Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0015598

Physical Location & Seq

Genomic Coordinates Reverse (-)

54415742 .. 54419160

Gene Structure 3419 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG238400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGAACTCGTCAAAGCCTTATGTTCGACTCTCTGCAGCACGAGACAACTCAGGAGCTTGGAACCCACAACAGAAGCTTGCAGTTTCTAGTGCATCTTCACTTTCAGGTTCTCTTCCTATCCGAGCTCGTCTTACTCTAGATTCTGATTCTGACTCTGAAATCAACGGTGGTGATGAATTGGGTTATATTACTAGTAGTAGTAACATTAGTGTTGGTTCCGAGAGTGGGTTTGTGAGTGGGGAAGAGTTTGAAACGGCGTCAGAGAGGCCCTTTGTGGCGGTGGAGTCGGGTGAGGAATCCCTTGAAGAGAGTGGCTCTGTTGAAAAATATGAGGTTTTTTGGCCTTCTGTGTCTGACCCAGATGAATATGAAGAAGCTGTTGTGGGTAAAGTTATGGTGGGTGGTCCGATTTCGGAGGAGGTTAGGCCCATTGCAAATTTGTCATGGGAGAGTGATGGGAGTGATGCAGTGAGTGAGGAGACTGAAGAAGTGAGTGAAGATGAAGGTGGTGGATTGTTGGGTCATGTTAAGGCTCTGGTTTATGGGTTTGTGGAGCAGGTTGGTATTCCACCTAGAGTCAAGGTTTCTGCGGTTGAGGAAGGAGAGGAAGAGTCCTTGGTTGTTAAAGAATCAAGTTTTGTTGAAGGTGGAAATGGAGATTCTGTTCAAGTGGAATGCGGTGAGGATTGTAGTTTTGATACTGCTGAGTCCAGTGAAACGAAATCTGTTGAGTTGAATTTGAATGTATTGGAAGGTGGAGAGAAAGATAAGTATGTTGAAGAAGAGTTCTTTGTGCATGAAAGTTCCGTGAAATTGGATGAATTGGATGTTGAGGGTAACGAGAATCTGAGTATGGGAAATAGTCATATATCTGAAGCTGAGGAAGATAAAGTTGAATATAGTGATTCCTTTAACTCAATCACACCAAGGTCTGTCTTAGGTGAGAATGTGGCCACTGAACTCGAGATTGAGGATCCGATTGCTAATGGGAAGGACTTGGTTTTTACTGGAGATGCTGGTGTGAAATTTCAAGGCAGTGAAGCCAAAGTGGAAGTCCCTCTCAAGGAGAAGGGTTACACTATGGAACAGCACCCAACCAGCTTGGGTCTGTTTGTGGATAGTGTTGCACATGTGTTTCAGGTTTATGAGGTTGAAAAAAAAGTAGAAGAAGAACACAAGTTAGTGATGGGGAAAGGCTCCTCCGATTCCTTCATGAAGCAGTCTGAGCCGGCCACAGAATTAAAGGGTGTTCAGAATTCAGACTTGAAAGCCATTGTTAAAGGTCAAACTGGGGAATCCATTCAAGCTGAATGGACTGAAGATAATGGTCTTGGTGGTGTGGAAACCAAGGAACCAGATATTTTGGAGAAAGCCAAGAGTATGGCAGAAGAGTCTATAATGCCTTCCCCAAAAAGCAAAACGAGTGATGTTGATATTAGTGAAAGTTTTGGGGCATTGGATAGAGTCCATTCAGAATGTGATTTTGATGTTGAGGGGAGTGGAAATCAGATAACGGACTCCAATTCATATTCACAGCCAAATCCAATTCTGAAGATCGTATATGTGAGCAAGCGCAATTTGTCTGAAGCTGCTGGGGGTGATGGATATGTACTGTGTTCTGATTTCCTTGAATCAATCACACCAAGTTCCAGTGTGACCCATGAACACATTTTTGGTGGTGAAACCAAGGTAAAAAATTGTAAAATAGGCAGAAGATTCGCTGGAGTAACCGTAACTCACTTGGGTGAGGATGCCACCACCGGACTCAAGATTGAGGATCAGATTGCTATTGGGAAGGGCTTGGTGATGGCTGGAAGTGCTGGAGCTGTGCAATGCCAAGGTGATACAGCATGTGGAGCCAAACTGGAATTGTGCCTCAAGAAGAAGAATTTCCCTATAAAGCAGGACCAAACTAGCTTGGGTCTGTCTTTGATGAAGTGGGGTGGTGGTCTTCTTGTAACAACTGATATACTGTCTCGGTTCTCTGTTGGAAGTTCTAAAATAGCTGTTCATGTTGTATTGGACAACAAGCAGAGGGGAAAGATTGCTATAAGAACCAGCAACTCCGATAAAATCCCAAGCGCAGGGATTATTGGGATTCTCTCAATTGCAGTTTCAGTCTTCGGGTTCTTCCGTTCAAGATTCGGTGTTCAGAACTCAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003924 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0007186 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017038 GO:0017111 GO:0019867 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043021 GO:0043024 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055085 GO:0060089 GO:0061927 GO:0065002 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

