Rh2CG241300

Haloacid dehalogenase-like hydrolase domain-containing protein At4g39970

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007382
Physical Location & Seq
Forward (+)
25101609 .. 25120742
19134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG241300.1

Sequence Viewer

Length: 702 bp
ATGGCGTTCACCATGGCTCTCTCTTCCCCCTCCTCCTCTTTTCTACTCTCCCACACCACCCGCCTTCACTTCCGAAGCCCCAGAAACCTCTCCACCCCCACACGACGACCTCTTTCACTCTCAGTCTCAGCTTCTTCCTCTGCTGCTCTCCAAGCCTTGATTTTCGACTGCGACGGCGTCATCCTCGAGTCGGAGCACCTGCACCGCCAAGCTTACAACGACGCCTTCGCTCACTTCAACGTCCGCTGCTCCTCTCAGACCCTCAATTGGGACCCCGAGTTCTACGACCAGCTCCAGAACCAAATCGGCGGCGGCAAGCCCAAAATGCGATGGTACTTTAAGGAGAACGGGTGGCCGTCGTCCACCATTTTCGAGAAGCCTCCAGAAGACGATGAGGGCCGAGCCAAGATCATCGACACTCTTCAGGATTGGAAAACTGAGAGGTACAAAGAAATTATCAAGTCCGGAACTGTGGAACCAAGACCAGGAGTTTTGAGATTAATGGATGAGGCCAAGGCCGCTGGGAAAAAGCTCGCTGTTTGCTCTGCAGCTACTAAGAGTTCAGTTATATTATGTCTTGAAAGCCTTATAGGAATTGAGCGGTTTCAAGGTCTTGATTGCTTTCTTGCTGAGAAGTCGTCGGTGACTGGAAGTTGCCGATTAAGTAGTTTGATCGAAATTCTCAGATCCATGAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2

Protein Analysis

233

Amino Acids

26.05

Weight (kDa)

8.51

Isoelectric Point (pI)

