Rw7G041350

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011610
Physical Location & Seq
Reverse (-)
64453258 .. 64456958
3701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041350.1

Sequence Viewer

Length: 660 bp
ATGCACGATTTCCACTCAAAGGAAAATGGGGCTGAAGGTGTTGATTTCATTGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCACCTTAAAAGCTATATTGCAGCACGGTCTCGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACGATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATTGTTGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCTGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCTAGATAAGTCATTAGCTGTTCTCAGAAGTCCCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATTATATCAGAGGAGCTTTGTATTTCTGCTGCCAAGATGGGAAGAGCTATAATTCACACCTTGTGTACAATGGTGAAATCTTGTTTAGAGGATGAGAATCAGATGACCCAATTTTCGTCCTACATCACTTTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

219

Amino Acids

24.34

Weight (kDa)

5.62

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 12 - 148 1.9e-47 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 120
AclWI GGATC 1 cut(s) 491
AcuI CTGAAG 4 cut(s) 54, 96, 381, 399
AdeI CACNNNGTG 1 cut(s) 585
AfaI GTAC 1 cut(s) 589
AfiI CCNNNNNNNGG 2 cut(s) 19, 480
AgsI TTSAA 1 cut(s) 275
AluBI AGCT 8 cut(s) 99, 289, 359, 398, 428, 458, 538, 569
AluI AGCT 8 cut(s) 99, 289, 359, 398, 428, 458, 538, 569
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 1 cut(s) 120
AlwI GGATC 1 cut(s) 491
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 4 cut(s) 107, 286, 391, 551
ArsI GACNNNNNNTTYG 2 cut(s) 619, 651
AspA2I CCTAGG 1 cut(s) 220
AspS9I GGNCC 2 cut(s) 247, 433
AsuHPI GGTGA 4 cut(s) 80, 227, 431, 607
AvaII GGWCC 1 cut(s) 247
AvrII CCTAGG 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 4 cut(s) 119, 298, 403, 538
BccI CCATC 3 cut(s) 188, 259, 553
BceAI ACGGC 1 cut(s) 368
BciVI GTATCC 1 cut(s) 351
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 3 cut(s) 221, 354, 443
BfuI GTATCC 1 cut(s) 351
BisI GCNGC 4 cut(s) 108, 287, 392, 552
BlnI CCTAGG 1 cut(s) 220
BlsI GCNGC 4 cut(s) 109, 288, 393, 553
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 247, 433
BmiI GGNNCC 1 cut(s) 249
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 3 cut(s) 197, 391, 399
BmuI ACTGGG 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 618
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 480
Bse1I ACTGG 3 cut(s) 299, 436, 474
Bse3DI GCAATG 2 cut(s) 48, 207
Bse8I GATNNNNATC 1 cut(s) 618
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 2 cut(s) 180, 619
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 2 cut(s) 19, 480
BseMI GCAATG 2 cut(s) 48, 207
BseMII CTCAG 2 cut(s) 242, 478
BseNI ACTGG 3 cut(s) 299, 436, 474
BseRI GAGGAG 1 cut(s) 548
BseXI GCAGC 4 cut(s) 119, 298, 403, 538
BseYI CCCAGC 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 229
BslFI GGGAC 2 cut(s) 233, 456
BslI CCNNNNNNNGG 2 cut(s) 19, 480
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 233, 456
BsnI GGCC 1 cut(s) 435
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 229
Bsp1407I TGTACA 1 cut(s) 587
Bsp143I GATC 4 cut(s) 81, 177, 333, 483
Bsp68I TCGCGA 1 cut(s) 120
BspANI GGCC 1 cut(s) 435
BspCNI CTCAG 2 cut(s) 241, 477
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 1 cut(s) 491
BspQI GCTCTTC 1 cut(s) 559
BspTNI GGTCTC 1 cut(s) 120
BsrDI GCAATG 2 cut(s) 48, 207
BsrGI TGTACA 1 cut(s) 587
BsrI ACTGG 3 cut(s) 299, 436, 474
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 4 cut(s) 81, 177, 333, 483
BssT1I CCWWGG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 114
Bst6I CTCTTC 3 cut(s) 168, 498, 559
BstAUI TGTACA 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 2 cut(s) 228, 464
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 2 cut(s) 180, 619
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 4 cut(s) 84, 180, 336, 486
