terminal domain
DUF1087 PF06465 843 - 903 4.3e-24 CHD subfamily II, DUF1087
CHDII_SANT-like PF06461 939 - 1071 7.3e-56 CHD subfamily II, SANT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 54, 3837
Acc16I TGCGCA 2 cut(s) 26, 1724
Acc36I ACCTGC 1 cut(s) 3400
AccB1I GGYRCC 1 cut(s) 1060
AccB7I CCANNNNNTGG 1 cut(s) 3716
AccI GTMKAC 1 cut(s) 2563
AccII CGCG 2 cut(s) 2396, 2847
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 3 cut(s) 1109, 2394, 3674
AclWI GGATC 7 cut(s) 583, 596, 1214, 2319, 2332, 3497, 3800
AcoI YGGCCR 1 cut(s) 342
AcuI CTGAAG 4 cut(s) 1379, 2784, 3260, 3450
AcyI GRCGYC 1 cut(s) 357
AdeI CACNNNGTG 1 cut(s) 1873
AfaI GTAC 7 cut(s) 479, 847, 1355, 1927, 2940, 3034, 3129
AfiI CCNNNNNNNGG 6 cut(s) 247, 2804, 3107, 3404, 3409, 3716
AflII CTTAAG 1 cut(s) 1853
AflIII ACRYGT 1 cut(s) 1899
AhlI ACTAGT 1 cut(s) 3853
AjiI CACGTC 1 cut(s) 485
AjuI GAANNNNNNNTTGG 2 cut(s) 2231, 2263
AleI CACNNNNGTG 1 cut(s) 3935
AloI GAACNNNNNNTCC 2 cut(s) 3487, 3519
Alw21I GWGCWC 4 cut(s) 112, 1690, 2149, 2304
Alw26I GTCTC 4 cut(s) 194, 1316, 1975, 3716
Alw44I GTGCAC 1 cut(s) 1686
AlwI GGATC 7 cut(s) 583, 596, 1214, 2319, 2332, 3497, 3800
AlwNI CAGNNNCTG 2 cut(s) 1669, 3880
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 4 cut(s) 342, 1303, 2478, 2520
ApaLI GTGCAC 1 cut(s) 1686
ApeKI GCWGC 9 cut(s) 22, 1442, 1617, 1858, 2549, 3446, 3788, 3791, 4052
Asp700I GAANNNNTTC 2 cut(s) 2812, 2865
AspLEI GCGC 3 cut(s) 27, 1725, 3197
AspS9I GGNCC 2 cut(s) 897, 3340
AsuHPI GGTGA 5 cut(s) 92, 1564, 1858, 2185, 4023
AsuII TTCGAA 1 cut(s) 816
AvaII GGWCC 2 cut(s) 897, 3340
BaeGI GKGCMC 3 cut(s) 1063, 1690, 1869
BamHI GGATCC 2 cut(s) 588, 2324
BanI GGYRCC 1 cut(s) 1060
BanII GRGCYC 3 cut(s) 293, 2149, 2304
BauI CACGAG 1 cut(s) 2196
BbsI GAAGAC 5 cut(s) 1923, 2594, 3011, 3528, 3915
Bbv12I GWGCWC 4 cut(s) 112, 1690, 2149, 2304
BbvCI CCTCAGC 2 cut(s) 3828, 3958
BbvI GCAGC 9 cut(s) 9, 1429, 1629, 1870, 2536, 3433, 3775, 3803, 4039
BceAI ACGGC 2 cut(s) 2507, 3275
BciVI GTATCC 1 cut(s) 3111
BclI TGATCA 1 cut(s) 2091
BcoDI GTCTC 4 cut(s) 194, 1316, 1975, 3716
BcuI ACTAGT 1 cut(s) 3853
BfaI CTAG 7 cut(s) 729, 1004, 1460, 1751, 2046, 2142, 3854
BfmI CTRYAG 4 cut(s) 2550, 2568, 3789, 3881
