Rh2AG237400

Haloacid dehalogenase-like hydrolase domain-containing protein At4g39970

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007382
Physical Location & Seq
Forward (+)
25673825 .. 25676752
2928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG237400.1

Sequence Viewer

Length: 708 bp
ATGGCGTTCACCATGGCTCTCTCTTCCCCCTCCTCCTCTTTTCTACTCTCCCACACCACCCGCCTTCACTTCCGAAGCCCCAGAAACCTCTCCACCCCCACACGACGACCTCTTTCACTCTCAGTCTCAGCTTCTTCCTCTGCCTCTCTCCAAGCCTTGATTTTCGACTGCGACGGCGTCATCCTCGAGTCGGAGCACTTGCACCGCCAAGCTTACAACGACGCCTTCGCTCACTTCAACGTCCGCTGCTCCTCTCAGACCCTCAATTGGGACCCCGACTTCTACGACCAGCTCCAGAACCAAATCGGCGGCGGCAAGCCCAAAATGCGATGGTACTTTAAGGAGAACGGGTGGCCGTCGTCCACCATTTTCGAGAAGCCTCCAGAAGACGATGATGGCCGAGCCAAGATCATCGACACTCTTCAGGATTGGAAAACTGAGAGGTACAAAGAAATTATCAAGTCCGGATCTGTGGAACCAAGACCAGGAGTTTTGAGATTAATGGATGAGGCCAAGGCCGCTGGGAAAAAGCTCGCTGTTTGCTCTGCAGCTACTAAGAGTTCAGTTATATTATGTCTTGAAAGCCTTATAGGAAATGAGCGGTTTCAAGGTCTTGATTGCTTTCTTGCTGGATCACGTCAATTACGCATGATTGATATTCCAGCTCCATTATATTATAATTTGCTGCATTCTTGCCTAGAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

235

Amino Acids

26.35

Weight (kDa)

