Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0015707

Physical Location & Seq

Genomic Coordinates Forward (+)

50198159 .. 50243277

Gene Structure 45119 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG209800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1596 bp

ATGGACGCTGTCGTCAGACGGGATCTATTATCAAGCAATCACAGGTCCCTCAATCGGGGGTGTTGGTGTCAGGTTGCAGGGGTGTTCGTGGAGGCGGGGTGGGTGAGGCTCAATTCTGGCAGGATGAAGTGGTGGGGCAGATGGGAGGTGGAGGTCGGATATGAGGTGGTTCTTCTCAGGCTGGTTGGACGAAGATGGCGGATGCTAGATGATCTGCTTTGTTTCGGGGATGGGAGTATTTCCAGATCCGATCGAGTCATGGTGGCTGGTAGTTCGGCGTCTTGGGTCAACTGGATTGGAGAAGAGCTGTTGCAGGTTTCGATCTACTTCGGTGGTTTGTCCGATGCTGATAGCAGACTGTTGCAGATGGTGGCTGGACGCAGGGGAGGTTTGGAGAAGCAGAGGAGAGAAGAGGTATCTCTGTATGATGATGTTTCCGAGGAACCAAAGGTTGGGGAGTTCATTGTAAAGTATCCCTCTTCTGAGTCACCGATATTCCACAAATGTTCTTGGAGACGTGATTCTCGAAATGACTCTGATGGTGTGCCAAGATTCCCACCTCTTGTGATCCCTAAAGATGCTGGAGCTGATCGAAATCCTCTGCACTCGGTAACTGGCTTACCACCTGTTCCACTCAATATTATAGTGGGATATGCTGTCCCTTTAATAGCAGATGCTCACGAGAGAGAGACACGCAAGCGAGAACCGGGATATGATGAGGTCGAGTACCGAAATATGATGAGAGGTCACACATGGCTGAATTTGGTTTTGTTTGCCTACAAGAAAGTTGATATTTCAGAACTCGAGAGTGTAATGGACAAACTGACCCAGGTTGCGCATTCCACGAGTCAATCTTTACCCACTGGCGCTGTTAAAATGTTCTACATACTGGTTATGTACAGAAGTGAGGTGTCTATGGAAAGACCTCCCTGGTCTTATGGTCTTCAAATTAAGAACATGTCTCATCTGATGTCTTATTCCCCACCCCTCATGGAGGTACCATTTGAAGAAGTTCCAGAACTTGTTGGTAGCCGTAGTGTATTCATCGGCAAGGGGCATGCATATATAGCTATGAGTCAGAAATTGGACATCAACTATAGTAGAACAAGAGAAAAATCGGTTGACTCCTCTCAGAGAGGAGCATCATCCAAAGCATGGAGGGAGAATGCAACTGGGTCTCTTTCAAGGTTGGTGCTGAGAGGTTTGACAAAGAATAGGCATATAGCATACGCCACAATTATGGAAGAGAAGAAGAAAGAATGGATTACATGCCTTATTCCTGTTAAAAGATCATCTAATCTACTCCTGGTGTTGAATATCATCATGGATGCCCATATCAACATTTCAGTGTCAGATTGCTGCAAAGACCATCTAACATCTCAGTTCTGTGAAGAGAATCTGACAGCTGCTCTCAGTAGAATGGAAGTAAACAGTCGTGCTCTTGGCGATGTAATGAAAGTTTGGAATAAGCATTATCAGTTGGCATGCACCGTAACGTTTGAAGCTGTCCAGGGGACATCATGCGAAGCTGGGATTAACCATCCAAATCAATACTTCTCTGACAGCCAAATAATTATGAAGTCAATGGAACATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0003887 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_primase_lrg

Protein Analysis

531

Amino Acids

60.26

Weight (kDa)

8.23

Isoelectric Point (pI)

55.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_primase_lrg	PF04104	462 - 519	7.6e-10	Eukaryotic and archaeal DNA primase, large subunit C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015707)

