Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0015598

Physical Location & Seq

Genomic Coordinates Reverse (-)

44642113 .. 44644276

Gene Structure 2164 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G020630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGGACTCGTCAAAGCCTTATGTTCGACTCTCTGCAGCACAAGACAACTCAGGAGCTTGGAACCCACAACAGAAGCTTGCAGTTTCTAGTTCATCTTCACTTTCAGGTTCTCTTCCTATCCGAGCTCGTCTTACTCTAGATTCTGATTCTGACTCTGAAATCAACGGTGGTCATGAATTGGGTTATATTACTAGTAGTAGTAACATTAGTGTTGGTTCCGAGAGTGGGTTTGTGAGTGGGGAAGAGTTTGAAACGGCGTCAGAGAGGCCCTTTGTGGCGGTGGAGTCGGATGAATATGAAGAAGCTGTTGTGGGTAAAGTTATGGTGGGTGGTCCGATTTCGGAGGAGGTTAGGCCCATTGCAAATTTGTCATGGGAGACTGATGGGAGTGATGCAGTGAGTGAGGAGACTGAAGAAGTGAGTGAAGATGAAGGTGGTGGATTGTTGGGTCATGTTAAGGCTCTGGTTTATGGGTTTGTGGAGCAGGTTGGTATTCCACCTAGAGTCAAGGTTTCTGAGGTTGAGGAAGGAGAAGAAGATGAGTCCTTGGTTGTTAAAGAATCAAGTTTTGTTGAAGGTGGAAATGGAGATTCTGTTCAAGTGGAATGCGGTGAGGATTGTAGTTTTGATACTGCTGAGTCCAGTGAAACGAAATCTGTTGAGTTGAATTTGAATGTATTGGAAGGTGTAGAGAAAGATAAGTATGTTGAAGAAGAGTTCTTTGTGCATGAAAGTTCCGTGAAATTGGATGAATTGGATGTTGAGGGTAACGAGAATCTGAGTATGGGAAATAGTCATATATCTGAAGCTGAGGAAGATAAAGTTGAATATAGTGATTCCTTTAACTCAATCACACCAAGGTCTGTCTTAGGTGAGAATGTGGCCACTGAACTCGAGATTGAGGATCCGATTGCTAATGGGAAGGACTTGGTTTTTACTGGAGATGCTGGTGTGAAATTTCAAGGCAGTGAAGCCAAAGTGGAAGTCCCTCTCAAGGAGAAGGGTTACACTATGGAACAGCACCCAACCAGCTTGGGTCTGTTTGTGGATAGTGTTGCACATGGGTTTCAGGTTTATGAGGTTGAAAAAAAAGTAGAAGAAGAACACAAGTTAGTGATGGGGAAAGGCTCCTCCGATTCCTTCATGAAGCAGTCTGAGCCGGCCACAGAATTAAAGGGTGTTCAGAATTCAGACTTGAAAGCCATTGTCAAAGGTCAAACTGGGGAATCCATTCAAGCTGAATGGACTGAAGATAATGGTCTTGGTGGTGTGGAAACCAAGGAACCAGATATTTTGGAGAAAGCCAAGAGCAGAAGATTCGCTGGAGTAACCGTAACTCACTTGGGTGAGGATGCCACCACAGGACTCAAGATTGAGGATCAGATTGCTATTGGGAAGGGCTTGGTGATGGCTGGAAGTGCTGGAGCTGTGCAATGCCAAGGTGATACAGCATGTGGAGCCAAACTGGAATTGTGCCTCAAGAAGAAGAATTTCCCTATAAAGCAGGACCAAACTAGCTTGGGTCTGTCTTTGATGAAGTGGGGTGGTGGTCTTCTTGTAACAACCGATATACTGTCTCGGTTCTCTGTTGGAAGTTCTAAAATAGCTGTTCATGTTGTATTGGACAACAAGCAGAGGGGAAAGATTGCTATAAGAACCAGCAACTCCGATAAAATCCCAAGCGCAGGGATTATTGGGATTCTCTCAATTGCAGTTTCAGTCTACGGGTTCTTCCGTTCAAGATTCGGTGTTCAGAACTCAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003924 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006605 GO:0006810 GO:0006886 GO:0007154 GO:0007165 GO:0007186 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009707 GO:0009941 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017038 GO:0017111 GO:0019867 GO:0023052 GO:0031090 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0038023 GO:0042170 GO:0042886 GO:0043021 GO:0043024 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051087 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0055085 GO:0060089 GO:0061927 GO:0065002 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

