Rh5AG178300

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011610
Physical Location & Seq
Forward (+)
20059830 .. 20091868
32039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG178300.1

Sequence Viewer

Length: 759 bp
ATGCACGATTTCCACTCAAAGGAAAATGGGGCTGAAGGTGTTGATTTCATTGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCACCTTAAAAGCTATATTGCAGCACGGTCTCGCGATATTTCTGTTGTGGCAAAGATAGGGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATTGTTGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCTGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAAATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACATCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGTACAGGAACTACAGAAAATAAAGGAAGAGTTGTTGGAGTTGAAAAGGAAGAGACCACTAGATCCCCTAGCTGAGGAACTTGACGAACAAAGGTTAGGATTAATGACTACTTTCATTCTTGCATTTCCTGCTTCACTATTGCTTTTGTTGATTGTTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

252

Amino Acids

27.87

Weight (kDa)

5.38

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 12 - 151 5.3e-49 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 539
AccII CGCG 1 cut(s) 120
AclWI GGATC 3 cut(s) 491, 550, 653
AcuI CTGAAG 4 cut(s) 54, 96, 381, 399
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 19, 539, 670
AgsI TTSAA 2 cut(s) 275, 640
AluBI AGCT 9 cut(s) 99, 289, 359, 398, 428, 458, 536, 574, 668
AluI AGCT 9 cut(s) 99, 289, 359, 398, 428, 458, 536, 574, 668
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 2 cut(s) 120, 643
AlwI GGATC 3 cut(s) 491, 550, 653
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 4 cut(s) 107, 286, 391, 587
AseI ATTAAT 1 cut(s) 698
AspA2I CCTAGG 1 cut(s) 220
AspS9I GGNCC 2 cut(s) 247, 433
AsuHPI GGTGA 3 cut(s) 80, 227, 431
AvaII GGWCC 1 cut(s) 247
AvrII CCTAGG 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 229
BbvCI CCTCAGC 1 cut(s) 669
BbvI GCAGC 4 cut(s) 119, 298, 403, 574
BccI CCATC 3 cut(s) 188, 259, 589
BceAI ACGGC 1 cut(s) 368
BciVI GTATCC 1 cut(s) 351
BclI TGATCA 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 120, 643
BfaI CTAG 4 cut(s) 221, 354, 656, 665
BfmI CTRYAG 1 cut(s) 608
BfuI GTATCC 1 cut(s) 351
BisI GCNGC 4 cut(s) 108, 287, 392, 588
BlnI CCTAGG 1 cut(s) 220
BlsI GCNGC 4 cut(s) 109, 288, 393, 589
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 247, 433
BmiI GGNNCC 1 cut(s) 249
BmsI GCATC 3 cut(s) 197, 391, 399
Bpu10I CCTNAGC 1 cut(s) 669
BsaI GGTCTC 2 cut(s) 120, 643
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 539, 670
Bse1I ACTGG 2 cut(s) 299, 436
Bse3DI GCAATG 2 cut(s) 48, 207
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 3 cut(s) 19, 539, 670
BseMI GCAATG 2 cut(s) 48, 207
BseMII CTCAG 3 cut(s) 242, 478, 660
BseNI ACTGG 2 cut(s) 299, 436
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 4 cut(s) 119, 298, 403, 574
BseYI CCCAGC 2 cut(s) 258, 532
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 229
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 3 cut(s) 19, 539, 670
BsmAI GTCTC 2 cut(s) 120, 643
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 1 cut(s) 435
Bso31I GGTCTC 2 cut(s) 120, 643
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 6 cut(s) 81, 177, 333, 483, 542, 658
Bsp68I TCGCGA 1 cut(s) 120
BspANI GGCC 1 cut(s) 435
BspCNI CTCAG 3 cut(s) 241, 477, 661
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 3 cut(s) 491, 550, 653
BspTNI GGTCTC 2 cut(s) 120, 643
BsrDI GCAATG 2 cut(s) 48, 207
BsrI ACTGG 2 cut(s) 299, 436
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 6 cut(s) 81, 177, 333, 483, 542, 658
BssT1I CCWWGG 1 cut(s) 220
Bst4CI ACNGT 2 cut(s) 114, 560
Bst6I CTCTTC 4 cut(s) 168, 498, 618, 641
BstAPI GCANNNNNTGC 1 cut(s) 725
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 3 cut(s) 228, 464, 669
BstEII GGTNACC 1 cut(s) 215
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 1 cut(s) 180
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 6 cut(s) 84, 180, 336, 486, 545, 661
BstMAI GTCTC 2 cut(s) 120, 643
BstMBI GATC 6 cut(s) 81, 177, 333, 483, 542, 658
BstMWI GCNNNNNNNGC 4 cut(s) 206, 295, 365, 725
BstPI GGTNACC 1 cut(s) 215