Species	Orthologous Gene IDs
malus_domestica	MD16G1122100.v1.1
pyrus_communis	pycom16g10460
rosa_chinensis	RchiOBHm_Chr4g0417231 RchiOBHm_Chr4g0417241 RchiOBHm_Chr4g0417251 RchiOBHm_Chr4g0417261
rosa_laevigata	RLG00000007921
rosa_multiflora	Rmu_sc0000646.1_g000011 Rmu_sc0002370.1_g000020
rosa_samantha	Rh4AG209800 Rh4CG220900 Rh4DG207500 Rh4DG207600 Rh4DG207700
rosa_wichuraiana	Rw4G017730 Rw4G017740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	11
Acc16I	TGCGCA	1 cut(s)	837
Acc36I	ACCTGC	1 cut(s)	304
Acc65I	GGTACC	1 cut(s)	997
AccB1I	GGYRCC	1 cut(s)	997
AccB7I	CCANNNNNTGG	2 cut(s)	452, 1155
AciI	CCGC	2 cut(s)	95, 199
AclI	AACGTT	1 cut(s)	1496
AclWI	GGATC	3 cut(s)	30, 240, 562
AcsI	RAATTY	1 cut(s)	760
AcyI	GRCGYC	1 cut(s)	278
AfaI	GTAC	3 cut(s)	728, 899, 999
AfiI	CCNNNNNNNGG	5 cut(s)	54, 55, 452, 994, 1155
AflIII	ACRYGT	1 cut(s)	957
AgsI	TTSAA	5 cut(s)	947, 1007, 1185, 1315, 1502
AjiI	CACGTC	1 cut(s)	518
AjnI	CCWGG	4 cut(s)	828, 929, 1305, 1509
AjuI	GAANNNNNNNTTGG	2 cut(s)	435, 467
AluBI	AGCT	6 cut(s)	307, 587, 1070, 1406, 1505, 1529
AluI	AGCT	6 cut(s)	307, 587, 1070, 1406, 1505, 1529
Alw21I	GWGCWC	1 cut(s)	1441
Alw26I	GTCTC	4 cut(s)	508, 683, 966, 1182
AlwI	GGATC	3 cut(s)	30, 240, 562
Ama87I	CYCGRG	1 cut(s)	803
ApeKI	GCWGC	2 cut(s)	1359, 1406
ApoI	RAATTY	1 cut(s)	760
Asp700I	GAANNNNTTC	1 cut(s)	1011
Asp718I	GGTACC	1 cut(s)	997
AspLEI	GCGC	2 cut(s)	838, 869
AspS9I	GGNCC	1 cut(s)	45
AsuC2I	CCSGG	1 cut(s)	708
AsuHPI	GGTGA	2 cut(s)	115, 480
AvaI	CYCGRG	1 cut(s)	803
AvaII	GGWCC	1 cut(s)	45
BanI	GGYRCC	1 cut(s)	997
BauI	CACGAG	2 cut(s)	680, 844
BbsI	GAAGAC	1 cut(s)	935
Bbv12I	GWGCWC	1 cut(s)	1441
BbvI	GCAGC	2 cut(s)	1346, 1393
BccI	CCATC	7 cut(s)	135, 189, 224, 361, 533, 1377, 1548
BceAI	ACGGC	1 cut(s)	1017
BciT130I	CCWGG	4 cut(s)	830, 931, 1307, 1511
BciVI	GTATCC	1 cut(s)	483
BcnI	CCSGG	1 cut(s)	708
BcoDI	GTCTC	4 cut(s)	508, 683, 966, 1182
BfaI	CTAG	1 cut(s)	206
BfmI	CTRYAG	1 cut(s)	1096
BfoI	RGCGCY	1 cut(s)	870
BfuAI	ACCTGC	1 cut(s)	304
BfuI	GTATCC	1 cut(s)	483
BisI	GCNGC	2 cut(s)	1360, 1407
BlsI	GCNGC	2 cut(s)	1361, 1408
Bme1390I	CCNGG	5 cut(s)	708, 830, 931, 1307, 1511
Bme18I	GGWCC	1 cut(s)	45
BmeT110I	CYCGRG	1 cut(s)	803
BmgBI	CACGTC	1 cut(s)	518
BmgT120I	GGNCC	1 cut(s)	45
BmiI	GGNNCC	3 cut(s)	47, 444, 999