BfoI RGCGCY 1 cut(s) 3198
BfrI CTTAAG 1 cut(s) 1853
BfuAI ACCTGC 1 cut(s) 3400
BfuI GTATCC 1 cut(s) 3111
BglII AGATCT 2 cut(s) 731, 1506
BmcAI AGTACT 1 cut(s) 1355
Bme18I GGWCC 2 cut(s) 897, 3340
BmgBI CACGTC 1 cut(s) 485
BmgT120I GGNCC 2 cut(s) 897, 3340
BmiI GGNNCC 7 cut(s) 252, 590, 1014, 1062, 2326, 2538, 3496
BmrI ACTGGG 1 cut(s) 3726
BmsI GCATC 9 cut(s) 763, 862, 980, 1429, 1768, 2211, 2380, 2428, 3556
BmuI ACTGGG 1 cut(s) 3726
BpiI GAAGAC 5 cut(s) 1923, 2594, 3011, 3528, 3915
BplI GAGNNNNNCTC 4 cut(s) 1493, 1525, 3588, 3620
BpmI CTGGAG 2 cut(s) 861, 1495
Bpu10I CCTNAGC 3 cut(s) 111, 3828, 3958
Bpu14I TTCGAA 1 cut(s) 816
BpuEI CTTGAG 1 cut(s) 3701
Bsa29I ATCGAT 1 cut(s) 1999
BsaAI YACGTR 1 cut(s) 1118
BsaBI GATNNNNATC 4 cut(s) 1491, 2112, 2323, 3810
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 6 cut(s) 2328, 3232, 3343, 3403, 3404, 3693
BsaWI WCCGGW 2 cut(s) 277, 1765
BsaXI ACNNNNNCTCC 4 cut(s) 1372, 1402, 3468, 3498
Bsc4I CCNNNNNNNGG 6 cut(s) 247, 2804, 3107, 3404, 3409, 3716
Bse1I ACTGG 5 cut(s) 97, 1361, 1387, 3506, 3721
Bse3DI GCAATG 2 cut(s) 430, 1373
Bse8I GATNNNNATC 4 cut(s) 1491, 2112, 2323, 3810
BseAI TCCGGA 1 cut(s) 277
BseCI ATCGAT 1 cut(s) 1999
BseDI CCNNGG 6 cut(s) 2328, 3232, 3343, 3403, 3404, 3693
BseGI GGATG 5 cut(s) 853, 2437, 2752, 2779, 2967
BseJI GATNNNNATC 4 cut(s) 1491, 2112, 2323, 3810
BseLI CCNNNNNNNGG 6 cut(s) 247, 2804, 3107, 3404, 3409, 3716
BseMI GCAATG 2 cut(s) 430, 1373
BseMII CTCAG 8 cut(s) 125, 438, 749, 1968, 3127, 3842, 3887, 3972
BseNI ACTGG 5 cut(s) 97, 1361, 1387, 3506, 3721
BseRI GAGGAG 6 cut(s) 243, 1380, 1478, 1514, 1524, 2546
BseSI GKGCMC 3 cut(s) 1063, 1690, 1869
BseXI GCAGC 9 cut(s) 9, 1429, 1629, 1870, 2536, 3433, 3775, 3803, 4039
BseYI CCCAGC 3 cut(s) 367, 2051, 2426
BsgI GTGCAG 3 cut(s) 2778, 2877, 4023
Bsh1236I CGCG 2 cut(s) 2396, 2847
BshFI GGCC 4 cut(s) 344, 1305, 2480, 2522
BshNI GGYRCC 1 cut(s) 1060
BshVI ATCGAT 1 cut(s) 1999
BsiHKAI GWGCWC 4 cut(s) 112, 1690, 2149, 2304
BsiSI CCGG 2 cut(s) 278, 1766
BslFI GGGAC 8 cut(s) 103, 948, 1295, 3083, 3386, 3712, 4007, 4088
BslI CCNNNNNNNGG 6 cut(s) 247, 2804, 3107, 3404, 3409, 3716
BsmAI GTCTC 4 cut(s) 194, 1316, 1975, 3716
BsmBI CGTCTC 1 cut(s) 1316
