Rh7DG001900

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007421
Physical Location & Seq
Forward (+)
216154 .. 218810
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG001900.1

Sequence Viewer

Length: 651 bp
ATGTCATTGCCTTCCGTACAACTGAAACAACCCAATAATGTCATCACCTGCAAAGGCTTGGGTGCTGCTTGGAATGTTGCTGATATATCTAATGGATCAAGTGTGGTGATATTTGGTCTCGGAACCGTAGGCCTCTCTGTTGCACAGGGTGCTAAGCTTAGGGGCGCATCAGCAATAATTGGTGTTGACACTAATCCTGAAAAGGAAGAAAAAGCAAAAGCTTTTGGAATTACCGAATTTATTAACCCCAATGACAGTAAAGAACCTGTTCAACAGGTTATTAAGCATCTCACAGGTGGAGGGGCAGATTACGCATTTGAATGTATAGGTGACACAGGAATGGTAACTACTGCATTGCAGGCATGTTGCGATGGATGGGGATTGACTGTGACGCTTGGTGTGCCAAAAGAAAAACCAGAAGTATCTGCTCATTATGGAATATTTCTAACTGGAAGAACATTGAAAGGGTCTCTTTTTGGAGGATGGAGACCTAAATCGGATCTCCCTTCGTTAGTTGACATGTACATAAAAAAGGAAATCCAAGTTGATGAGTACATAACACATAACCTGTCATTTAAAGATATCAACAAAGCTTTTGATCTGATGAGAGAAGGGAAGTGTTTGCGTTGTGTTATGCACATGGAAAAGTAG

Protein Analysis

216

Amino Acids

23.25

Weight (kDa)