588

Amino Acids

62.94

Weight (kDa)

4.52

Isoelectric Point (pI)

39.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOC159_MAD	PF11886	438 - 574	1.1e-35	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015598)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g18070
malus_domestica	MD16G1168600.v1.1
prunus_persica	Prupe.1G136400_v2.0.a1
pyrus_communis	pycom16g14200
rosa_chinensis	RchiOBHm_Chr4g0422301
rosa_laevigata	RLG00000007622
rosa_multiflora	Rmu_sc0013245.1_g000017 Rmu_sc0019605.1_g000001
rosa_roxburghii	Rroxscaffold_5G00364390
rosa_rugosa	Rorug04G0179100
rosa_samantha	Rh4AG238400 Rh4BG241500 Rh4CG253400 Rh4DG237100 Rh4DG237200
rosa_wichuraiana	Rw4G020630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	475
AccI	GTMKAC	1 cut(s)	1722
AciI	CCGC	2 cut(s)	278, 609
AclWI	GGATC	3 cut(s)	899, 912, 1386
AcoI	YGGCCR	2 cut(s)	882, 1161
AcsI	RAATTY	5 cut(s)	364, 667, 956, 1186, 1489
AcuI	CTGAAG	3 cut(s)	432, 825, 1269
AcyI	GRCGYC	1 cut(s)	257
AfiI	CCNNNNNNNGG	3 cut(s)	225, 994, 1685
AhlI	ACTAGT	1 cut(s)	191
AleI	CACNNNNGTG	1 cut(s)	1344
AloI	GAACNNNNNNTCC	2 cut(s)	579, 611
Alw21I	GWGCWC	1 cut(s)	127
Alw26I	GTCTC	3 cut(s)	371, 401, 1581
AlwI	GGATC	3 cut(s)	899, 912, 1386
Ama87I	CYCGRG	1 cut(s)	893
AoxI	GGCC	4 cut(s)	266, 353, 882, 1161
ApeKI	GCWGC	1 cut(s)	35
ApoI	RAATTY	5 cut(s)	364, 667, 956, 1186, 1489
ArsI	GACNNNNNNTTYG	2 cut(s)	969, 1001
Asp700I	GAANNNNTTC	2 cut(s)	1230, 1490
AspLEI	GCGC	1 cut(s)	1685
AspS9I	GGNCC	4 cut(s)	267, 332, 354, 1507
AsuHPI	GGTGA	5 cut(s)	623, 884, 1358, 1417, 1454
AvaI	CYCGRG	1 cut(s)	893
AvaII	GGWCC	2 cut(s)	332, 1507
BalI	TGGCCA	1 cut(s)	884
BamHI	GGATCC	1 cut(s)	904
BanII	GRGCYC	1 cut(s)	127
BbsI	GAAGAC	1 cut(s)	1544
Bbv12I	GWGCWC	1 cut(s)	127
BbvCI	CCTCAGC	1 cut(s)	810
BbvI	GCAGC	1 cut(s)	47
BccI	CCATC	3 cut(s)	377, 1111, 1402
BceAI	ACGGC	1 cut(s)	270
BcgI	CGANNNNNNTGC	2 cut(s)	1300, 1334
BcoDI	GTCTC	3 cut(s)	371, 401, 1581
BcuI	ACTAGT	1 cut(s)	191
BfaI	CTAG	5 cut(s)	87, 137, 192, 501, 1515
