Rh4AG226600

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014605
Physical Location & Seq
Reverse (-)
52777406 .. 52778478
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG226600.1

Sequence Viewer

Length: 846 bp
ATGGATTCAACGGACGGCAACACGGCGGAGAAGGATGCAAGACCAAACCGGTCGATTGAAGGTTATGAAGTTATTGACGTTATAAAAGCCAAATTAGAAGAAGAGTGTCCCGATATCGTCTCGTGTGCTGATATAGTTGCTTTGGCAGCCAGAGATGCTGTTTCTTACCAAGTTCGTAGCTCCATTGTCACAATATGTTTTGGCATACAACAATGTATTTATGATAGGGATCGATTTGATATTAATGTTGATGATCATGAATATTTGGCATATGTGCAGTTTGGCAGGTCCATGTGGCAGGTTTTTGGAGGGAGGAAAGATGGAAGAGTTTCACTTGCCACGGAGGCTGCAAGGGACTTGCCTTCCGGAGGTGCAAACTACACCACACTCCAAGACCAATTTGCTGGTTTAGGGCTAAACTACATAGATCTTGTTGCCTTATCAGGGGCGCATACCATCGGAGTAGCACATTGCGTAGTATTTGCAAGGAGACTGAACTTCACAGGAAAAGGGGACATAGACCCCTCACTTGATCCAGCATATGCAGAGTTCTTGAAAACACAATGCCCCAGCAGACCTAATCCGGCTACCACCGTAGAGCTTGACGAAAATAGCTCACTTTCTTTCGACAGTAACTACTATGTGAGTCTGAGGCAGAACAAAGGCCTGCTCGGATCAGATGCTGCCCTGCTCACTGACCGGCGGGCGGCTTGGGCTGCAAGAAGATTCCAAAATTTCCATGTCTTCATGACTCGCTTTGGTAAGTCAATGATGAAAATGGGTGACATTGGAATCAAAACAGGAGATGATGGAGAAATTAGGAAGAATTGCAGAGTTATTAATTAA

Protein Analysis

281

Amino Acids

31.08

Weight (kDa)