721

Amino Acids

77.2

Weight (kDa)

4.51

Isoelectric Point (pI)

40.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOC159_MAD	PF11886	557 - 707	3.1e-39	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015598)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g18070
malus_domestica	MD16G1168600.v1.1
prunus_persica	Prupe.1G136400_v2.0.a1
pyrus_communis	pycom16g14200
rosa_chinensis	RchiOBHm_Chr4g0422301
rosa_laevigata	RLG00000007622
rosa_multiflora	Rmu_sc0013245.1_g000017 Rmu_sc0019605.1_g000001
rosa_roxburghii	Rroxscaffold_5G00364390
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238400 Rh4BG241500 Rh4CG253400 Rh4DG237100 Rh4DG237200
rosa_wichuraiana	Rw4G020630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	547
AciI	CCGC	3 cut(s)	278, 590, 678
AclWI	GGATC	3 cut(s)	968, 981, 1785
AcoI	YGGCCR	2 cut(s)	951, 1230
AcsI	RAATTY	5 cut(s)	436, 736, 1025, 1255, 1888
AcuI	CTGAAG	5 cut(s)	504, 894, 1338, 1571, 1605
AcyI	GRCGYC	1 cut(s)	257
AfaI	GTAC	1 cut(s)	1611
AfiI	CCNNNNNNNGG	3 cut(s)	225, 1063, 2084
AflIII	ACRYGT	1 cut(s)	1129
AhlI	ACTAGT	1 cut(s)	191
AjuI	GAANNNNNNNTTGG	2 cut(s)	1656, 1688
AleI	CACNNNNGTG	1 cut(s)	1743
AloI	GAACNNNNNNTCC	2 cut(s)	648, 680
Alw21I	GWGCWC	1 cut(s)	127
Alw26I	GTCTC	3 cut(s)	36, 473, 1980
AlwI	GGATC	3 cut(s)	968, 981, 1785
Ama87I	CYCGRG	1 cut(s)	962
AoxI	GGCC	5 cut(s)	266, 341, 425, 951, 1230
ApeKI	GCWGC	2 cut(s)	35, 1589
ApoI	RAATTY	5 cut(s)	436, 736, 1025, 1255, 1888
ArsI	GACNNNNNNTTYG	2 cut(s)	1038, 1070
Asp700I	GAANNNNTTC	2 cut(s)	1299, 1889
AspLEI	GCGC	2 cut(s)	1575, 2084
AspS9I	GGNCC	4 cut(s)	267, 404, 426, 1906
AsuHPI	GGTGA	9 cut(s)	182, 302, 692, 953, 1610, 1691, 1757, 1816, 1853
AvaI	CYCGRG	1 cut(s)	962
AvaII	GGWCC	2 cut(s)	404, 1906
BalI	TGGCCA	1 cut(s)	953
BamHI	GGATCC	1 cut(s)	973
BanII	GRGCYC	1 cut(s)	127
BauI	CACGAG	1 cut(s)	39
BbsI	GAAGAC	2 cut(s)	1943, 2113
Bbv12I	GWGCWC	1 cut(s)	127
BbvCI	CCTCAGC	1 cut(s)	879
BbvI	GCAGC	2 cut(s)	47, 1576
BccI	CCATC	4 cut(s)	449, 1180, 1595, 1801
BceAI	ACGGC	1 cut(s)	270
BcgI	CGANNNNNNTGC	2 cut(s)	1699, 1733
BcoDI	GTCTC	3 cut(s)	36, 473, 1980
BcuI	ACTAGT	1 cut(s)	191
