Rh4AG049500

Protein phosphatase 2C-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007891
Physical Location & Seq
Reverse (-)
10386243 .. 10389536
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG049500.1

Sequence Viewer

Length: 576 bp
ATGTTTGGAAGATGGAGAAACCTAGAAACAAAGCAAGGCTTTCAGCTGAGGCGAATTCTCAAGATAAAAACTGGGGTTAAGAAGAAAGAGGCTAATTTTACTACAAAACCTTCATGGATGATGCCAGCAGTCTCACATGGGCATCATGTGGTTGAGGGTTATGATGAATTCGACTGTGACTATGTTGTGGCTCAGAGAGAGCAGATTGAAGAGCTTGAGGTGTGGCTTTTCGGAGTTTCCGATCCTCGGATTGGGGGCGGAATTACCAAGTACATGCAGGCACATCTGTTTGATAAGAAGCCAAAAGAGTCTATCATAAAGGGAAAGAGCAACGGGACGATGAGAAAAGCATATCTTGATGCTAAAGCAAAGCTTAGAGACGCCCAGGGAACAGAGGAGACAAATGGAGTCGGTTCAGTTTCGGCAATGGTGATCAATGGTGAAAAGCTAGTATTAGCGAATATCGGAGACTACAGAGCAATTGTTTGCAGAGAGGGTTTGGCTCATGAAATAGGCATTAGGCACAAGCAATCACCCAAAATGAGTTGGACACATAGACTCTTTAAAGGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 map04011
Pfam Domains
Protein Families
PP2C

Protein Analysis

191

Amino Acids

21.69

Weight (kDa)

