Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0009145

Physical Location & Seq

Genomic Coordinates Forward (+)

75679371 .. 75681787

Gene Structure 2417 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG577700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGAGATGGTCAGCACACACTGCCTTGACGTTACTCTCCACGCGCCACCTCCGTTCTCTCATCCGAGATTGCACGCGCCGATCCTCCATCGAAGCGGGCAAGAAGCTCCACGCGGCCATCATCACCAGCGGGTTCGCCGCCTCGGCAGACTCTTTCCTCTACAATGCCCTGCTCCATCTCCACGCAGCCTGTGGCAGTGCAGTTCATGCACGCAAGGTGTTCGACGAAATTCCCAAGTCGCACAAAGACACTGTGGACTGGACCGTCCTCATGGGAAGCTTTGCTCGCCACGGCATGCATCAAACTGGGCTTCGTTTGTTTGTGGAAATGAGAAGAGAGGGAGTGAGGGTGGACGACGTTGCCATGGTTTGCTTGTTCAGTGGGTGTGCTCGGTTGGGCAACGTTGAGGTTGGGTTGCAAGGACATGGGTTCGTGGTGAAGGTGGGGTTGAGTTCTAGTGTCAGGTCGTGCAATGCGGCTATGGATATGTATGTCAAGTGTGGCCAGTTGAGTATGGCGAAGAGGGTTTTCGACGAAATGGTGGAGCGGAGTGTTGTGTCTTGGACTGTGATTTTGGATGGGGTGGTTAGATGGGAAGGTGTGGGAAGTGGGAGAGTGGCGTTTGATATAATGCCTGAGAGGAATGAGGTTGCTTGGACGATCATGATTGTTGGGTATGTGAGTGTTGGGCTTGTCCGGGAAGGTTTCTCTCTTCTGCAAGAAATGGTTTTTGGTTGTGGTTTGGGATTGAACTATGTCACACTTTGTTCGTTTTTATCGGCGTGTACACAATCAGGAGATATGGTGACGGGCAGTTGGGTTCATGCCTATGCTTATAAGACAATGGGGAATGAGATGGATATTATGGTGGGCACAGCTCTTGTTGACATGTATGCAAAATGTGGTAGAGTTGACACAGCGTTGAAAGTTTTTGAGCACATGAAGTACAGGAATGTAGTGACATGGAATGCTTTGCTTAGTGGGTTGGCAATGCATGGAAGAGGCAGAGTTGTTTTGAAGGTGTTTCCTCAAATGCTAAAAGAGGCCAAGCCAGACGATTTAACCTTCACTGCTTTGTTGTGTGCTTGCAGCCACTCAGGACTTGTTGAACAGGGTCGGCATTACTTTCGGAATCTTGAAGCATTGTATGGCGTAACACCTAAGATTGAGCATTATGCTTGCATGGTGGATCTTCTGGGCAGGGCTGGCCATTTAGAAGAGGCTGGGATTTTAATCAAGAGAATGCCAATGCCTCCGAATGAGGTTGTTTTGGGTTCGCTTATTGGTTCTTGCAGTGTCCATGGAAAACTACAGCTAGGTGAAGGCATTCTGCAAGACCTGGTTCAAATAGATCCCCAAAACACAGAGTATCATGTCCTTCTCTCAAACATGTATGCTTTGGGTGGAAACCAGGACAAGGCTAATTTGTTGAGGCAGGGCCTTAAGAATAGGGGTATAAGAAAGATGCCTGGAATGAGCTCGCTTTATGTCGGTGGCCAAATTCATCAGTTCAGTGCTGGAGACAAGTCACACCCAAAGGCAATGGAGATTTACATGAAGTTGGATGAGATGATACAAAAGTTGAGAATGGCCGGTTATGTCCCAAATGCTGCTACTCAAGTTTTCTCTGGTTTTGACAACACAGACGAGAGTAATGTGGATAAGTTGGAGGAGATAGAACAGGCATTGTTCTCCCACAGTGAAAAGTTGGCAGTTTGTTATGGACTTATAAGCGCAAGAGCAGGTTCGCCGCTTCACATATTCAAAAATCTACGCATCTGCCAAGACTGTCATTCTGCGATGAAACTAGTTTCTAATGTTTATAATCGAGAAATTGTCATCAGGGATCGCAACCGATTTCATTGTTTCAAGCAAGGTTCTTGTTCTTGCTCTGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