BfaI	CTAG	5 cut(s)	87, 137, 192, 573, 1914
BfmI	CTRYAG	1 cut(s)	33
BfuAI	ACCTGC	1 cut(s)	547
BisI	GCNGC	2 cut(s)	36, 1590
BlsI	GCNGC	2 cut(s)	37, 1591
Bme18I	GGWCC	2 cut(s)	404, 1906
BmeT110I	CYCGRG	1 cut(s)	962
BmgT120I	GGNCC	4 cut(s)	267, 404, 426, 1906
BmiI	GGNNCC	6 cut(s)	62, 217, 975, 1198, 1353, 1858
BmrI	ACTGGG	1 cut(s)	1299
BmsI	GCATC	4 cut(s)	101, 454, 1003, 1741
BmuI	ACTGGG	1 cut(s)	1299
BpiI	GAAGAC	2 cut(s)	1943, 2113
BplI	GAGNNNNNCTC	2 cut(s)	299, 331
BpmI	CTGGAG	3 cut(s)	1029, 1743, 1842
Bpu10I	CCTNAGC	1 cut(s)	879
BpuEI	CTTGAG	4 cut(s)	1046, 1751, 1862, 2143
BsaHI	GRCGYC	1 cut(s)	257
BsaJI	CCNNGG	5 cut(s)	615, 926, 1347, 1686, 1837
BsaWI	WCCGGW	1 cut(s)	1760
BsaXI	ACNNNNNCTCC	4 cut(s)	648, 678, 1716, 1746
Bsc4I	CCNNNNNNNGG	3 cut(s)	225, 1063, 2084
Bse118I	RCCGGY	1 cut(s)	1228
Bse1I	ACTGG	5 cut(s)	711, 1012, 1294, 1650, 1869
Bse3DI	GCAATG	2 cut(s)	429, 1838
BseDI	CCNNGG	5 cut(s)	615, 926, 1347, 1686, 1837
BseGI	GGATG	3 cut(s)	823, 832, 1756
BseLI	CCNNNNNNNGG	3 cut(s)	225, 1063, 2084
BseMI	GCAATG	2 cut(s)	429, 1838
BseMII	CTCAG	5 cut(s)	63, 696, 839, 870, 1215
BseNI	ACTGG	5 cut(s)	711, 1012, 1294, 1650, 1869
BseRI	GAGGAG	3 cut(s)	431, 491, 1189
BseXI	GCAGC	2 cut(s)	47, 1576
BseYI	CCCAGC	1 cut(s)	1592
BshFI	GGCC	5 cut(s)	268, 343, 427, 953, 1232
BsiHKAI	GWGCWC	1 cut(s)	127
BsiHKCI	CYCGRG	1 cut(s)	962
BsiSI	CCGG	2 cut(s)	1229, 1761
BslFI	GGGAC	1 cut(s)	1040
BslI	CCNNNNNNNGG	3 cut(s)	225, 1063, 2084
BsmAI	GTCTC	3 cut(s)	36, 473, 1980
BsmFI	GGGAC	1 cut(s)	1040
BsmI	GAATGC	1 cut(s)	680
BsnI	GGCC	5 cut(s)	268, 343, 427, 953, 1232
BsoBI	CYCGRG	1 cut(s)	962
Bsp1286I	GDGCHC	1 cut(s)	127
Bsp143I	GATC	3 cut(s)	973, 1554, 1777
BspACI	CCGC	3 cut(s)	278, 590, 678
BspANI	GGCC	5 cut(s)	268, 343, 427, 953, 1232
BspCNI	CTCAG	5 cut(s)	62, 697, 840, 871, 1216
BspHI	TCATGA	1 cut(s)	1212
BspLI	GGNNCC	6 cut(s)	62, 217, 975, 1198, 1353, 1858
BspMAI	CTGCAG	1 cut(s)	37
BspMI	ACCTGC	1 cut(s)	547
BspPI	GGATC	3 cut(s)	968, 981, 1785
BsrDI	GCAATG	2 cut(s)	429, 1838
BsrFI	RCCGGY	1 cut(s)	1228
BsrI	ACTGG	5 cut(s)	711, 1012, 1294, 1650, 1869
BssAI	RCCGGY	1 cut(s)	1228
BssECI	CCNNGG	5 cut(s)	615, 926, 1347, 1686, 1837