5.32

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 5 - 244 3.3e-57 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39040
fragaria_vesca FvH4_4g16970
malus_domestica MD13G1175300.v1.1 MD16G1177000.v1.1
prunus_persica Prupe.1G143900_v2.0.a1
pyrus_communis pycom13g15120
rosa_chinensis RchiOBHm_Chr4g0420641
rosa_laevigata RLG00000007745
rosa_multiflora Rmu_sc0011080.1_g000008
rosa_roxburghii Rroxscaffold_5G00363180
rosa_rugosa Rorug04G0165100
rosa_samantha Rh4AG226600 Rh4BG229200 Rh4CG241500 Rh4DG224900
rosa_wichuraiana Rw4G019590 Rw4G019610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 83
Acc36I ACCTGC 2 cut(s) 276, 289
AccIII TCCGGA 1 cut(s) 365
AciI CCGC 3 cut(s) 26, 703, 707
AclWI GGATC 3 cut(s) 237, 527, 682
AcsI RAATTY 1 cut(s) 733
AfiI CCNNNNNNNGG 3 cut(s) 368, 444, 706
AgeI ACCGGT 1 cut(s) 48
AgsI TTSAA 3 cut(s) 9, 59, 556
AluBI AGCT 3 cut(s) 180, 601, 615
AluI AGCT 3 cut(s) 180, 601, 615
Alw26I GTCTC 2 cut(s) 124, 484
AlwI GGATC 3 cut(s) 237, 527, 682
AlwNI CAGNNNCTG 1 cut(s) 683
Aor13HI TCCGGA 1 cut(s) 365
AoxI GGCC 1 cut(s) 664
ApeKI GCWGC 4 cut(s) 146, 347, 683, 716
ApoI RAATTY 1 cut(s) 733
AseI ATTAAT 2 cut(s) 243, 840
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 328
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 794
AvaII GGWCC 1 cut(s) 288
BauI CACGAG 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 736
BbvI GCAGC 4 cut(s) 158, 334, 670, 703
BccI CCATC 3 cut(s) 314, 464, 803
BceAI ACGGC 2 cut(s) 31, 39
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 124, 484
BfuAI ACCTGC 2 cut(s) 276, 289
BglI GCCNNNNNGGC 1 cut(s) 344
BglII AGATCT 1 cut(s) 427
BisI GCNGC 5 cut(s) 147, 348, 684, 708, 717
BlsI GCNGC 5 cut(s) 148, 349, 685, 709, 718
Bme18I GGWCC 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 288
BmsI GCATC 3 cut(s) 25, 145, 670
BpiI GAAGAC 1 cut(s) 736
Bsa29I ATCGAT 1 cut(s) 232
BsaBI GATNNNNATC 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 339
BsaWI WCCGGW 2 cut(s) 48, 365
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 444, 706
Bse118I RCCGGY 2 cut(s) 48, 699
Bse3DI GCAATG 1 cut(s) 469
Bse8I GATNNNNATC 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 365
BseCI ATCGAT 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 339
BseGI GGATG 1 cut(s) 40
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 3 cut(s) 368, 444, 706
BseMI GCAATG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 641
BseXI GCAGC 4 cut(s) 158, 334, 670, 703
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 1 cut(s) 296
Bsh1285I CGRYCG 1 cut(s) 53
BshFI GGCC 1 cut(s) 666
BshTI ACCGGT 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 232
BsiEI CGRYCG 1 cut(s) 53
BsiSI CCGG 4 cut(s) 49, 366, 584, 700
BslFI GGGAC 3 cut(s) 93, 368, 527
BslI CCNNNNNNNGG 3 cut(s) 368, 444, 706
BsmAI GTCTC 2 cut(s) 124, 484
BsmBI CGTCTC 1 cut(s) 124
BsmFI GGGAC 3 cut(s) 93, 368, 527
BsnI GGCC 1 cut(s) 666
Bsp13I TCCGGA 1 cut(s) 365
Bsp143I GATC 5 cut(s) 229, 253, 427, 532, 674
BspACI CCGC 3 cut(s) 26, 703, 707
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 1 cut(s) 642
BspDI ATCGAT 1 cut(s) 232
BspEI TCCGGA 1 cut(s) 365
BspHI TCATGA 2 cut(s) 256, 747
BspMI ACCTGC 2 cut(s) 276, 289
BspPI GGATC 3 cut(s) 237, 527, 682
BsrDI GCAATG 1 cut(s) 469
BsrFI RCCGGY 2 cut(s) 48, 699
BssAI RCCGGY 2 cut(s) 48, 699
BssECI CCNNGG 1 cut(s) 339
BssMI GATC 5 cut(s) 229, 253, 427, 532, 674
BssSI CACGAG 1 cut(s) 121
Bst2BI CACGAG 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 595, 632
Bst6I CTCTTC 2 cut(s) 96, 319
BstC8I GCNNGC 2 cut(s) 668, 705
BstDEI CTNAG 1 cut(s) 650
BstDSI CCRYGG 1 cut(s) 339
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 40
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 5 cut(s) 232, 256, 430, 535, 677
BstMAI GTCTC 2 cut(s) 124, 484
BstMBI GATC 5 cut(s) 229, 253, 427, 532, 674
BstMCI CGRYCG 1 cut(s) 53
BstMWI GCNNNNNNNGC 5 cut(s) 146, 155, 344, 713, 716
BstV1I GCAGC 4 cut(s) 158, 334, 670, 703
BstV2I GAAGAC 1 cut(s) 736
BstX2I RGATCY 1 cut(s) 427
BstXI CCANNNNNNTGG 1 cut(s) 404
BstYI RGATCY 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 232
BsuRI GGCC 1 cut(s) 666
BsuTUI ATCGAT 1 cut(s) 232
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 693
BveI ACCTGC 2 cut(s) 276, 289
Cac8I GCNNGC 2 cut(s) 668, 705
CaiI CAGNNNCTG 1 cut(s) 683