BmrFI	CCNGG	5 cut(s)	708, 830, 931, 1307, 1511
BmrI	ACTGGG	1 cut(s)	1182
BmsI	GCATC	6 cut(s)	192, 334, 568, 664, 1151, 1318
BmuI	ACTGGG	1 cut(s)	1182
BpiI	GAAGAC	1 cut(s)	935
BpmI	CTGGAG	1 cut(s)	603
BpuMI	CCSGG	1 cut(s)	708
BsaBI	GATNNNNATC	1 cut(s)	594
BsaHI	GRCGYC	1 cut(s)	278
BsaI	GGTCTC	1 cut(s)	1182
BsaJI	CCNNGG	4 cut(s)	438, 828, 929, 1510
BsaXI	ACNNNNNCTCC	2 cut(s)	83, 113
Bsc4I	CCNNNNNNNGG	5 cut(s)	54, 55, 452, 994, 1155
Bse1I	ACTGG	5 cut(s)	296, 619, 868, 894, 1177
Bse8I	GATNNNNATC	1 cut(s)	594
BseBI	CCWGG	4 cut(s)	830, 931, 1307, 1511
BseDI	CCNNGG	4 cut(s)	438, 828, 929, 1510
BseGI	GGATG	6 cut(s)	129, 207, 235, 1145, 1333, 1540
BseJI	GATNNNNATC	1 cut(s)	594
BseLI	CCNNNNNNNGG	5 cut(s)	54, 55, 452, 994, 1155
BseMII	CTCAG	6 cut(s)	190, 474, 1145, 1187, 1394, 1426
BseNI	ACTGG	5 cut(s)	296, 619, 868, 894, 1177
BseRI	GAGGAG	3 cut(s)	418, 1117, 1152
BseXI	GCAGC	2 cut(s)	1346, 1393
BseYI	CCCAGC	1 cut(s)	1529
BsgI	GTGCAG	1 cut(s)	587
Bsh1285I	CGRYCG	1 cut(s)	253
BshNI	GGYRCC	1 cut(s)	997
BsiEI	CGRYCG	1 cut(s)	253
BsiHKAI	GWGCWC	1 cut(s)	1441
BsiHKCI	CYCGRG	1 cut(s)	803
BsiSI	CCGG	1 cut(s)	707
BslFI	GGGAC	3 cut(s)	31, 644, 1528
BslI	CCNNNNNNNGG	5 cut(s)	54, 55, 452, 994, 1155
BsmAI	GTCTC	4 cut(s)	508, 683, 966, 1182
BsmBI	CGTCTC	1 cut(s)	508
BsmFI	GGGAC	3 cut(s)	31, 644, 1528
BsmI	GAATGC	2 cut(s)	838, 1171
Bso31I	GGTCTC	1 cut(s)	1182
BsoBI	CYCGRG	1 cut(s)	803
Bsp1286I	GDGCHC	1 cut(s)	1441
Bsp1407I	TGTACA	1 cut(s)	897
Bsp143I	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
BspACI	CCGC	2 cut(s)	95, 199
BspCNI	CTCAG	6 cut(s)	189, 475, 1144, 1188, 1393, 1425
BspLI	GGNNCC	3 cut(s)	47, 444, 999
BspMI	ACCTGC	1 cut(s)	304
BspPI	GGATC	3 cut(s)	30, 240, 562
BspQI	GCTCTTC	1 cut(s)	297
BspT107I	GGYRCC	1 cut(s)	997
BspTNI	GGTCTC	1 cut(s)	1182
BsrGI	TGTACA	1 cut(s)	897
BsrI	ACTGG	5 cut(s)	296, 619, 868, 894, 1177
BssECI	CCNNGG	4 cut(s)	438, 828, 929, 1510
BssMI	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
BssNI	GRCGYC	1 cut(s)	278
BssSI	CACGAG	2 cut(s)	680, 844
Bst2BI	CACGAG	2 cut(s)	680, 844
Bst2UI	CCWGG	4 cut(s)	830, 931, 1307, 1511
Bst4CI	ACNGT	3 cut(s)	360, 1433, 1492
Bst6I	CTCTTC	5 cut(s)	297, 405, 484, 1239, 1386
BstACI	GRCGYC	1 cut(s)	278
BstAUI	TGTACA	1 cut(s)	897
BstC8I	GCNNGC	3 cut(s)	698, 1059, 1486