BssMI	GATC	3 cut(s)	973, 1554, 1777
BssNI	GRCGYC	1 cut(s)	257
BssSI	CACGAG	1 cut(s)	39
BssT1I	CCWWGG	5 cut(s)	615, 926, 1347, 1686, 1837
Bst2BI	CACGAG	1 cut(s)	39
Bst4CI	ACNGT	4 cut(s)	167, 1614, 1732, 1974
Bst6I	CTCTTC	6 cut(s)	117, 237, 300, 603, 777, 1383
BstACI	GRCGYC	1 cut(s)	257
BstC8I	GCNNGC	3 cut(s)	78, 1230, 1571
BstDEI	CTNAG	6 cut(s)	49, 705, 848, 879, 937, 1224
BstF5I	GGATG	3 cut(s)	823, 832, 1756
BstHHI	GCGC	2 cut(s)	1575, 2084
BstKTI	GATC	3 cut(s)	976, 1557, 1780
BstMAI	GTCTC	3 cut(s)	36, 473, 1980
BstMBI	GATC	3 cut(s)	973, 1554, 1777
BstMWI	GCNNNNNNNGC	3 cut(s)	1225, 1817, 1856
BstNSI	RCATGY	2 cut(s)	1133, 1854
BstSFI	CTRYAG	1 cut(s)	33
BstV1I	GCAGC	2 cut(s)	47, 1576
BstV2I	GAAGAC	2 cut(s)	1943, 2113
BstX2I	RGATCY	1 cut(s)	973
BstXI	CCANNNNNNTGG	1 cut(s)	1381
BstYI	RGATCY	1 cut(s)	973
BsuRI	GGCC	5 cut(s)	268, 343, 427, 953, 1232
BtsCI	GGATG	3 cut(s)	823, 832, 1756
BtsI	GCAGTG	2 cut(s)	474, 1042
BtsIMutI	CAGTG	5 cut(s)	474, 718, 954, 1042, 1657
BveI	ACCTGC	1 cut(s)	547
Cac8I	GCNNGC	3 cut(s)	78, 1230, 1571
CciI	TCATGA	1 cut(s)	1212
CfoI	GCGC	2 cut(s)	1575, 2084
Cfr10I	RCCGGY	1 cut(s)	1228
Cfr13I	GGNCC	4 cut(s)	267, 404, 426, 1906
CseI	GACGC	1 cut(s)	246
Csp6I	GTAC	1 cut(s)	1610
CviAII	CATG	8 cut(s)	444, 524, 797, 1130, 1213, 1661, 1851, 2012
CviQI	GTAC	1 cut(s)	1610
DdeI	CTNAG	6 cut(s)	49, 705, 848, 879, 937, 1224
DpnI	GATC	3 cut(s)	975, 1556, 1779
DpnII	GATC	3 cut(s)	973, 1554, 1777
EaeI	YGGCCR	2 cut(s)	951, 1230
Eam1104I	CTCTTC	6 cut(s)	117, 237, 300, 603, 777, 1383
EarI	CTCTTC	6 cut(s)	117, 237, 300, 603, 777, 1383
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	5 cut(s)	615, 926, 1347, 1686, 1837
Eco24I	GRGCYC	1 cut(s)	127
Eco47I	GGWCC	2 cut(s)	404, 1906
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	5 cut(s)	504, 894, 1338, 1571, 1605
Eco88I	CYCGRG	1 cut(s)	962
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	1 cut(s)	267
EcoRI	GAATTC	1 cut(s)	1255
EcoT14I	CCWWGG	5 cut(s)	615, 926, 1347, 1686, 1837
EcoT38I	GRGCYC	1 cut(s)	127
ErhI	CCWWGG	5 cut(s)	615, 926, 1347, 1686, 1837
FaeI	CATG	8 cut(s)	447, 527, 800, 1133, 1216, 1664, 1854, 2015
FaqI	GGGAC	1 cut(s)	1040
