Rh4AG046400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017869
Physical Location & Seq
Reverse (-)
9732603 .. 9732974
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG046400.1

Sequence Viewer

Length: 246 bp
ATGAGATCAAAGCTAGGAATGGAGCTCGATAGAGGGTTGATATGCTTACCACGGCTGCTACGTTGGGCTCGCATGGCTGCCGCCTTCCTACGCTGGGCAACCGATGTCCTTCAAGACGCCGATGTGGAGAAACTCAACCAGAAGGCCGAAGTGAACGGAGGAGATGTTGAGGTTGAGGGAGAGATCTCCTCTGGTCCAACGGCGCCAGAAAGTTGGTCTTGGCCTTGTTGCCGCGGAGAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

81

Amino Acids

9.02

Weight (kDa)

4.85

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 202
AccII CGCG 1 cut(s) 234
AciI CCGC 3 cut(s) 81, 232, 234
AcyI GRCGYC 2 cut(s) 117, 203
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 2 cut(s) 13, 25
AluI AGCT 2 cut(s) 13, 25
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 233
AoxI GGCC 2 cut(s) 144, 221
ApeKI GCWGC 2 cut(s) 55, 77
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 194
AvaII GGWCC 1 cut(s) 194
BanI GGYRCC 1 cut(s) 202
BanII GRGCYC 2 cut(s) 27, 70
Bbv12I GWGCWC 1 cut(s) 27
BbvI GCAGC 2 cut(s) 42, 64
BceAI ACGGC 2 cut(s) 68, 216
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 2 cut(s) 14, 244
BfoI RGCGCY 1 cut(s) 206
BglII AGATCT 1 cut(s) 183
BisI GCNGC 4 cut(s) 56, 78, 81, 232
BlsI GCNGC 4 cut(s) 57, 79, 82, 233
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BsaHI GRCGYC 2 cut(s) 117, 203
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 50, 232
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 94
BseDI CCNNGG 2 cut(s) 50, 232
BseLI CCNNNNNNNGG 1 cut(s) 94
BseRI GAGGAG 2 cut(s) 174, 178
BseXI GCAGC 2 cut(s) 42, 64
BseYI CCCAGC 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 2 cut(s) 146, 223
BshNI GGYRCC 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 233
BsnI GGCC 2 cut(s) 146, 223
Bso31I GGTCTC 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 27, 70
Bsp143I GATC 2 cut(s) 5, 183
BspACI CCGC 3 cut(s) 81, 232, 234
BspANI GGCC 2 cut(s) 146, 223
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 202
BspTNI GGTCTC 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 50, 232
BssMI GATC 2 cut(s) 5, 183
BssNI GRCGYC 2 cut(s) 117, 203
BstACI GRCGYC 2 cut(s) 117, 203
BstC8I GCNNGC 1 cut(s) 70
BstDSI CCRYGG 2 cut(s) 50, 232
BstFNI CGCG 1 cut(s) 234
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 2 cut(s) 8, 186
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 2 cut(s) 5, 183
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 42, 64
BstX2I RGATCY 1 cut(s) 183
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 2 cut(s) 146, 223
BtgI CCRYGG 2 cut(s) 50, 232
Cac8I GCNNGC 1 cut(s) 70
CfoI GCGC 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 194
Cfr42I CCGCGG 1 cut(s) 235
CseI GACGC 1 cut(s) 125
CviAII CATG 1 cut(s) 73
CviJI RGCY 7 cut(s) 13, 25, 55, 68, 77, 146, 223
CviKI_1 RGCY 7 cut(s) 13, 25, 55, 68, 77, 146, 223
DinI GGCGCC 1 cut(s) 204
DpnI GATC 2 cut(s) 7, 185
DpnII GATC 2 cut(s) 5, 183
Ecl136II GAGCTC 1 cut(s) 25
Eco24I GRGCYC 2 cut(s) 27, 70
Eco31I GGTCTC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoT38I GRGCYC 2 cut(s) 27, 70
EgeI GGCGCC 1 cut(s) 204
EheI GGCGCC 1 cut(s) 204
FaeI CATG 1 cut(s) 76
FaiI YATR 2 cut(s) 43, 74
FalI AAGNNNNNCTT 2 cut(s) 202, 234
FatI CATG 1 cut(s) 72
Fnu4HI GCNGC 4 cut(s) 56, 78, 81, 232
FriOI GRGCYC 2 cut(s) 27, 70
Fsp4HI GCNGC 4 cut(s) 56, 78, 81, 232
FspBI CTAG 2 cut(s) 14, 244
GlaI GCGC 1 cut(s) 204
GluI GCNGC 4 cut(s) 56, 78, 81, 232
GsaI CCCAGC 1 cut(s) 97
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 2 cut(s) 146, 223
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 205
Hin1I GRCGYC 2 cut(s) 117, 203
Hin1II CATG 1 cut(s) 76
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
Hpy166II GTNNAC 1 cut(s) 154
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 3 cut(s) 94, 119, 136
HpyCH4IV ACGT 1 cut(s) 61
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpySE526I ACGT 1 cut(s) 61
Hsp92I GRCGYC 2 cut(s) 117, 203
Hsp92II CATG 1 cut(s) 76
HspAI GCGC 1 cut(s) 203
KasI GGCGCC 1 cut(s) 202
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 2 cut(s) 5, 183
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 4 cut(s) 79, 152, 177, 219
Lsp1109I GCAGC 2 cut(s) 42, 64
MaeI CTAG 2 cut(s) 14, 244
MaeII ACGT 1 cut(s) 61
MalI GATC 2 cut(s) 7, 185
MboI GATC 2 cut(s) 5, 183
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 2 cut(s) 27, 70
Mly113I GGCGCC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 221
MnlI CCTC 5 cut(s) 26, 152, 163, 169, 199
MspA1I CMGCKG 1 cut(s) 234
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 74
NarI GGCGCC 1 cut(s) 203
NdeII GATC 2 cut(s) 5, 183
NlaIII CATG 1 cut(s) 76
NlaIV GGNNCC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PkrI GCNGC 4 cut(s) 57, 79, 82, 233
PluTI GGCGCC 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 194
PsuI RGATCY 1 cut(s) 183
SacI GAGCTC 1 cut(s) 27
SacII CCGCGG 1 cut(s) 235
SatI GCNGC 4 cut(s) 56, 78, 81, 232
Sau3AI GATC 2 cut(s) 5, 183
Sau96I GGNCC 1 cut(s) 194
SduI GDGCHC 2 cut(s) 27, 70
SetI ASST 5 cut(s) 15, 27, 64, 174, 245
SfoI GGCGCC 1 cut(s) 204
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SinI GGWCC 1 cut(s) 194
SsiI CCGC 3 cut(s) 81, 232, 234
SspDI GGCGCC 1 cut(s) 202
SspMI CTAG 2 cut(s) 14, 244
SstI GAGCTC 1 cut(s) 27
TaiI ACGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 27
TauI GCSGC 2 cut(s) 83, 234
TseI GCWGC 2 cut(s) 55, 77
TspGWI ACGGA 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 194
XspI CTAG 2 cut(s) 14, 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.