7.64

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 51 - 202 8.8e-11 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AccBSI CCGCTC 1 cut(s) 601
AccIII TCCGGA 1 cut(s) 464
AciI CCGC 7 cut(s) 61, 205, 244, 309, 312, 519, 601
AclWI GGATC 2 cut(s) 475, 640
AcoI YGGCCR 2 cut(s) 353, 397
AcuI CTGAAG 1 cut(s) 407
AcyI GRCGYC 2 cut(s) 177, 222
AfaI GTAC 2 cut(s) 335, 446
AfiI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
AgsI TTSAA 3 cut(s) 238, 581, 608
AjiI CACGTC 1 cut(s) 638
AjnI CCWGG 1 cut(s) 484
AluBI AGCT 6 cut(s) 131, 212, 292, 532, 551, 665
AluI AGCT 6 cut(s) 131, 212, 292, 532, 551, 665
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 2 cut(s) 475, 640
Ama87I CYCGRG 1 cut(s) 185
Aor13HI TCCGGA 1 cut(s) 464
AoxI GGCC 4 cut(s) 353, 397, 510, 516
ApeKI GCWGC 3 cut(s) 246, 548, 685
AseI ATTAAT 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 233, 560, 672
BccI CCATC 2 cut(s) 324, 389
BceAI ACGGC 2 cut(s) 190, 340
BciT130I CCWGG 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 130
BfaI CTAG 1 cut(s) 698
BfmI CTRYAG 1 cut(s) 546
BisI GCNGC 6 cut(s) 247, 310, 313, 519, 549, 686
BlsI GCNGC 6 cut(s) 248, 311, 314, 520, 550, 687
Bme1390I CCNGG 1 cut(s) 486
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 638
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 3 cut(s) 272, 273, 477
BmrFI CCNGG 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 2 cut(s) 278, 366
BsaHI GRCGYC 2 cut(s) 177, 222
BsaJI CCNNGG 2 cut(s) 12, 513
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
BseAI TCCGGA 1 cut(s) 464
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 12, 513
BseGI GGATG 2 cut(s) 180, 511
BseLI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
BseMII CTCAG 4 cut(s) 135, 141, 269, 429
BseRI GAGGAG 3 cut(s) 22, 25, 241
BseXI GCAGC 3 cut(s) 233, 560, 672
BseYI CCCAGC 1 cut(s) 521
BshFI GGCC 4 cut(s) 355, 399, 512, 518
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 465
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 284
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 4 cut(s) 355, 399, 512, 518
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 464
Bsp143I GATC 3 cut(s) 408, 467, 632
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 7 cut(s) 61, 205, 244, 309, 312, 519, 601
BspANI GGCC 4 cut(s) 355, 399, 512, 518
BspCNI CTCAG 4 cut(s) 134, 140, 268, 430
BspEI TCCGGA 1 cut(s) 464
BspLI GGNNCC 3 cut(s) 272, 273, 477
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 2 cut(s) 475, 640
BsrBI CCGCTC 1 cut(s) 601
BssECI CCNNGG 2 cut(s) 12, 513
BssMI GATC 3 cut(s) 408, 467, 632
BssNI GRCGYC 2 cut(s) 177, 222
BssT1I CCWWGG 2 cut(s) 12, 513
Bst2UI CCWGG 1 cut(s) 486
Bst6I CTCTTC 2 cut(s) 28, 426
BstACI GRCGYC 2 cut(s) 177, 222
BstC8I GCNNGC 2 cut(s) 317, 534
BstDEI CTNAG 5 cut(s) 121, 127, 255, 438, 555
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 180, 511
BstKTI GATC 3 cut(s) 411, 470, 635
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 3 cut(s) 408, 467, 632
BstMWI GCNNNNNNNGC 2 cut(s) 325, 518
BstNI CCWGG 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 546
BstV1I GCAGC 3 cut(s) 233, 560, 672
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 4 cut(s) 355, 399, 512, 518
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 343
BtrI CACGTC 1 cut(s) 638
BtsCI GGATG 2 cut(s) 180, 511
Cac8I GCNNGC 2 cut(s) 317, 534
Cfr13I GGNCC 1 cut(s) 271
CseI GACGC 2 cut(s) 166, 230
Csp6I GTAC 2 cut(s) 334, 445
CviAII CATG 2 cut(s) 13, 649
CviQI GTAC 2 cut(s) 334, 445
DdeI CTNAG 5 cut(s) 121, 127, 255, 438, 555
DpnI GATC 3 cut(s) 410, 469, 634
DpnII GATC 3 cut(s) 408, 467, 632
EaeI YGGCCR 2 cut(s) 353, 397
Eam1104I CTCTTC 2 cut(s) 28, 426
EarI CTCTTC 2 cut(s) 28, 426
Eco130I CCWWGG 2 cut(s) 12, 513