634

Amino Acids

70.26

Weight (kDa)

8.56

Isoelectric Point (pI)

43.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	83 - 131	4.3e-07	PPR repeat family
PPR_2	PF13041	317 - 364	1.3e-08	PPR repeat family
E_motif	PF20431	435 - 494	1.7e-09	E motif
Eplus_motif	PF20430	501 - 528	6.6e-09	E+ motif
DYW_deaminase	PF14432	557 - 634	9e-33	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009145)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15340
fragaria_vesca	FvH4_6g47920
malus_domestica	MD09G1055200.v1.1 MD09G1055600.v1.1 MD17G1053300.v1.1
prunus_persica	Prupe.3G263400_v2.0.a1
pyrus_communis	pycom111g04650 pycom17g05110
rosa_chinensis	RchiOBHm_Chr2g0167121 RchiOBHm_Chr2g0167141
rosa_laevigata	RLG00000021698
rosa_multiflora	Rmu_sc0016102.1_g000002 Rmu_sc0033453.1_g000001
rosa_roxburghii	Rroxscaffold_2G00083680 Rroxscaffold_2G00083700
rosa_rugosa	Rorug02G0528600 Rorug02G0528700 Rorug02G0528900.1
rosa_samantha	Rh2BG606700 Rh2CG577700 Rh2DG618400
rosa_wichuraiana	Rw2G049590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	837, 1730, 1824
AasI	GACNNNNNNGTC	1 cut(s)	263
Acc36I	ACCTGC	1 cut(s)	1733
AccBSI	CCGCTC	1 cut(s)	547
AccII	CGCG	3 cut(s)	43, 76, 113
AciI	CCGC	7 cut(s)	95, 113, 129, 138, 476, 547, 1751
AclI	AACGTT	1 cut(s)	402
AclWI	GGATC	4 cut(s)	75, 1197, 1346, 1854
AcoI	YGGCCR	5 cut(s)	114, 502, 1207, 1495, 1590
AcsI	RAATTY	2 cut(s)	228, 1500
AfaI	GTAC	2 cut(s)	787, 947
AfiI	CCNNNNNNNGG	1 cut(s)	1417
AflII	CTTAAG	1 cut(s)	1442
AflIII	ACRYGT	2 cut(s)	888, 1389
AgsI	TTSAA	8 cut(s)	751, 925, 1018, 1109, 1139, 1346, 1765, 1870
AhlI	ACTAGT	1 cut(s)	1807
AjnI	CCWGG	3 cut(s)	1338, 1410, 1468
AjuI	GAANNNNNNNTTGG	2 cut(s)	714, 746
AluBI	AGCT	5 cut(s)	106, 279, 878, 1315, 1479
AluI	AGCT	5 cut(s)	106, 279, 878, 1315, 1479
Alw21I	GWGCWC	3 cut(s)	391, 939, 1481
Alw26I	GTCTC	1 cut(s)	1515
AlwI	GGATC	4 cut(s)	75, 1197, 1346, 1854
AoxI	GGCC	7 cut(s)	114, 502, 1044, 1207, 1438, 1495, 1590
ApeKI	GCWGC	3 cut(s)	185, 1089, 1610
ApoI	RAATTY	2 cut(s)	228, 1500
ArsI	GACNNNNNNTTYG	2 cut(s)	556, 588