59.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 51 - 202 2.7e-11 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 54 - 202 2.9e-07 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 207
AccBSI CCGCTC 1 cut(s) 601
AccIII TCCGGA 1 cut(s) 464
AciI CCGC 7 cut(s) 61, 205, 244, 309, 312, 519, 601
AclWI GGATC 1 cut(s) 681
AcoI YGGCCR 1 cut(s) 353
AcsI RAATTY 1 cut(s) 678
AcuI CTGAAG 1 cut(s) 407
AcyI GRCGYC 2 cut(s) 177, 222
AfaI GTAC 2 cut(s) 335, 446
AfiI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
AgsI TTSAA 3 cut(s) 238, 581, 608
AjnI CCWGG 1 cut(s) 484
AloI GAACNNNNNNTCC 2 cut(s) 263, 295
AluBI AGCT 5 cut(s) 131, 212, 292, 532, 551
AluI AGCT 5 cut(s) 131, 212, 292, 532, 551
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 1 cut(s) 681
Ama87I CYCGRG 2 cut(s) 185, 275
Aor13HI TCCGGA 1 cut(s) 464
AoxI GGCC 4 cut(s) 353, 397, 510, 516
ApeKI GCWGC 3 cut(s) 143, 246, 548
ApoI RAATTY 1 cut(s) 678
AseI ATTAAT 1 cut(s) 500
AspS9I GGNCC 2 cut(s) 271, 397
AsuHPI GGTGA 1 cut(s) 655
AvaI CYCGRG 2 cut(s) 185, 275
AvaII GGWCC 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 130, 233, 560
BccI CCATC 1 cut(s) 324
BceAI ACGGC 2 cut(s) 190, 340
BciT130I CCWGG 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 130
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 207
BisI GCNGC 6 cut(s) 144, 247, 310, 313, 519, 549
BlsI GCNGC 6 cut(s) 145, 248, 311, 314, 520, 550
Bme1390I CCNGG 1 cut(s) 486
Bme18I GGWCC 1 cut(s) 271
BmeT110I CYCGRG 2 cut(s) 185, 275
BmgT120I GGNCC 2 cut(s) 271, 397
BmiI GGNNCC 3 cut(s) 272, 273, 477
BmrFI CCNGG 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 2 cut(s) 278, 366
BsaHI GRCGYC 2 cut(s) 177, 222
BsaJI CCNNGG 2 cut(s) 12, 513
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
Bse1I ACTGG 1 cut(s) 652
BseAI TCCGGA 1 cut(s) 464
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 12, 513
BseGI GGATG 2 cut(s) 180, 511
BseLI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
BseMII CTCAG 6 cut(s) 135, 141, 269, 429, 621, 697
BseNI ACTGG 1 cut(s) 652
BseRI GAGGAG 3 cut(s) 22, 25, 241
BseXI GCAGC 3 cut(s) 130, 233, 560
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 1 cut(s) 185
BshFI GGCC 4 cut(s) 355, 399, 512, 518
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 2 cut(s) 185, 275
BsiSI CCGG 1 cut(s) 465
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 4 cut(s) 190, 267, 268, 485
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 4 cut(s) 355, 399, 512, 518
BsoBI CYCGRG 2 cut(s) 185, 275
Bsp1286I GDGCHC 1 cut(s) 198
Bsp13I TCCGGA 1 cut(s) 464
Bsp143I GATC 3 cut(s) 408, 672, 686
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 7 cut(s) 61, 205, 244, 309, 312, 519, 601
BspANI GGCC 4 cut(s) 355, 399, 512, 518
BspCNI CTCAG 6 cut(s) 134, 140, 268, 430, 622, 696
BspEI TCCGGA 1 cut(s) 464
BspLI GGNNCC 3 cut(s) 272, 273, 477
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 1 cut(s) 681
BsrBI CCGCTC 1 cut(s) 601
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 2 cut(s) 12, 513
BssMI GATC 3 cut(s) 408, 672, 686
BssNI GRCGYC 2 cut(s) 177, 222
BssT1I CCWWGG 2 cut(s) 12, 513
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 2 cut(s) 28, 426
BstACI GRCGYC 2 cut(s) 177, 222
BstC8I GCNNGC 2 cut(s) 317, 534
BstDEI CTNAG 7 cut(s) 121, 127, 255, 438, 555, 630, 683
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 180, 511
BstKTI GATC 3 cut(s) 411, 675, 689
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 3 cut(s) 408, 672, 686
BstMWI GCNNNNNNNGC 3 cut(s) 152, 325, 518
BstNI CCWGG 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 484
BstSFI CTRYAG 1 cut(s) 546
BstV1I GCAGC 3 cut(s) 130, 233, 560
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 686
BstYI RGATCY 1 cut(s) 686
BsuRI GGCC 4 cut(s) 355, 399, 512, 518
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 343
BtsCI GGATG 2 cut(s) 180, 511
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 317, 534
Cfr13I GGNCC 2 cut(s) 271, 397
CseI GACGC 2 cut(s) 166, 230
Csp6I GTAC 2 cut(s) 334, 445
CviAII CATG 2 cut(s) 13, 691
CviQI GTAC 2 cut(s) 334, 445
DdeI CTNAG 7 cut(s) 121, 127, 255, 438, 555, 630, 683