BfmI	CTRYAG	1 cut(s)	33
BfuAI	ACCTGC	1 cut(s)	475
BisI	GCNGC	1 cut(s)	36
BlsI	GCNGC	1 cut(s)	37
Bme18I	GGWCC	2 cut(s)	332, 1507
BmeT110I	CYCGRG	1 cut(s)	893
BmgT120I	GGNCC	4 cut(s)	267, 332, 354, 1507
BmiI	GGNNCC	6 cut(s)	62, 217, 906, 1129, 1284, 1459
BmrI	ACTGGG	1 cut(s)	1230
BmsI	GCATC	3 cut(s)	382, 934, 1342
BmuI	ACTGGG	1 cut(s)	1230
BpiI	GAAGAC	1 cut(s)	1544
BpmI	CTGGAG	3 cut(s)	960, 1344, 1443
Bpu10I	CCTNAGC	1 cut(s)	810
BpuEI	CTTGAG	4 cut(s)	977, 1352, 1463, 1744
BsaHI	GRCGYC	1 cut(s)	257
BsaJI	CCNNGG	4 cut(s)	546, 857, 1278, 1438
BsaXI	ACNNNNNCTCC	4 cut(s)	579, 609, 1317, 1347
Bsc4I	CCNNNNNNNGG	3 cut(s)	225, 994, 1685
Bse118I	RCCGGY	1 cut(s)	1159
Bse1I	ACTGG	4 cut(s)	642, 943, 1225, 1470
Bse3DI	GCAATG	2 cut(s)	357, 1439
BseDI	CCNNGG	4 cut(s)	546, 857, 1278, 1438
BseGI	GGATG	4 cut(s)	295, 754, 763, 1357
BseLI	CCNNNNNNNGG	3 cut(s)	225, 994, 1685
BseMI	GCAATG	2 cut(s)	357, 1439
BseMII	CTCAG	6 cut(s)	63, 507, 627, 770, 801, 1146
BseNI	ACTGG	4 cut(s)	642, 943, 1225, 1470
BseRI	GAGGAG	3 cut(s)	359, 419, 1120
BseXI	GCAGC	1 cut(s)	47
BshFI	GGCC	4 cut(s)	268, 355, 884, 1163
BsiHKAI	GWGCWC	1 cut(s)	127
BsiHKCI	CYCGRG	1 cut(s)	893
BsiSI	CCGG	1 cut(s)	1160
BslFI	GGGAC	1 cut(s)	971
BslI	CCNNNNNNNGG	3 cut(s)	225, 994, 1685
BsmAI	GTCTC	3 cut(s)	371, 401, 1581
BsmFI	GGGAC	1 cut(s)	971
BsmI	GAATGC	1 cut(s)	611
BsnI	GGCC	4 cut(s)	268, 355, 884, 1163
BsoBI	CYCGRG	1 cut(s)	893
Bsp1286I	GDGCHC	1 cut(s)	127
Bsp143I	GATC	2 cut(s)	904, 1378
BspACI	CCGC	2 cut(s)	278, 609
BspANI	GGCC	4 cut(s)	268, 355, 884, 1163
BspCNI	CTCAG	6 cut(s)	62, 508, 628, 771, 802, 1147
BspHI	TCATGA	2 cut(s)	172, 1143
BspLI	GGNNCC	6 cut(s)	62, 217, 906, 1129, 1284, 1459
BspMAI	CTGCAG	1 cut(s)	37
BspMI	ACCTGC	1 cut(s)	475
BspPI	GGATC	3 cut(s)	899, 912, 1386
BsrDI	GCAATG	2 cut(s)	357, 1439
BsrFI	RCCGGY	1 cut(s)	1159
BsrI	ACTGG	4 cut(s)	642, 943, 1225, 1470
BssAI	RCCGGY	1 cut(s)	1159
BssECI	CCNNGG	4 cut(s)	546, 857, 1278, 1438
BssMI	GATC	2 cut(s)	904, 1378