Asp700I	GAANNNNTTC	1 cut(s)	1326
AspLEI	GCGC	3 cut(s)	45, 78, 1736
AspS9I	GGNCC	2 cut(s)	261, 1438
AsuC2I	CCSGG	1 cut(s)	698
AsuHPI	GGTGA	4 cut(s)	115, 448, 817, 1331
AvaII	GGWCC	1 cut(s)	261
BaeGI	GKGCMC	1 cut(s)	875
BaeI	ACNNNNGTAYC	2 cut(s)	1565, 1598
BalI	TGGCCA	3 cut(s)	504, 1209, 1497
BanII	GRGCYC	1 cut(s)	1481
Bbv12I	GWGCWC	3 cut(s)	391, 939, 1481
BbvI	GCAGC	3 cut(s)	197, 1101, 1597
BccI	CCATC	6 cut(s)	95, 125, 183, 572, 585, 850
BceAI	ACGGC	1 cut(s)	307
BcgI	CGANNNNNNTGC	4 cut(s)	202, 236, 1109, 1143
BciT130I	CCWGG	3 cut(s)	1340, 1412, 1470
BcnI	CCSGG	1 cut(s)	698
BcoDI	GTCTC	1 cut(s)	1515
BcuI	ACTAGT	1 cut(s)	1807
BfaI	CTAG	3 cut(s)	456, 1316, 1808
BfmI	CTRYAG	1 cut(s)	1310
BfrI	CTTAAG	1 cut(s)	1442
BfuAI	ACCTGC	1 cut(s)	1733
BglI	GCCNNNNNGGC	1 cut(s)	143
BisI	GCNGC	7 cut(s)	114, 138, 186, 477, 1090, 1611, 1751
BlsI	GCNGC	7 cut(s)	115, 139, 187, 478, 1091, 1612, 1752
Bme1390I	CCNGG	4 cut(s)	698, 1340, 1412, 1470
Bme18I	GGWCC	1 cut(s)	261
BmgT120I	GGNCC	2 cut(s)	261, 1438
BmrFI	CCNGG	4 cut(s)	698, 1340, 1412, 1470
BmrI	ACTGGG	1 cut(s)	315
BmsI	GCATC	3 cut(s)	307, 1455, 1785
BmuI	ACTGGG	1 cut(s)	315
BpmI	CTGGAG	1 cut(s)	1539
BpuEI	CTTGAG	1 cut(s)	1602
BpuMI	CCSGG	1 cut(s)	698
BsaBI	GATNNNNATC	1 cut(s)	1232
BsaJI	CCNNGG	4 cut(s)	141, 289, 363, 1300
BsaXI	ACNNNNNCTCC	4 cut(s)	333, 363, 789, 819
Bsc4I	CCNNNNNNNGG	1 cut(s)	1417
Bse118I	RCCGGY	1 cut(s)	1592
Bse1I	ACTGG	3 cut(s)	263, 310, 505
Bse3DI	GCAATG	3 cut(s)	478, 996, 1548
Bse8I	GATNNNNATC	1 cut(s)	1232
BseBI	CCWGG	3 cut(s)	1340, 1412, 1470
BseDI	CCNNGG	4 cut(s)	141, 289, 363, 1300
BseGI	GGATG	3 cut(s)	60, 583, 1570
BseJI	GATNNNNATC	1 cut(s)	1232
BseLI	CCNNNNNNNGG	1 cut(s)	1417
BseMI	GCAATG	3 cut(s)	478, 996, 1548
BseMII	CTCAG	2 cut(s)	627, 1110
BseNI	ACTGG	3 cut(s)	263, 310, 505
BseRI	GAGGAG	1 cut(s)	1685
BseSI	GKGCMC	1 cut(s)	875
BseXI	GCAGC	3 cut(s)	197, 1101, 1597
BseYI	CCCAGC	1 cut(s)	1223
BsgI	GTGCAG	1 cut(s)	219
Bsh1236I	CGCG	3 cut(s)	43, 76, 113
BshFI	GGCC	7 cut(s)	116, 504, 1046, 1209, 1440, 1497, 1592
BsiHKAI	GWGCWC	3 cut(s)	391, 939, 1481
BsiSI	CCGG	2 cut(s)	697, 1593
BslFI	GGGAC	1 cut(s)	1586
BslI	CCNNNNNNNGG	1 cut(s)	1417