6.89

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 43 - 174 3.7e-26 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 56
Acc36I ACCTGC 1 cut(s) 56
AclWI GGATC 2 cut(s) 103, 507
AcsI RAATTY 1 cut(s) 236
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 3 cut(s) 18, 524, 554
AflIII ACRYGT 1 cut(s) 519
AgsI TTSAA 3 cut(s) 272, 320, 463
AluBI AGCT 3 cut(s) 157, 221, 593
AluI AGCT 3 cut(s) 157, 221, 593
Alw26I GTCTC 3 cut(s) 122, 474, 481
AlwI GGATC 2 cut(s) 103, 507
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 65
ApoI RAATTY 1 cut(s) 236
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 1 cut(s) 167
AsuHPI GGTGA 3 cut(s) 37, 118, 341
BbvI GCAGC 1 cut(s) 52
BccI CCATC 3 cut(s) 365, 369, 477
BcoDI GTCTC 3 cut(s) 122, 474, 481
BfuAI ACCTGC 1 cut(s) 56
BisI GCNGC 1 cut(s) 66
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 124
BmsI GCATC 2 cut(s) 176, 295
Bpu10I CCTNAGC 1 cut(s) 158
Bpu1102I GCTNAGC 1 cut(s) 153
BsaI GGTCTC 3 cut(s) 122, 474, 481
Bse1I ACTGG 1 cut(s) 454
Bse3DI GCAATG 2 cut(s) 5, 353
BseGI GGATG 2 cut(s) 380, 488
BseMI GCAATG 2 cut(s) 5, 353
BseNI ACTGG 1 cut(s) 454
BseXI GCAGC 1 cut(s) 52
BshFI GGCC 1 cut(s) 132
BsmAI GTCTC 3 cut(s) 122, 474, 481
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 3 cut(s) 122, 474, 481
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 3 cut(s) 95, 499, 598
Bsp1720I GCTNAGC 1 cut(s) 153
BspANI GGCC 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 2 cut(s) 103, 507
BspTNI GGTCTC 3 cut(s) 122, 474, 481
BsrDI GCAATG 2 cut(s) 5, 353
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 1 cut(s) 454
BssMI GATC 3 cut(s) 95, 499, 598
Bst4CI ACNGT 3 cut(s) 127, 257, 388
BstAPI GCANNNNNTGC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 2 cut(s) 153, 158
BstF5I GGATG 2 cut(s) 380, 488
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 3 cut(s) 98, 502, 601
BstMAI GTCTC 3 cut(s) 122, 474, 481
BstMBI GATC 3 cut(s) 95, 499, 598
BstMWI GCNNNNNNNGC 4 cut(s) 149, 311, 359, 400
BstNSI RCATGY 2 cut(s) 366, 523
BstV1I GCAGC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 380, 488
BveI ACCTGC 1 cut(s) 56
Cac8I GCNNGC 1 cut(s) 360
CfoI GCGC 1 cut(s) 167
CseI GACGC 1 cut(s) 400
Csp6I GTAC 3 cut(s) 17, 523, 553
CviAII CATG 3 cut(s) 363, 520, 640
CviJI RGCY 5 cut(s) 57, 132, 157, 221, 593
CviKI_1 RGCY 5 cut(s) 57, 132, 157, 221, 593
CviQI GTAC 3 cut(s) 17, 523, 553
DdeI CTNAG 2 cut(s) 153, 158
DpnI GATC 3 cut(s) 97, 501, 600
DpnII GATC 3 cut(s) 95, 499, 598
DraI TTTAAA 1 cut(s) 577
DraIII CACNNNGTG 1 cut(s) 149
Eco147I AGGCCT 1 cut(s) 132
Eco31I GGTCTC 3 cut(s) 122, 474, 481
Eco32I GATATC 1 cut(s) 583
EcoRV GATATC 1 cut(s) 583
FaeI CATG 3 cut(s) 366, 523, 643
FalI AAGNNNNNCTT 2 cut(s) 456, 488
FatI CATG 3 cut(s) 362, 519, 639
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 2 cut(s) 387, 495
Fsp4HI GCNGC 1 cut(s) 66
GlaI GCGC 1 cut(s) 166
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 132
HgaI GACGC 1 cut(s) 400
HhaI GCGC 1 cut(s) 167
Hin1II CATG 3 cut(s) 366, 523, 643
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 2 cut(s) 187, 517
HindII GTYRAC 2 cut(s) 187, 517
HindIII AAGCTT 3 cut(s) 155, 219, 591
HphI GGTGA 3 cut(s) 37, 118, 341
Hpy166II GTNNAC 2 cut(s) 187, 517
Hpy188I TCNGA 3 cut(s) 122, 499, 603
Hpy188III TCNNGA 1 cut(s) 197
Hpy8I GTNNAC 2 cut(s) 187, 517
HpyAV CCTTC 3 cut(s) 21, 516, 605
HpyCH4III ACNGT 3 cut(s) 127, 257, 388
HpyCH4V TGCA 5 cut(s) 51, 143, 353, 358, 637
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 311, 359, 400
HpyF3I CTNAG 2 cut(s) 153, 158
Hsp92II CATG 3 cut(s) 366, 523, 643
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 3 cut(s) 95, 499, 598
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 2 cut(s) 176, 295
MaeIII GTNAC 3 cut(s) 329, 343, 388
MalI GATC 3 cut(s) 97, 501, 600
MboI GATC 3 cut(s) 95, 499, 598
MboII GAAGA 2 cut(s) 218, 465
MflI RGATCY 1 cut(s) 499
MluCI AATT 3 cut(s) 177, 228, 236
MnlI CCTC 3 cut(s) 143, 293, 473
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 3 cut(s) 243, 282, 576
MslI CAYNNNNRTG 2 cut(s) 319, 338
MwoI GCNNNNNNNGC 4 cut(s) 149, 311, 359, 400
NdeII GATC 3 cut(s) 95, 499, 598
NlaIII CATG 3 cut(s) 366, 523, 643
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 2 cut(s) 329, 388
NspI RCATGY 2 cut(s) 366, 523
PaqCI CACCTGC 1 cut(s) 56
PceI AGGCCT 1 cut(s) 132
PciI ACATGT 1 cut(s) 519
PdmI GAANNNNTTC 1 cut(s) 267
PkrI GCNGC 1 cut(s) 67
PscI ACATGT 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 124
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 3 cut(s) 18, 524, 554
RsaNI GTAC 3 cut(s) 17, 523, 553
RseI CAYNNNNRTG 2 cut(s) 319, 338
SaqAI TTAA 3 cut(s) 243, 282, 576
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 3 cut(s) 95, 499, 598
SfaNI GCATC 2 cut(s) 176, 295
SmiMI CAYNNNNRTG 2 cut(s) 319, 338
Sse9I AATT 3 cut(s) 177, 228, 236
SseBI AGGCCT 1 cut(s) 132
SspI AATATT 1 cut(s) 441
StuI AGGCCT 1 cut(s) 132
TaaI ACNGT 3 cut(s) 127, 257, 388
TasI AATT 3 cut(s) 177, 228, 236
TatI WGTACW 2 cut(s) 522, 552
Tru1I TTAA 3 cut(s) 243, 282, 576
Tru9I TTAA 3 cut(s) 243, 282, 576
TseFI GTSAC 2 cut(s) 329, 388
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 2 cut(s) 329, 388
TspGWI ACGGA 1 cut(s) 4
XapI RAATTY 1 cut(s) 236
XceI RCATGY 2 cut(s) 366, 523
XmnI GAANNNNTTC 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.