BsmFI GGGAC 8 cut(s) 103, 948, 1295, 3083, 3386, 3712, 4007, 4088
BsmI GAATGC 1 cut(s) 662
BsnI GGCC 4 cut(s) 344, 1305, 2480, 2522
Bsp119I TTCGAA 1 cut(s) 816
Bsp1286I GDGCHC 7 cut(s) 112, 293, 1063, 1690, 1869, 2149, 2304
Bsp13I TCCGGA 1 cut(s) 277
Bsp1407I TGTACA 2 cut(s) 1925, 2938
Bsp19I CCATGG 1 cut(s) 3693
BspACI CCGC 3 cut(s) 1109, 2394, 3674
BspANI GGCC 4 cut(s) 344, 1305, 2480, 2522
BspCNI CTCAG 8 cut(s) 124, 439, 748, 1969, 3126, 3841, 3886, 3971
BspDI ATCGAT 1 cut(s) 1999
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 2 cut(s) 2396, 2847
BspHI TCATGA 2 cut(s) 1566, 3268
BspLI GGNNCC 7 cut(s) 252, 590, 1014, 1062, 2326, 2538, 3496
BspMAI CTGCAG 3 cut(s) 2554, 3793, 3885
BspMI ACCTGC 1 cut(s) 3400
BspPI GGATC 7 cut(s) 583, 596, 1214, 2319, 2332, 3497, 3800
BspQI GCTCTTC 2 cut(s) 1099, 2292
BspT104I TTCGAA 1 cut(s) 816
BspT107I GGYRCC 1 cut(s) 1060
BspTI CTTAAG 1 cut(s) 1853
BsrDI GCAATG 2 cut(s) 430, 1373
BsrGI TGTACA 2 cut(s) 1925, 2938
BsrI ACTGG 5 cut(s) 97, 1361, 1387, 3506, 3721
BssECI CCNNGG 6 cut(s) 2328, 3232, 3343, 3403, 3404, 3693
BssNI GRCGYC 1 cut(s) 357
BssSI CACGAG 1 cut(s) 2196
BssT1I CCWWGG 2 cut(s) 2328, 3693
Bst2BI CACGAG 1 cut(s) 2196
Bst6I CTCTTC 9 cut(s) 752, 978, 1099, 1398, 1470, 2292, 2523, 2663, 3589
BstACI GRCGYC 1 cut(s) 357
BstAFI CTTAAG 1 cut(s) 1853
BstAPI GCANNNNNTGC 2 cut(s) 221, 1873
BstAUI TGTACA 2 cut(s) 1925, 2938
BstBAI YACGTR 1 cut(s) 1118
BstBI TTCGAA 1 cut(s) 816
BstC8I GCNNGC 8 cut(s) 269, 598, 1196, 1622, 1727, 2995, 3288, 3321
BstDSI CCRYGG 1 cut(s) 3693
BstENI CCTNNNNNAGG 2 cut(s) 3105, 3402
BstF5I GGATG 5 cut(s) 853, 2437, 2752, 2779, 2967
BstFNI CGCG 2 cut(s) 2396, 2847
BstH2I RGCGCY 1 cut(s) 3198
BstHHI GCGC 3 cut(s) 27, 1725, 3197
BstMAI GTCTC 4 cut(s) 194, 1316, 1975, 3716
BstNSI RCATGY 2 cut(s) 207, 1903
BstSFI CTRYAG 4 cut(s) 2550, 2568, 3789, 3881
BstSLI GKGCMC 3 cut(s) 1063, 1690, 1869
BstUI CGCG 2 cut(s) 2396, 2847
BstV1I GCAGC 9 cut(s) 9, 1429, 1629, 1870, 2536, 3433, 3775, 3803, 4039
BstV2I GAAGAC 5 cut(s) 1923, 2594, 3011, 3528, 3915
BstX2I RGATCY 4 cut(s) 588, 731, 1506, 2324
BstYI RGATCY 4 cut(s) 588, 731, 1506, 2324
Bsu15I ATCGAT 1 cut(s) 1999
BsuI GTATCC 1 cut(s) 3111
BsuRI GGCC 4 cut(s) 344, 1305, 2480, 2522