BssNI	GRCGYC	1 cut(s)	257
BssT1I	CCWWGG	4 cut(s)	546, 857, 1278, 1438
Bst4CI	ACNGT	3 cut(s)	167, 1333, 1575
Bst6I	CTCTTC	3 cut(s)	117, 237, 708
BstACI	GRCGYC	1 cut(s)	257
BstC8I	GCNNGC	2 cut(s)	78, 1161
BstDEI	CTNAG	7 cut(s)	49, 516, 636, 779, 810, 868, 1155
BstF5I	GGATG	4 cut(s)	295, 754, 763, 1357
BstHHI	GCGC	1 cut(s)	1685
BstKTI	GATC	2 cut(s)	907, 1381
BstMAI	GTCTC	3 cut(s)	371, 401, 1581
BstMBI	GATC	2 cut(s)	904, 1378
BstMWI	GCNNNNNNNGC	3 cut(s)	1156, 1418, 1457
BstNSI	RCATGY	1 cut(s)	1455
BstSFI	CTRYAG	1 cut(s)	33
BstV1I	GCAGC	1 cut(s)	47
BstV2I	GAAGAC	1 cut(s)	1544
BstX2I	RGATCY	1 cut(s)	904
BstYI	RGATCY	1 cut(s)	904
BsuRI	GGCC	4 cut(s)	268, 355, 884, 1163
BtsCI	GGATG	4 cut(s)	295, 754, 763, 1357
BtsI	GCAGTG	2 cut(s)	402, 973
BtsIMutI	CAGTG	4 cut(s)	402, 649, 885, 973
BveI	ACCTGC	1 cut(s)	475
Cac8I	GCNNGC	2 cut(s)	78, 1161
CciI	TCATGA	2 cut(s)	172, 1143
CfoI	GCGC	1 cut(s)	1685
Cfr10I	RCCGGY	1 cut(s)	1159
Cfr13I	GGNCC	4 cut(s)	267, 332, 354, 1507
CseI	GACGC	1 cut(s)	246
CviAII	CATG	8 cut(s)	173, 372, 452, 728, 1061, 1144, 1452, 1613
DdeI	CTNAG	7 cut(s)	49, 516, 636, 779, 810, 868, 1155
DpnI	GATC	2 cut(s)	906, 1380
DpnII	GATC	2 cut(s)	904, 1378
EaeI	YGGCCR	2 cut(s)	882, 1161
Eam1104I	CTCTTC	3 cut(s)	117, 237, 708
EarI	CTCTTC	3 cut(s)	117, 237, 708
Ecl136II	GAGCTC	1 cut(s)	125
Eco130I	CCWWGG	4 cut(s)	546, 857, 1278, 1438
Eco24I	GRGCYC	1 cut(s)	127
Eco47I	GGWCC	2 cut(s)	332, 1507
Eco53kI	GAGCTC	1 cut(s)	125
Eco57I	CTGAAG	3 cut(s)	432, 825, 1269
Eco88I	CYCGRG	1 cut(s)	893
EcoICRI	GAGCTC	1 cut(s)	125
EcoO109I	RGGNCCY	1 cut(s)	267
EcoRI	GAATTC	1 cut(s)	1186
EcoT14I	CCWWGG	4 cut(s)	546, 857, 1278, 1438
EcoT38I	GRGCYC	1 cut(s)	127
ErhI	CCWWGG	4 cut(s)	546, 857, 1278, 1438
FaeI	CATG	8 cut(s)	176, 375, 455, 731, 1064, 1147, 1455, 1616
FaqI	GGGAC	1 cut(s)	971
FatI	CATG	8 cut(s)	172, 371, 451, 727, 1060, 1143, 1451, 1612
FblI	GTMKAC	1 cut(s)	1722
Fnu4HI	GCNGC	1 cut(s)	36
FokI	GGATG	4 cut(s)	302, 761, 770, 1364
FriOI	GRGCYC	1 cut(s)	127
Fsp4HI	GCNGC	1 cut(s)	36