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 4 cut(s) 81, 177, 333, 483
BstMWI GCNNNNNNNGC 3 cut(s) 206, 295, 365
BstPI GGTNACC 1 cut(s) 215
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 119, 298, 403, 538
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 1 cut(s) 435
BtsCI GGATG 2 cut(s) 180, 619
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 396
CciI TCATGA 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 247, 433
Csp6I GTAC 1 cut(s) 588
CviAII CATG 2 cut(s) 515, 657
CviQI GTAC 1 cut(s) 588
DdeI CTNAG 2 cut(s) 228, 464
DpnI GATC 4 cut(s) 83, 179, 335, 485
DpnII GATC 4 cut(s) 81, 177, 333, 483
DraIII CACNNNGTG 1 cut(s) 585
Eam1104I CTCTTC 3 cut(s) 168, 498, 559
EarI CTCTTC 3 cut(s) 168, 498, 559
Eco130I CCWWGG 1 cut(s) 220
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 4 cut(s) 54, 96, 381, 399
Eco91I GGTNACC 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 2 cut(s) 518, 660
FaiI YATR 8 cut(s) 102, 280, 332, 431, 516, 527, 572, 658
FaqI GGGAC 2 cut(s) 233, 456
FatI CATG 2 cut(s) 514, 656
FbaI TGATCA 1 cut(s) 81
Fnu4HI GCNGC 4 cut(s) 108, 287, 392, 552
FokI GGATG 2 cut(s) 167, 626
Fsp4HI GCNGC 4 cut(s) 108, 287, 392, 552
FspBI CTAG 3 cut(s) 221, 354, 443
GluI GCNGC 4 cut(s) 108, 287, 392, 552
GsaI CCCAGC 1 cut(s) 262
HaeIII GGCC 1 cut(s) 435
Hin1II CATG 2 cut(s) 518, 660
HinfI GANTC 4 cut(s) 155, 326, 422, 619
HphI GGTGA 4 cut(s) 80, 227, 431, 607
Hpy166II GTNNAC 1 cut(s) 588
Hpy188I TCNGA 7 cut(s) 193, 231, 379, 408, 467, 532, 624
Hpy188III TCNNGA 3 cut(s) 119, 487, 515
Hpy8I GTNNAC 1 cut(s) 588
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 3 cut(s) 4, 107, 188
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 295, 365
HpyF3I CTNAG 2 cut(s) 228, 464
HpySE526I ACGT 1 cut(s) 511
Hsp92II CATG 2 cut(s) 518, 660
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 4 cut(s) 81, 177, 333, 483
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 2 cut(s) 197, 535
LpnPI CCDG 6 cut(s) 230, 272, 312, 402, 449, 487
Lsp1109I GCAGC 4 cut(s) 119, 298, 403, 538
LweI GCATC 3 cut(s) 197, 391, 399
MaeI CTAG 3 cut(s) 221, 354, 443
MaeII ACGT 1 cut(s) 511
MaeIII GTNAC 1 cut(s) 215
MalI GATC 4 cut(s) 83, 179, 335, 485
MboI GATC 4 cut(s) 81, 177, 333, 483
MboII GAAGA 3 cut(s) 185, 515, 576
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 2 cut(s) 573, 632
MlyI GAGTC 2 cut(s) 149, 431
MnlI CCTC 7 cut(s) 206, 381, 474, 483, 492, 526, 604
MseI TTAA 1 cut(s) 93
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 206, 295, 365
NdeII GATC 4 cut(s) 81, 177, 333, 483
NlaIII CATG 2 cut(s) 518, 660
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 215
NruI TCGCGA 1 cut(s) 120
PagI TCATGA 1 cut(s) 514
PciSI GCTCTTC 1 cut(s) 559
PfeI GAWTC 2 cut(s) 326, 619
PkrI GCNGC 4 cut(s) 109, 288, 393, 553
PleI GAGTC 2 cut(s) 149, 430
PpsI GAGTC 2 cut(s) 149, 430
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 247, 433
PspPPI RGGWCCY 1 cut(s) 247
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 1 cut(s) 589
RsaNI GTAC 1 cut(s) 588
SapI GCTCTTC 1 cut(s) 559
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 4 cut(s) 108, 287, 392, 552
Sau3AI GATC 4 cut(s) 81, 177, 333, 483
Sau96I GGNCC 2 cut(s) 247, 433
SchI GAGTC 2 cut(s) 149, 431
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 3 cut(s) 197, 391, 399
SinI GGWCC 1 cut(s) 247
Sse9I AATT 2 cut(s) 573, 632
SspMI CTAG 3 cut(s) 221, 354, 443
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 114
TaiI ACGT 1 cut(s) 514
TaqI TCGA 3 cut(s) 324, 336, 486
TasI AATT 2 cut(s) 573, 632
TatI WGTACW 1 cut(s) 587
TfiI GAWTC 2 cut(s) 326, 619
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 4 cut(s) 107, 286, 391, 551
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 37, 531
VpaK11BI GGWCC 1 cut(s) 247
XmaJI CCTAGG 1 cut(s) 220
XspI CTAG 3 cut(s) 221, 354, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.