BsuTUI ATCGAT 1 cut(s) 1999
BtgI CCRYGG 1 cut(s) 3693
BtgZI GCGATG 2 cut(s) 286, 1352
BtrI CACGTC 1 cut(s) 485
BtsCI GGATG 5 cut(s) 853, 2437, 2752, 2779, 2967
BtsI GCAGTG 3 cut(s) 848, 2559, 3878
BtsIMutI CAGTG 7 cut(s) 848, 1189, 1262, 2094, 2559, 3714, 3878
BveI ACCTGC 1 cut(s) 3400
Cac8I GCNNGC 8 cut(s) 269, 598, 1196, 1622, 1727, 2995, 3288, 3321
CaiI CAGNNNCTG 2 cut(s) 1669, 3880
CciI TCATGA 2 cut(s) 1566, 3268
CfoI GCGC 3 cut(s) 27, 1725, 3197
Cfr13I GGNCC 2 cut(s) 897, 3340
ClaI ATCGAT 1 cut(s) 1999
CseI GACGC 1 cut(s) 365
CsiI ACCWGGT 1 cut(s) 3436
Csp6I GTAC 7 cut(s) 478, 846, 1354, 1926, 2939, 3033, 3128
CviQI GTAC 7 cut(s) 478, 846, 1354, 1926, 2939, 3033, 3128
DraI TTTAAA 2 cut(s) 2475, 3819
DraIII CACNNNGTG 1 cut(s) 1873
EaeI YGGCCR 1 cut(s) 342
Eam1104I CTCTTC 9 cut(s) 752, 978, 1099, 1398, 1470, 2292, 2523, 2663, 3589
EarI CTCTTC 9 cut(s) 752, 978, 1099, 1398, 1470, 2292, 2523, 2663, 3589
Ecl136II GAGCTC 2 cut(s) 2147, 2302
Eco130I CCWWGG 2 cut(s) 2328, 3693
Eco147I AGGCCT 1 cut(s) 2480
Eco24I GRGCYC 3 cut(s) 293, 2149, 2304
Eco32I GATATC 2 cut(s) 682, 1695
Eco47I GGWCC 2 cut(s) 897, 3340
Eco53kI GAGCTC 2 cut(s) 2147, 2302
Eco57I CTGAAG 4 cut(s) 1379, 2784, 3260, 3450
EcoICRI GAGCTC 2 cut(s) 2147, 2302
EcoNI CCTNNNNNAGG 2 cut(s) 3105, 3402
EcoO109I RGGNCCY 1 cut(s) 3340
EcoRV GATATC 2 cut(s) 682, 1695
EcoT14I CCWWGG 2 cut(s) 2328, 3693
EcoT22I ATGCAT 1 cut(s) 217
EcoT38I GRGCYC 3 cut(s) 293, 2149, 2304
ErhI CCWWGG 2 cut(s) 2328, 3693
Esp3I CGTCTC 1 cut(s) 1316
FalI AAGNNNNNCTT 2 cut(s) 3114, 3146
FaqI GGGAC 8 cut(s) 103, 948, 1295, 3083, 3386, 3712, 4007, 4088
FauNDI CATATG 2 cut(s) 1234, 3010
FbaI TGATCA 1 cut(s) 2091
FblI GTMKAC 1 cut(s) 2563
FokI GGATG 5 cut(s) 840, 2444, 2759, 2786, 2974
FriOI GRGCYC 3 cut(s) 293, 2149, 2304
FspBI CTAG 7 cut(s) 729, 1004, 1460, 1751, 2046, 2142, 3854
FspI TGCGCA 2 cut(s) 26, 1724
GlaI GCGC 3 cut(s) 26, 1724, 3196
GsaI CCCAGC 3 cut(s) 371, 2055, 2430
GsuI CTGGAG 2 cut(s) 861, 1495
HaeII RGCGCY 1 cut(s) 3198
HaeIII GGCC 4 cut(s) 344, 1305, 2480, 2522
HapII CCGG 2 cut(s) 278, 1766
HgaI GACGC 1 cut(s) 365
HhaI GCGC 3 cut(s) 27, 1725, 3197
Hin1I GRCGYC 1 cut(s) 357
Hin6I GCGC 3 cut(s) 25, 1723, 3195
