Rh2BG250100

Haloacid dehalogenase-like hydrolase domain-containing protein At4g39970

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007382
Physical Location & Seq
Forward (+)
25636513 .. 25639182
2670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG250100.1

Sequence Viewer

Length: 678 bp
ATGGCGTTCACCATGGCTCTCTCTTCCCCCTCCTCCTCTTTTCTACTCTCCCACACCACCCGCCTTCACTTCCGAAGCCCCAGAAACCTCTCCACCCCCACACGACGACCTCTTTCACTCTCAGTCTCAGCTTCTTCCTCTGCTGCTCTCCAAGCCTTGATTTTCGACTGCGACGGCGTCATCCTCGAGTCGGAGCACTTGCACCGCCAAGCTTACAACGACGCCTTCGCTCACTTCAACGTCCGCTGCTCCTCTCAGACCCTCAATTGGGACCCCGACTTCTACGACCAGCTCCAGAACCAAATCGGCGGCGGCAAGCCCAAAATGCGATGGTACTTCAAGGAGAACGGGTGGCCGTCGTCCACCATTTTCGAGAAGCCTCCAGAAGACGATGATGGCCGAGCCAAGATCATCGACACTCTTCAGGATTGGAAAACTGAGAGGTACAAAGAAATTATCAAGTCCGGATCTGTGGAACCAAGACCAGGAGTTTTGAGATTAATGGACGAGGCCAAGGCCGCTGCAGCAACAAAAGCTGGGATGTCATGTGTGATTAGCTATACATCTTCTACAGCTGACCAGGATTTTAAAGATGCAATAGCAATATATCCTGACTTGAGTAACATAAGACTGAAAGACTTGGAATTGTTACTTGAAAATGCTGTCATTGCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

225

Amino Acids

25.17

Weight (kDa)

