Rh4AG045500

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011610
Physical Location & Seq
Reverse (-)
9583341 .. 9584304
964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG045500.1

Sequence Viewer

Length: 564 bp
ATGTCCAAGATGGTAAATTTGTATCTCATGAGATTTCTTTCATCATGTTGGTGCAGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATGGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGTAGCAATGGAAGCAAGAGGTGACCTAGGTGATCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAACTATGTCGGCAGCTAAATAAGCCAGTCATTGTTGCTTCTCAACTACTCGAATATATGATCGAGTATCCTACACCCACTATAGCTGAAGTGGCTGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCAGAGGAGCTTTGTATTTCTGCTGCCAAGATGGAGGACGATGCACCAAGGCTGGTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

187

Amino Acids

20.89

Weight (kDa)

4.71

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 19 - 130 7.4e-38 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 476
AclWI GGATC 2 cut(s) 428, 487
AcsI RAATTY 1 cut(s) 16
AcuI CTGAAG 2 cut(s) 318, 336
AfiI CCNNNNNNNGG 1 cut(s) 476
AgsI TTSAA 1 cut(s) 212
AluBI AGCT 7 cut(s) 226, 296, 335, 365, 395, 473, 511
AluI AGCT 7 cut(s) 226, 296, 335, 365, 395, 473, 511
AlwI GGATC 2 cut(s) 428, 487
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 4 cut(s) 54, 223, 328, 524
ApoI RAATTY 1 cut(s) 16
AspA2I CCTAGG 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 184, 370
AsuHPI GGTGA 3 cut(s) 164, 173, 368
AvaII GGWCC 1 cut(s) 184
AvrII CCTAGG 1 cut(s) 157
BbvI GCAGC 4 cut(s) 66, 235, 340, 511
BccI CCATC 3 cut(s) 4, 196, 526
BceAI ACGGC 1 cut(s) 305
BciVI GTATCC 1 cut(s) 288
BclI TGATCA 1 cut(s) 163
BfaI CTAG 2 cut(s) 158, 562
BfmI CTRYAG 1 cut(s) 291
BfuI GTATCC 1 cut(s) 288
BisI GCNGC 4 cut(s) 55, 224, 329, 525
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 4 cut(s) 56, 225, 330, 526
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 184, 370
BmiI GGNNCC 1 cut(s) 186
BmsI GCATC 4 cut(s) 134, 328, 336, 532
BsaJI CCNNGG 2 cut(s) 157, 548
Bsc4I CCNNNNNNNGG 1 cut(s) 476
Bse1I ACTGG 2 cut(s) 236, 373
Bse3DI GCAATG 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 157, 548
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 1 cut(s) 415
BseNI ACTGG 2 cut(s) 236, 373
BseRI GAGGAG 1 cut(s) 521
BseXI GCAGC 4 cut(s) 66, 235, 340, 511
BseYI CCCAGC 2 cut(s) 195, 469
BsgI GTGCAG 1 cut(s) 73
BshFI GGCC 1 cut(s) 372
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 1 cut(s) 372
Bsp143I GATC 5 cut(s) 114, 163, 270, 420, 479
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 1 cut(s) 414
BspHI TCATGA 2 cut(s) 27, 451
BspLI GGNNCC 1 cut(s) 186
BspPI GGATC 2 cut(s) 428, 487
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 2 cut(s) 236, 373
BssECI CCNNGG 2 cut(s) 157, 548
BssMI GATC 5 cut(s) 114, 163, 270, 420, 479
BssT1I CCWWGG 2 cut(s) 157, 548
Bst4CI ACNGT 1 cut(s) 497
Bst6I CTCTTC 2 cut(s) 105, 435
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 401
BstEII GGTNACC 1 cut(s) 152
BstF5I GGATG 1 cut(s) 117
BstKTI GATC 5 cut(s) 117, 166, 273, 423, 482
BstMBI GATC 5 cut(s) 114, 163, 270, 420, 479
BstMWI GCNNNNNNNGC 3 cut(s) 143, 232, 302
BstPI GGTNACC 1 cut(s) 152
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 4 cut(s) 66, 235, 340, 511
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 1 cut(s) 372
BtsCI GGATG 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 333
CciI TCATGA 2 cut(s) 27, 451