FspBI	CTAG	5 cut(s)	87, 137, 192, 501, 1515
GlaI	GCGC	1 cut(s)	1684
GluI	GCNGC	1 cut(s)	36
GsuI	CTGGAG	3 cut(s)	960, 1344, 1443
HaeIII	GGCC	4 cut(s)	268, 355, 884, 1163
HapII	CCGG	1 cut(s)	1160
HgaI	GACGC	1 cut(s)	246
HhaI	GCGC	1 cut(s)	1685
Hin1I	GRCGYC	1 cut(s)	257
Hin1II	CATG	8 cut(s)	176, 375, 455, 731, 1064, 1147, 1455, 1616
Hin6I	GCGC	1 cut(s)	1683
HinP1I	GCGC	1 cut(s)	1683
HindIII	AAGCTT	1 cut(s)	74
HpaII	CCGG	1 cut(s)	1160
HphI	GGTGA	5 cut(s)	623, 884, 1358, 1417, 1454
Hpy166II	GTNNAC	1 cut(s)	1723
Hpy188III	TCNNGA	9 cut(s)	51, 137, 173, 895, 1144, 1369, 1480, 1740, 1761
Hpy8I	GTNNAC	1 cut(s)	1723
HpyAV	CCTTC	8 cut(s)	425, 521, 569, 677, 916, 994, 1150, 1390
HpyCH4III	ACNGT	3 cut(s)	167, 1333, 1575
HpyCH4V	TGCA	8 cut(s)	35, 80, 362, 395, 727, 1058, 1432, 1712
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1156, 1418, 1457
HpyF3I	CTNAG	7 cut(s)	49, 516, 636, 779, 810, 868, 1155
Hsp92I	GRCGYC	1 cut(s)	257
Hsp92II	CATG	8 cut(s)	176, 375, 455, 731, 1064, 1147, 1455, 1616
HspAI	GCGC	1 cut(s)	1683
KroI	GCCGGC	1 cut(s)	1159
KroNI	GCCGGC	1 cut(s)	1161
Kzo9I	GATC	2 cut(s)	904, 1378
LmnI	GCTCC	5 cut(s)	53, 481, 1133, 1424, 1457
Lsp1109I	GCAGC	1 cut(s)	47
LweI	GCATC	3 cut(s)	382, 934, 1342
MaeI	CTAG	5 cut(s)	87, 137, 192, 501, 1515
MaeIII	GTNAC	6 cut(s)	200, 767, 1004, 1327, 1333, 1558
MalI	GATC	2 cut(s)	906, 1380
MboI	GATC	2 cut(s)	904, 1378
MfeI	CAATTG	1 cut(s)	1707
MflI	RGATCY	1 cut(s)	904
MhlI	GDGCHC	1 cut(s)	127
MlsI	TGGCCA	1 cut(s)	884
MluNI	TGGCCA	1 cut(s)	884
MlyI	GAGTC	7 cut(s)	21, 146, 293, 513, 551, 647, 1359
MmeI	TCCRAC	2 cut(s)	267, 1570
Mox20I	TGGCCA	1 cut(s)	884
MroNI	GCCGGC	1 cut(s)	1159
MroXI	GAANNNNTTC	2 cut(s)	1230, 1490
MscI	TGGCCA	1 cut(s)	884
MseI	TTAA	4 cut(s)	456, 555, 843, 1172
MslI	CAYNNNNRTG	1 cut(s)	1344
Msp20I	TGGCCA	1 cut(s)	884
MspI	CCGG	1 cut(s)	1160
MunI	CAATTG	1 cut(s)	1707
Mva1269I	GAATGC	1 cut(s)	611
MwoI	GCNNNNNNNGC	3 cut(s)	1156, 1418, 1457
NaeI	GCCGGC	1 cut(s)	1161
