Rh5DG196000

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011610
Physical Location & Seq
Forward (+)
21737187 .. 21747499
10313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG196000.1

Sequence Viewer

Length: 393 bp
ATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGGTAGTCATTCTCCTCGAATGGCTGAAGTTGCCGTTCCTGTAGCACTGGCCTTAATTGACAAGGTGATAAGCATCGTCCAGCAAAACTTTCAACGTCAAACTAGAGTCAAGGATGATGTTGAAAAAGCCCAGAATTATTTGAGGAGGATGGAAGCGTATCTAGCCGACTTATCAGAAGTTCAGAACATATCGATGAGGGTACTCAAGTGTTTCAGACTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

130

Amino Acids

14.92

Weight (kDa)

8.66

Isoelectric Point (pI)

58.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 73 - 123 8.6e-07 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 110
AclWI GGATC 2 cut(s) 62, 121
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 1 cut(s) 369
AfiI CCNNNNNNNGG 1 cut(s) 110
AgsI TTSAA 2 cut(s) 260, 290
AluBI AGCT 3 cut(s) 29, 107, 145
AluI AGCT 3 cut(s) 29, 107, 145
AlwI GGATC 2 cut(s) 62, 121
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 2 cut(s) 4, 216
ApeKI GCWGC 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 386
BaeI ACNNNNGTAYC 2 cut(s) 359, 392
BbvI GCAGC 1 cut(s) 145
BccI CCATC 2 cut(s) 160, 310
BceAI ACGGC 1 cut(s) 185
BfaI CTAG 2 cut(s) 270, 329
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 4
BmsI GCATC 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 356
Bsa29I ATCGAT 1 cut(s) 359
BsaBI GATNNNNATC 1 cut(s) 239
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 7, 219
Bse8I GATNNNNATC 1 cut(s) 239
BseCI ATCGAT 1 cut(s) 359
BseGI GGATG 2 cut(s) 286, 321
BseJI GATNNNNATC 1 cut(s) 239
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMII CTCAG 1 cut(s) 49
BseNI ACTGG 2 cut(s) 7, 219
BseRI GAGGAG 3 cut(s) 155, 171, 325
BseXI GCAGC 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 103
BshFI GGCC 2 cut(s) 6, 218
BshVI ATCGAT 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 386
BslI CCNNNNNNNGG 1 cut(s) 110
BsnI GGCC 2 cut(s) 6, 218
BsoBI CYCGRG 1 cut(s) 386
Bsp143I GATC 2 cut(s) 54, 113
BspANI GGCC 2 cut(s) 6, 218
BspCNI CTCAG 1 cut(s) 48
BspDI ATCGAT 1 cut(s) 359
BspHI TCATGA 1 cut(s) 85
BspPI GGATC 2 cut(s) 62, 121
BsrI ACTGG 2 cut(s) 7, 219
BssMI GATC 2 cut(s) 54, 113
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 35
BstF5I GGATG 2 cut(s) 286, 321
BstKTI GATC 2 cut(s) 57, 116
BstMBI GATC 2 cut(s) 54, 113
BstMWI GCNNNNNNNGC 2 cut(s) 197, 329
BstSFI CTRYAG 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 145
Bsu15I ATCGAT 1 cut(s) 359
BsuRI GGCC 2 cut(s) 6, 218
BsuTUI ATCGAT 1 cut(s) 359
BtsCI GGATG 2 cut(s) 286, 321
BtsIMutI CAGTG 1 cut(s) 212
CciI TCATGA 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 359
Csp6I GTAC 1 cut(s) 368
CviAII CATG 1 cut(s) 86
CviJI RGCY 8 cut(s) 6, 29, 107, 145, 191, 218, 296, 332
CviKI_1 RGCY 8 cut(s) 6, 29, 107, 145, 191, 218, 296, 332
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 1 cut(s) 35
DpnI GATC 2 cut(s) 56, 115
DpnII GATC 2 cut(s) 54, 113
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 386
FaeI CATG 1 cut(s) 89
FaiI YATR 4 cut(s) 87, 95, 134, 356
FatI CATG 1 cut(s) 85
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 2 cut(s) 293, 328
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 2 cut(s) 270, 329
GluI GCNGC 1 cut(s) 159
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 2 cut(s) 6, 218
Hin1II CATG 1 cut(s) 89
HinfI GANTC 2 cut(s) 273, 384
HphI GGTGA 1 cut(s) 244
Hpy188I TCNGA 6 cut(s) 38, 127, 139, 343, 351, 383
Hpy188III TCNNGA 2 cut(s) 58, 86
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 2 cut(s) 82, 262
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 329
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 2 cut(s) 82, 262
Hsp92II CATG 1 cut(s) 89
Kzo9I GATC 2 cut(s) 54, 113
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 7 cut(s) 20, 27, 117, 200, 219, 260, 311
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 1 cut(s) 249
MaeI CTAG 2 cut(s) 270, 329
MaeII ACGT 2 cut(s) 82, 262
MaeIII GTNAC 1 cut(s) 96
MalI GATC 2 cut(s) 56, 115
MboI GATC 2 cut(s) 54, 113
MboII GAAGA 1 cut(s) 86
MluCI AATT 2 cut(s) 222, 301
MlyI GAGTC 2 cut(s) 282, 378
MnlI CCTC 8 cut(s) 45, 54, 63, 133, 192, 303, 306, 357
MseI TTAA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 359
MwoI GCNNNNNNNGC 2 cut(s) 197, 329
NdeII GATC 2 cut(s) 54, 113
NlaIII CATG 1 cut(s) 89
PaeR7I CTCGAG 1 cut(s) 386
PagI TCATGA 1 cut(s) 85
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 2 cut(s) 281, 378
PpsI GAGTC 2 cut(s) 281, 378
PspFI CCCAGC 1 cut(s) 103
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 386
PsrI GAACNNNNNNTAC 2 cut(s) 81, 113
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 54, 113
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 282, 378
SetI ASST 8 cut(s) 31, 65, 85, 94, 109, 147, 234, 265
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 207
Sfr274I CTCGAG 1 cut(s) 386
SlaI CTCGAG 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 371, 386
SmoI CTYRAG 2 cut(s) 371, 386
Sse9I AATT 2 cut(s) 222, 301
SspMI CTAG 2 cut(s) 270, 329
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 2 cut(s) 85, 265
TaqI TCGA 4 cut(s) 57, 184, 359, 387
TasI AATT 2 cut(s) 222, 301
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 1 cut(s) 219
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 102
TspRI CASTG 1 cut(s) 219
Van91I CCANNNNNTGG 1 cut(s) 110
XhoI CTCGAG 1 cut(s) 386
XspI CTAG 2 cut(s) 270, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.