Rh4AG222100

methionine sulfoxide reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010744
Physical Location & Seq
Forward (+)
52131099 .. 52132485
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG222100.1

Sequence Viewer

Length: 411 bp
ATGGCTGGACCAGGTTCGGTTCACAAGACCGAGGAAGAGTGGCAGGCTATTCTCTCCCCTGAACAGTTCAGGATTCTACGCCAGAAAGGGACAGAGCCAAAATTCACTGGGGAATATGACAAGGTTTTCGAAGAAGGGGTTTATACTTGTGCTGGTTGTGGAACACCACTTTACAAGTCTTCAACCAAGTTTAACTCTGGATGTGGTTGGCCTGCATTCTATGAGGGTTTGCCTGGAGCCATTAATCGTTCTCCGGATCCGGATGGCAGAAGAACTGAGATTACCTGTGCAGCTTGTGGTGGTCACTTGGGACATGTTTTCAAATGGGAGGGGACTAAGGTGCCTACAGATGAACGCCACTGTGTCAACAGCATTTCGATTAAGCTTGTTCCAGAAAATTCTGCATCATGA

Protein Analysis

136

Amino Acids

14.87

Weight (kDa)

6.08

Isoelectric Point (pI)

54.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SelR PF01641 18 - 130 3.3e-44 SelR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010744)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04810 AT4G04810 AT4G04830 AT4G04830
fragaria_vesca FvH4_4g13910
malus_domestica MD13G1177800.v1.1
prunus_persica Prupe.1G147300_v2.0.a1
pyrus_communis pycom13g15410
rosa_chinensis RchiOBHm_Chr4g0395581 RchiOBHm_Chr4g0419881
rosa_laevigata RLG00000007797
rosa_multiflora Rmu_sc0000720.1_g000015 Rmu_sc0003990.1_g000001
rosa_roxburghii Rroxscaffold_5G00362640
rosa_rugosa Rorug04G0161000
rosa_samantha Rh4AG222100 Rh4BG224300 Rh4CG235400 Rh4DG221100
rosa_wichuraiana Rw4G019130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 340
AccIII TCCGGA 2 cut(s) 253, 259
AclWI GGATC 2 cut(s) 251, 264
AcsI RAATTY 2 cut(s) 101, 397
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 2 cut(s) 183, 322
AjnI CCWGG 2 cut(s) 10, 232
AluBI AGCT 2 cut(s) 293, 385
AluI AGCT 2 cut(s) 293, 385
AlwI GGATC 2 cut(s) 251, 264
Aor13HI TCCGGA 2 cut(s) 253, 259
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 2 cut(s) 101, 397
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AseI ATTAAT 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 8
AsuII TTCGAA 1 cut(s) 129
AvaII GGWCC 1 cut(s) 8
BamHI GGATCC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 302
BccI CCATC 1 cut(s) 257
BciT130I CCWGG 2 cut(s) 12, 234
BfmI CTRYAG 1 cut(s) 345
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 12, 234
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 3 cut(s) 238, 258, 342
BmrFI CCNGG 2 cut(s) 12, 234
BmrI ACTGGG 1 cut(s) 117
BmuI ACTGGG 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 171
BpmI CTGGAG 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 2 cut(s) 253, 259
Bse1I ACTGG 1 cut(s) 112
BseAI TCCGGA 2 cut(s) 253, 259
BseBI CCWGG 2 cut(s) 12, 234
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 2 cut(s) 206, 268
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 340
BsiSI CCGG 2 cut(s) 254, 260
BslFI GGGAC 3 cut(s) 103, 324, 346
BsmFI GGGAC 3 cut(s) 103, 324, 346
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 1 cut(s) 211
Bsp119I TTCGAA 1 cut(s) 129
Bsp13I TCCGGA 2 cut(s) 253, 259
Bsp143I GATC 1 cut(s) 256
BspANI GGCC 1 cut(s) 211
BspCNI CTCAG 1 cut(s) 268
BspEI TCCGGA 2 cut(s) 253, 259
BspHI TCATGA 1 cut(s) 407
BspLI GGNNCC 3 cut(s) 238, 258, 342
BspPI GGATC 2 cut(s) 251, 264
BspT104I TTCGAA 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 340
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 1 cut(s) 256
Bst2UI CCWGG 2 cut(s) 12, 234
Bst4CI ACNGT 2 cut(s) 66, 362
Bst6I CTCTTC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 45, 213