BsmAI	GTCTC	1 cut(s)	1515
BsmFI	GGGAC	1 cut(s)	1586
BsmI	GAATGC	3 cut(s)	973, 1248, 1326
BsnI	GGCC	7 cut(s)	116, 504, 1046, 1209, 1440, 1497, 1592
Bsp1286I	GDGCHC	4 cut(s)	391, 875, 939, 1481
Bsp1407I	TGTACA	1 cut(s)	785
Bsp143I	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
Bsp19I	CCATGG	2 cut(s)	363, 1300
BspACI	CCGC	7 cut(s)	95, 113, 129, 138, 476, 547, 1751
BspANI	GGCC	7 cut(s)	116, 504, 1046, 1209, 1440, 1497, 1592
BspCNI	CTCAG	2 cut(s)	628, 1109
BspFNI	CGCG	3 cut(s)	43, 76, 113
BspHI	TCATGA	1 cut(s)	663
BspMI	ACCTGC	1 cut(s)	1733
BspPI	GGATC	4 cut(s)	75, 1197, 1346, 1854
BspTI	CTTAAG	1 cut(s)	1442
BsrBI	CCGCTC	1 cut(s)	547
BsrDI	GCAATG	3 cut(s)	478, 996, 1548
BsrFI	RCCGGY	1 cut(s)	1592
BsrGI	TGTACA	1 cut(s)	785
BsrI	ACTGG	3 cut(s)	263, 310, 505
BssAI	RCCGGY	1 cut(s)	1592
BssECI	CCNNGG	4 cut(s)	141, 289, 363, 1300
BssMI	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
BssT1I	CCWWGG	2 cut(s)	363, 1300
Bst2UI	CCWGG	3 cut(s)	1340, 1412, 1470
Bst4CI	ACNGT	5 cut(s)	253, 265, 568, 1700, 1790
Bst6I	CTCTTC	5 cut(s)	328, 515, 717, 994, 1212
BstAFI	CTTAAG	1 cut(s)	1442
BstAPI	GCANNNNNTGC	2 cut(s)	20, 206
BstAUI	TGTACA	1 cut(s)	785
BstC8I	GCNNGC	9 cut(s)	74, 97, 211, 286, 296, 1087, 1180, 1207, 1481
BstDEI	CTNAG	4 cut(s)	636, 977, 1096, 1161
BstDSI	CCRYGG	3 cut(s)	289, 363, 1300
BstF5I	GGATG	3 cut(s)	60, 583, 1570
BstFNI	CGCG	3 cut(s)	43, 76, 113
BstHHI	GCGC	3 cut(s)	45, 78, 1736
BstKTI	GATC	5 cut(s)	83, 663, 1192, 1354, 1849
BstMAI	GTCTC	1 cut(s)	1515
BstMBI	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
BstMWI	GCNNNNNNNGC	5 cut(s)	20, 143, 206, 285, 1206
BstNI	CCWGG	3 cut(s)	1340, 1412, 1470
BstNSI	RCATGY	3 cut(s)	298, 892, 1393
BstSCI	CCNGG	4 cut(s)	696, 1338, 1410, 1468
BstSFI	CTRYAG	1 cut(s)	1310
BstSLI	GKGCMC	1 cut(s)	875
BstUI	CGCG	3 cut(s)	43, 76, 113
BstV1I	GCAGC	3 cut(s)	197, 1101, 1597
BstX2I	RGATCY	2 cut(s)	1189, 1351
BstYI	RGATCY	2 cut(s)	1189, 1351
BsuRI	GGCC	7 cut(s)	116, 504, 1046, 1209, 1440, 1497, 1592
BtgI	CCRYGG	3 cut(s)	289, 363, 1300
BtgZI	GCGATG	1 cut(s)	1814
BtsCI	GGATG	3 cut(s)	60, 583, 1570
BtsI	GCAGTG	4 cut(s)	18, 202, 1068, 1300