BstSFI CTRYAG 1 cut(s) 608
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 119, 298, 403, 574
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 1 cut(s) 435
BtsCI GGATG 1 cut(s) 180
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 396
CciI TCATGA 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 247, 433
Csp6I GTAC 1 cut(s) 599
CviAII CATG 1 cut(s) 515
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 3 cut(s) 228, 464, 669
DpnI GATC 6 cut(s) 83, 179, 335, 485, 544, 660
DpnII GATC 6 cut(s) 81, 177, 333, 483, 542, 658
Eam1104I CTCTTC 4 cut(s) 168, 498, 618, 641
EarI CTCTTC 4 cut(s) 168, 498, 618, 641
Eco130I CCWWGG 1 cut(s) 220
Eco31I GGTCTC 2 cut(s) 120, 643
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 4 cut(s) 54, 96, 381, 399
Eco91I GGTNACC 1 cut(s) 215
EcoNI CCTNNNNNAGG 1 cut(s) 668
EcoO109I RGGNCCY 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 1 cut(s) 518
FaiI YATR 7 cut(s) 102, 280, 332, 431, 516, 524, 563
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 514
FbaI TGATCA 1 cut(s) 81
Fnu4HI GCNGC 4 cut(s) 108, 287, 392, 588
FokI GGATG 1 cut(s) 167
Fsp4HI GCNGC 4 cut(s) 108, 287, 392, 588
FspBI CTAG 4 cut(s) 221, 354, 656, 665
GluI GCNGC 4 cut(s) 108, 287, 392, 588
GsaI CCCAGC 2 cut(s) 262, 536
HaeIII GGCC 1 cut(s) 435
Hin1II CATG 1 cut(s) 518
HinfI GANTC 3 cut(s) 155, 326, 422
HphI GGTGA 3 cut(s) 80, 227, 431
Hpy188I TCNGA 7 cut(s) 193, 231, 379, 408, 467, 556, 568
Hpy188III TCNNGA 3 cut(s) 119, 487, 515
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 2 cut(s) 114, 560
HpyCH4V TGCA 4 cut(s) 4, 107, 188, 719
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 295, 365, 725
HpyF3I CTNAG 3 cut(s) 228, 464, 669
Hsp92II CATG 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 6 cut(s) 81, 177, 333, 483, 542, 658
LmnI GCTCC 2 cut(s) 197, 571
LpnPI CCDG 8 cut(s) 230, 272, 312, 402, 449, 546, 587, 738
Lsp1109I GCAGC 4 cut(s) 119, 298, 403, 574
LweI GCATC 3 cut(s) 197, 391, 399
MaeI CTAG 4 cut(s) 221, 354, 656, 665
MaeIII GTNAC 2 cut(s) 215, 525
MalI GATC 6 cut(s) 83, 179, 335, 485, 544, 660
MboI GATC 6 cut(s) 81, 177, 333, 483, 542, 658
MboII GAAGA 4 cut(s) 185, 515, 635, 658
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 229
MlyI GAGTC 2 cut(s) 149, 431
MmeI TCCRAC 1 cut(s) 612
MnlI CCTC 7 cut(s) 206, 381, 474, 483, 492, 562, 664
MseI TTAA 2 cut(s) 93, 698
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 4 cut(s) 206, 295, 365, 725
NdeII GATC 6 cut(s) 81, 177, 333, 483, 542, 658
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 215
NruI TCGCGA 1 cut(s) 120
PagI TCATGA 1 cut(s) 514
PfeI GAWTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 4 cut(s) 109, 288, 393, 589
PleI GAGTC 2 cut(s) 149, 430
PpsI GAGTC 2 cut(s) 149, 430
PpuMI RGGWCCY 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 698
Psp5II RGGWCCY 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 215
PspFI CCCAGC 2 cut(s) 258, 532
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 247, 433
PspPPI RGGWCCY 1 cut(s) 247
PsrI GAACNNNNNNTAC 2 cut(s) 510, 542
PsuI RGATCY 1 cut(s) 658
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
SaqAI TTAA 2 cut(s) 93, 698
SatI GCNGC 4 cut(s) 108, 287, 392, 588
Sau3AI GATC 6 cut(s) 81, 177, 333, 483, 542, 658
Sau96I GGNCC 2 cut(s) 247, 433
SchI GAGTC 2 cut(s) 149, 431
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 3 cut(s) 197, 391, 399
SfcI CTRYAG 1 cut(s) 608
SinI GGWCC 1 cut(s) 247
SspMI CTAG 4 cut(s) 221, 354, 656, 665
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 2 cut(s) 114, 560
TaqI TCGA 3 cut(s) 324, 336, 486
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 2 cut(s) 93, 698
Tru9I TTAA 2 cut(s) 93, 698
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 4 cut(s) 107, 286, 391, 587
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 3 cut(s) 37, 531, 700
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 247
VspI ATTAAT 1 cut(s) 698
XagI CCTNNNNNAGG 1 cut(s) 668
XmaJI CCTAGG 1 cut(s) 220
XspI CTAG 4 cut(s) 221, 354, 656, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.