BstDEI	CTNAG	6 cut(s)	176, 483, 1131, 1196, 1380, 1412
BstENI	CCTNNNNNAGG	1 cut(s)	992
BstF5I	GGATG	6 cut(s)	129, 207, 235, 1145, 1333, 1540
BstH2I	RGCGCY	1 cut(s)	870
BstHHI	GCGC	2 cut(s)	838, 869
BstKTI	GATC	8 cut(s)	25, 214, 248, 253, 324, 570, 592, 1292
BstMAI	GTCTC	4 cut(s)	508, 683, 966, 1182
BstMBI	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
BstMCI	CGRYCG	1 cut(s)	253
BstMWI	GCNNNNNNNGC	1 cut(s)	1067
BstNI	CCWGG	4 cut(s)	830, 931, 1307, 1511
BstNSI	RCATGY	4 cut(s)	961, 1061, 1272, 1488
BstSCI	CCNGG	5 cut(s)	706, 828, 929, 1305, 1509
BstSFI	CTRYAG	1 cut(s)	1096
BstV1I	GCAGC	2 cut(s)	1346, 1393
BstV2I	GAAGAC	1 cut(s)	935
BstX2I	RGATCY	2 cut(s)	22, 245
BstXI	CCANNNNNNTGG	1 cut(s)	1240
BstYI	RGATCY	2 cut(s)	22, 245
BsuI	GTATCC	1 cut(s)	483
BtgZI	GCGATG	1 cut(s)	1461
BtrI	CACGTC	1 cut(s)	518
BtsCI	GGATG	6 cut(s)	129, 207, 235, 1145, 1333, 1540
BtsIMutI	CAGTG	2 cut(s)	861, 1353
BveI	ACCTGC	1 cut(s)	304
Cac8I	GCNNGC	3 cut(s)	698, 1059, 1486
CfoI	GCGC	2 cut(s)	838, 869
Cfr13I	GGNCC	1 cut(s)	45
CseI	GACGC	3 cut(s)	14, 267, 387
Csp6I	GTAC	3 cut(s)	727, 898, 998
CviQI	GTAC	3 cut(s)	727, 898, 998
DdeI	CTNAG	6 cut(s)	176, 483, 1131, 1196, 1380, 1412
DpnI	GATC	8 cut(s)	24, 213, 247, 252, 323, 569, 591, 1291
DpnII	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
DrdI	GACNNNNNNGTC	1 cut(s)	11
DseDI	GACNNNNNNGTC	1 cut(s)	11
Eam1104I	CTCTTC	5 cut(s)	297, 405, 484, 1239, 1386
EarI	CTCTTC	5 cut(s)	297, 405, 484, 1239, 1386
EciI	GGCGGA	1 cut(s)	214
Eco31I	GGTCTC	1 cut(s)	1182
Eco47I	GGWCC	1 cut(s)	45
Eco88I	CYCGRG	1 cut(s)	803
EcoNI	CCTNNNNNAGG	1 cut(s)	992
EcoO109I	RGGNCCY	1 cut(s)	45
EcoRII	CCWGG	4 cut(s)	828, 929, 1305, 1509
EcoT22I	ATGCAT	1 cut(s)	1063
Esp3I	CGTCTC	1 cut(s)	508
FaqI	GGGAC	3 cut(s)	31, 644, 1528
FauI	CCCGC	1 cut(s)	88
Fnu4HI	GCNGC	2 cut(s)	1360, 1407
FokI	GGATG	6 cut(s)	136, 214, 242, 1132, 1340, 1527
Fsp4HI	GCNGC	2 cut(s)	1360, 1407
FspBI	CTAG	1 cut(s)	206
FspI	TGCGCA	1 cut(s)	837
GlaI	GCGC	2 cut(s)	837, 868
GluI	GCNGC	2 cut(s)	1360, 1407
GsaI	CCCAGC	1 cut(s)	1533
GsuI	CTGGAG	1 cut(s)	603
HaeII	RGCGCY	1 cut(s)	870
HapII	CCGG	1 cut(s)	707
HgaI	GACGC	3 cut(s)	14, 267, 387
HhaI	GCGC	2 cut(s)	838, 869
Hin1I	GRCGYC	1 cut(s)	278
Hin6I	GCGC	2 cut(s)	836, 867
HinP1I	GCGC	2 cut(s)	836, 867
