Rh1AG048600

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0011610
Physical Location & Seq
Forward (+)
8297140 .. 8297768
629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG048600.1

Sequence Viewer

Length: 429 bp
ATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAACAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATTGTTGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCTGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTTTGCTGCCAAGATGGGTAAGCATTCTTTGATTGATATCTGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

142

Amino Acids

15.8

Weight (kDa)

5.71

Isoelectric Point (pI)

54.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 1 - 84 2e-32 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 338
AclWI GGATC 2 cut(s) 290, 349
AcuI CTGAAG 2 cut(s) 180, 198
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 1 cut(s) 74
AluBI AGCT 7 cut(s) 88, 158, 197, 227, 257, 335, 373
AluI AGCT 7 cut(s) 88, 158, 197, 227, 257, 335, 373
Alw21I GWGCWC 1 cut(s) 28
AlwI GGATC 2 cut(s) 290, 349
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 3 cut(s) 85, 190, 386
AspA2I CCTAGG 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 46, 232
AsuHPI GGTGA 2 cut(s) 26, 230
AvaII GGWCC 1 cut(s) 46
AvrII CCTAGG 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 97, 202, 373
BccI CCATC 2 cut(s) 58, 388
BceAI ACGGC 1 cut(s) 167
BciVI GTATCC 1 cut(s) 150
BfaI CTAG 2 cut(s) 20, 153
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 3 cut(s) 86, 191, 387
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 3 cut(s) 87, 192, 388
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 46, 232
BmiI GGNNCC 1 cut(s) 48
BmsI GCATC 2 cut(s) 190, 198
BsaBI GATNNNNATC 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 98, 235
Bse8I GATNNNNATC 1 cut(s) 416
BseDI CCNNGG 1 cut(s) 19
BseJI GATNNNNATC 1 cut(s) 416
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 2 cut(s) 41, 277
BseNI ACTGG 2 cut(s) 98, 235
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 3 cut(s) 97, 202, 373
BseYI CCCAGC 1 cut(s) 331
BshFI GGCC 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 28
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 28
Bsp143I GATC 3 cut(s) 132, 282, 341
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 2 cut(s) 40, 276
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 2 cut(s) 290, 349
BsrI ACTGG 2 cut(s) 98, 235
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 3 cut(s) 132, 282, 341
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 297
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 2 cut(s) 27, 263
BstEII GGTNACC 1 cut(s) 14
BstKTI GATC 3 cut(s) 135, 285, 344
BstMBI GATC 3 cut(s) 132, 282, 341
BstMWI GCNNNNNNNGC 2 cut(s) 94, 164
BstPI GGTNACC 1 cut(s) 14
BstV1I GCAGC 3 cut(s) 97, 202, 373
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 195
CciI TCATGA 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 46, 232
CviAII CATG 1 cut(s) 314
DdeI CTNAG 2 cut(s) 27, 263
DpnI GATC 3 cut(s) 134, 284, 343
DpnII GATC 3 cut(s) 132, 282, 341
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 19
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 180, 198
Eco91I GGTNACC 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 46
EcoO65I GGTNACC 1 cut(s) 14
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 1 cut(s) 317
FaiI YATR 6 cut(s) 79, 131, 230, 315, 323, 362
FaqI GGGAC 1 cut(s) 32
FatI CATG 1 cut(s) 313
Fnu4HI GCNGC 3 cut(s) 86, 191, 387
Fsp4HI GCNGC 3 cut(s) 86, 191, 387
FspBI CTAG 2 cut(s) 20, 153
GluI GCNGC 3 cut(s) 86, 191, 387
GsaI CCCAGC 1 cut(s) 335
HaeIII GGCC 1 cut(s) 234
Hin1II CATG 1 cut(s) 317
HinfI GANTC 2 cut(s) 125, 221
HphI GGTGA 2 cut(s) 26, 230
Hpy188I TCNGA 6 cut(s) 30, 178, 207, 266, 355, 367
Hpy188III TCNNGA 2 cut(s) 286, 314
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4IV ACGT 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 164
HpyF3I CTNAG 2 cut(s) 27, 263
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 1 cut(s) 317
Kzo9I GATC 3 cut(s) 132, 282, 341
LmnI GCTCC 1 cut(s) 370
LpnPI CCDG 6 cut(s) 29, 111, 201, 248, 255, 345
Lsp1109I GCAGC 3 cut(s) 97, 202, 373
LweI GCATC 2 cut(s) 190, 198
MaeI CTAG 2 cut(s) 20, 153
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 2 cut(s) 14, 324
MalI GATC 3 cut(s) 134, 284, 343
MboI GATC 3 cut(s) 132, 282, 341
MboII GAAGA 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 28
MlyI GAGTC 1 cut(s) 230
MnlI CCTC 6 cut(s) 5, 180, 273, 282, 291, 361
Mva1269I GAATGC 1 cut(s) 403
MwoI GCNNNNNNNGC 2 cut(s) 94, 164
NdeII GATC 3 cut(s) 132, 282, 341
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 14
PagI TCATGA 1 cut(s) 313
PctI GAATGC 1 cut(s) 403
PfeI GAWTC 1 cut(s) 125
PflMI CCANNNNNTGG 1 cut(s) 338
PkrI GCNGC 3 cut(s) 87, 192, 388
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 46
Psp5II RGGWCCY 1 cut(s) 46
PspEI GGTNACC 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 2 cut(s) 46, 232
PspPPI RGGWCCY 1 cut(s) 46
PsrI GAACNNNNNNTAC 2 cut(s) 309, 341
SatI GCNGC 3 cut(s) 86, 191, 387
Sau3AI GATC 3 cut(s) 132, 282, 341
Sau96I GGNCC 2 cut(s) 46, 232
SchI GAGTC 1 cut(s) 230
SduI GDGCHC 1 cut(s) 28
SfaNI GCATC 2 cut(s) 190, 198
SinI GGWCC 1 cut(s) 46
SspMI CTAG 2 cut(s) 20, 153
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 359
TaiI ACGT 1 cut(s) 313
TaqI TCGA 3 cut(s) 123, 135, 285
TfiI GAWTC 1 cut(s) 125
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 3 cut(s) 85, 190, 386
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 330
Van91I CCANNNNNTGG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 46
XmaJI CCTAGG 1 cut(s) 19
XspI CTAG 2 cut(s) 20, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.