Rh1AG185300

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0015761
Physical Location & Seq
Forward (+)
35303507 .. 35308470
4964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG185300.1

Sequence Viewer

Length: 324 bp
ATGGTTAAAAATAAAGGCTTCTTGTCGTCAGGTCCTTCGGAGATTCCTATTCAGCGTAACCAAATAAAGGAAATTATATATTCCTTACTTCCAGCTTGCAAAGAGCCTGATGTAGATTCTGGTATTCCCTTTAAAGCGGATGCAATTATTGCAAACCCCCCAGCATATGGGCATACTCATGTGGTGGAGGCACTGAAAATACCTCTTCATATATTTTTTACAATGCCATGGACGCAAGTTGAGGGGGAAACACGTGTCGGTGTGTTTGCTGCAAGATCAATAGAAGTCGGAGAGCCATTAACATATGATTATAGGTATCCATGA

Protein Analysis

107

Amino Acids

11.93

Weight (kDa)

5.86

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 5 - 78 7.6e-12 Glycosyltransferase family 28 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 167
AciI CCGC 1 cut(s) 137
AcvI CACGTG 1 cut(s) 254
AfiI CCNNNNNNNGG 2 cut(s) 67, 167
AflIII ACRYGT 2 cut(s) 251, 253
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 32
AvaII GGWCC 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 254
BbvI GCAGC 1 cut(s) 256
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 32
BmsI GCATC 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 227
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 167
BseDI CCNNGG 1 cut(s) 227
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 2 cut(s) 67, 167
BseXI GCAGC 1 cut(s) 256
BseYI CCCAGC 1 cut(s) 160
BslI CCNNNNNNNGG 2 cut(s) 67, 167
Bsp143I GATC 1 cut(s) 275
Bsp19I CCATGG 1 cut(s) 227
BspACI CCGC 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 1 cut(s) 275
BssT1I CCWWGG 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 210
BstAPI GCANNNNNTGC 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 97
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 1 cut(s) 278
BstMBI GATC 1 cut(s) 275
BstMWI GCNNNNNNNGC 2 cut(s) 149, 232
BstV1I GCAGC 1 cut(s) 256
BtgI CCRYGG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 191
Cac8I GCNNGC 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 32
CseI GACGC 1 cut(s) 241
CviAII CATG 3 cut(s) 179, 228, 321
CviJI RGCY 4 cut(s) 18, 95, 106, 295
CviKI_1 RGCY 4 cut(s) 18, 95, 106, 295
DpnI GATC 1 cut(s) 277
DpnII GATC 1 cut(s) 275
DraI TTTAAA 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 32
Eco72I CACGTG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 3 cut(s) 182, 231, 324
FatI CATG 3 cut(s) 178, 227, 320
FauNDI CATATG 2 cut(s) 166, 304
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 270
GsaI CCCAGC 1 cut(s) 164
HgaI GACGC 1 cut(s) 241
Hin1II CATG 3 cut(s) 182, 231, 324
HinfI GANTC 2 cut(s) 43, 116
Hpy188I TCNGA 2 cut(s) 40, 290
HpyAV CCTTC 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 4 cut(s) 99, 143, 152, 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 232
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 3 cut(s) 182, 231, 324
Kzo9I GATC 1 cut(s) 275
LpnPI CCDG 5 cut(s) 15, 105, 105, 120, 174
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 130
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 1 cut(s) 56
MalI GATC 1 cut(s) 277
MboI GATC 1 cut(s) 275
MboII GAAGA 1 cut(s) 197
MluCI AATT 2 cut(s) 72, 144
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 3 cut(s) 181, 213, 235
MseI TTAA 3 cut(s) 6, 132, 299
MslI CAYNNNNRTG 1 cut(s) 177
MwoI GCNNNNNNNGC 2 cut(s) 149, 232
NcoI CCATGG 1 cut(s) 227
NdeI CATATG 2 cut(s) 166, 304
NdeII GATC 1 cut(s) 275
NlaIII CATG 3 cut(s) 182, 231, 324
PfeI GAWTC 2 cut(s) 43, 116
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 1 cut(s) 271
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
Ppu21I YACGTR 1 cut(s) 254
PpuMI RGGWCCY 1 cut(s) 32
Psp5II RGGWCCY 1 cut(s) 32
PspCI CACGTG 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 32
PspPPI RGGWCCY 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 3 cut(s) 6, 132, 299
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 1 cut(s) 275
Sau96I GGNCC 1 cut(s) 32
SetI ASST 5 cut(s) 34, 97, 205, 256, 317
SfaNI GCATC 1 cut(s) 130
SinI GGWCC 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 2 cut(s) 72, 144
SsiI CCGC 1 cut(s) 137
StyI CCWWGG 1 cut(s) 227
TaiI ACGT 1 cut(s) 256
TasI AATT 2 cut(s) 72, 144
TfiI GAWTC 2 cut(s) 43, 116
Tru1I TTAA 3 cut(s) 6, 132, 299
Tru9I TTAA 3 cut(s) 6, 132, 299
TscAI CASTG 1 cut(s) 198
TseI GCWGC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 197
TspRI CASTG 1 cut(s) 198
Van91I CCANNNNNTGG 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.