DpnI GATC 3 cut(s) 410, 674, 688
DpnII GATC 3 cut(s) 408, 672, 686
EaeI YGGCCR 1 cut(s) 353
Eam1104I CTCTTC 2 cut(s) 28, 426
EarI CTCTTC 2 cut(s) 28, 426
Eco130I CCWWGG 2 cut(s) 12, 513
Eco47I GGWCC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 407
Eco88I CYCGRG 2 cut(s) 185, 275
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 2 cut(s) 12, 513
ErhI CCWWGG 2 cut(s) 12, 513
FaeI CATG 2 cut(s) 16, 694
FaiI YATR 5 cut(s) 14, 569, 574, 590, 692
FaqI GGGAC 1 cut(s) 284
FatI CATG 2 cut(s) 12, 690
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 6 cut(s) 144, 247, 310, 313, 519, 549
FokI GGATG 2 cut(s) 167, 518
Fsp4HI GCNGC 6 cut(s) 144, 247, 310, 313, 519, 549
GluI GCNGC 6 cut(s) 144, 247, 310, 313, 519, 549
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 2 cut(s) 278, 366
HaeIII GGCC 4 cut(s) 355, 399, 512, 518
HapII CCGG 1 cut(s) 465
HgaI GACGC 2 cut(s) 166, 230
Hin1I GRCGYC 2 cut(s) 177, 222
Hin1II CATG 2 cut(s) 16, 694
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 465
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 2 cut(s) 9, 363
Hpy188I TCNGA 4 cut(s) 74, 193, 258, 686
Hpy188III TCNNGA 7 cut(s) 295, 373, 383, 425, 465, 578, 614
Hpy8I GTNNAC 2 cut(s) 9, 363
Hpy99I CGWCG 5 cut(s) 108, 176, 224, 361, 643
HpyAV CCTTC 2 cut(s) 74, 235
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 202, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 325, 518
HpyF3I CTNAG 7 cut(s) 121, 127, 255, 438, 555, 630, 683
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 2 cut(s) 177, 222
Hsp92II CATG 2 cut(s) 16, 694
KflI GGGWCCC 1 cut(s) 271
Kpn2I TCCGGA 1 cut(s) 464
Kzo9I GATC 3 cut(s) 408, 672, 686
LmnI GCTCC 3 cut(s) 193, 254, 297
Lsp1109I GCAGC 3 cut(s) 130, 233, 560
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 643
MalI GATC 3 cut(s) 410, 674, 688
MbiI CCGCTC 1 cut(s) 601
MboI GATC 3 cut(s) 408, 672, 686
MboII GAAGA 4 cut(s) 15, 126, 398, 413
MfeI CAATTG 1 cut(s) 265
MflI RGATCY 1 cut(s) 686
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 4 cut(s) 265, 453, 594, 678
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 171
MroI TCCGGA 1 cut(s) 464
MseI TTAA 3 cut(s) 339, 500, 662
MspA1I CMGCKG 2 cut(s) 246, 521
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 1 cut(s) 486
MunI CAATTG 1 cut(s) 265
MvaI CCWGG 1 cut(s) 486
MwoI GCNNNNNNNGC 3 cut(s) 152, 325, 518
NcoI CCATGG 1 cut(s) 12
NdeII GATC 3 cut(s) 408, 672, 686
NlaIII CATG 2 cut(s) 16, 694
NlaIV GGNNCC 3 cut(s) 272, 273, 477
NmeAIII GCCGAG 1 cut(s) 425
NmuCI GTSAC 1 cut(s) 643
PaeR7I CTCGAG 1 cut(s) 185
PaqCI CACCTGC 1 cut(s) 207
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 6 cut(s) 145, 248, 311, 314, 520, 550
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 271
PshBI ATTAAT 1 cut(s) 500
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 484
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 3 cut(s) 272, 273, 477
PspPI GGNCC 2 cut(s) 271, 397
PspPPI RGGWCCY 1 cut(s) 271
PspXI VCTCGAGB 1 cut(s) 185
PstI CTGCAG 1 cut(s) 550
PsuI RGATCY 1 cut(s) 686
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 2 cut(s) 335, 446
RsaNI GTAC 2 cut(s) 334, 445
SaqAI TTAA 3 cut(s) 339, 500, 662
SatI GCNGC 6 cut(s) 144, 247, 310, 313, 519, 549
Sau3AI GATC 3 cut(s) 408, 672, 686
Sau96I GGNCC 2 cut(s) 271, 397
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 1 cut(s) 198
SfcI CTRYAG 1 cut(s) 546
Sfr274I CTCGAG 1 cut(s) 185
SinI GGWCC 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 4 cut(s) 265, 453, 594, 678
SsiI CCGC 7 cut(s) 61, 205, 244, 309, 312, 519, 601
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 2 cut(s) 12, 513
TaaI ACNGT 1 cut(s) 472
TaiI ACGT 1 cut(s) 243
TaqI TCGA 5 cut(s) 165, 186, 372, 414, 675
TasI AATT 4 cut(s) 265, 453, 594, 678
TauI GCSGC 3 cut(s) 312, 315, 521
Tru1I TTAA 3 cut(s) 339, 500, 662
Tru9I TTAA 3 cut(s) 339, 500, 662
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 3 cut(s) 143, 246, 548
Tsp45I GTSAC 1 cut(s) 643
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 271
VspI ATTAAT 1 cut(s) 500
XapI RAATTY 1 cut(s) 678
XhoI CTCGAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.