BstDEI CTNAG 2 cut(s) 276, 336
BstF5I GGATG 2 cut(s) 206, 268
BstKTI GATC 1 cut(s) 259
BstMBI GATC 1 cut(s) 256
BstNI CCWGG 2 cut(s) 12, 234
BstNSI RCATGY 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 10, 232
BstSFI CTRYAG 1 cut(s) 345
BstV1I GCAGC 1 cut(s) 302
BstV2I GAAGAC 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 1 cut(s) 211
BtsCI GGATG 2 cut(s) 206, 268
BtsIMutI CAGTG 2 cut(s) 105, 358
Cac8I GCNNGC 2 cut(s) 45, 213
CciI TCATGA 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 8
CsiI ACCWGGT 1 cut(s) 10
CviAII CATG 2 cut(s) 314, 408
CviJI RGCY 7 cut(s) 5, 47, 97, 211, 239, 293, 385
CviKI_1 RGCY 7 cut(s) 5, 47, 97, 211, 239, 293, 385
DdeI CTNAG 2 cut(s) 276, 336
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 8
EcoRII CCWGG 2 cut(s) 10, 232
FaeI CATG 2 cut(s) 317, 411
FaiI YATR 5 cut(s) 117, 144, 222, 315, 409
FaqI GGGAC 3 cut(s) 103, 324, 346
FatI CATG 2 cut(s) 313, 407
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 2 cut(s) 213, 275
Fsp4HI GCNGC 1 cut(s) 291
GluI GCNGC 1 cut(s) 291
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 1 cut(s) 211
HapII CCGG 2 cut(s) 254, 260
Hin1II CATG 2 cut(s) 317, 411
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 1 cut(s) 73
HpaII CCGG 2 cut(s) 254, 260
Hpy166II GTNNAC 2 cut(s) 22, 367
Hpy188III TCNNGA 6 cut(s) 70, 198, 254, 260, 392, 408
Hpy8I GTNNAC 2 cut(s) 22, 367
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 2 cut(s) 66, 362
HpyCH4V TGCA 3 cut(s) 215, 290, 404
HpyF3I CTNAG 2 cut(s) 276, 336
Hsp92II CATG 2 cut(s) 317, 411
Kpn2I TCCGGA 2 cut(s) 253, 259
Kzo9I GATC 1 cut(s) 256
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 1 cut(s) 302
MabI ACCWGGT 1 cut(s) 10
MaeIII GTNAC 1 cut(s) 302
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 4 cut(s) 47, 143, 171, 282
MflI RGATCY 1 cut(s) 256
MluCI AATT 2 cut(s) 101, 397
MnlI CCTC 3 cut(s) 25, 217, 322
MroI TCCGGA 2 cut(s) 253, 259
MseI TTAA 3 cut(s) 192, 243, 381
MspI CCGG 2 cut(s) 254, 260
MspR9I CCNGG 2 cut(s) 12, 234
Mva1269I GAATGC 1 cut(s) 215
MvaI CCWGG 2 cut(s) 12, 234
NdeII GATC 1 cut(s) 256
NlaIII CATG 2 cut(s) 317, 411
NlaIV GGNNCC 3 cut(s) 238, 258, 342
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 1 cut(s) 317
NspV TTCGAA 1 cut(s) 129
PagI TCATGA 1 cut(s) 407
PciI ACATGT 1 cut(s) 313
PctI GAATGC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 73
PkrI GCNGC 1 cut(s) 292
PscI ACATGT 1 cut(s) 313
PshBI ATTAAT 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 10, 232
PspGI CCWGG 2 cut(s) 10, 232
PspN4I GGNNCC 3 cut(s) 238, 258, 342
PspPI GGNCC 1 cut(s) 8
PsrI GAACNNNNNNTAC 2 cut(s) 265, 297
PsuI RGATCY 1 cut(s) 256
SaqAI TTAA 3 cut(s) 192, 243, 381
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 12, 234
SetI ASST 6 cut(s) 16, 126, 287, 295, 342, 387
SexAI ACCWGGT 1 cut(s) 10
SfcI CTRYAG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 129
SinI GGWCC 1 cut(s) 8
Sse9I AATT 2 cut(s) 101, 397
StyD4I CCNGG 2 cut(s) 10, 232
TaaI ACNGT 2 cut(s) 66, 362
TaqI TCGA 2 cut(s) 129, 377
TaqII GACCGA 1 cut(s) 44
TasI AATT 2 cut(s) 101, 397
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 3 cut(s) 192, 243, 381
Tru9I TTAA 3 cut(s) 192, 243, 381
TscAI CASTG 2 cut(s) 112, 365
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 366
TspRI CASTG 2 cut(s) 112, 365
VpaK11BI GGWCC 1 cut(s) 8
VspI ATTAAT 1 cut(s) 243
XapI RAATTY 2 cut(s) 101, 397
XceI RCATGY 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.