HincII	GTYRAC	2 cut(s)	289, 1123
HindII	GTYRAC	2 cut(s)	289, 1123
HinfI	GANTC	9 cut(s)	255, 485, 521, 533, 552, 847, 1075, 1124, 1396
HpaII	CCGG	1 cut(s)	707
HphI	GGTGA	2 cut(s)	115, 480
Hpy166II	GTNNAC	3 cut(s)	289, 1123, 1429
Hpy188III	TCNNGA	5 cut(s)	243, 525, 680, 805, 1016
Hpy8I	GTNNAC	3 cut(s)	289, 1123, 1429
HpyCH4III	ACNGT	3 cut(s)	360, 1433, 1492
HpyCH4IV	ACGT	2 cut(s)	517, 1496
HpyCH4V	TGCA	8 cut(s)	77, 313, 364, 604, 1061, 1169, 1362, 1488
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1067
HpyF3I	CTNAG	6 cut(s)	176, 483, 1131, 1196, 1380, 1412
HpySE526I	ACGT	2 cut(s)	517, 1496
Hsp92I	GRCGYC	1 cut(s)	278
HspAI	GCGC	2 cut(s)	836, 867
KpnI	GGTACC	1 cut(s)	1001
Kzo9I	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
LguI	GCTCTTC	1 cut(s)	297
LmnI	GCTCC	2 cut(s)	584, 1139
Lsp1109I	GCAGC	2 cut(s)	1346, 1393
LweI	GCATC	6 cut(s)	192, 334, 568, 664, 1151, 1318
MaeI	CTAG	1 cut(s)	206
MaeII	ACGT	2 cut(s)	517, 1496
MaeIII	GTNAC	4 cut(s)	486, 610, 746, 1492
MalI	GATC	8 cut(s)	24, 213, 247, 252, 323, 569, 591, 1291
MboI	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
MflI	RGATCY	2 cut(s)	22, 245
MhlI	GDGCHC	1 cut(s)	1441
MluCI	AATT	6 cut(s)	112, 760, 948, 1082, 1236, 1572
MlyI	GAGTC	6 cut(s)	264, 494, 527, 856, 1084, 1118
MmeI	TCCRAC	2 cut(s)	136, 166
Mph1103I	ATGCAT	1 cut(s)	1063
MroXI	GAANNNNTTC	1 cut(s)	1011
MseI	TTAA	6 cut(s)	665, 873, 951, 1284, 1536, 1594
MslI	CAYNNNNRTG	2 cut(s)	1238, 1346
MspA1I	CMGCKG	1 cut(s)	1406
MspI	CCGG	1 cut(s)	707
MspR9I	CCNGG	5 cut(s)	708, 830, 931, 1307, 1511
Mva1269I	GAATGC	2 cut(s)	838, 1171
MvaI	CCWGG	4 cut(s)	830, 931, 1307, 1511
MwoI	GCNNNNNNNGC	1 cut(s)	1067
NciI	CCSGG	1 cut(s)	708
NdeII	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
NlaIV	GGNNCC	3 cut(s)	47, 444, 999
NmuCI	GTSAC	2 cut(s)	486, 746
NsbI	TGCGCA	1 cut(s)	837
NsiI	ATGCAT	1 cut(s)	1063
NspI	RCATGY	4 cut(s)	961, 1061, 1272, 1488
PaeI	GCATGC	2 cut(s)	1061, 1488
PaeR7I	CTCGAG	1 cut(s)	803
PciI	ACATGT	1 cut(s)	957
PciSI	GCTCTTC	1 cut(s)	297
PcsI	WCGNNNNNNNCGW	1 cut(s)	523
PctI	GAATGC	2 cut(s)	838, 1171
PdmI	GAANNNNTTC	1 cut(s)	1011
PfeI	GAWTC	3 cut(s)	521, 552, 1396
PflFI	GACNNNGTC	1 cut(s)	8
PflMI	CCANNNNNTGG	2 cut(s)	452, 1155
PkrI	GCNGC	2 cut(s)	1361, 1408
Ple19I	CGATCG	1 cut(s)	253
