Rh2BG452700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009036
Physical Location & Seq
Reverse (-)
63854116 .. 63857633
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG452700.1

Sequence Viewer

Length: 339 bp
ATGGCCTCCCAAGCCGGAACCATGATGCTGGATCTCAATTCGTTCTCCGACGAGAACTTCGACTGGAAGAACGACTTGACGGTGAACTCGAGGCTCTGGCAGGGCGGAGGCGGCGACGTACTAGCAACTTGTACCGGCCGAAAACGCCAACTGCCGGTTCCAGATCAAGATAGAGAAGCCGATCTACGACACGGTCGACGAGGACGAATACGACTCGATCATCGCCAAGCGCCGCGAGGAGTTCGGGGACTTCATCGAGGACGACGACGGCCTAGGGTACTGCGACAACGGCGAGGAAGAGGACTGGACCAGCCGCGGGTTTCCAATGTCATCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.81

Weight (kDa)

11.76

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 196
AccII CGCG 2 cut(s) 235, 316
AciI CCGC 5 cut(s) 105, 111, 233, 314, 316
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 136
AfaI GTAC 3 cut(s) 120, 133, 279
AfiI CCNNNNNNNGG 2 cut(s) 154, 316
AjuI GAANNNNNNNTTGG 2 cut(s) 141, 173
AlwI GGATC 1 cut(s) 39
Ama87I CYCGRG 1 cut(s) 88
AoxI GGCC 3 cut(s) 3, 136, 269
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AspA2I CCTAGG 1 cut(s) 272
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 307
AsuHPI GGTGA 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 88
AvaII GGWCC 1 cut(s) 307
AvrII CCTAGG 1 cut(s) 272
BarI GAAGNNNNNNTAC 2 cut(s) 168, 200
BceAI ACGGC 2 cut(s) 284, 305
BfaI CTAG 2 cut(s) 122, 273
BfoI RGCGCY 1 cut(s) 233
BisI GCNGC 3 cut(s) 112, 233, 314
BlnI CCTAGG 1 cut(s) 272
BlsI GCNGC 3 cut(s) 113, 234, 315
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 2 cut(s) 19, 159
BmsI GCATC 1 cut(s) 15
BsaJI CCNNGG 2 cut(s) 272, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 316
Bse118I RCCGGY 2 cut(s) 134, 154
Bse1I ACTGG 2 cut(s) 68, 309
BseDI CCNNGG 2 cut(s) 272, 314
BseLI CCNNNNNNNGG 2 cut(s) 154, 316
BseNI ACTGG 2 cut(s) 68, 309
BseRI GAGGAG 1 cut(s) 252
BseX3I CGGCCG 1 cut(s) 136
Bsh1236I CGCG 2 cut(s) 235, 316
Bsh1285I CGRYCG 2 cut(s) 139, 196
BshFI GGCC 3 cut(s) 5, 138, 271
BsiEI CGRYCG 2 cut(s) 139, 196
BsiHKCI CYCGRG 1 cut(s) 88
BsiSI CCGG 3 cut(s) 15, 135, 155
BslFI GGGAC 1 cut(s) 261
BslI CCNNNNNNNGG 2 cut(s) 154, 316
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 3 cut(s) 5, 138, 271
BsoBI CYCGRG 1 cut(s) 88
Bsp143I GATC 4 cut(s) 31, 163, 181, 217
BspACI CCGC 5 cut(s) 105, 111, 233, 314, 316
BspANI GGCC 3 cut(s) 5, 138, 271
BspFNI CGCG 2 cut(s) 235, 316
BspLI GGNNCC 2 cut(s) 19, 159
BspPI GGATC 1 cut(s) 39
BsrFI RCCGGY 2 cut(s) 134, 154
BsrI ACTGG 2 cut(s) 68, 309
BssAI RCCGGY 2 cut(s) 134, 154
BssECI CCNNGG 2 cut(s) 272, 314
BssMI GATC 4 cut(s) 31, 163, 181, 217
BssT1I CCWWGG 1 cut(s) 272
Bst4CI ACNGT 2 cut(s) 82, 194
Bst6I CTCTTC 1 cut(s) 292
BstDSI CCRYGG 1 cut(s) 314
BstFNI CGCG 2 cut(s) 235, 316
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 4 cut(s) 34, 166, 184, 220
BstMBI GATC 4 cut(s) 31, 163, 181, 217
BstMCI CGRYCG 2 cut(s) 139, 196
BstMWI GCNNNNNNNGC 4 cut(s) 11, 111, 144, 289
BstUI CGCG 2 cut(s) 235, 316
BstX2I RGATCY 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 28
BstYI RGATCY 1 cut(s) 31
BstZI CGGCCG 1 cut(s) 136
BsuRI GGCC 3 cut(s) 5, 138, 271
BtgI CCRYGG 1 cut(s) 314
BtgZI GCGATG 1 cut(s) 206
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 2 cut(s) 134, 154
Cfr13I GGNCC 1 cut(s) 307
Cfr42I CCGCGG 1 cut(s) 317
Csp6I GTAC 3 cut(s) 119, 132, 278
CviAII CATG 1 cut(s) 22
CviJI RGCY 7 cut(s) 5, 14, 94, 138, 179, 271, 313