BtsIMutI	CAGTG	8 cut(s)	18, 202, 249, 385, 1068, 1300, 1519, 1705
BveI	ACCTGC	1 cut(s)	1733
Cac8I	GCNNGC	9 cut(s)	74, 97, 211, 286, 296, 1087, 1180, 1207, 1481
CciI	TCATGA	1 cut(s)	663
CfoI	GCGC	3 cut(s)	45, 78, 1736
Cfr10I	RCCGGY	1 cut(s)	1592
Cfr13I	GGNCC	2 cut(s)	261, 1438
CsiI	ACCWGGT	1 cut(s)	1338
Csp6I	GTAC	2 cut(s)	786, 946
CviQI	GTAC	2 cut(s)	786, 946
DdeI	CTNAG	4 cut(s)	636, 977, 1096, 1161
DpnI	GATC	5 cut(s)	82, 662, 1191, 1353, 1848
DpnII	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
DrdI	GACNNNNNNGTC	1 cut(s)	263
DseDI	GACNNNNNNGTC	1 cut(s)	263
EaeI	YGGCCR	5 cut(s)	114, 502, 1207, 1495, 1590
Eam1104I	CTCTTC	5 cut(s)	328, 515, 717, 994, 1212
EarI	CTCTTC	5 cut(s)	328, 515, 717, 994, 1212
Ecl136II	GAGCTC	1 cut(s)	1479
Eco130I	CCWWGG	2 cut(s)	363, 1300
Eco24I	GRGCYC	1 cut(s)	1481
Eco47I	GGWCC	1 cut(s)	261
Eco53kI	GAGCTC	1 cut(s)	1479
EcoICRI	GAGCTC	1 cut(s)	1479
EcoO109I	RGGNCCY	1 cut(s)	1438
EcoRII	CCWGG	3 cut(s)	1338, 1410, 1468
EcoT14I	CCWWGG	2 cut(s)	363, 1300
EcoT22I	ATGCAT	2 cut(s)	300, 996
EcoT38I	GRGCYC	1 cut(s)	1481
ErhI	CCWWGG	2 cut(s)	363, 1300
FaqI	GGGAC	1 cut(s)	1586
FauI	CCCGC	2 cut(s)	88, 122
Fnu4HI	GCNGC	7 cut(s)	114, 138, 186, 477, 1090, 1611, 1751
FokI	GGATG	3 cut(s)	47, 590, 1577
FriOI	GRGCYC	1 cut(s)	1481
Fsp4HI	GCNGC	7 cut(s)	114, 138, 186, 477, 1090, 1611, 1751
FspBI	CTAG	3 cut(s)	456, 1316, 1808
GlaI	GCGC	3 cut(s)	44, 77, 1735
GluI	GCNGC	7 cut(s)	114, 138, 186, 477, 1090, 1611, 1751
GsaI	CCCAGC	1 cut(s)	1227
GsuI	CTGGAG	1 cut(s)	1539
HaeIII	GGCC	7 cut(s)	116, 504, 1046, 1209, 1440, 1497, 1592
HapII	CCGG	2 cut(s)	697, 1593
HhaI	GCGC	3 cut(s)	45, 78, 1736
Hin6I	GCGC	3 cut(s)	43, 76, 1734
HinP1I	GCGC	3 cut(s)	43, 76, 1734
HincII	GTYRAC	2 cut(s)	886, 913
HindII	GTYRAC	2 cut(s)	886, 913
HindIII	AAGCTT	1 cut(s)	277
HinfI	GANTC	2 cut(s)	149, 1132
HpaII	CCGG	2 cut(s)	697, 1593
HphI	GGTGA	4 cut(s)	115, 448, 817, 1331
Hpy166II	GTNNAC	6 cut(s)	256, 352, 786, 788, 886, 913
Hpy188I	TCNGA	4 cut(s)	65, 1131, 1257, 1894
Hpy188III	TCNNGA	6 cut(s)	664, 795, 1098, 1136, 1237, 1829
Hpy8I	GTNNAC	6 cut(s)	256, 352, 786, 788, 886, 913
Hpy99I	CGWCG	3 cut(s)	227, 359, 536
HpyAV	CCTTC	7 cut(s)	433, 590, 695, 1012, 1075, 1316, 1388