FatI	CATG	8 cut(s)	443, 523, 796, 1129, 1212, 1660, 1850, 2011
Fnu4HI	GCNGC	2 cut(s)	36, 1590
FokI	GGATG	3 cut(s)	830, 839, 1763
FriOI	GRGCYC	1 cut(s)	127
Fsp4HI	GCNGC	2 cut(s)	36, 1590
FspBI	CTAG	5 cut(s)	87, 137, 192, 573, 1914
GlaI	GCGC	2 cut(s)	1574, 2083
GluI	GCNGC	2 cut(s)	36, 1590
GsaI	CCCAGC	1 cut(s)	1596
GsuI	CTGGAG	3 cut(s)	1029, 1743, 1842
HaeIII	GGCC	5 cut(s)	268, 343, 427, 953, 1232
HapII	CCGG	2 cut(s)	1229, 1761
HgaI	GACGC	1 cut(s)	246
HhaI	GCGC	2 cut(s)	1575, 2084
Hin1I	GRCGYC	1 cut(s)	257
Hin1II	CATG	8 cut(s)	447, 527, 800, 1133, 1216, 1664, 1854, 2015
Hin6I	GCGC	2 cut(s)	1573, 2082
HinP1I	GCGC	2 cut(s)	1573, 2082
HindIII	AAGCTT	1 cut(s)	74
HpaII	CCGG	2 cut(s)	1229, 1761
HphI	GGTGA	9 cut(s)	182, 302, 692, 953, 1610, 1691, 1757, 1816, 1853
Hpy188III	TCNNGA	8 cut(s)	51, 137, 964, 1213, 1768, 1879, 2139, 2160
HpyCH4III	ACNGT	4 cut(s)	167, 1614, 1732, 1974
HpyCH4V	TGCA	9 cut(s)	35, 80, 92, 434, 467, 796, 1127, 1831, 2111
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1225, 1817, 1856
HpyF3I	CTNAG	6 cut(s)	49, 705, 848, 879, 937, 1224
Hsp92I	GRCGYC	1 cut(s)	257
Hsp92II	CATG	8 cut(s)	447, 527, 800, 1133, 1216, 1664, 1854, 2015
HspAI	GCGC	2 cut(s)	1573, 2082
KroI	GCCGGC	1 cut(s)	1228
KroNI	GCCGGC	1 cut(s)	1230
Kzo9I	GATC	3 cut(s)	973, 1554, 1777
LmnI	GCTCC	5 cut(s)	53, 553, 1202, 1823, 1856
Lsp1109I	GCAGC	2 cut(s)	47, 1576
LweI	GCATC	4 cut(s)	101, 454, 1003, 1741
MaeI	CTAG	5 cut(s)	87, 137, 192, 573, 1914
MaeIII	GTNAC	7 cut(s)	200, 836, 1073, 1654, 1726, 1732, 1957
MalI	GATC	3 cut(s)	975, 1556, 1779
MboI	GATC	3 cut(s)	973, 1554, 1777
MfeI	CAATTG	1 cut(s)	2106
MflI	RGATCY	1 cut(s)	973
MhlI	GDGCHC	1 cut(s)	127
MlsI	TGGCCA	1 cut(s)	953
MluNI	TGGCCA	1 cut(s)	953
MmeI	TCCRAC	1 cut(s)	1969
Mox20I	TGGCCA	1 cut(s)	953
MroNI	GCCGGC	1 cut(s)	1228
MroXI	GAANNNNTTC	2 cut(s)	1299, 1889
MscI	TGGCCA	1 cut(s)	953
MseI	TTAA	5 cut(s)	528, 624, 912, 1241, 1278
MslI	CAYNNNNRTG	1 cut(s)	1743
Msp20I	TGGCCA	1 cut(s)	953
MspI	CCGG	2 cut(s)	1229, 1761
MunI	CAATTG	1 cut(s)	2106
Mva1269I	GAATGC	1 cut(s)	680
MwoI	GCNNNNNNNGC	3 cut(s)	1225, 1817, 1856
NaeI	GCCGGC	1 cut(s)	1230