NdeII	GATC	2 cut(s)	904, 1378
NgoMIV	GCCGGC	1 cut(s)	1159
NlaIII	CATG	8 cut(s)	176, 375, 455, 731, 1064, 1147, 1455, 1616
NlaIV	GGNNCC	6 cut(s)	62, 217, 906, 1129, 1284, 1459
NspI	RCATGY	1 cut(s)	1455
OliI	CACNNNNGTG	1 cut(s)	1344
PaeR7I	CTCGAG	1 cut(s)	893
PagI	TCATGA	2 cut(s)	172, 1143
PctI	GAATGC	1 cut(s)	611
PdiI	GCCGGC	1 cut(s)	1161
PdmI	GAANNNNTTC	2 cut(s)	1230, 1490
PkrI	GCNGC	1 cut(s)	37
PleI	GAGTC	7 cut(s)	21, 146, 292, 512, 550, 646, 1359
PpsI	GAGTC	7 cut(s)	21, 146, 292, 512, 550, 646, 1359
Psp124BI	GAGCTC	1 cut(s)	127
PspN4I	GGNNCC	6 cut(s)	62, 217, 906, 1129, 1284, 1459
PspPI	GGNCC	4 cut(s)	267, 332, 354, 1507
PstI	CTGCAG	1 cut(s)	37
PsuI	RGATCY	1 cut(s)	904
RseI	CAYNNNNRTG	1 cut(s)	1344
SacI	GAGCTC	1 cut(s)	127
SaqAI	TTAA	4 cut(s)	456, 555, 843, 1172
SatI	GCNGC	1 cut(s)	36
Sau3AI	GATC	2 cut(s)	904, 1378
Sau96I	GGNCC	4 cut(s)	267, 332, 354, 1507
SchI	GAGTC	7 cut(s)	21, 146, 293, 513, 551, 647, 1359
SduI	GDGCHC	1 cut(s)	127
SfaNI	GCATC	3 cut(s)	382, 934, 1342
SfcI	CTRYAG	1 cut(s)	33
Sfr274I	CTCGAG	1 cut(s)	893
SinI	GGWCC	2 cut(s)	332, 1507
SlaI	CTCGAG	1 cut(s)	893
SmiMI	CAYNNNNRTG	1 cut(s)	1344
SmlI	CTYRAG	5 cut(s)	893, 992, 1367, 1478, 1759
SmoI	CTYRAG	5 cut(s)	893, 992, 1367, 1478, 1759
SpeI	ACTAGT	1 cut(s)	191
SsiI	CCGC	2 cut(s)	278, 609
SspMI	CTAG	5 cut(s)	87, 137, 192, 501, 1515
SstI	GAGCTC	1 cut(s)	127
StyI	CCWWGG	4 cut(s)	546, 857, 1278, 1438
TaaI	ACNGT	3 cut(s)	167, 1333, 1575
TaqI	TCGA	2 cut(s)	25, 894
Tru1I	TTAA	4 cut(s)	456, 555, 843, 1172
Tru9I	TTAA	4 cut(s)	456, 555, 843, 1172
TscAI	CASTG	4 cut(s)	402, 649, 892, 973
TseI	GCWGC	1 cut(s)	35
TspGWI	ACGGA	2 cut(s)	727, 1724
TspRI	CASTG	4 cut(s)	402, 649, 892, 973
VpaK11BI	GGWCC	2 cut(s)	332, 1507
XapI	RAATTY	5 cut(s)	364, 667, 956, 1186, 1489
XbaI	TCTAGA	1 cut(s)	136
XceI	RCATGY	1 cut(s)	1455
XhoI	CTCGAG	1 cut(s)	893
XmiI	GTMKAC	1 cut(s)	1722
XmnI	GAANNNNTTC	2 cut(s)	1230, 1490
XspI	CTAG	5 cut(s)	87, 137, 192, 501, 1515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.