Cfr13I GGNCC 2 cut(s) 184, 370
CviAII CATG 3 cut(s) 28, 45, 452
DdeI CTNAG 1 cut(s) 401
DpnI GATC 5 cut(s) 116, 165, 272, 422, 481
DpnII GATC 5 cut(s) 114, 163, 270, 420, 479
Eam1104I CTCTTC 2 cut(s) 105, 435
EarI CTCTTC 2 cut(s) 105, 435
Eco130I CCWWGG 2 cut(s) 157, 548
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 2 cut(s) 318, 336
Eco91I GGTNACC 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 184
EcoO65I GGTNACC 1 cut(s) 152
EcoT14I CCWWGG 2 cut(s) 157, 548
ErhI CCWWGG 2 cut(s) 157, 548
FaeI CATG 3 cut(s) 31, 48, 455
FaqI GGGAC 1 cut(s) 170
FatI CATG 3 cut(s) 27, 44, 451
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 4 cut(s) 55, 224, 329, 525
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 4 cut(s) 55, 224, 329, 525
FspBI CTAG 2 cut(s) 158, 562
GluI GCNGC 4 cut(s) 55, 224, 329, 525
GsaI CCCAGC 2 cut(s) 199, 473
HaeIII GGCC 1 cut(s) 372
Hin1II CATG 3 cut(s) 31, 48, 455
HinfI GANTC 2 cut(s) 92, 359
HphI GGTGA 3 cut(s) 164, 173, 368
Hpy188I TCNGA 7 cut(s) 130, 168, 316, 345, 404, 493, 505
Hpy188III TCNNGA 3 cut(s) 28, 424, 452
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4IV ACGT 1 cut(s) 448
HpyCH4V TGCA 3 cut(s) 54, 125, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 232, 302
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 448
Hsp92II CATG 3 cut(s) 31, 48, 455
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 5 cut(s) 114, 163, 270, 420, 479
LmnI GCTCC 1 cut(s) 508
LpnPI CCDG 8 cut(s) 167, 209, 249, 339, 386, 393, 483, 539
Lsp1109I GCAGC 4 cut(s) 66, 235, 340, 511
LweI GCATC 4 cut(s) 134, 328, 336, 532
MaeI CTAG 2 cut(s) 158, 562
MaeII ACGT 1 cut(s) 448
MaeIII GTNAC 2 cut(s) 152, 462
MalI GATC 5 cut(s) 116, 165, 272, 422, 481
MboI GATC 5 cut(s) 114, 163, 270, 420, 479
MboII GAAGA 2 cut(s) 122, 452
MluCI AATT 1 cut(s) 16
MlyI GAGTC 2 cut(s) 86, 368
MnlI CCTC 7 cut(s) 143, 318, 411, 420, 429, 499, 529
MslI CAYNNNNRTG 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 143, 232, 302
NdeII GATC 5 cut(s) 114, 163, 270, 420, 479
NlaIII CATG 3 cut(s) 31, 48, 455
NlaIV GGNNCC 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 152
PagI TCATGA 2 cut(s) 27, 451
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 4 cut(s) 56, 225, 330, 526
PleI GAGTC 2 cut(s) 86, 367
PpsI GAGTC 2 cut(s) 86, 367
PpuMI RGGWCCY 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 152
PspFI CCCAGC 2 cut(s) 195, 469
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 2 cut(s) 184, 370
PspPPI RGGWCCY 1 cut(s) 184
PsrI GAACNNNNNNTAC 2 cut(s) 447, 479
RseI CAYNNNNRTG 1 cut(s) 49
SatI GCNGC 4 cut(s) 55, 224, 329, 525
Sau3AI GATC 5 cut(s) 114, 163, 270, 420, 479
Sau96I GGNCC 2 cut(s) 184, 370
SchI GAGTC 2 cut(s) 86, 368
SfaNI GCATC 4 cut(s) 134, 328, 336, 532
SfcI CTRYAG 1 cut(s) 291
SinI GGWCC 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 49
Sse9I AATT 1 cut(s) 16
SspMI CTAG 2 cut(s) 158, 562
StyI CCWWGG 2 cut(s) 157, 548
TaaI ACNGT 1 cut(s) 497
TaiI ACGT 1 cut(s) 451
TaqI TCGA 3 cut(s) 261, 273, 423
TasI AATT 1 cut(s) 16
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 4 cut(s) 54, 223, 328, 524
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 30, 468
Van91I CCANNNNNTGG 1 cut(s) 476
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 1 cut(s) 16
XmaJI CCTAGG 1 cut(s) 157
XspI CTAG 2 cut(s) 158, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.