NdeII	GATC	3 cut(s)	973, 1554, 1777
NgoMIV	GCCGGC	1 cut(s)	1228
NlaIII	CATG	8 cut(s)	447, 527, 800, 1133, 1216, 1664, 1854, 2015
NlaIV	GGNNCC	6 cut(s)	62, 217, 975, 1198, 1353, 1858
NmuCI	GTSAC	1 cut(s)	1654
NspI	RCATGY	2 cut(s)	1133, 1854
OliI	CACNNNNGTG	1 cut(s)	1743
PaeR7I	CTCGAG	1 cut(s)	962
PagI	TCATGA	1 cut(s)	1212
PciI	ACATGT	1 cut(s)	1129
PctI	GAATGC	1 cut(s)	680
PdiI	GCCGGC	1 cut(s)	1230
PdmI	GAANNNNTTC	2 cut(s)	1299, 1889
PkrI	GCNGC	2 cut(s)	37, 1591
PscI	ACATGT	1 cut(s)	1129
Psp124BI	GAGCTC	1 cut(s)	127
PspFI	CCCAGC	1 cut(s)	1592
PspN4I	GGNNCC	6 cut(s)	62, 217, 975, 1198, 1353, 1858
PspPI	GGNCC	4 cut(s)	267, 404, 426, 1906
PstI	CTGCAG	1 cut(s)	37
PsuI	RGATCY	1 cut(s)	973
RsaI	GTAC	1 cut(s)	1611
RsaNI	GTAC	1 cut(s)	1610
RseI	CAYNNNNRTG	1 cut(s)	1743
SacI	GAGCTC	1 cut(s)	127
SaqAI	TTAA	5 cut(s)	528, 624, 912, 1241, 1278
SatI	GCNGC	2 cut(s)	36, 1590
Sau3AI	GATC	3 cut(s)	973, 1554, 1777
Sau96I	GGNCC	4 cut(s)	267, 404, 426, 1906
SduI	GDGCHC	1 cut(s)	127
SfaNI	GCATC	4 cut(s)	101, 454, 1003, 1741
SfcI	CTRYAG	1 cut(s)	33
Sfr274I	CTCGAG	1 cut(s)	962
SinI	GGWCC	2 cut(s)	404, 1906
SlaI	CTCGAG	1 cut(s)	962
SmiMI	CAYNNNNRTG	1 cut(s)	1743
SmlI	CTYRAG	5 cut(s)	962, 1061, 1766, 1877, 2158
SmoI	CTYRAG	5 cut(s)	962, 1061, 1766, 1877, 2158
SpeI	ACTAGT	1 cut(s)	191
SsiI	CCGC	3 cut(s)	278, 590, 678
SspMI	CTAG	5 cut(s)	87, 137, 192, 573, 1914
SstI	GAGCTC	1 cut(s)	127
StyI	CCWWGG	5 cut(s)	615, 926, 1347, 1686, 1837
TaaI	ACNGT	4 cut(s)	167, 1614, 1732, 1974
TaqI	TCGA	2 cut(s)	25, 963
TatI	WGTACW	1 cut(s)	1609
Tru1I	TTAA	5 cut(s)	528, 624, 912, 1241, 1278
Tru9I	TTAA	5 cut(s)	528, 624, 912, 1241, 1278
TscAI	CASTG	5 cut(s)	474, 718, 961, 1042, 1657
TseFI	GTSAC	1 cut(s)	1654
TseI	GCWGC	2 cut(s)	35, 1589
Tsp45I	GTSAC	1 cut(s)	1654
TspGWI	ACGGA	3 cut(s)	796, 1529, 2123
TspRI	CASTG	5 cut(s)	474, 718, 961, 1042, 1657
VpaK11BI	GGWCC	2 cut(s)	404, 1906
XapI	RAATTY	5 cut(s)	436, 736, 1025, 1255, 1888
XbaI	TCTAGA	1 cut(s)	136
XceI	RCATGY	2 cut(s)	1133, 1854
XhoI	CTCGAG	1 cut(s)	962
XmnI	GAANNNNTTC	2 cut(s)	1299, 1889
XspI	CTAG	5 cut(s)	87, 137, 192, 573, 1914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.