HpyCH4III	ACNGT	5 cut(s)	253, 265, 568, 1700, 1790
HpyCH4IV	ACGT	3 cut(s)	29, 357, 402
HpyF10VI	GCNNNNNNNGC	5 cut(s)	20, 143, 206, 285, 1206
HpyF3I	CTNAG	4 cut(s)	636, 977, 1096, 1161
HpySE526I	ACGT	3 cut(s)	29, 357, 402
HspAI	GCGC	3 cut(s)	43, 76, 1734
Kzo9I	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
LmnI	GCTCC	3 cut(s)	111, 177, 544
Lsp1109I	GCAGC	3 cut(s)	197, 1101, 1597
LweI	GCATC	3 cut(s)	307, 1455, 1785
MabI	ACCWGGT	1 cut(s)	1338
MaeI	CTAG	3 cut(s)	456, 1316, 1808
MaeII	ACGT	3 cut(s)	29, 357, 402
MaeIII	GTNAC	6 cut(s)	30, 757, 805, 958, 1153, 1527
MalI	GATC	5 cut(s)	82, 662, 1191, 1353, 1848
MbiI	CCGCTC	1 cut(s)	547
MboI	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
MboII	GAAGA	6 cut(s)	345, 532, 704, 1011, 1184, 1229
MflI	RGATCY	2 cut(s)	1189, 1351
MhlI	GDGCHC	4 cut(s)	391, 875, 939, 1481
MlsI	TGGCCA	3 cut(s)	504, 1209, 1497
MluCI	AATT	4 cut(s)	228, 1423, 1500, 1833
MluNI	TGGCCA	3 cut(s)	504, 1209, 1497
MlyI	GAGTC	1 cut(s)	143
MmeI	TCCRAC	2 cut(s)	1542, 1647
Mox20I	TGGCCA	3 cut(s)	504, 1209, 1497
Mph1103I	ATGCAT	2 cut(s)	300, 996
MroXI	GAANNNNTTC	1 cut(s)	1326
MscI	TGGCCA	3 cut(s)	504, 1209, 1497
MseI	TTAA	3 cut(s)	1061, 1232, 1443
MslI	CAYNNNNRTG	1 cut(s)	828
Msp20I	TGGCCA	3 cut(s)	504, 1209, 1497
MspA1I	CMGCKG	1 cut(s)	129
MspCI	CTTAAG	1 cut(s)	1442
MspI	CCGG	2 cut(s)	697, 1593
MspR9I	CCNGG	4 cut(s)	698, 1340, 1412, 1470
Mva1269I	GAATGC	3 cut(s)	973, 1248, 1326
MvaI	CCWGG	3 cut(s)	1340, 1412, 1470
MvnI	CGCG	3 cut(s)	43, 76, 113
MwoI	GCNNNNNNNGC	5 cut(s)	20, 143, 206, 285, 1206
NciI	CCSGG	1 cut(s)	698
NcoI	CCATGG	2 cut(s)	363, 1300
NdeII	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
NmeAIII	GCCGAG	1 cut(s)	122
NmuCI	GTSAC	4 cut(s)	757, 805, 958, 1527
NsiI	ATGCAT	2 cut(s)	300, 996
NspI	RCATGY	3 cut(s)	298, 892, 1393
PaeI	GCATGC	1 cut(s)	298
PagI	TCATGA	1 cut(s)	663
PciI	ACATGT	2 cut(s)	888, 1389
PctI	GAATGC	3 cut(s)	973, 1248, 1326
PdmI	GAANNNNTTC	1 cut(s)	1326
PfeI	GAWTC	1 cut(s)	1132
PfoI	TCCNGGA	1 cut(s)	696
PkrI	GCNGC	7 cut(s)	115, 139, 187, 478, 1091, 1612, 1752
PleI	GAGTC	1 cut(s)	143
PpsI	GAGTC	1 cut(s)	143