HinP1I GCGC 3 cut(s) 25, 1723, 3195
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 1 cut(s) 3024
HpaI GTTAAC 1 cut(s) 502
HpaII CCGG 2 cut(s) 278, 1766
HphI GGTGA 5 cut(s) 92, 1564, 1858, 2185, 4023
Hpy166II GTNNAC 8 cut(s) 233, 502, 848, 1688, 2420, 2557, 2564, 3511
Hpy8I GTNNAC 8 cut(s) 233, 502, 848, 1688, 2420, 2557, 2564, 3511
Hpy99I CGWCG 2 cut(s) 611, 1680
HpyCH4IV ACGT 5 cut(s) 476, 484, 886, 1117, 3542
HpySE526I ACGT 5 cut(s) 476, 484, 886, 1117, 3542
Hsp92I GRCGYC 1 cut(s) 357
HspAI GCGC 3 cut(s) 25, 1723, 3195
Kpn2I TCCGGA 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 2091
KspAI GTTAAC 1 cut(s) 502
LguI GCTCTTC 2 cut(s) 1099, 2292
LmnI GCTCC 8 cut(s) 256, 1018, 1501, 1537, 1973, 2251, 3202, 3676
Lsp1109I GCAGC 9 cut(s) 9, 1429, 1629, 1870, 2536, 3433, 3775, 3803, 4039
LweI GCATC 9 cut(s) 763, 862, 980, 1429, 1768, 2211, 2380, 2428, 3556
MabI ACCWGGT 1 cut(s) 3436
MaeI CTAG 7 cut(s) 729, 1004, 1460, 1751, 2046, 2142, 3854
MaeII ACGT 5 cut(s) 476, 484, 886, 1117, 3542
MaeIII GTNAC 7 cut(s) 353, 1655, 2767, 3227, 3440, 3797, 3876
MflI RGATCY 4 cut(s) 588, 731, 1506, 2324
MhlI GDGCHC 7 cut(s) 112, 293, 1063, 1690, 1869, 2149, 2304
MmeI TCCRAC 3 cut(s) 1884, 2245, 2427
Mph1103I ATGCAT 1 cut(s) 217
MroI TCCGGA 1 cut(s) 277
MroXI GAANNNNTTC 2 cut(s) 2812, 2865
MslI CAYNNNNRTG 4 cut(s) 1424, 2385, 3306, 3935
MspA1I CMGCKG 1 cut(s) 267
MspCI CTTAAG 1 cut(s) 1853
MspI CCGG 2 cut(s) 278, 1766
Mva1269I GAATGC 1 cut(s) 662
MvnI CGCG 2 cut(s) 2396, 2847
NcoI CCATGG 1 cut(s) 3693
NdeI CATATG 2 cut(s) 1234, 3010
NlaIV GGNNCC 7 cut(s) 252, 590, 1014, 1062, 2326, 2538, 3496
NmuCI GTSAC 4 cut(s) 353, 2767, 3797, 3876
NsbI TGCGCA 2 cut(s) 26, 1724
NsiI ATGCAT 1 cut(s) 217
NspI RCATGY 2 cut(s) 207, 1903
NspV TTCGAA 1 cut(s) 816
OliI CACNNNNGTG 1 cut(s) 3935
PagI TCATGA 2 cut(s) 1566, 3268
PasI CCCWGGG 1 cut(s) 3404
PceI AGGCCT 1 cut(s) 2480
PciI ACATGT 1 cut(s) 1899
PciSI GCTCTTC 2 cut(s) 1099, 2292
PcsI WCGNNNNNNNCGW 2 cut(s) 37, 69
PctI GAATGC 1 cut(s) 662
PdmI GAANNNNTTC 2 cut(s) 2812, 2865
PflMI CCANNNNNTGG 1 cut(s) 3716
PfoI TCCNGGA 1 cut(s) 1470
Ppu21I YACGTR 1 cut(s) 1118
PpuMI RGGWCCY 1 cut(s) 3340
PscI ACATGT 1 cut(s) 1899
PsiI TTATAA 2 cut(s) 54, 3837
