Rh3BG322000

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0015747
Physical Location & Seq
Reverse (-)
33186678 .. 33190250
3573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG322000.1

Sequence Viewer

Length: 480 bp
ATGAATGGGTCTTGGTCGTTGTTTGGGAGCAAGAATGAATCTGGTGGTGTAGACGTGTGGTTGTGGGTGAGACCTCCAGAAGCGGTTCCCTACCGGAGGACCTCGCCCGCACCGGAGCTTACTCCGATCTCTTCGTTTTCATCACTGACGGCAAAGCCGATATATTCTTCTACAGTAGCGCCTCTGATTTGTTATCCACAATCAGAAGCAAGAGACCGCGATATGAGCCACGCCAAGAGAAGCGTTCCCGGGACTGTGCCAACGAAGTCAAATTTCGACGAGCGGAAACCCTCGCCGGCTGTCACCACCTCCACCGGCAAGCGCATGATATATTTGGAAGAGTGTTGGCTTATGGACTGGGATTACTCTAATTCCTTGTCCCGTGCCATCGAAGCTTTGAGGGCTAGCACGCTCCGCCTTCTAGTGGTTGATGGAGGCAGATGTCCACAATGTGAAGGATGCTATTTCTTCCACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

159

Amino Acids

17.73

Weight (kDa)

8.3

Isoelectric Point (pI)