5.96

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 51 - 180 7.8e-07 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 464
AciI CCGC 6 cut(s) 61, 205, 244, 309, 312, 519
AclWI GGATC 1 cut(s) 475
AcoI YGGCCR 2 cut(s) 353, 397
AcuI CTGAAG 1 cut(s) 407
AcyI GRCGYC 2 cut(s) 177, 222
AfaI GTAC 2 cut(s) 335, 446
AfiI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
AgsI TTSAA 3 cut(s) 238, 340, 656
AjnI CCWGG 2 cut(s) 484, 579
AluBI AGCT 6 cut(s) 131, 212, 292, 536, 558, 575
AluI AGCT 6 cut(s) 131, 212, 292, 536, 558, 575
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 1 cut(s) 475
Ama87I CYCGRG 1 cut(s) 185
Aor13HI TCCGGA 1 cut(s) 464
AoxI GGCC 4 cut(s) 353, 397, 510, 516
ApeKI GCWGC 4 cut(s) 143, 246, 521, 524
AseI ATTAAT 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 4 cut(s) 130, 233, 508, 536
BccI CCATC 2 cut(s) 324, 389
BceAI ACGGC 2 cut(s) 190, 340
BciT130I CCWGG 2 cut(s) 486, 581
BcoDI GTCTC 1 cut(s) 130
BfmI CTRYAG 2 cut(s) 522, 570
BisI GCNGC 7 cut(s) 144, 247, 310, 313, 519, 522, 525
BlsI GCNGC 7 cut(s) 145, 248, 311, 314, 520, 523, 526
Bme1390I CCNGG 2 cut(s) 486, 581
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 3 cut(s) 272, 273, 477
BmrFI CCNGG 2 cut(s) 486, 581
BmsI GCATC 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 2 cut(s) 278, 366
BpuEI CTTGAG 1 cut(s) 637
BsaHI GRCGYC 2 cut(s) 177, 222
BsaJI CCNNGG 2 cut(s) 12, 513
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
Bse3DI GCAATG 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 464
BseBI CCWGG 2 cut(s) 486, 581
BseDI CCNNGG 2 cut(s) 12, 513
BseGI GGATG 2 cut(s) 180, 546
BseLI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 4 cut(s) 135, 141, 269, 429
BseRI GAGGAG 3 cut(s) 22, 25, 241
BseXI GCAGC 4 cut(s) 130, 233, 508, 536
BseYI CCCAGC 1 cut(s) 536
BshFI GGCC 4 cut(s) 355, 399, 512, 518
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 465
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 4 cut(s) 355, 399, 512, 518
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 464
Bsp143I GATC 2 cut(s) 408, 467
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 6 cut(s) 61, 205, 244, 309, 312, 519
BspANI GGCC 4 cut(s) 355, 399, 512, 518
BspCNI CTCAG 4 cut(s) 134, 140, 268, 430
BspEI TCCGGA 1 cut(s) 464
BspLI GGNNCC 3 cut(s) 272, 273, 477
BspMAI CTGCAG 1 cut(s) 526
BspPI GGATC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 666
BssECI CCNNGG 2 cut(s) 12, 513
BssMI GATC 2 cut(s) 408, 467
BssNI GRCGYC 2 cut(s) 177, 222
BssT1I CCWWGG 2 cut(s) 12, 513
Bst2UI CCWGG 2 cut(s) 486, 581
Bst6I CTCTTC 2 cut(s) 28, 426
BstACI GRCGYC 2 cut(s) 177, 222
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 4 cut(s) 121, 127, 255, 438
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 180, 546
BstKTI GATC 2 cut(s) 411, 470
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 2 cut(s) 408, 467
BstMWI GCNNNNNNNGC 6 cut(s) 152, 325, 518, 524, 533, 668
BstNI CCWGG 2 cut(s) 486, 581
BstSCI CCNGG 2 cut(s) 484, 579
BstSFI CTRYAG 2 cut(s) 522, 570
BstV1I GCAGC 4 cut(s) 130, 233, 508, 536
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 4 cut(s) 355, 399, 512, 518
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 343
BtsCI GGATG 2 cut(s) 180, 546
Cac8I GCNNGC 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 271
CseI GACGC 2 cut(s) 166, 230
Csp6I GTAC 2 cut(s) 334, 445
CviAII CATG 2 cut(s) 13, 546
CviQI GTAC 2 cut(s) 334, 445
DdeI CTNAG 4 cut(s) 121, 127, 255, 438
DpnI GATC 2 cut(s) 410, 469
DpnII GATC 2 cut(s) 408, 467
DraI TTTAAA 1 cut(s) 589
EaeI YGGCCR 2 cut(s) 353, 397
Eam1104I CTCTTC 2 cut(s) 28, 426
EarI CTCTTC 2 cut(s) 28, 426
Eco130I CCWWGG 2 cut(s) 12, 513
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 484, 579