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 2 cut(s) 12, 513
ErhI CCWWGG 2 cut(s) 12, 513
FaeI CATG 2 cut(s) 16, 652
FaiI YATR 7 cut(s) 14, 569, 574, 590, 650, 673, 678
FaqI GGGAC 1 cut(s) 284
FatI CATG 2 cut(s) 12, 648
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 6 cut(s) 247, 310, 313, 519, 549, 686
FokI GGATG 2 cut(s) 167, 518
Fsp4HI GCNGC 6 cut(s) 247, 310, 313, 519, 549, 686
FspBI CTAG 1 cut(s) 698
GluI GCNGC 6 cut(s) 247, 310, 313, 519, 549, 686
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 2 cut(s) 278, 366
HaeIII GGCC 4 cut(s) 355, 399, 512, 518
HapII CCGG 1 cut(s) 465
HgaI GACGC 2 cut(s) 166, 230
Hin1I GRCGYC 2 cut(s) 177, 222
Hin1II CATG 2 cut(s) 16, 652
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 465
Hpy166II GTNNAC 2 cut(s) 9, 363
Hpy188I TCNGA 3 cut(s) 74, 193, 258
Hpy188III TCNNGA 7 cut(s) 295, 373, 383, 425, 465, 578, 614
Hpy8I GTNNAC 2 cut(s) 9, 363
Hpy99I CGWCG 4 cut(s) 108, 176, 224, 361
HpyAV CCTTC 2 cut(s) 74, 235
HpyCH4IV ACGT 2 cut(s) 240, 637
HpyCH4V TGCA 3 cut(s) 202, 548, 688
HpyF10VI GCNNNNNNNGC 2 cut(s) 325, 518
HpyF3I CTNAG 5 cut(s) 121, 127, 255, 438, 555
HpySE526I ACGT 2 cut(s) 240, 637
Hsp92I GRCGYC 2 cut(s) 177, 222
Hsp92II CATG 2 cut(s) 16, 652
KflI GGGWCCC 1 cut(s) 271
Kpn2I TCCGGA 1 cut(s) 464
Kzo9I GATC 3 cut(s) 408, 467, 632
LmnI GCTCC 4 cut(s) 193, 254, 297, 670
Lsp1109I GCAGC 3 cut(s) 233, 560, 672
MaeI CTAG 1 cut(s) 698
MaeII ACGT 2 cut(s) 240, 637
MalI GATC 3 cut(s) 410, 469, 634
MbiI CCGCTC 1 cut(s) 601
MboI GATC 3 cut(s) 408, 467, 632
MboII GAAGA 4 cut(s) 15, 126, 398, 413
MfeI CAATTG 1 cut(s) 265
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 4 cut(s) 265, 453, 641, 679
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 171
MroI TCCGGA 1 cut(s) 464
MseI TTAA 2 cut(s) 339, 500
MspA1I CMGCKG 2 cut(s) 246, 521
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 1 cut(s) 486
MunI CAATTG 1 cut(s) 265
Mva1269I GAATGC 1 cut(s) 688
MvaI CCWGG 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 325, 518
NcoI CCATGG 1 cut(s) 12
NdeII GATC 3 cut(s) 408, 467, 632
NlaIII CATG 2 cut(s) 16, 652
NlaIV GGNNCC 3 cut(s) 272, 273, 477
NmeAIII GCCGAG 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 185
PctI GAATGC 1 cut(s) 688
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 6 cut(s) 248, 311, 314, 520, 550, 687
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 271
PshBI ATTAAT 1 cut(s) 500
PsiI TTATAA 1 cut(s) 678
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 484
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 3 cut(s) 272, 273, 477
PspPI GGNCC 1 cut(s) 271
PspPPI RGGWCCY 1 cut(s) 271
PspXI VCTCGAGB 1 cut(s) 185
PstI CTGCAG 1 cut(s) 550
PsuI RGATCY 1 cut(s) 467
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 2 cut(s) 335, 446
RsaNI GTAC 2 cut(s) 334, 445
SaqAI TTAA 2 cut(s) 339, 500
SatI GCNGC 6 cut(s) 247, 310, 313, 519, 549, 686
Sau3AI GATC 3 cut(s) 408, 467, 632
Sau96I GGNCC 1 cut(s) 271
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 1 cut(s) 198
SfcI CTRYAG 1 cut(s) 546
Sfr274I CTCGAG 1 cut(s) 185
SinI GGWCC 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 4 cut(s) 265, 453, 641, 679
SsiI CCGC 7 cut(s) 61, 205, 244, 309, 312, 519, 601
SspMI CTAG 1 cut(s) 698
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 2 cut(s) 12, 513
TaiI ACGT 2 cut(s) 243, 640
TaqI TCGA 4 cut(s) 165, 186, 372, 414
TasI AATT 4 cut(s) 265, 453, 641, 679
TauI GCSGC 3 cut(s) 312, 315, 521
Tru1I TTAA 2 cut(s) 339, 500
Tru9I TTAA 2 cut(s) 339, 500
TseI GCWGC 3 cut(s) 246, 548, 685
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 271
VspI ATTAAT 1 cut(s) 500
XhoI CTCGAG 1 cut(s) 185
XspI CTAG 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.