9.62

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 76 - 176 2.2e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 258
AclWI GGATC 1 cut(s) 236
AcsI RAATTY 2 cut(s) 54, 167
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 246, 251
AgsI TTSAA 1 cut(s) 209
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 4 cut(s) 46, 214, 373, 448
AluI AGCT 4 cut(s) 46, 214, 373, 448
Alw26I GTCTC 4 cut(s) 136, 372, 392, 462
AlwI GGATC 1 cut(s) 236
ApoI RAATTY 2 cut(s) 54, 167
AsuHPI GGTGA 3 cut(s) 442, 452, 525
BbvCI CCTCAGC 1 cut(s) 47
BccI CCATC 1 cut(s) 6
BciT130I CCWGG 1 cut(s) 386
BclI TGATCA 1 cut(s) 432
BcoDI GTCTC 4 cut(s) 136, 372, 392, 462
BfaI CTAG 2 cut(s) 23, 449
BfmI CTRYAG 1 cut(s) 472
Bme1390I CCNGG 1 cut(s) 386
BmrFI CCNGG 1 cut(s) 386
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 3 cut(s) 111, 151, 349
BmuI ACTGGG 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 47
BpuEI CTTGAG 2 cut(s) 44, 236
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 3 cut(s) 245, 384, 385
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 251
Bse1I ACTGG 1 cut(s) 76
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 245, 384, 385
BseGI GGATG 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 246, 251
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 2 cut(s) 38, 206
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 410
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 2 cut(s) 246, 251
BsmAI GTCTC 4 cut(s) 136, 372, 392, 462
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 349
Bsp143I GATC 2 cut(s) 241, 432
BspACI CCGC 1 cut(s) 258
BspCNI CTCAG 2 cut(s) 39, 205
BspHI TCATGA 1 cut(s) 505
BspPI GGATC 1 cut(s) 236
BspQI GCTCTTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 245, 384, 385
BssMI GATC 2 cut(s) 241, 432
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 204
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 2 cut(s) 126, 279
BstDEI CTNAG 3 cut(s) 47, 192, 374
BstF5I GGATG 1 cut(s) 123
BstKTI GATC 2 cut(s) 244, 435
BstMAI GTCTC 4 cut(s) 136, 372, 392, 462
BstMBI GATC 2 cut(s) 241, 432
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 472
BtsCI GGATG 1 cut(s) 123
Cac8I GCNNGC 2 cut(s) 126, 279
CciI TCATGA 1 cut(s) 505
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 271
CviAII CATG 5 cut(s) 114, 137, 146, 274, 506
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 3 cut(s) 47, 192, 374
DpnI GATC 2 cut(s) 243, 434
DpnII GATC 2 cut(s) 241, 432
DraI TTTAAA 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EciI GGCGGA 1 cut(s) 273
EcoRI GAATTC 2 cut(s) 54, 167
EcoRII CCWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 5 cut(s) 117, 140, 149, 277, 509
FalI AAGNNNNNCTT 6 cut(s) 23, 55, 339, 371, 357, 389
FaqI GGGAC 1 cut(s) 349
FatI CATG 5 cut(s) 113, 136, 145, 273, 505
FbaI TGATCA 1 cut(s) 432
FokI GGATG 1 cut(s) 130
FspBI CTAG 2 cut(s) 23, 449
HgaI GACGC 1 cut(s) 389
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 5 cut(s) 117, 140, 149, 277, 509
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 3 cut(s) 308, 408, 558
HphI GGTGA 3 cut(s) 442, 452, 525
Hpy188I TCNGA 5 cut(s) 195, 233, 241, 249, 467
Hpy188III TCNNGA 3 cut(s) 61, 356, 506
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 277, 489
HpyF3I CTNAG 3 cut(s) 47, 192, 374
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 5 cut(s) 117, 140, 149, 277, 509
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 2 cut(s) 241, 432
LguI GCTCTTC 1 cut(s) 204
LpnPI CCDG 5 cut(s) 57, 138, 263, 371, 398
LweI GCATC 3 cut(s) 111, 151, 349
MaeI CTAG 2 cut(s) 23, 449
MaeIII GTNAC 1 cut(s) 176
MalI GATC 2 cut(s) 243, 434
MboI GATC 2 cut(s) 241, 432
MboII GAAGA 3 cut(s) 21, 94, 221
MfeI CAATTG 1 cut(s) 480
MluCI AATT 5 cut(s) 54, 94, 167, 261, 480
MlyI GAGTC 3 cut(s) 317, 417, 552
MmeI TCCRAC 1 cut(s) 527
MnlI CCTC 7 cut(s) 42, 82, 148, 211, 255, 388, 487
MseI TTAA 2 cut(s) 78, 564
MspA1I CMGCKG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 386
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 386
NdeII GATC 2 cut(s) 241, 432
NlaIII CATG 5 cut(s) 117, 140, 149, 277, 509
NmuCI GTSAC 1 cut(s) 176
NspI RCATGY 1 cut(s) 277
PagI TCATGA 1 cut(s) 505
PasI CCCWGGG 1 cut(s) 385
PciSI GCTCTTC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PleI GAGTC 3 cut(s) 316, 416, 552
PpsI GAGTC 3 cut(s) 316, 416, 552
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 78, 564
Sau3AI GATC 2 cut(s) 241, 432
SchI GAGTC 3 cut(s) 317, 417, 552
ScrFI CCNGG 1 cut(s) 386
SetI ASST 8 cut(s) 24, 48, 112, 216, 222, 375, 450, 571
SfaNI GCATC 3 cut(s) 111, 151, 349
SfcI CTRYAG 1 cut(s) 472
SmlI CTYRAG 2 cut(s) 59, 215
SmoI CTYRAG 2 cut(s) 59, 215
Sse9I AATT 5 cut(s) 54, 94, 167, 261, 480
SsiI CCGC 1 cut(s) 258
SspMI CTAG 2 cut(s) 23, 449
StyD4I CCNGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 171
TasI AATT 5 cut(s) 54, 94, 167, 261, 480
TatI WGTACW 1 cut(s) 270
Tru1I TTAA 2 cut(s) 78, 564
Tru9I TTAA 2 cut(s) 78, 564
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 3 cut(s) 102, 180, 522
XapI RAATTY 2 cut(s) 54, 167
XceI RCATGY 1 cut(s) 277
XspI CTAG 2 cut(s) 23, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.