Rh4DG046500

Protein phosphatase 2C-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007891
Physical Location & Seq
Reverse (-)
7584521 .. 7588343
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG046500.1

Sequence Viewer

Length: 690 bp
ATGATGCCAGCAGTCTCACATGGGCATCATGTGGTTGAGGGTTATGATGAATTCGACTGTGACTATGTTGTGGCTCAGAGAGAGCAGATTGAAGAGCTTGAGGTGTGGCTTTTCGGAGTTTCCGATCCTCGGATTGGGGACGGAATTACCAAGTACATGCAGGCACATCTGTTTGATAAGAAGCCAAAAGAGTCTATCATAAAGGGAAAGAGCAACGAGACGATGAGAAAAGCATATCTTGATGCTAAAGCAAAGGTTAGAGACGCCCAGGGAACAGAGGAGACAAATGGAGTCGGTTCAGTTTCGGCAATGGTGATCAATGGTGAAAAGCTAGTATTAGCGAATATGGGAAACTACAGAGCAATTGCTTGCAGAGAGGGTTTGGCTCATGAAATAGGCATTAGGCACAAGCAATCACCCAAAATGAGTTGGACACATAGACTCTTTAAAGCAAACAATAAGCAATCTAAAAGCTCAGATCTTGTTGTTGGGGCCGAAAAGATTGAGAACGATATTGAATTTGTCATTTTAGCAAGCAGTGGCATATGGGAGGTGATGAAGTATCAAGAGGCTGTGAATCTCATAAGGCGCATTGAAAATCCAGAAATAGCTGCCGAGTGTTTGGCAAGTGAAGCTTCAAATAGAATGAGCAGAAGCAACATTTCTTGCTTGGTCATTCGATTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 map04011
Pfam Domains
Protein Families
PP2C

Protein Analysis

229

Amino Acids

25.7

Weight (kDa)