CviKI_1 RGCY 7 cut(s) 5, 14, 94, 138, 179, 271, 313
CviQI GTAC 3 cut(s) 119, 132, 278
DpnI GATC 4 cut(s) 33, 165, 183, 219
DpnII GATC 4 cut(s) 31, 163, 181, 217
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
EciI GGCGGA 1 cut(s) 120
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 307
Eco52I CGGCCG 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 272
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 1 cut(s) 25
FaiI YATR 1 cut(s) 23
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 1 cut(s) 261
FatI CATG 1 cut(s) 21
FauI CCCGC 1 cut(s) 309
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 3 cut(s) 112, 233, 314
Fsp4HI GCNGC 3 cut(s) 112, 233, 314
FspBI CTAG 2 cut(s) 122, 273
GlaI GCGC 1 cut(s) 231
GluI GCNGC 3 cut(s) 112, 233, 314
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 3 cut(s) 5, 138, 271
HapII CCGG 3 cut(s) 15, 135, 155
HhaI GCGC 1 cut(s) 232
Hin1II CATG 1 cut(s) 25
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 1 cut(s) 197
HindII GTYRAC 1 cut(s) 197
HinfI GANTC 1 cut(s) 213
HpaII CCGG 3 cut(s) 15, 135, 155
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 85, 197
Hpy188I TCNGA 2 cut(s) 49, 335
Hpy188III TCNNGA 2 cut(s) 161, 167
Hpy8I GTNNAC 2 cut(s) 85, 197
Hpy99I CGWCG 5 cut(s) 53, 119, 201, 267, 270
HpyCH4III ACNGT 2 cut(s) 82, 194
HpyCH4IV ACGT 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 111, 144, 289
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 1 cut(s) 25
HspAI GCGC 1 cut(s) 230
KspI CCGCGG 1 cut(s) 317
Kzo9I GATC 4 cut(s) 31, 163, 181, 217
LweI GCATC 1 cut(s) 15
MaeI CTAG 2 cut(s) 122, 273
MaeII ACGT 1 cut(s) 117
MalI GATC 4 cut(s) 33, 165, 183, 219
MboI GATC 4 cut(s) 31, 163, 181, 217
MboII GAAGA 2 cut(s) 79, 309
MflI RGATCY 1 cut(s) 31
MluCI AATT 1 cut(s) 37
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 8 cut(s) 16, 84, 101, 194, 230, 251, 287, 293
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 3 cut(s) 15, 135, 155
MvnI CGCG 2 cut(s) 235, 316
MwoI GCNNNNNNNGC 4 cut(s) 11, 111, 144, 289
NdeII GATC 4 cut(s) 31, 163, 181, 217
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 2 cut(s) 19, 159
PaeR7I CTCGAG 1 cut(s) 88
PcsI WCGNNNNNNNCGW 5 cut(s) 57, 86, 193, 202, 262
PflFI GACNNNGTC 1 cut(s) 192
PkrI GCNGC 3 cut(s) 113, 234, 315
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 19, 159
PspPI GGNCC 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 88
PsuI RGATCY 1 cut(s) 31
PsyI GACNNNGTC 1 cut(s) 192
RsaI GTAC 3 cut(s) 120, 133, 279
RsaNI GTAC 3 cut(s) 119, 132, 278
SacII CCGCGG 1 cut(s) 317
SalI GTCGAC 1 cut(s) 195
SatI GCNGC 3 cut(s) 112, 233, 314
Sau3AI GATC 4 cut(s) 31, 163, 181, 217
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 1 cut(s) 207
SetI ASST 1 cut(s) 120
SfaNI GCATC 1 cut(s) 15
Sfr274I CTCGAG 1 cut(s) 88
Sfr303I CCGCGG 1 cut(s) 317
SgrBI CCGCGG 1 cut(s) 317
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
Sse9I AATT 1 cut(s) 37
SsiI CCGC 5 cut(s) 105, 111, 233, 314, 316
SspMI CTAG 2 cut(s) 122, 273
StyI CCWWGG 1 cut(s) 272
TaaI ACNGT 2 cut(s) 82, 194
TaiI ACGT 1 cut(s) 120
TaqI TCGA 5 cut(s) 60, 89, 196, 216, 256
TasI AATT 1 cut(s) 37
TauI GCSGC 3 cut(s) 114, 235, 316
TspDTI ATGAA 1 cut(s) 242
Tth111I GACNNNGTC 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 307
XhoI CTCGAG 1 cut(s) 88
XmaJI CCTAGG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 196
XspI CTAG 2 cut(s) 122, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.