64.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 283
AccI GTMKAC 1 cut(s) 51
AccII CGCG 1 cut(s) 219
AciI CCGC 5 cut(s) 83, 108, 217, 283, 415
AcsI RAATTY 1 cut(s) 271
AdeI CACNNNGTG 1 cut(s) 452
AfiI CCNNNNNNNGG 2 cut(s) 96, 424
AflIII ACRYGT 1 cut(s) 54
AjiI CACGTC 1 cut(s) 55
AluBI AGCT 2 cut(s) 118, 395
AluI AGCT 2 cut(s) 118, 395
Alw26I GTCTC 2 cut(s) 64, 207
Ama87I CYCGRG 1 cut(s) 248
ApoI RAATTY 1 cut(s) 271
Asp700I GAANNNNTTC 1 cut(s) 84
AspLEI GCGC 2 cut(s) 181, 324
AspS9I GGNCC 1 cut(s) 99
AsuC2I CCSGG 2 cut(s) 249, 250
AsuHPI GGTGA 2 cut(s) 79, 295
AsuNHI GCTAGC 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 99
BccI CCATC 2 cut(s) 395, 425
BceAI ACGGC 1 cut(s) 165
BcnI CCSGG 2 cut(s) 249, 250
BcoDI GTCTC 2 cut(s) 64, 207
BfaI CTAG 2 cut(s) 405, 422
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 182
Bme1390I CCNGG 2 cut(s) 249, 250
Bme18I GGWCC 1 cut(s) 99
BmeT110I CYCGRG 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 249, 250
BmrI ACTGGG 1 cut(s) 367
BmsI GCATC 1 cut(s) 449
BmtI GCTAGC 1 cut(s) 408
BmuI ACTGGG 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 249, 250
BsaI GGTCTC 2 cut(s) 64, 207
BsaJI CCNNGG 1 cut(s) 248
BsaWI WCCGGW 2 cut(s) 93, 112
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 424
Bse118I RCCGGY 2 cut(s) 295, 314
Bse1I ACTGG 1 cut(s) 362
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 2 cut(s) 96, 424
BseNI ACTGG 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 5 cut(s) 94, 113, 249, 296, 315
BslFI GGGAC 2 cut(s) 265, 364
BslI CCNNNNNNNGG 2 cut(s) 96, 424
BsmAI GTCTC 2 cut(s) 64, 207
BsmFI GGGAC 2 cut(s) 265, 364
Bso31I GGTCTC 2 cut(s) 64, 207
BsoBI CYCGRG 1 cut(s) 248
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 5 cut(s) 83, 108, 217, 283, 415
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 87
BspOI GCTAGC 1 cut(s) 408
BspTNI GGTCTC 2 cut(s) 64, 207
BsrBI CCGCTC 1 cut(s) 283
BsrFI RCCGGY 2 cut(s) 295, 314
BsrI ACTGG 1 cut(s) 362
BssAI RCCGGY 2 cut(s) 295, 314
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 1 cut(s) 126
Bst4CI ACNGT 3 cut(s) 175, 256, 476
Bst6I CTCTTC 2 cut(s) 136, 333
BstC8I GCNNGC 5 cut(s) 108, 297, 320, 406, 410
BstENI CCTNNNNNAGG 1 cut(s) 94
BstF5I GGATG 1 cut(s) 464
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 2 cut(s) 181, 324
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 2 cut(s) 64, 207
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 4 cut(s) 225, 392, 401, 414
BstSCI CCNGG 2 cut(s) 247, 248
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 1 cut(s) 219
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 5 cut(s) 108, 297, 320, 406, 410
CfoI GCGC 2 cut(s) 181, 324
Cfr10I RCCGGY 2 cut(s) 295, 314
Cfr13I GGNCC 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 248
CviAII CATG 1 cut(s) 325
CviJI RGCY 7 cut(s) 118, 157, 228, 299, 349, 395, 404
CviKI_1 RGCY 7 cut(s) 118, 157, 228, 299, 349, 395, 404
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
DraIII CACNNNGTG 1 cut(s) 452
Eam1104I CTCTTC 2 cut(s) 136, 333
EarI CTCTTC 2 cut(s) 136, 333
EciI GGCGGA 1 cut(s) 404
Eco31I GGTCTC 2 cut(s) 64, 207
Eco47I GGWCC 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 248
EcoNI CCTNNNNNAGG 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 99
FaeI CATG 1 cut(s) 328
FaiI YATR 5 cut(s) 163, 224, 326, 331, 353
FaqI GGGAC 2 cut(s) 265, 364
FatI CATG 1 cut(s) 324
FauI CCCGC 1 cut(s) 115
FblI GTMKAC 1 cut(s) 51
FokI GGATG 1 cut(s) 471
FspBI CTAG 2 cut(s) 405, 422
GlaI GCGC 2 cut(s) 180, 323
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 182
HapII CCGG 5 cut(s) 94, 113, 249, 296, 315
HhaI GCGC 2 cut(s) 181, 324
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 2 cut(s) 179, 322
HinP1I GCGC 2 cut(s) 179, 322
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 1 cut(s) 38
HpaII CCGG 5 cut(s) 94, 113, 249, 296, 315
HphI GGTGA 2 cut(s) 79, 295
Hpy166II GTNNAC 2 cut(s) 52, 446
Hpy188I TCNGA 3 cut(s) 126, 186, 205
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 2 cut(s) 52, 446
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 2 cut(s) 428, 449
HpyCH4III ACNGT 3 cut(s) 175, 256, 476
HpyCH4IV ACGT 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 4 cut(s) 225, 392, 401, 414
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 2 cut(s) 179, 322
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 3 cut(s) 27, 115, 417
LpnPI CCDG 8 cut(s) 27, 90, 107, 126, 262, 309, 328, 343
LweI GCATC 1 cut(s) 449
MaeI CTAG 2 cut(s) 405, 422
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 1 cut(s) 301
MalI GATC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 283
MboI GATC 1 cut(s) 126
MboII GAAGA 4 cut(s) 123, 159, 350, 460
MluCI AATT 2 cut(s) 271, 370
MnlI CCTC 8 cut(s) 84, 90, 112, 192, 301, 319, 393, 428
MroNI GCCGGC 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 84
MspI CCGG 5 cut(s) 94, 113, 249, 296, 315
MspR9I CCNGG 2 cut(s) 249, 250
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 4 cut(s) 225, 392, 401, 414
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 2 cut(s) 249, 250
NdeII GATC 1 cut(s) 126
NgoMIV GCCGGC 1 cut(s) 295
NheI GCTAGC 1 cut(s) 404
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 301
PcsI WCGNNNNNNNCGW 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 1 cut(s) 38
PpuMI RGGWCCY 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 99
PspPPI RGGWCCY 1 cut(s) 99
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 249, 250
SetI ASST 6 cut(s) 57, 76, 104, 120, 311, 397
SfaNI GCATC 1 cut(s) 449
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 99
SmaI CCCGGG 1 cut(s) 250
Sse9I AATT 2 cut(s) 271, 370
SsiI CCGC 5 cut(s) 83, 108, 217, 283, 415
SspMI CTAG 2 cut(s) 405, 422
StyD4I CCNGG 2 cut(s) 247, 248
TaaI ACNGT 3 cut(s) 175, 256, 476
TaiI ACGT 1 cut(s) 57
TaqI TCGA 2 cut(s) 276, 390
TasI AATT 2 cut(s) 271, 370
TfiI GAWTC 1 cut(s) 38
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 1 cut(s) 301
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 3 cut(s) 17, 51, 129
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 99
XagI CCTNNNNNAGG 1 cut(s) 94
XapI RAATTY 1 cut(s) 271
XmaI CCCGGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 2 cut(s) 405, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.