6.19

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 166 - 221 7.2e-10 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 2 cut(s) 50, 518
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 2 cut(s) 129, 134
AgsI TTSAA 4 cut(s) 92, 518, 596, 639
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 5 cut(s) 97, 331, 474, 611, 635
AluI AGCT 5 cut(s) 97, 331, 474, 611, 635
Alw26I GTCTC 4 cut(s) 19, 212, 255, 275
AlwI GGATC 1 cut(s) 119
AoxI GGCC 1 cut(s) 492
ApeKI GCWGC 1 cut(s) 611
ApoI RAATTY 2 cut(s) 50, 518
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 1 cut(s) 492
AsuHPI GGTGA 4 cut(s) 325, 335, 408, 565
BbvI GCAGC 1 cut(s) 598
BciT130I CCWGG 1 cut(s) 269
BclI TGATCA 1 cut(s) 315
BcoDI GTCTC 4 cut(s) 19, 212, 255, 275
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 355
BglII AGATCT 1 cut(s) 478
BisI GCNGC 1 cut(s) 612
BlsI GCNGC 1 cut(s) 613
Bme1390I CCNGG 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 492
BmiI GGNNCC 1 cut(s) 493
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 2 cut(s) 34, 232
BpuEI CTTGAG 1 cut(s) 119
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 128, 267, 268
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 134
Bse3DI GCAATG 1 cut(s) 315
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 3 cut(s) 128, 267, 268
BseLI CCNNNNNNNGG 2 cut(s) 129, 134
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 2 cut(s) 89, 489
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 1 cut(s) 598
BshFI GGCC 1 cut(s) 494
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 129, 134
BsmAI GTCTC 4 cut(s) 19, 212, 255, 275
BsmBI CGTCTC 2 cut(s) 212, 255
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 1 cut(s) 494
Bsp143I GATC 3 cut(s) 124, 315, 478
BspANI GGCC 1 cut(s) 494
BspCNI CTCAG 2 cut(s) 88, 488
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 493
BspPI GGATC 1 cut(s) 119
BspQI GCTCTTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 315
BssECI CCNNGG 3 cut(s) 128, 267, 268
BssMI GATC 3 cut(s) 124, 315, 478
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 264
BstC8I GCNNGC 4 cut(s) 9, 162, 370, 535
BstDEI CTNAG 2 cut(s) 75, 475
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 3 cut(s) 127, 318, 481
BstMAI GTCTC 4 cut(s) 19, 212, 255, 275
BstMBI GATC 3 cut(s) 124, 315, 478
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 598
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 1 cut(s) 494
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 9, 162, 370, 535
CciI TCATGA 1 cut(s) 388
CfoI GCGC 1 cut(s) 591
Cfr13I GGNCC 1 cut(s) 492
CseI GACGC 1 cut(s) 272
Csp6I GTAC 1 cut(s) 154
CviAII CATG 4 cut(s) 20, 29, 157, 389
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 2 cut(s) 75, 475
DpnI GATC 3 cut(s) 126, 317, 480
DpnII GATC 3 cut(s) 124, 315, 478
DraI TTTAAA 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 267
Esp3I CGTCTC 2 cut(s) 212, 255
FaeI CATG 4 cut(s) 23, 32, 160, 392
FalI AAGNNNNNCTT 4 cut(s) 222, 254, 619, 651
FaqI GGGAC 1 cut(s) 152
FatI CATG 4 cut(s) 19, 28, 156, 388
FauNDI CATATG 1 cut(s) 545
FbaI TGATCA 1 cut(s) 315
Fnu4HI GCNGC 1 cut(s) 612
Fsp4HI GCNGC 1 cut(s) 612
FspBI CTAG 1 cut(s) 332
GlaI GCGC 1 cut(s) 590
GluI GCNGC 1 cut(s) 612
HaeIII GGCC 1 cut(s) 494
HgaI GACGC 1 cut(s) 272
HhaI GCGC 1 cut(s) 591
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 4 cut(s) 23, 32, 160, 392
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 633
HinfI GANTC 4 cut(s) 191, 291, 441, 577
HphI GGTGA 4 cut(s) 325, 335, 408, 565
Hpy188I TCNGA 5 cut(s) 78, 116, 124, 132, 478
Hpy188III TCNNGA 4 cut(s) 239, 389, 566, 602
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 160, 372
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 2 cut(s) 75, 475
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 4 cut(s) 23, 32, 160, 392
HspAI GCGC 1 cut(s) 589
Ksp22I TGATCA 1 cut(s) 315
Kzo9I GATC 3 cut(s) 124, 315, 478
LguI GCTCTTC 1 cut(s) 87
LpnPI CCDG 5 cut(s) 21, 146, 254, 281, 615
Lsp1109I GCAGC 1 cut(s) 598
LweI GCATC 2 cut(s) 34, 232
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 59
MalI GATC 3 cut(s) 126, 317, 480
MboI GATC 3 cut(s) 124, 315, 478
MboII GAAGA 1 cut(s) 104
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 478
MluCI AATT 4 cut(s) 50, 144, 363, 518
MlyI GAGTC 3 cut(s) 200, 300, 435
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 7 cut(s) 31, 94, 138, 271, 370, 544, 562
MseI TTAA 1 cut(s) 447
MspR9I CCNGG 1 cut(s) 269
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 632
NdeI CATATG 1 cut(s) 545
NdeII GATC 3 cut(s) 124, 315, 478
NlaIII CATG 4 cut(s) 23, 32, 160, 392
NlaIV GGNNCC 1 cut(s) 493
NmeAIII GCCGAG 1 cut(s) 640
NmuCI GTSAC 1 cut(s) 59
NspI RCATGY 1 cut(s) 160
PagI TCATGA 1 cut(s) 388
PasI CCCWGGG 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 1 cut(s) 577
PkrI GCNGC 1 cut(s) 613
PleI GAGTC 3 cut(s) 199, 299, 435
PpsI GAGTC 3 cut(s) 199, 299, 435
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 1 cut(s) 493
PspPI GGNCC 1 cut(s) 492
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 1 cut(s) 612
Sau3AI GATC 3 cut(s) 124, 315, 478
Sau96I GGNCC 1 cut(s) 492
SchI GAGTC 3 cut(s) 200, 300, 435
ScrFI CCNGG 1 cut(s) 269
SetI ASST 8 cut(s) 99, 105, 258, 333, 476, 555, 613, 637
SfaNI GCATC 2 cut(s) 34, 232
SfcI CTRYAG 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 4 cut(s) 50, 144, 363, 518
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 59
TaqI TCGA 2 cut(s) 54, 679
TasI AATT 4 cut(s) 50, 144, 363, 518
TatI WGTACW 1 cut(s) 153
TfiI GAWTC 1 cut(s) 577
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 1 cut(s) 611
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 3 cut(s) 63, 405, 572
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 1 cut(s) 544
XapI RAATTY 2 cut(s) 50, 518
XceI RCATGY 1 cut(s) 160
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.