CciI TCATGA 2 cut(s) 256, 747
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 2 cut(s) 48, 699
Cfr13I GGNCC 1 cut(s) 288
ClaI ATCGAT 1 cut(s) 232
CspAI ACCGGT 1 cut(s) 48
CviAII CATG 4 cut(s) 257, 292, 740, 748
DdeI CTNAG 1 cut(s) 650
DpnI GATC 5 cut(s) 231, 255, 429, 534, 676
DpnII GATC 5 cut(s) 229, 253, 427, 532, 674
Eam1104I CTCTTC 2 cut(s) 96, 319
EarI CTCTTC 2 cut(s) 96, 319
EciI GGCGGA 1 cut(s) 41
Eco147I AGGCCT 1 cut(s) 666
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoRV GATATC 1 cut(s) 115
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 4 cut(s) 260, 295, 743, 751
FaqI GGGAC 3 cut(s) 93, 368, 527
FatI CATG 4 cut(s) 256, 291, 739, 747
FauI CCCGC 1 cut(s) 696
FauNDI CATATG 2 cut(s) 271, 541
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 5 cut(s) 147, 348, 684, 708, 717
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 5 cut(s) 147, 348, 684, 708, 717
GlaI GCGC 1 cut(s) 450
GluI GCNGC 5 cut(s) 147, 348, 684, 708, 717
GsaI CCCAGC 1 cut(s) 573
HaeIII GGCC 1 cut(s) 666
HapII CCGG 4 cut(s) 49, 366, 584, 700
HhaI GCGC 1 cut(s) 451
Hin1II CATG 4 cut(s) 260, 295, 743, 751
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HinfI GANTC 5 cut(s) 5, 646, 726, 751, 792
HpaII CCGG 4 cut(s) 49, 366, 584, 700
HphI GGTGA 1 cut(s) 794
Hpy188I TCNGA 4 cut(s) 461, 651, 674, 679
Hpy188III TCNNGA 5 cut(s) 110, 257, 366, 553, 748
HpyAV CCTTC 3 cut(s) 25, 53, 372
HpyCH4III ACNGT 2 cut(s) 595, 632
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 8 cut(s) 38, 277, 350, 374, 485, 545, 719, 831
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 155, 344, 713, 716
HpyF3I CTNAG 1 cut(s) 650
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 4 cut(s) 260, 295, 743, 751
HspAI GCGC 1 cut(s) 449
Kpn2I TCCGGA 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 5 cut(s) 229, 253, 427, 532, 674
LmnI GCTCC 1 cut(s) 185
Lsp1109I GCAGC 4 cut(s) 158, 334, 670, 703
LweI GCATC 3 cut(s) 25, 145, 670
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 3 cut(s) 187, 632, 782
MalI GATC 5 cut(s) 231, 255, 429, 534, 676
MboI GATC 5 cut(s) 229, 253, 427, 532, 674
MboII GAAGA 6 cut(s) 110, 113, 336, 735, 736, 835
MflI RGATCY 1 cut(s) 427
MluCI AATT 6 cut(s) 92, 398, 733, 816, 826, 841
MlyI GAGTC 2 cut(s) 655, 745
MnlI CCTC 6 cut(s) 302, 306, 337, 362, 535, 645
MroI TCCGGA 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 328
MseI TTAA 3 cut(s) 243, 840, 844
MspI CCGG 4 cut(s) 49, 366, 584, 700
MwoI GCNNNNNNNGC 5 cut(s) 146, 155, 344, 713, 716
NdeI CATATG 2 cut(s) 271, 541
NdeII GATC 5 cut(s) 229, 253, 427, 532, 674
NlaIII CATG 4 cut(s) 260, 295, 743, 751
NmuCI GTSAC 2 cut(s) 187, 782
PacI TTAATTAA 1 cut(s) 844
PagI TCATGA 2 cut(s) 256, 747
PceI AGGCCT 1 cut(s) 666
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 3 cut(s) 5, 726, 792
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 5 cut(s) 148, 349, 685, 709, 718
PleI GAGTC 2 cut(s) 654, 745
PpsI GAGTC 2 cut(s) 654, 745
PshBI ATTAAT 2 cut(s) 243, 840
PsiI TTATAA 1 cut(s) 83
PspFI CCCAGC 1 cut(s) 569
PspPI GGNCC 1 cut(s) 288
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 1 cut(s) 427
SaqAI TTAA 3 cut(s) 243, 840, 844
SatI GCNGC 5 cut(s) 147, 348, 684, 708, 717
Sau3AI GATC 5 cut(s) 229, 253, 427, 532, 674
Sau96I GGNCC 1 cut(s) 288
SchI GAGTC 2 cut(s) 655, 745
SetI ASST 9 cut(s) 64, 81, 182, 290, 303, 373, 580, 603, 617
SfaNI GCATC 3 cut(s) 25, 145, 670
SinI GGWCC 1 cut(s) 288
Sse9I AATT 6 cut(s) 92, 398, 733, 816, 826, 841
SseBI AGGCCT 1 cut(s) 666
SsiI CCGC 3 cut(s) 26, 703, 707
SspI AATATT 1 cut(s) 263
StuI AGGCCT 1 cut(s) 666
TaaI ACNGT 2 cut(s) 595, 632
TaiI ACGT 1 cut(s) 81
TaqI TCGA 3 cut(s) 53, 232, 627
TasI AATT 6 cut(s) 92, 398, 733, 816, 826, 841
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 3 cut(s) 5, 726, 792
Tru1I TTAA 3 cut(s) 243, 840, 844
Tru9I TTAA 3 cut(s) 243, 840, 844
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 2 cut(s) 187, 782
TseI GCWGC 4 cut(s) 146, 347, 683, 716
Tsp45I GTSAC 2 cut(s) 187, 782
TspDTI ATGAA 4 cut(s) 81, 273, 736, 788
TspGWI ACGGA 2 cut(s) 26, 356
TspRI CASTG 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 288
VspI ATTAAT 2 cut(s) 243, 840
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 1 cut(s) 733
XmnI GAANNNNTTC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.