Psp124BI GAGCTC 2 cut(s) 2149, 2304
Psp5II RGGWCCY 1 cut(s) 3340
PspFI CCCAGC 3 cut(s) 367, 2051, 2426
PspN4I GGNNCC 7 cut(s) 252, 590, 1014, 1062, 2326, 2538, 3496
PspPI GGNCC 2 cut(s) 897, 3340
PspPPI RGGWCCY 1 cut(s) 3340
PsrI GAACNNNNNNTAC 2 cut(s) 1584, 1616
PstI CTGCAG 3 cut(s) 2554, 3793, 3885
PstNI CAGNNNCTG 2 cut(s) 1669, 3880
PsuI RGATCY 4 cut(s) 588, 731, 1506, 2324
PvuII CAGCTG 1 cut(s) 267
RsaI GTAC 7 cut(s) 479, 847, 1355, 1927, 2940, 3034, 3129
RsaNI GTAC 7 cut(s) 478, 846, 1354, 1926, 2939, 3033, 3128
RseI CAYNNNNRTG 4 cut(s) 1424, 2385, 3306, 3935
SacI GAGCTC 2 cut(s) 2149, 2304
SapI GCTCTTC 2 cut(s) 1099, 2292
Sau96I GGNCC 2 cut(s) 897, 3340
ScaI AGTACT 1 cut(s) 1355
SduI GDGCHC 7 cut(s) 112, 293, 1063, 1690, 1869, 2149, 2304
SexAI ACCWGGT 1 cut(s) 3436
SfaNI GCATC 9 cut(s) 763, 862, 980, 1429, 1768, 2211, 2380, 2428, 3556
SfcI CTRYAG 4 cut(s) 2550, 2568, 3789, 3881
SfuI TTCGAA 1 cut(s) 816
SinI GGWCC 2 cut(s) 897, 3340
SmiMI CAYNNNNRTG 4 cut(s) 1424, 2385, 3306, 3935
SmlI CTYRAG 2 cut(s) 1853, 3680
SmoI CTYRAG 2 cut(s) 1853, 3680
SpeI ACTAGT 1 cut(s) 3853
SseBI AGGCCT 1 cut(s) 2480
SsiI CCGC 3 cut(s) 1109, 2394, 3674
SspI AATATT 4 cut(s) 58, 1634, 3254, 3279
SspMI CTAG 7 cut(s) 729, 1004, 1460, 1751, 2046, 2142, 3854
SstI GAGCTC 2 cut(s) 2149, 2304
StuI AGGCCT 1 cut(s) 2480
StyI CCWWGG 2 cut(s) 2328, 3693
TaiI ACGT 5 cut(s) 479, 487, 889, 1120, 3545
TaqII GACCGA 1 cut(s) 54
TatI WGTACW 4 cut(s) 1353, 1925, 2938, 3127
TauI GCSGC 1 cut(s) 2396
TscAI CASTG 7 cut(s) 855, 1189, 1269, 2094, 2559, 3721, 3885
TseFI GTSAC 4 cut(s) 353, 2767, 3797, 3876
TseI GCWGC 9 cut(s) 22, 1442, 1617, 1858, 2549, 3446, 3788, 3791, 4052
Tsp45I GTSAC 4 cut(s) 353, 2767, 3797, 3876
TspGWI ACGGA 2 cut(s) 2786, 3215
TspRI CASTG 7 cut(s) 855, 1189, 1269, 2094, 2559, 3721, 3885
Van91I CCANNNNNTGG 1 cut(s) 3716
Vha464I CTTAAG 1 cut(s) 1853
VneI GTGCAC 1 cut(s) 1686
VpaK11BI GGWCC 2 cut(s) 897, 3340
XagI CCTNNNNNAGG 2 cut(s) 3105, 3402
XbaI TCTAGA 2 cut(s) 728, 1459
XceI RCATGY 2 cut(s) 207, 1903
XmiI GTMKAC 1 cut(s) 2563
XmnI GAANNNNTTC 2 cut(s) 2812, 2865
XspI CTAG 7 cut(s) 729, 1004, 1460, 1751, 2046, 2142, 3854
ZrmI AGTACT 1 cut(s) 1355
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.