EcoT14I CCWWGG 2 cut(s) 12, 513
ErhI CCWWGG 2 cut(s) 12, 513
FaeI CATG 2 cut(s) 16, 549
FaiI YATR 5 cut(s) 14, 547, 561, 607, 626
FaqI GGGAC 1 cut(s) 284
FatI CATG 2 cut(s) 12, 545
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 7 cut(s) 144, 247, 310, 313, 519, 522, 525
FokI GGATG 2 cut(s) 167, 553
Fsp4HI GCNGC 7 cut(s) 144, 247, 310, 313, 519, 522, 525
GluI GCNGC 7 cut(s) 144, 247, 310, 313, 519, 522, 525
GsaI CCCAGC 1 cut(s) 540
GsuI CTGGAG 2 cut(s) 278, 366
HaeIII GGCC 4 cut(s) 355, 399, 512, 518
HapII CCGG 1 cut(s) 465
HgaI GACGC 2 cut(s) 166, 230
Hin1I GRCGYC 2 cut(s) 177, 222
Hin1II CATG 2 cut(s) 16, 549
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 465
Hpy166II GTNNAC 2 cut(s) 9, 363
Hpy188I TCNGA 3 cut(s) 74, 193, 258
Hpy188III TCNNGA 6 cut(s) 295, 373, 383, 425, 465, 611
Hpy8I GTNNAC 2 cut(s) 9, 363
Hpy99I CGWCG 4 cut(s) 108, 176, 224, 361
HpyAV CCTTC 2 cut(s) 74, 235
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 202, 524, 596
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 325, 518, 524, 533, 668
HpyF3I CTNAG 4 cut(s) 121, 127, 255, 438
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 2 cut(s) 177, 222
Hsp92II CATG 2 cut(s) 16, 549
KflI GGGWCCC 1 cut(s) 271
Kpn2I TCCGGA 1 cut(s) 464
Kzo9I GATC 2 cut(s) 408, 467
LmnI GCTCC 3 cut(s) 193, 254, 297
Lsp1109I GCAGC 4 cut(s) 130, 233, 508, 536
LweI GCATC 1 cut(s) 583
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 2 cut(s) 620, 648
MalI GATC 2 cut(s) 410, 469
MboI GATC 2 cut(s) 408, 467
MboII GAAGA 5 cut(s) 15, 126, 398, 413, 558
MfeI CAATTG 1 cut(s) 265
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 4 cut(s) 265, 453, 644, 673
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 171
MroI TCCGGA 1 cut(s) 464
MseI TTAA 2 cut(s) 500, 588
MspA1I CMGCKG 3 cut(s) 246, 521, 575
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 2 cut(s) 486, 581
MunI CAATTG 1 cut(s) 265
MvaI CCWGG 2 cut(s) 486, 581
MwoI GCNNNNNNNGC 6 cut(s) 152, 325, 518, 524, 533, 668
NcoI CCATGG 1 cut(s) 12
NdeII GATC 2 cut(s) 408, 467
NlaIII CATG 2 cut(s) 16, 549
NlaIV GGNNCC 3 cut(s) 272, 273, 477
NmeAIII GCCGAG 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 185
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 7 cut(s) 145, 248, 311, 314, 520, 523, 526
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 271
PshBI ATTAAT 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 2 cut(s) 484, 579
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 2 cut(s) 484, 579
PspN4I GGNNCC 3 cut(s) 272, 273, 477
PspPI GGNCC 1 cut(s) 271
PspPPI RGGWCCY 1 cut(s) 271
PspXI VCTCGAGB 1 cut(s) 185
PstI CTGCAG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 467
PsyI GACNNNGTC 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 335, 446
RsaNI GTAC 2 cut(s) 334, 445
SaqAI TTAA 2 cut(s) 500, 588
SatI GCNGC 7 cut(s) 144, 247, 310, 313, 519, 522, 525
Sau3AI GATC 2 cut(s) 408, 467
Sau96I GGNCC 1 cut(s) 271
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 486, 581
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 2 cut(s) 522, 570
Sfr274I CTCGAG 1 cut(s) 185
SinI GGWCC 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 185, 616
SmoI CTYRAG 2 cut(s) 185, 616
Sse9I AATT 4 cut(s) 265, 453, 644, 673
SsiI CCGC 6 cut(s) 61, 205, 244, 309, 312, 519
StyD4I CCNGG 2 cut(s) 484, 579
StyI CCWWGG 2 cut(s) 12, 513
TaiI ACGT 1 cut(s) 243
TaqI TCGA 4 cut(s) 165, 186, 372, 414
TasI AATT 4 cut(s) 265, 453, 644, 673
TauI GCSGC 3 cut(s) 312, 315, 521
Tru1I TTAA 2 cut(s) 500, 588
Tru9I TTAA 2 cut(s) 500, 588
TseI GCWGC 4 cut(s) 143, 246, 521, 524
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 271
VspI ATTAAT 1 cut(s) 500
XhoI CTCGAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.