PscI	ACATGT	2 cut(s)	888, 1389
PsiI	TTATAA	3 cut(s)	837, 1730, 1824
Psp124BI	GAGCTC	1 cut(s)	1481
Psp1406I	AACGTT	1 cut(s)	402
Psp6I	CCWGG	3 cut(s)	1338, 1410, 1468
PspFI	CCCAGC	1 cut(s)	1223
PspGI	CCWGG	3 cut(s)	1338, 1410, 1468
PspPI	GGNCC	2 cut(s)	261, 1438
PsuI	RGATCY	2 cut(s)	1189, 1351
RsaI	GTAC	2 cut(s)	787, 947
RsaNI	GTAC	2 cut(s)	786, 946
RseI	CAYNNNNRTG	1 cut(s)	828
SacI	GAGCTC	1 cut(s)	1481
SaqAI	TTAA	3 cut(s)	1061, 1232, 1443
SatI	GCNGC	7 cut(s)	114, 138, 186, 477, 1090, 1611, 1751
Sau3AI	GATC	5 cut(s)	80, 660, 1189, 1351, 1846
Sau96I	GGNCC	2 cut(s)	261, 1438
SchI	GAGTC	1 cut(s)	143
ScrFI	CCNGG	4 cut(s)	698, 1340, 1412, 1470
SduI	GDGCHC	4 cut(s)	391, 875, 939, 1481
SexAI	ACCWGGT	1 cut(s)	1338
SfaNI	GCATC	3 cut(s)	307, 1455, 1785
SfcI	CTRYAG	1 cut(s)	1310
SinI	GGWCC	1 cut(s)	261
SmiMI	CAYNNNNRTG	1 cut(s)	828
SmlI	CTYRAG	2 cut(s)	1442, 1617
SmoI	CTYRAG	2 cut(s)	1442, 1617
SpeI	ACTAGT	1 cut(s)	1807
SphI	GCATGC	1 cut(s)	298
Sse9I	AATT	4 cut(s)	228, 1423, 1500, 1833
SsiI	CCGC	7 cut(s)	95, 113, 129, 138, 476, 547, 1751
SspMI	CTAG	3 cut(s)	456, 1316, 1808
SstI	GAGCTC	1 cut(s)	1481
StyD4I	CCNGG	4 cut(s)	696, 1338, 1410, 1468
StyI	CCWWGG	2 cut(s)	363, 1300
TaaI	ACNGT	5 cut(s)	253, 265, 568, 1700, 1790
TaiI	ACGT	3 cut(s)	32, 360, 405
TaqI	TCGA	4 cut(s)	90, 222, 531, 1828
TasI	AATT	4 cut(s)	228, 1423, 1500, 1833
TatI	WGTACW	2 cut(s)	785, 945
TauI	GCSGC	4 cut(s)	116, 140, 479, 1753
TfiI	GAWTC	1 cut(s)	1132
Tru1I	TTAA	3 cut(s)	1061, 1232, 1443
Tru9I	TTAA	3 cut(s)	1061, 1232, 1443
TscAI	CASTG	8 cut(s)	25, 202, 256, 385, 1075, 1300, 1519, 1705
TseFI	GTSAC	4 cut(s)	757, 805, 958, 1527
TseI	GCWGC	3 cut(s)	185, 1089, 1610
Tsp45I	GTSAC	4 cut(s)	757, 805, 958, 1527
TspDTI	ATGAA	7 cut(s)	194, 812, 956, 1493, 1571, 1817, 1850
TspGWI	ACGGA	1 cut(s)	41
TspRI	CASTG	8 cut(s)	25, 202, 256, 385, 1075, 1300, 1519, 1705
Vha464I	CTTAAG	1 cut(s)	1442
VpaK11BI	GGWCC	1 cut(s)	261
XapI	RAATTY	2 cut(s)	228, 1500
XceI	RCATGY	3 cut(s)	298, 892, 1393
XcmI	CCANNNNNNNNNTGG	1 cut(s)	188
XmnI	GAANNNNTTC	1 cut(s)	1326
XspI	CTAG	3 cut(s)	456, 1316, 1808
Zsp2I	ATGCAT	2 cut(s)	300, 996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.