PleI	GAGTC	6 cut(s)	263, 493, 527, 855, 1083, 1118
PpsI	GAGTC	6 cut(s)	263, 493, 527, 855, 1083, 1118
PpuMI	RGGWCCY	1 cut(s)	45
PscI	ACATGT	1 cut(s)	957
Psp1406I	AACGTT	1 cut(s)	1496
Psp5II	RGGWCCY	1 cut(s)	45
Psp6I	CCWGG	4 cut(s)	828, 929, 1305, 1509
PspFI	CCCAGC	1 cut(s)	1529
PspGI	CCWGG	4 cut(s)	828, 929, 1305, 1509
PspN4I	GGNNCC	3 cut(s)	47, 444, 999
PspPI	GGNCC	1 cut(s)	45
PspPPI	RGGWCCY	1 cut(s)	45
PsuI	RGATCY	2 cut(s)	22, 245
PsyI	GACNNNGTC	1 cut(s)	8
PvuI	CGATCG	1 cut(s)	253
PvuII	CAGCTG	1 cut(s)	1406
RsaI	GTAC	3 cut(s)	728, 899, 999
RsaNI	GTAC	3 cut(s)	727, 898, 998
RseI	CAYNNNNRTG	2 cut(s)	1238, 1346
SapI	GCTCTTC	1 cut(s)	297
SaqAI	TTAA	6 cut(s)	665, 873, 951, 1284, 1536, 1594
SatI	GCNGC	2 cut(s)	1360, 1407
Sau3AI	GATC	8 cut(s)	22, 211, 245, 250, 321, 567, 589, 1289
Sau96I	GGNCC	1 cut(s)	45
SchI	GAGTC	6 cut(s)	264, 494, 527, 856, 1084, 1118
ScrFI	CCNGG	5 cut(s)	708, 830, 931, 1307, 1511
SduI	GDGCHC	1 cut(s)	1441
SfaNI	GCATC	6 cut(s)	192, 334, 568, 664, 1151, 1318
SfcI	CTRYAG	1 cut(s)	1096
Sfr274I	CTCGAG	1 cut(s)	803
SinI	GGWCC	1 cut(s)	45
SlaI	CTCGAG	1 cut(s)	803
SmiMI	CAYNNNNRTG	2 cut(s)	1238, 1346
SmlI	CTYRAG	1 cut(s)	803
SmoI	CTYRAG	1 cut(s)	803
SphI	GCATGC	2 cut(s)	1061, 1488
Sse9I	AATT	6 cut(s)	112, 760, 948, 1082, 1236, 1572
SsiI	CCGC	2 cut(s)	95, 199
SspI	AATATT	1 cut(s)	640
SspMI	CTAG	1 cut(s)	206
StyD4I	CCNGG	5 cut(s)	706, 828, 929, 1305, 1509
TaaI	ACNGT	3 cut(s)	360, 1433, 1492
TaiI	ACGT	2 cut(s)	520, 1499
TaqI	TCGA	6 cut(s)	253, 320, 526, 592, 723, 804
TasI	AATT	6 cut(s)	112, 760, 948, 1082, 1236, 1572
TatI	WGTACW	1 cut(s)	897
TfiI	GAWTC	3 cut(s)	521, 552, 1396
Tru1I	TTAA	6 cut(s)	665, 873, 951, 1284, 1536, 1594
Tru9I	TTAA	6 cut(s)	665, 873, 951, 1284, 1536, 1594
TscAI	CASTG	2 cut(s)	868, 1353
TseFI	GTSAC	2 cut(s)	486, 746
TseI	GCWGC	2 cut(s)	1359, 1406
Tsp45I	GTSAC	2 cut(s)	486, 746
TspDTI	ATGAA	5 cut(s)	140, 451, 1033, 1469, 1592
TspRI	CASTG	2 cut(s)	868, 1353
Tth111I	GACNNNGTC	1 cut(s)	8
Van91I	CCANNNNNTGG	2 cut(s)	452, 1155
VpaK11BI	GGWCC	1 cut(s)	45
XagI	CCTNNNNNAGG	1 cut(s)	992
XapI	RAATTY	1 cut(s)	760
XceI	RCATGY	4 cut(s)	961, 1061, 1272, 1488
XhoI	CTCGAG	1 cut(s)	803
XmnI	GAANNNNTTC	1 cut(s)	1011
XspI	CTAG	1 cut(s)	206
Zsp2I	ATGCAT	1 cut(s)	1063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.